Plant Breeding and Genetics Commons^™

Squash Variety Trials Summer 2012, Rebecca Brown

University of Rhode Island Vegetable Production Research Reports

Variety trials of summer squashes and zucchini conducted in Kingston, RI, USA.

Leek Variety Trials, 2012, Rebecca Brown, Noah Leclaire-Conway

University of Rhode Island Vegetable Production Research Reports

Variety trial of leek varieties conducted at Kingston, RI, in 2012.

Melon Variety Trials, 2012, Rebecca Brown

University of Rhode Island Vegetable Production Research Reports

Melon variety trial conducted at Kingston, RI, in summer of 2012.

Onion Variety Trials, 2012, Rebecca Brown, Noah Leclaire-Conway

University of Rhode Island Vegetable Production Research Reports

No abstract provided.

Cucumber Variety Trials 2012, Rebecca Brown

University of Rhode Island Vegetable Production Research Reports

Slicing and pickling cucumber variety trials conducted at Kingston, RI, during the summer of 2012.

Pseudomonas Hopu1 Modulates Plant Immune Receptor Levels By Blocking The Interaction Of Their Mrnas With Grp7, Valerie Nicaise, Anna Joe, Byeong-Ryool Jeong, Christin Korneli, Freddy Boutrot, Isa Westedt, Dorothee Staiger, James R. Alfano, Cyril Zipfel

Center for Plant Science Innovation: Faculty Publications

Pathogens target important components of host immunity to cause disease. The Pseudomonas syringae type III-secreted effector HopU1 is a mono-ADP-ribosyltransferase required for full virulence on Arabidopsis thaliana. HopU1 targets several RNA-binding proteins including GRP7, whose role in immunity is still unclear. Here, we show that GRP7 associates with translational components, as well as with the pattern recognition receptors FLS2 and EFR. Moreover, GRP7 binds specifically FLS2 and EFR transcripts in vivo through its RNA recognition motif. HopU1 does not affect the protein–protein associations between GRP7, FLS2 and translational components. Instead, HopU1 blocks the interaction between GRP7 and FLS2 and …

Small Interfering Rna–Mediated Translation Repression Alters Ribosome Sensitivity To Inhibition By Cycloheximide In Chlamydomonas Reinhardtii, Xinrong Ma, Eun-Jeong Kim, Insun Kook, Fangrui Ma, Adam Voshall, Etsuko N. Moriyama, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty Publications

Small RNAs (sRNAs; approximately 20 to 30 nucleotides in length) play important roles in gene regulation as well as in defense responses against transposons and viruses in eukaryotes. Their biogenesis and modes of action have attracted great attention in recent years. However, many aspects of sRNA function, such as the mechanism(s) of translation repression at postinitiation steps, remain poorly characterized. In the unicellular green alga Chlamydomonas reinhardtii, sRNAs derived from genomeintegrated inverted repeat transgenes, perfectly complementary to the 39 untranslated region of a target transcript, can inhibit protein synthesis without or with only minimal mRNA destabilization. Here, we report …

Complementarity To An Mirna Seed Region Is Sufficient To Induce Moderate Repression Of A Target Transcript In The Unicellular Green Alga Chlamydomonas Reinhardtii, Tomohito Yamasaki, Adam Voshall, Eun-Jeong Kim, Etsuko N. Moriyama, Heriberto Cerutti, Takeshi Ohama

Center for Plant Science Innovation: Faculty Publications

MicroRNAs (miRNAs) are 20–24 nt noncoding RNAs that play important regulatory roles in a broad range of eukaryotes by pairing with mRNAs to direct post-transcriptional repression. The mechanistic details of miRNA-mediated post-transcriptional regulation have been well documented in multicellular model organisms. However, this process remains poorly studied in algae such as Chlamydomonas reinhardtii, and specific features of miRNA biogenesis, target mRNA recognition and subsequent silencing are not well understood. In this study, we report on the characterization of a Chlamydomonas miRNA, cre-miR1174.2, , which is processed from a near-perfect hairpin RNA. Using Gaussia luciferase (gluc) reporter genes, …

