Plant Breeding and Genetics Commons^™

Soybean Genetics, Genomics, And Breeding For Improving Nutritional Value And Reducing Antinutritional Traits In Food And Feed, William M. Singer, Yi-Chen Lee, Zachary Shea, Caio Canella Vieira, Dongho Lee, Xiaoying Li, Mia Cunicelli, Shaila S. Kadam, Mohammad Amir Waseem Khan, Grover Shannon, M. A. Rouf Mian, Henry T. Nguyen, Bo Zhang

Agriculture Faculty Publications

Soybean [Glycine max(L.) Merr.] is a globally important crop due to its valuable seed composition, versatile feed, food, and industrial end-uses, and consistent genetic gain. Successful genetic gain in soybean has led to widespread adaptation and increased value for producers, processors, and consumers. Specific focus on the nutritional quality of soybean seed composition for food and feed has further elucidated genetic knowledge and bolstered breeding progress. Seed components are historical and current targets for soybean breeders seeking to improve nutritional quality of soybean. This article reviews genetic and genomic foundations for improvement of nutritionally important traits, such as protein and …

Identifying Genetic Sources Of Anthracnose Resistance In Global Sorghum Lines, Mary-Frances Behnke

All Theses

Anthracnose of sorghum (causal agent: Colletotrichum sublineola) is the most detrimental disease of sorghum (Sorghum bicolor) worldwide, significantly reducing grain yield. This study includes a literature review of the disease and a meta-analysis of genome-wide association studies (GWAS) of anthracnose-resistance in sorghum as means to elucidate genetic sources of the trait. The meta-analysis includes genotypic and phenotypic data from 5 unique studies and 1,071 sorghum lines. Original genotyping-by-sequencing data from were obtained primarily from National Center for Biotechnology Information’s Sequence Read Archive (NCBI-SRA) and prepared and processed using the Tassel 5 GBS v2. pipeline. Phenotypic data were …

Stabilitas Hasil Dan Adaptabilitas Galur-Galur Harapan Kacang Bogor Di Tiga Lokasi, Darmawan Saptadi, Descha Giatri Cahyaningrum, Noer Rahmi Ardiarini, Budi Waluyo

Jurnal Kultivasi

Kacang Bogor (Vigna subterranea (L.) Verdcourt) potensial dikembangkan sebagai komoditi pangan rendah lemak. Pengembangan dan peningkatan hasil komoditas ini dapat dilakukan melalui penyediaan varietas unggul. Tujuan penelitian ini ialah untuk mengetahui stabilitas dan adaptabilitas hasil enam galur harapan kacang Bogor, yaitu GSG 2.1.1, GSG 2.5, GSG 1.5, CCC 1.4.1, PWBG 5.3.1, dan BBL 6.1.1. Penelitian dilakukan di tiga lokasi yang memiliki karakteristik ketinggian tempat, kondisi lahan, dan musim tanam berbeda. Percobaan menggunakan rancangan acak kelompok dengan tiga ulangan yang dilanjutkan dengan analisis varians gabungan. Analisis regresi digunakan untuk menentukan stabilitas dan adaptabilitas hasil berdasarkan Eberhart-Russell dan Finlay-Wilkinson. Hasil penelitian menunjukkan …

Resistance To Aflatoxin Accumulation In Maize Mediated By Host-Induced Gene Silencing Of Aspergillus Flavus Alkaline Protease And O-Methyltransferase-A Genes, Olanike Omotola Omolehin

LSU Doctoral Dissertations

Aspergillus flavus is a soil-borne fungal pathogen that infects maize and produces aflatoxins. In the current study, portions of the alkaline protease (alk) and the O-methyl transferase (omtA) genes, which are key in A. flavus virulence and aflatoxin biosynthesis, respectively, were targeted for suppression through an RNAi (RNA interference) approach known as Host-Induced Gene Silencing (HIGS). Separate RNAi vectors were designed to carry regions of the alk and omtA gene fragments (Alk-RNAi and OmtA-RNAi) and introduced into B104 maize zygotic embryos. Eight and six transformation events were positive for the alk and omtA transgene, respectively. …

Improving Breeding Program Efficiency And Genetic Gain Through The Implementation Of Genomic Selection In Diverse Wheat Germplasm, Dylan Larkin

Graduate Theses and Dissertations

Genomic selection (GS) is an important tool for increasing genetic gain for economically important traits in breeding programs. Genomic selection uses molecular markers across the entire genome in order to predict the performance of breeding lines for a trait of interest prior to phenotyping. A training population (TP) of elite germplasm, representative of the University of Arkansas wheat breeding program, was developed in order to predict important agronomic and Fusarium head blight (FHB) resistance traits within the University of Arkansas wheat breeding program through cross-validation and forward prediction.

