Plant Breeding and Genetics Commons^™

Distribution Of Genomic Variation In The Usda Soybean Germplasm Collection And Relationship With Phenotypic Variation, Nonoy Batiller Bandillo

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The USDA Soybean Germplasm Collection harbors a large stock of genetic diversity with potential to accelerate soybean cultivar development. The extent and nature of favorable alleles contained in the collection are not well known nor is the distribution of genetic variation and how it relates to phenotypic variation. The genotyping of the entire USDA Soybean Germplasm Collection marked the beginning of a systematic exploration of genetic diversity for genetic research and breeding. In this research, we conducted the first comprehensive analysis of population structure on the collection of ~14,400 soybean accessions [Glycine max (L.) Merr. and G. soja Siebold …

Genetic Analysis Of Soybean Mosaic Virus (Smv) Resistance Genes In Soybean [Glycine Max (L.) Merr.], Mariola Klepadlo

Graduate Theses and Dissertations

Soybean mosaic virus (SMV) causes the most serious viral disease in soybean worldwide. Seven SMV strains, G1 - G7, and three independent multi-allelic loci for SMV resistance, Rsv1, Rsv3, and Rsv4, have been identified. In the initial study, 299 soybean germplasm lines were genotyped for Rsv4 region, inoculated with SMV-G1 and G7 strains, and classified into several resistance groups. The Glyma.02g121400 locus was sequenced from ten soybean accessions, and alignment of the sequences revealed three SNPs displaying 100% polymorphic consistency when a soybean genotype carrying the Rsv4 gene was present. A cross between V94-5152 × Lee 68 was made to …

Genetic And Functional Analysis Of Host Genes Involved In Pathogenic And Symbiotic Legume-Microbe Interactions, Fang Tang

Theses and Dissertations--Plant and Soil Sciences

Legumes form symbiotic and pathogenic interactions with microbes. Understanding the molecular mechanisms underlying the legume-microbe interactions would help us to improve crop production in a sustainable manner. This thesis covers two independent research projects. The first project was to study the role of alternative splicing in RCT1-mediated disease resistance. RCT1 is a TIR-NBS-LRR-type plant resistance (R) gene in Medicago truncatula that confers broad-spectrum resistance to Colletotrichum trifolii, a fungal pathogen that causes anthracnose disease in Medicago. RCT1 undergoes alternative splicing at both coding and 3'-untranslated regions, thereby producing multiple transcript variants in its expression profile. …

Identification Of Quantitative Trait Loci / Genes For Low-Stachyose And High-Sucrose Content In Soybean Seeds, Sandra Liliana Florez Palacios

Graduate Theses and Dissertations

Seed carbohydrate content is an important aspect in breeding for food-grade soybeans commercialized in the soyfood market. Sucrose and stachyose are the primary carbohydrates in soybean seed. Sucrose affects the quality and taste of various soyfoods such as tofu, soymilk, and natto; however, consumption of soy-based products with high stachyose concentrations can cause diarrhea and flatulence. A mutant line (V99-5089) with high-sucrose and low-stachyose has been identified. The objectives of this research were: 1) to identify sucrose QTLs in a F2-derived mapping population; 2) to investigate the genetic relationship between two low-stachyose sources, V99-5089 and the germplasm line PI200508; and …

Evaluation Of Constitutive Iron Reductase (Atfro2) Expression On Mineral Accumulation And Distribution In Soybean (Glycine Max. L), Marta W. Vasconcelos, Thomas E. Clemente, Michael A. Grusak

Center for Plant Science Innovation: Faculty Publications

Iron is an important micronutrient in human and plant nutrition. Adequate iron nutrition during crop production is central for assuring appropriate iron concentrations in the harvestable organs, for human food or animal feed. The whole-plant movement of iron involves several processes, including the reduction of ferric to ferrous iron at several locations throughout the plant, prior to transmembrane trafficking of ferrous iron. In this study, soybean plants that constitutively expressed the AtFRO2 iron reductase gene were analyzed for leaf iron reductase activity, as well as the effect of this transgene's expression on root, leaf, pod wall, and seed mineral concentrations. …

Genome-Wide Association Mapping Identifies Qtls And Candidate Genes For Salt Tolerance In Soybean, Lei Huang

Graduate Theses and Dissertations

Salinity is a common abiotic stress causing soybean yield loss worldwide. Use of tolerant cultivars is an effective and economic approach to coping with this stress. Toward this, research is needed to identify salt-tolerant germplasm and better understand the genetic and molecular basis of salt tolerance in soybean. The objectives of this study were to identify salt-tolerant genotypes, to search for SNPs and QTLs associated with salt tolerance, and to identify candidate salt tolerance genes. A total of 192 diverse soybean lines and cultivars were screened for salt tolerance in the greenhouse based on visual leaf scorch scores after 15 …

Nutrient Contents, Color, Texture, And Sensory Evaluation Of 12 Arkansas Grown Soybean Cultivars In Canned Products, Quyen Nguyen, Navam Hettiarachchy, Srinivas J. Rayaprolu