Camelina Seed Transcriptome: A Tool For Meal And Oil Improvement And Translational Research, Huu T. Nguyen, Jillian E. Silva, Ram Podicheti, Jason Macrander, Wenyu Yang, Tara J. Nazarenus, Jeong-Won Nam, Jan G. Jaworski, Chaofu Lu, Brian E. Scheffler, Keithanne Mockaitis, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Camelina (Camelina sativa), a Brassicaceae oilseed, has received recent interest as a biofuel crop and production platform for industrial oils. Limiting wider production of camelina for these uses is the need to improve the quality and content of the seed protein-rich meal and oil, which is enriched in oxidatively unstable polyunsaturated fatty acids that are deleterious for biodiesel. To identify candidate genes for meal and oil quality improvement, a transcriptome reference was built from 2047 Sanger ESTs and more than 2 million 454-derived sequence reads, representing genes expressed in developing camelina seeds. The transcriptome of approximately 60K transcripts …

Comparative Analyses Of Two Geraniaceae Transcriptomes Using Next-Generation Sequencing, Jinxin Zhang, Tracey A. Ruhlman, Jeffrey P. Mower, Robert K. Jansen

Center for Plant Science Innovation: Faculty Publications

Background: Organelle genomes of Geraniaceae exhibit several unusual evolutionary phenomena compared to other angiosperm families including accelerated nucleotide substitution rates, widespread gene loss, reduced RNA editing, and extensive genomic rearrangements. Since most organelle-encoded proteins function in multi-subunit complexes that also contain nuclear-encoded proteins, it is likely that the atypical organellar phenomena affect the evolution of nuclear genes encoding organellar proteins. To begin to unravel the complex co-evolutionary interplay between organellar and nuclear genomes in this family, we sequenced nuclear transcriptomes of two species, Geranium maderense and Pelargonium x hortorum.

Results: Normalized cDNA libraries of G. maderense and …

Arabidopsis 56–Amino Acid Serine Palmitoyltransferase- Interacting Proteins Stimulate Sphingolipid Synthesis, Are Essential, And Affect Mycotoxin Sensitivity, Athen N. Kimberlin, Saurav Majumder, Gongshe Han, Ming Chen, Rebecca E. Cahoon, Julie M. Stone, Teresa M. Dunn, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Maintenance of sphingolipid homeostasis is critical for cell growth and programmed cell death (PCD). Serine palmitoyltransferase (SPT), composed of LCB₁ and LCB₂ subunits, catalyzes the primary regulatory point for sphingolipid synthesis. Small subunits of SPT (ssSPT) that strongly stimulate SPT activity have been identified in mammals, but the role of ssSPT in eukaryotic cells is unclear. Candidate Arabidopsis thaliana ssSPTs, ssSPTa and ssSPTb, were identified and characterized. Expression of these 56–amino acid polypeptides in a Saccharomyces cerevisiae SPT null mutant stimulated SPT activity from the Arabidopsis LCB₁/LCB₂ heterodimer by >100-fold through physical interaction with LCB …

Pseudomonas Syringae Pv. Syringae Uses Proteasome Inhibitor Syringolin A To Colonize From Wound Infection Sites, Johana C. Misas-Villamil, Izabella Kolodziejek, Emerson Crabill, Farnusch Kaschani, Sherry Niessen, Takayuki Shindo, Markus Kaiser, James R. Alfano, Renier A. L. Van De Hoorn

Center for Plant Science Innovation: Faculty Publications

Infection of plants by bacterial leaf pathogens at wound sites is common in nature. Plants defend wound sites to prevent pathogen invasion, but several pathogens can overcome spatial restriction and enter leaf tissues. The molecular mechanisms used by pathogens to suppress containment at wound infection sites are poorly understood. Here, we studied Pseudomonas syringae strains causing brown spot on bean and blossom blight on pear. These strains exist as epiphytes that can cause disease upon wounding caused by hail, sand storms and frost. We demonstrate that these strains overcome spatial restriction at wound sites by producing syringolin A (SylA), a …

Functional Modeling Identifies Paralogous Solanesyl-Diphosphate Synthases That Assemble The Side Chain Of Plastoquinone-9 In Plastids, Anna Block, Rikard Fristedt, Sara Rogers, Jyothi Kumar, Brian Barnes, Joshua Barnes, Christian Elowsky, Yashitola Wamboldt, Sally Ann Mackenzie, Kevin Redding, Sabeeha S. Merchant, Gilles J. Basset

Center for Plant Science Innovation: Faculty Publications

Background: Plastid isoforms of solanesyl-diphosphate synthase catalyze the elongation of the prenyl side chain of plastoquinone-9.

Results: Corresponding mutants display lower levels of plastoquinone-9 and plastochromanol-8 and display intact levels of vitamin E.

Conclusion: Plastochromanol-8 originates from a subfraction of non-photoactive plastoquinol-9 and is not essential for seed longevity.