A genome-wide association study (GWAS) was performed on the TP to identify …

Genetic Diversity: The Hub Of Plant Breeding, Foster Kangben

International Programs

Agriculture the world over is faced with the threat of food, fiber, and nutrition insecurity especially in the wake of climate change. However, employing two approaches to plant breeding, the molecular and phenotypic methods, can make use of genetic diversity in crop species in order to help surmount these challenges.

Identification Of Quantitative Trait Loci (Qtl) For Tolerance To Metribuzin In Soft Red Winter Wheat (Triticum Aestivum L.), Hugo Alfonso Anzueto Ponciano

LSU Master's Theses

Wheat is one of the world’s most important food crops and provides nearly 20% of the calories consumed. Wheat is the third most important crop after corn and rice on a global production scale. In Louisiana wheat harvest was about 6000 hectares in 2017. Wheat production is negatively affected by several biotic and abiotic factors including weather, diseases, and weeds. Metribuzin is a broad-spectrum herbicide commonly used in wheat because it is inexpensive and controls most of the common weeds in wheat fields. Metribuzin has benefits for the grower controlling weeds that might cause yield loss in varieties or lines …

Efficiency Of The Nuclease I-Scei In Excising Selectable Marker Genes From The Plant Genome, Elliot E. Pruett, Soumen Nandy, Vibha Srivastava

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Gene stacking is a method used in biotechnology by which multiple genes can be placed at a single genomic site, thereby simplifying plant breeding. In this approach, DNA nucleases are used for excising selectable marker genes (SMG), which are the unneeded components of transgenic plants. The goal of this project is to evaluate the effectiveness of the nuclease I-SceI in excising DNA in plants. Specifically, this study tests heat-inducible I-SceI through the use of a heat-shock promoter (HS) in order to control SMG excision by heat application. The DNA plasmid containing a visual marker gene flanked by I-SceI target sites …

Applying Molecular And Phenotypic Tools To Characterize Flesh Texture And Acidity Traits In The Arkansas Peach Breeding Program And Understanding The Crispy Texture In The Arkansas Blackberry Breeding Program, Alejandra Andrea Salgado Rojas

Graduate Theses and Dissertations

Quantitative trait loci (QTL) utilizing a pedigree-based analysis (PBA) approach was performed in the peach breeding program for the first time. The pedigree consisted of seven-F1 populations, their parents, ancestors, and cultivars. Flesh firmness and titratable acidity (TA) were examined in 2011, 2012, and 2013. For TA, a consistent and strong QTL was identified on the proximal end of linkage group (LG) 5 of the peach genome. For flesh firmness, two QTLs were located on LG 4. The first QTL was located on the chromosomal region where the slow-melting flesh (SMF) DNA test was identified, and the second QTL was …

Anaerobic Detoxification Of Acetic Acid In A Thermophilic Ethanologen, A Joe Shaw, Bethany B. Miller, Stephen R. Rogers, William Robert Kenealy, Alex Meola, Ashwini Bhandiwad, W Ryan Sillers, Indraneel Shikhare, David Hogsett, Christopher Herring

Dartmouth Scholarship

The liberation of acetate from hemicellulose negatively impacts fermentations of cellulosic biomass, limiting the concentrations of substrate that can be effectively processed. Solvent-producing bacteria have the capacity to convert acetate to the less toxic product acetone, but to the best of our knowledge, this trait has not been transferred to an organism that produces ethanol at high yield. We have engineered a five-step metabolic pathway to convert acetic acid to acetone in the thermophilic anaerobe Thermoanaerobacterium saccharolyticum.

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Tracking The Cellulolytic Activity Of Clostridium Thermocellum Biofilms, Alexandru Dumitrache, Gideon M. Wolfaardt, David Allen, Steven N. Liss, Lee R. Lynd

Dartmouth Scholarship

Microbial cellulose conversion by Clostridium thermocellum 27405 occurs predominantly through the activity of substrate-adherent bacteria organized in thin, primarily single cell-layered biofilms. The importance of cellulosic surface exposure to microbial hydrolysis has received little attention despite its implied impact on conversion kinetics.