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Soybean was introduced in the U.S. in the 1800s, and it has been proven to have several health benefits. New cultivars of soybeans with varying hull colors have been developed using plantbreeding technology. Canning is one of the effective processing methods to extend the shelf life of products. However, very little information is available on canned soybeans. This research studied the composition of 12 soybean cultivars including two cultivars with brown seed coat (R08-4014 and R09-349), three cultivars with black seed coat (R07-1927, R07-10396, and R09-345), and seven regular cultivars with yellow seed coat (R05-1772, R05-4969, R07-2001, R08-4005, R08- 4006, …

The Identification Of Quantitative Trait Loci For Sucrose And Stachyose Content In Soybean Seed, Ailan Zeng

Graduate Theses and Dissertations

Sucrose is a desirable sugar in soybean seed that affects the quality and taste of soyfoods, while stachyose is a non-digestible sugar that induces flatulence in non-ruminant animals. Therefore, soybean cultivars with high sucrose and/or low stachyose would be valuable for soyfood and meal markets. The objectives of this study were to identify quantitative trait loci (QTL) or genes associated with seed sucrose and stachyose content using simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers. A low sucrose line, MFS-553, was crossed with a high sucrose plant introduction, PI 243545, to develop the sucrose QTL mapping population. A …

Detection Of Soybean Seed Protein Qtls Using Selective Genotyping, Piyaporn Phansak

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A quantitative trait locus (QTL) is a statically defined location of a gene governing that trait. QTL identification is the first step towards using marker-assisted selection (MAS) to introgress desirable QTL alleles into elite high-yield cultivars. Hundreds of high protein plant introductions (PIs) exist in the USDA germplasm collection and are a source of high protein alleles. Although 86 protein QTLs are currently listed in SoyBase, many are likely repeat discoveries of the same QTL(s), given the typical +/- 10 cM confidence intervals associated with QTL positions. Six germplasm accessions of maturity groups (MGs) II to IV that exhibited high …

Crop Updates 2005 - Cereals, Darshan Sharma, Brenda Shackley, Mohammad Amjad, Christine M. Zaicou-Kunesch, Wal Anderson, D. Tennant, Steve Penny Jr, Ben Curtis, Veronika Reck, Richard Richards, Gavin Knell, Steve Curtin, David Sermon, Alan Bedggood, T. B. Biddulph, T. L. Setter, J. A. Plummer, D. J. Mares, H. Khabaz-Saberi, I. Waters, G. Mcdonald, Harmohinder Dhammu, David Nicholson, Chris Roberts, Stephen Loss, Ross Brennan, Kithsiri Jayasena, Eddy Pol, Simon Teakle, Rob Loughman, Ciara Beard, Blakely Paynter, K. Tanaka, G. Poulish, A. Smith, Paul Damon, Zed Rengel, Lionel Martin, Narelle Hill, Ron Mctaggart, Ray Tugwell, Geoff J. Thomas, Manisha Shankar, John Majewski, D. Foster, H. Golzar, J. Piotrowski, Rob Grima, Jeff Russell, Angie Roe, Vivien A. Vanstone, Sean Kelly, Helen Hunter, Mena C. Gilchrist, C. R. Newman, Svetlana Micic, Phil Michael, Gabrielle Coupland, Ern Kostas, Mohammed Hamza, Tina Botwright Acuña, Len Wade, Ingrid Richardson

Crop Updates

This session covers thirty six papers from different authors:

WHEAT AGRONOMY

1. Optimum sowing time of new wheat varieties in Western Australia, Darshan Sharma, Brenda Shackley, Mohammad Amjad, Christine M. Zaicou-Kunesch and Wal Anderson, Department of Agriculture

2. Wheat varieties updated in ‘Flowering Calculator’: A model predicting flowering time, B. Shackley, D. Tennant, D. Sharma and C.M. Zaicou-Kunesch, Department of Agriculture

3. Plant populations for wheat varieties, Christine M. Zaicou-Kunesch, Wal Anderson, Darshan Sharma, Brenda Shackley and Mohammad Amjad, Department of Agriculture

4. New wheat cultivars response to fertiliser nitrogen in four major agricultural regions of Western Australia, Mohammad Amjad, …

Conversion Of Aflp Bands Into High-Throughput Dna Markers, K. Meksem, E. Ruben, D. L. Hyten, K. Triwitayakorn, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

The conversion of AFLP bands into polymorphic sequence-tagged-site (STS) markers is necessary for high-throughput genotype scoring. Technical hurdles that must be overcome arise from genome complexity (particularly sequence duplication), from the low-molecular- weight nature of the AFLP bands and from the location of the polymorphism within the AFLP band. We generated six STS markers from ten AFLP bands (four AFLPs were from co-dominant pairs of bands) in soybean (Glycine max). The markers were all linked to one of two loci, rhg1 on linkage group G and Rhg4 on linkage group A2, that confer resistance to the soybean cyst nematode (Heterodera …

Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Large DNA insert libraries in binary T-DNA vectors can assist in the isolation of the gene(s) under-lying a quantitative trait locus (QTL). Binary vectors facilitate the transfer of large-insert DNA fragments containing a QTL from E. coli to Agrobacterium sp. and then to plants. We constructed two soybean large-insert libraries from cv. Forrest in the pCLD04541 (V41) binary vector after partial digestion of genomic high-molecular-weight DNA with BamHI or HindIII. The libraries contain 76,800 clones with an average insert size of 125 kb, and therefore represent 9.5-fold haploid genome equivalents. Colony hybridization using a chloroplast-specific probe infers that …