Significance: Viable plastoquinone-9 mutants are invaluable tools for understanding plastid metabolism.

Mutational Analyses Of A Fork Head Associated Domain Protein, Dawdle, In Arabidopsis Thaliana, Lakshmi Ayiloor Narayanan, Dipaloke Mukherjee, Shuxin Zhang, Bin Yu, David Chevalier

Center for Plant Science Innovation: Faculty Publications

DAWDLE (DDL) gene encodes a protein that contains an N-terminal arginine-rich domain and a C-terminal Fork Head Associated (FHA) domain in Arabidopsis thaliana. DDL protein is believed to function in microRNA biogenesis by mediating the recruitment of pri-microRNA to DICER-LIKE 1 and also stabilizing the microRNA. The aim of this study was to conduct a structure-function analysis to identify the regions in DDL that are of functional significance. Targeted Induced Local Lesions in Genome screen was performed in the Columbia erecta-105 background of Arabidopsis resulting in the identification of eight point mutations spanning DDL. The mutants were characterized by …

Cdc5, A Dna Binding Protein, Positively Regulates Posttranscriptional Processing And/Or Transcription Of Primary Microrna Transcripts, Shuxin Zhang, Meng Xie, Guodong Ren, Bin Yu

Center for Plant Science Innovation: Faculty Publications

CDC5 is a MYB-related protein that exists in plants, animals, and fungi. In Arabidopsis, CDC5 regulates both growth and immunity through unknown mechanisms. Here, we show that CDC5 from Arabidopsis positively regulates the accumulation of microRNAs (miRNAs), which control many biological processes including development and adaptations to environments in plants. CDC5 interacts with both the promoters of genes encoding miRNAs (MIR) and the DNA-dependent RNA polymerase II. As a consequence, lack of CDC5 reduces the occupancy of polymerase II at MIR promoters, as well as MIR promoter activities. In addition, CDC5 is associated with the DICER–LIKE1 complex, …

Comparative Studies Of Differential Gene Calling Using Rna-Seq Data, Ximeng Zheng, Etsuko N. Moriyama

Center for Plant Science Innovation: Faculty Publications

Background: With its massive amount of data, gene-expression profiling by RNA-Seq has many advantanges compared with microarray experiments. RNA-Seq analysis, however, is fundamentally different from microarray data analysis. Techniques developed for analyzing microarray data thus cannot be directly applicable for the digital gene expression data. Several statistical methods have been developed for identifying differentially expressed genes specifically from RNA-Seq data over the past few years.
Results: In this study, we examined the performance of differential gene-calling methods using RNA-Seq data in practical situations. We focused on two representative methods: one parametric method, DESeq, and one nonparametric method, NOISeq. …

A Novel Function Prediction Approach Using Protein Overlap Networks, Shide Liang, Dandan Zheng, Daron M. Standley, Huarong Guo, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Construction of a reliable network remains the bottleneck for network-based protein function prediction. We built an artificial network model called protein overlap network (PON) for the entire genome of yeast, fly, worm, and human, respectively. Each node of the network represents a protein, and two proteins are connected if they share a domain according to InterPro database.
Results: The function of a protein can be predicted by counting the occurrence frequency of GO (gene ontology) terms associated with domains of direct neighbors. The average success rate and coverage were 34.3% and 43.9%, respectively, for the test genomes, …

A Thraustochytrid Diacylglycerol Acyltransferase 2 With Broad Substrate Specificity Strongly Increases Oleic Acid Content In Engineered Arabidopsis Thaliana Seeds, Chunyu Zhang, Umidjon Iskandarov, Elliott T. Klotz, Robyn L. Stevens, Rebecca E. Cahoon, Tara J. Nazarenus, Suzette L. Pereira, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Diacylglycerol acyltransferase (DGAT) catalyses the last step in acyl-CoA-dependent triacylglycerol (TAG) biosynthesis and is an important determinant of cellular oil content and quality. In this study, a gene, designated TaDGAT2, encoding a type 2 DGAT (DGAT2)-related enzyme was identified from the oleaginous marine protist Thraustochytrium aureum. The deduced TaDGAT2 sequence contains a ~460 amino acid domain most closely related to DGAT2s from Dictyostelium sp. (45–50% identity). Recombinant TaDGAT2 restored TAG biosynthesis to the Saccharomyces cerevisiae H1246 TAG-deficient mutant, and microsomes from the complemented mutant displayed DGAT activity with C16 and C18 saturated and unsaturated fatty acyl-CoA and diacylglycerol …