Aronia Mitschurinii: Solving A Horticultural Enigma, Peter J. Leonard

Master's Theses

Aronia (Medik.), commonly known as chokeberry, is a taxonomically misunderstood genus currently experiencing a renaissance in North America as both an ornamental and fruit crop. Three species of chokeberry are commonly accepted as native in North America: A. arbutifolia (L.) Pers. red chokeberry; A. melanocarpa (Michx.) Elliot, black chokeberry; and A. prunifolia (Marshall) Rehder, or purple chokeberry. In Europe a fourth species of human origin is recognized as Aronia mitschurinii (A.K.Skvortsov & Maitul.), or cultivated, black-fruited Aronia. It is widely speculated that this genotype originated in the early 20^th century with Russian pomologist Ivan Michurin, as the product …

Genetically Modified Canola Trials In 2009, Department Of Agriculture And Food, Western Australia

Research Reports

During 2009, just over 860 hectares of Roundup Ready® canola were grown in Western Australia enabled by an exemption order under the Genetically Modified Crops Free Areas Act 2003. The aim of the trials was to establish whether GM canola could be segregated from non-GM canola along the WA supply chain, and whether GM canola was agronomically viable for WA farming systems. This was the first time genetically modified canola had been grown on a commercial scale in WA - earlier exemptions for smaller trials had been for variety testing and scientific purposes.

Crop Updates 2003 - Geraldton, David Stephens, James Fisher, Ian Edwards, Dennis Wise, Andrew Young, Rob Loughman, Ciara Beard, Greg Shea, Blakely Paynter, Roslyn J. Jettner, Kevin Young, Kevin Walden, Geoff Thomas, Robin Wilson, Iain Barclay, Robyn Mclean, Jenny Garlinge, Bill Lambe, Neil Venn, Peter Clarke, Martin Harries, Bob French, Wayne Parker, Murray Blyth, Peter Norris, Greg Lyle, Katheryn Steadman, Amanda Ellery, Sally C. Peltzer, Kari-Lee R. Falconer, Russell Speed, Terry Piper, Harmohinder Dhammu, Bindi Webb, Paul Blackwell, Phil Logue, Nigel Moffat, Rohan Ford, Miles Obst, Frances Hoyle, Leanne Schulz, Judith Devenish, Peter Newman, Grant Morrow, Ian Foster, Darshan Sharma, Marnie Thomas, Graham Walton, Hasan Zaheer, Paul Carmody, Mike Clarke, Gregory Paul Raper

Crop Updates

This session covers twenty eight papers from different authors

1. Seasonal Outlook: What is in store for 2003, David Stephens, Department of Agriculture

2. Examining The Management Options For Wheat Crops In The Coming Season, James Fisher, Department of Agriculture

3. GMO’s – what do they offer? Ian Edwards, Grain Bio Tech Australia Pty Ltd

4. The Big Gamble – Wheat prices for 2003, Dennis Wise, Profarmer

5. Market outlook for other grains, Andrew Young, General Manager Agricorp

6. Stripe rust – where to now for the WA wheat industry? Robert Loughman, Ciara Beard and Greg Shea, Department of Agriculture

7. Baudin …

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Crop Updates 2003 - Cereals, Graham Crosbie, Robert Loughman, Collin Wellings, Greg Shea, Simon Mckirdy, Neil C. Turner, Brenda Shackley, Wal Anderson, Darshan Sharma, Mohammad Amjad, Steve Penny Jr, Melanie Kupsch, Anne Smith, Veronika Reck, Pam Burgess, Glenda Smith, Elizabeth Tierney, Peter Burges, Moin U. Salam, Megan Collins, Art Diggle, Blakely Paynter, Roslyn J. Jettner, Kevin Young, Jocelyn Ball, Natasha Littlewood, Lucy Anderton, Irene Waters, Tim Setter, Jeff Russell, Reg Lance, Chengdao Li, Sue Broughton, Michael Jones, Grace Zawko, Keith Gregg, Stephen Loss, Frank Ripper, Ryan Guthrie, Daniel Bell, Patrick Gethin, Narelle Hill, Laurence Caeslake, Vivien Vanstone, Sean Kelly, Helen Hunter, Christopher R. Newman

