Plant Breeding and Genetics Commons^™

Morphogenesis And Tissue Culture Studies Of Gerbera Jamesonii Bolus Ex. Hook F., Nor Azlina Hasbullah

Student Works (2010-2019)

In vitro regeneration of Gerbera jamesonii Bolus ex. Hook f. was successfully carried out in this study. Petiole and leaf explants from 8-week-old aseptic seedlings were used as source of explants. Different response was observed when different explants were cultured on the culture media. MS (Murashige and Skoog, 1962) supplemented with 2.0 mg/l BAP and 0.5 mg/l NAA was identified as the optimum medium for shoot regeneration, while petiole explant was identified as the most responsive explant in this study. Petiole explants regenerated shoots (94.3 } 2.5 %) and produced 9.3 } 0.6 shoots per explant. MS basal medium was …

Molecular Characterization And Expression Analysis Of Putative Rpk1 And Putative Clv1 In Malaysian Indica Rice (Oryza Sativa L.) Cv.Mr219 And Malaysian Wild Rice (Oryza Rufipogon) Irgc105491., Law Yee Song

Student Works (2010-2019)

Two candidate yield-related genes, putative Receptor-like Protein Kinase 1 (putative RPK1) and putative CLAVATA1 Receptor Kinase (putative CLV1), were selected for molecular characterization and expression analysis. This was carried out using in four rice lines comprising the parents and two BC₂F₇ lines from a local rice breeding program, with Malaysian rice variety, Oryza sativa ssp. indica cv. MR219 as recurrent parent, and Oryza rufipogon (IRGC105491) as donor parent. Approximately 2.8 kb and 2.6 kb putative RPK1 cDNA nucleotide sequences were obtained from Oryza rufipogon and Oryza sativa ssp. indica cv. MR219 respectively. However, only 402 …

Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari

Student Works (2010-2019)

The selectable marker system based on the Escherichia coli phosphomannose isomerase (pmi) gene was adapted for genetic transformation of oil palm. This system makes use of the pmi gene that encodes phosphomannose isomerase, which converts mannose-6-phosphate to fructose-6-phosphate and uses mannose as the selection agent. This is to anticipate the future requirement of using non-antibiotic resistance genes for the commercialization of transgenic oil palm. The use of antibiotic or herbicide-based selection systems has caused much public concern due to inadequate knowledge of the agents’ impact on the environment and on human health. In this study, four transformation vectors, namely pMI3, …

Computational Identification Of Micro Rna In Musa Sp Est Sequences., Tan Yew Seong

Student Works (2010-2019)

MicroRNAs (miRNAs) are small single-stranded non-protein-coding RNAs of about 22 nucleotides that play an important role in post-transcriptional gene regulation in animals and plants by targeting mRNAs for direct cleavage of mRNAs or repression of mRNA translation. In this study, a homology search based approach has been used for identifying the novel miRNA genes of Musa sp. using the BLAST program with a total of 42,978 Musa sp. expressed sequence tags (EST) were compared to a total of 959 previously known plant miRNA sequences. Forty-seven candidate miRNA with homology to previously known miRNAs were identified. By using the mFold3.1 program, …

Spring Seed Guide 2010: Nebraska Variety And Hybrid Tests -- Soybeans, Teshome Regassa, Robert N. Klein, Bruce Anderson, Charles A. Shapiro, Jim Krall

Department of Agronomy and Horticulture: Faculty Publications

NEBRASKA SOYBEAN VARIETY TESTS 2009

Crop Production Summary: The November 2009 estimated soybean yield for Nebraska was 52 bushels per acre from 4.800 million harvested acres. The total production of soybeans for the state was forecasted at 247 million bushels. These estimates are from the November National Agricultural Statistics Service.

Studies On Ecophysiological Responses Of Durum Wheat Genotypes Under Water Deficit Conditions., Ali Akbar Moayedi

Student Works (2010-2019)

Water is the main component in the make-up of plants and serves as the solvent, which transports minerals and dissolved carbohydrates throughout the plant. However, the ecological and physiological responses of plants to water limitation vary at different developmental stages and limits crop production and yield in crops. Therefore, investigation of the plant response to drought at its different developmental stages can be useful to overcome the negative effects of water stress in different plant growth stages. In addition, although, drought stress has been well documented as an effective parameter in decreasing crop production in arid and semi arid regions, …