Identification Of Sources Of Resistance To Damping-Off And Early Root/Hypocotyl Damage From Rhizoctonia Solani In Common Bean (Phaseolus Vulgaris L.), P. A. Peña, James R, Steadman, Kent M. Eskridge, Carlos A. Urrea

Panhandle Research and Extension Center

Rhizoctonia solani causes economically important root and hypocotyl diseases in common bean throughout the world. Root health is a vital factor in plant development and root diseases would negatively influence water and nutrient uptake as well as cause direct stand reduction and root rot damage to the crop. An efficient common bean screening method to evaluate damping-off and early root/ hypocotyl damage from R. solani was developed and used to identify dry bean lines with levels of resistance to this disease. Two sets of 163 and 111 lines previously evaluated for drought tolerance in Nebraska and Puerto Rico were evaluated …

Fuzzy Clustering Of Cpp Family In Plants With Evolution And Interaction Analyses, Tao Lu, Yongchao Dou, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Transcription factors have been studied intensively because they play an important role in gene expression regulation. However, the transcription factors in the CPP family (cystein-rich polycomb-like protein), compared with other transcription factor families, have not received sufficient attention, despite their wide prevalence in a broad spectrum of species, from plants to animals. The total number of known CPP transcription factors in plants is 111 from 16 plants, but only 2 of them have been studied so far, namely TSO1 and CPP1 in Arabidopsis thaliana and soybean, respectively.
Methods: In this work, to study their functions, we applied …

Germinación In Vitro De Laelia Speciosa (Kunth) Schltr., Una Herramienta Para Su Conservación Ex Situ, María Ascención Aguilar-Morales, Ana Laura López-Escamilla

Estudios científicos en el estado de Hidalgo y zonas aledañas

Dentro de la familia Orchideaceae, Laelia speciosa (Kunth) Schltr. es probablemente la orquídea más explotada en México, por lo que es importante establecer estrategias de conservación. El objetivo del presente trabajo fue determinar el mejor medio de cultivo para la germinación in vitro de semillas. Cápsulas maduras e inmaduras de L. speciosa, fueron colectadas en la Reserva de la Biosfera Barranca de Metztitlán, Hidalgo, México y las semillas se sembraron en condiciones in vitro en cuatro tratamientos: Murashige y Skoog (MS) (1962) al 100%; MS al 50%; Knudson C (KC) (1946) al 50% y KC al 100%. A los …

Nutrient Contents, Color, Texture, And Sensory Evaluation Of 12 Arkansas Grown Soybean Cultivars In Canned Products, Quyen Nguyen, Navam Hettiarachchy, Srinivas J. Rayaprolu

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Soybean was introduced in the U.S. in the 1800s, and it has been proven to have several health benefits. New cultivars of soybeans with varying hull colors have been developed using plantbreeding technology. Canning is one of the effective processing methods to extend the shelf life of products. However, very little information is available on canned soybeans. This research studied the composition of 12 soybean cultivars including two cultivars with brown seed coat (R08-4014 and R09-349), three cultivars with black seed coat (R07-1927, R07-10396, and R09-345), and seven regular cultivars with yellow seed coat (R05-1772, R05-4969, R07-2001, R08-4005, R08- 4006, …

Evaluation Of Cover Crops In High Tunnel Vegetable Rotation, Tyler A. Patrick, Neal Mays, Jason Mcafee, Curt R. Rom

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Organic vegetable production within high tunnels allows for an extended growing season, crop protection, and environmental control. The USDA National Organic Program (NOP) standards mandate evidence that the soil has been maintained and improved over the course of production. Previous studies have indicated the potential of cover crops for reducing competitive vegetation, and improving soil quality, thus resulting in greater plant growth, nutrient uptake, and yield. However, there has been limited work in the confines of high tunnels as part of a tunnel-system rotation. Ten nitrogen-fixing and ten non-legume cover crops were established under a high tunnel and evaluated for …

Characterization Of Seediness Attributes Of Blackberry Genotypes, Bethany Sebesta, John R. Clark, Renee T. Threlfall, Luke R. Howard