Crop Updates

This session covers twenty one papers from different authors:

PLENARY

1. Recognising and responding to new market opportunities in the grains industry, Graham Crosbie, Manager, Grain Products Research, Crop Breeding, Plant Industries, Department of Agriculture

2. Stripe rust – where to now for the WA wheat industry? Robert Loughman¹, Colin Wellings² and Greg Shea¹¹Department of Agriculture, ²University of Sydney Plant Breeding Institute, Cobbitty (on secondment from NSW Agriculture)

3. Benefits of a Grains Biosecurity Plan, Dr Simon McKirdy, Plant Health Australia, Mr Greg Shea, Department of Agriculture

4. Can we improve …

Proceedings Of Carrot Conference Australia, Perth, Western Australia, Figaro Natoli, Sam Calameri, Albert Grubelich, Allan Mckay, Elaine Davison, David Ellement

Conference papers and presentations

No abstract provided.

Accelerating Variety Release With Double Haploids, Sue Broughton

Journal of the Department of Agriculture, Western Australia, Series 4

The use of plant tissue culture to produce special lines called doubled haploids is reducing the time taken to breed new varieties of cereal crops by up to three years. Sue Broughton outlines what doubled haploids are, how they are produced, and why they have been able to short circuit the usual lengthy breeding process.

Genetic Markers In Plant Evolutionary Ecology, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Genetic markers have provided plant ecologists with a method of assessing levels of genetic relatedness among individuals and populations. In recent years a number of techniques based on DNA sequence variation have been developed to complement allozyme methods that are already widely used. Some of these new markers are more variable than protein-based markers, allowing more precise estimates of genetic differences among individuals and populations. Other DNA-based markers are based on organelle genomes that are inherited uniparentally. These cytoplasmic markers can provide a method for assessing the separate effects of seed and pollen dispersal on gene flow within and among …

Postpollination Mechanisms Influencing Mating Patterns And Fecundity: An Example From Eichhornia Paniculata, Mitchell B. Cruzan

Biology Faculty Publications and Presentations

Plant mating systems are influenced by the amount and genetic composition of pollen grains deposited on stigmas and by the ability of recipients to discriminate among pollen from different sources. We describe an experimental procedure that uses limiting and excess pollinations with mixtures of genetically marked pollen to partition the siring success of donors into three components: prefertilization gamete attrition (failure of male gametophytes before fertilization), pollen competitive ability (differences in pollen tube growth rate), and postfertilization gamete attrition (embryo abortion). Regression models for the relationships of pollen load size with each pollen's siring success and total recipient fecundity indicate …

Planting Seeds For A Sound Future, Peter Portmann

Journal of the Department of Agriculture, Western Australia, Series 4

Royalties have been collected sales of all new crop varieties released in We tern ustralia by the Department of Agriculture since the lease of Merrit lupins and Yilgarn oats in 1991.

Many questions have been raised by farmers about these royalties. Why pay royalties when we have already paid for the development of the varieties through our Grains Research end: Development Corporation levy? Why should we pay a Jevy if it - is just going back into Government revenue? Is there any real benefit to us?

Biotechnology : Exposing The Myths & Realities, Sue Sutherland, Alan Lymbery

Journal of the Department of Agriculture, Western Australia, Series 4

Biotechnology has become one of the buzz words of the 1990s. Sounds impressive but what's it all about? Sue Sutherland and Alan Lymbery unravel some of the jargon and explore its potential for Western Australian agriculture.

Developing Improved Varieties Of Subterranean Clover, Phil Nichols, Bill Collins, Dennis Gillespie, Martin Barbetti

Journal of the Department of Agriculture, Western Australia, Series 4

Subterranean clover is widely recognised as the most important pasture legume species in southern Australia, having been sown over about 20 million hectares.

Together with superphosphate and trace elements, it has been a key factor in improving and maintaining soil fertility, and has resulted in dramatic increases in crop, livestock and wool production.

While the value of subterranean clover is well established, it does have some shortcomings.

Many varieties grown today are not sufficiently adapted to the environments and management systems in use. Others lack sufficient pest and disease resistance.

