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Direct Polymerase Chain Reaction-Based Detection Of Cercospora Beticola In Field Soils, R. T. Lartey, T.C. Caesar-Tonthat, Andrew W. Lenssen, J. Eckhoff, S. L. Hanson, R. G. Evans
Direct Polymerase Chain Reaction-Based Detection Of Cercospora Beticola In Field Soils, R. T. Lartey, T.C. Caesar-Tonthat, Andrew W. Lenssen, J. Eckhoff, S. L. Hanson, R. G. Evans
Andrew W. Lenssen
Cercospora beticola, the causal agent of Cercospora leaf spot of sugar beet, survives as pseudostromata in infected sugar beet residues in the soil. Under optimal conditions, overwintering propagules germinate and produce conidia that are dispersed as primary inoculum to initiate infection in sugar beet. We developed a polymerase chain reaction (PCR) technique for rapid detection of C. beticola in field soils. Total DNA was first isolated from soil amended with C. beticola culture using the PowerSoil DNA Kit. The purified DNA was subjected to PCR in Extract-N-Amp PCR mix with CBACTIN primers over 35 cycles. The amplified products were resolved …