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Fresh market blackberries can feel “seedy” when consumed. This “seediness” is associated with the presence of pyrenes which are comprised of a single seed enclosed in an endocarp. Small pyrene size  (<3 mg) is preferred in both fresh-market and processed blackberry products. Yet, the proportion of pyrene weight to total berry weight can be more important than pyrene size. The objective of this study was to determine and compare descriptive sensory analysis and pyrene characteristics of blackberry genotypes from the University of Arkansas System Division of Agriculture blackberry breeding program. Panelists were trained according to Spectrum® methods and evaluated 20 genotypes for overall seediness. Pyrene characteristics, including weight and dimension, were measured for 22 genotypes. Seven of the 22 genotypes had an individual pyrene weight of 3 mg or less. Pyrene weight to berry weight ratio ranged from 2.7% (‘Tupy’) to 5.4% (‘Prime-Ark® 45’). ‘Tupy’ had low individual pyrene weights and a low ratio, which are most likely factors that contribute to its widespread acceptance by consumers. Pyrene weight to berry weight ratio was positively correlated to descriptive overall seediness (r = 0.70) but not to number of pyrenes/berry. Therefore, finding a desirable pyrene weight to berry weight ratio is integral to decreasing perceived seediness in the development of new blackberry cultivars.

A Study Of The Genes Tfl1 And Lfy As Agents In The Divergent Evolution Of The Plant Species Arabidopsis Thaliana And Carica Papaya, Ryan Lockard

Honors Program Theses

The long term goals of this study are to: (1) To determine if the genes TFLI and LFY perform any role in the divergent evolution and development of these two plant species; (2) If so, begin to assess whether the homologs' functional divergence is a result of gene regulation, protein function, both, or some different genetic mechanism altogether. These goals will be accomplished by genetically transforming Arabidopsis thaliana plants containing TFLI and LFY knockout mutations (those genes removed) with the homologous genes from Carica papaya in order to compare differences in development between the plants resulting from the influence of …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2012, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

Indiana growers harvested sweet corn for fresh market sales from 5,800 acres in 2011, with an average yield of 67 cwt/acre (159 crates or 3.3 tons per acre) and a total value of $17.9 million (USDA NASS, 2012). Indiana ranked 15th among states for production of fresh market sweet corn and produced about 1.3% of the nation’s total in 2011. The 2007 USDA Ag Census reported 603 Indiana farms producing sweet corn for fresh markets and 51 farms selling to processors. Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most …

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2012, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

Indiana growers harvested sweet corn for fresh market sales from 5,800 acres in 2011, with an average yield of 67 cwt/acre (159 crates or 3.3 tons per acre) and a total value of $17.9 million (USDA NASS, 2012). Indiana ranked 15th among states for production of fresh market sweet corn and produced about 1.3% of the nation’s total in 2011. The 2007 USDA Ag Census reported 603 Indiana farms producing sweet corn for fresh markets and 51 farms selling to processors. Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most …

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2012, Elizabeth Maynard

Midwest Vegetable Trial Reports

Indiana growers harvested sweet corn for fresh market sales from 5,800 acres in 2011, with an average yield of 67 cwt/acre (159 crates or 3.3 tons per acre) and a total value of $17.9 million (USDA NASS, 2012). Indiana ranked 15th among states for production of fresh market sweet corn and produced about 1.3% of the nation’s total in 2011. The 2007 USDA Ag Census reported 603 Indiana farms producing sweet corn for fresh markets and 51 farms selling to processors. Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2012, Elizabeth Maynard

Midwest Vegetable Trial Reports

Indiana growers harvested sweet corn for fresh market sales from 5,800 acres in 2011, with an average yield of 67 cwt/acre (159 crates or 3.3 tons per acre) and a total value of $17.9 million (USDA NASS, 2012). Indiana ranked 15th among states for production of fresh market sweet corn and produced about 1.3% of the nation’s total in 2011. The 2007 USDA Ag Census reported 603 Indiana farms producing sweet corn for fresh markets and 51 farms selling to processors. Sweet corn fields for fresh market sales are located throughout the state. In northern Indiana, bicolor corn is most …

Sorghum Transformation: Overview And Utility, Tejinder Kumar, Arlene Howe, Shirley Sato, Ismail M. Dweikat, Tom E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Over the past decade genomics resources available for sorghum have rapidly expanded (Paterson Int J Plant Genomics 2008:6, 2008), these resources, coupled with the recent completion of the genome sequence which is relatively small in size (730 Mb) (Paterson et al. Nature 457:551–556, 2009) makes sorghum a rather attractive species to study. Moreover, the USDA germplasm system maintains 42,614 accessions, of which more than 800 exotic landraces have been converted to day length-insensitive lines to facilitate their use in breeding programs. In addition, a set of EMS mutation stocks developed by the USDA Plant Stress and Germplasm Development Unit in …