Improved subterranean clovers are being bred with better adaptation and …

Fast Tracking Barley Varieties Using Anther Culture, Sue Broughton, Penny Priest

Journal of the Department of Agriculture, Western Australia, Series 4

Breeding new varieties of barley or other cereal crops usually takes between 12 and 15 years. five years of that time may be needed to stabalise the new varieties so that they breed trueto type, but anther culture can reduce this delay to only eight months. This technology will allow the Department of Agriculture's barley breeding programme to respond more rapidly to changes in goals set by industry and to meet market demands.

A Tale Of Two Ladies : Pink Lady And Sundowner, John Cripps, Eleanor Melvin-Carter

Journal of the Department of Agriculture, Western Australia, Series 4

It is five years since the first Western Australianbred Pink Lady and Sundowner apples were sold on the local market. In that time, these crisp, sweet flavoursome apples have become top-selling, premium fruit.

The story is no different overseas. Trial shipments to London, Taiwan and Singapore have indicated a big demand for the unique Pink Lady apple - far more than Western Australia can presently supply.

One million fruiting Pink Lady apple trees are needed to meet anticipated export sales. Today, there are only 100,000 trees in the ground, of which about half are bearing fruit.

New high quality apple …

Domestication Of Rough-Seeded Lupins, Bevan Buirchell, Wallace Cowling

Journal of the Department of Agriculture, Western Australia, Series 4

Since the beginning of civilisation, humans have been domesticating wild plants such as wheat, barley and rice. They have both conciously and unconciously selected plant types suited to cropping.

Reccently, there has been an increasing effort to domesticate a greater variety of wild plants, especially legumes.

Breeding Field Peas : Screening Pea Lines, G H. Walton

Journal of the Department of Agriculture, Western Australia, Series 4

The early use of field peas in Western Australia was as a green manure crop or to feed stock be grazing the mature crop. Varieties which produced a large bulk of green material were sown. There was no control over insects or diseases.

In 1985, the Feseral Government recognised that to realize the potential of grain legumes grown throughout Australia, more research was needed.

One of the Grain Legume Research Council's (GLRC) priorities is species evaluation and variety improvement.

Since 1983, the Western Australian Department of Agriculture has screened advanced crossbred lines from the South Australian breeding programme for its …

Breeding Field Peas In Western Australia, T N. Khan

Journal of the Department of Agriculture, Western Australia, Series 4

Field peas have grown dramatically in popularity in western Australia in the past five years, with the planted area increasing from a mere few hundred hectares to about 70,000 ha in 1988. This growth may continue, as more than 700,000 ha of agricultural land is potentially suitable for growing field peas.

However, any such growth of the pea industry will require new cultivars with improved yield, adaptation and quality characteristics to meet local and export demands. The Department of agriculture therefore decided to start a selection and breeding programme to support the pea industry.

Increasing Protein Content Of Wheat By Breeding, B R. Whan, Graham Crosbie

Journal of the Department of Agriculture, Western Australia, Series 4

The most efficient means of increasing protein content of WesternAustralian wheat is by breeding new varieties with higher inherent protein in their grain. But is this possible without sacrificing yeild or other desirable characteristics?

Phomopsis-Resistanct Lupins : Breakthrough Towards The Control Of Lupinosis, W A. Cowling, J. G. Allen, P Mcr. Wood, J. Hamblin

Journal of the Department of Agriculture, Western Australia, Series 4

Attemots to control lupinosis in sheep have been thwarted by the unpredictable occurence of the disease in the field. The complex interaction of the toxin-producing fungus (Phomopsis leptostromiformis) with its host (the lupin plant), together with variable weather and paddock grazing conditions, have made it difficult to predict when stock are in danger of contracting the disease. The risk of lupinosis discourages many farmers from growing lupins, despite the many potential benefits of including them in crop rotations.

A team of Department of Agriculture plant breeders, plant pathologists, and animal scientists set out in the mid 1970s to find resistance …

Preventing Lupinosis With Phomopsis-Resistant Lupins, Jeremy G. Allen, W. A. Cowling

Journal of the Department of Agriculture, Western Australia, Series 4

Lupinosis is one of the major livestock siseases in Western Australia. It is caused by stock eating toxins produced by the fungus Phomopsis leptostromiformis which colonises the stems of dead lupin plants.

In 975, the Department of Agriculture started a breeding programme to develop Phompsis-resistant lupins to overcome or control lupinosis. In the previous issue of this Journal the results of small plot evaluations of new lines of Phompsis-resistant lupins developed in this programme were reported. This article describes the first trial involving grazing of Phompsis-resistant lupins.