Plant Breeding and Genetics Commons^™

Identifying Physiological, Morphological, And Genetic Drivers Of Key Intermediary Phenotypic Traits Associated With Stalk Lodging Resistance Within Genetically Diverse Maize Germplasm And Sorghum, Norbert Bokros

Theses and Dissertations--Plant and Soil Sciences

Maize stalk lodging reduces grain yields due to the permanent breakage or deformation of stalk tissue before grain harvest. To reveal new insights into the complex problem of maize stalk lodging, this dissertation will examine three primary objectives. First, the direct, diurnal influence of turgor pressure will be shown to have only marginal effects on stalk bending strength, a key trait associated with stalk lodging resistance. Up to a dozen individual stalks, grown to physiological maturity within the same field, were tested using a DARLING testing device. In parallel, stalk turgor status as measured by leaf water potential and turgor …

Evaluation Of Five Roma Tomato Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker

Midwest Vegetable Trial Reports

A Roma tomato cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). HM Claus (HM), PanAmerican (PA), and Sakata (SK) seed companies donated five Roma tomato cultivars for plastic-mulch bedded and trellised hand harvest. Overall trial quality was excellent, despite a dry start that brought in insects, and hard rains that flared bacterial diseases.

Evaluation Of 13 Round Tomato Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker

Midwest Vegetable Trial Reports

A round tomato cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). Bejo (BJ), Enza Zaden (EZ), Seminis (SM), Sakata (SK), and Seedway (SW) seed companies donated round tomato cultivars for plastic-mulch bedded and trellised hand harvest. Overall trial quality was excellent, despite a dry start that brought in insects, and hard rains that flared bacterial diseases.

Evaluation Of Five Cauliflower Cultivars For Summer Harvest In Southwest Michigan In 2023, Ben Phillips, Leah Freeman

Midwest Vegetable Trial Reports

A cauliflower cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). Bejo (BJ), Clifton (CL), Sakata (SK), and Tozer (TZ) seed companies donated five cauliflower cultivars for plastic-mulch bedded hand harvest. The trial quality was good for collecting data on stressed plants. A dry start and cabbage maggot attack reduced stand with replacement plants lagging behind, and heat around head formation created a high proportion of non-marketable heads.

Evaluation Of 11 Broccoli Cultivars For Summer Harvest In Southwest Michigan In 2023, Ben Phillips, Leah Freeman

Midwest Vegetable Trial Reports

A broccoli cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). Bejo (BJ), Clifton (CL), Sakata (SK), and Tozer (TZ) seed companies donated 11 broccoli cultivars for plastic-mulch bedded hand harvest. The trial quality was good for collecting data on stressed plants. A dry start and cabbage maggot attack reduced stand with replacement plants lagging behind, and heat around head formation created a high proportion of non-marketable heads.

Evaluation Of Six Slicing Cucumber Cultivars In Southwest Michigan In 2023, Ben Phillips, Jenny Schoonmaker

Midwest Vegetable Trial Reports

A slicing cucumber cultivar trial was planted at the Southwest Michigan Research and Extension Center (42.081985, -86.354087, Benton Harbor, Michigan). Clifton (CL), Seedway (SW), Seminis (SM), and Syngenta (SY) seed companies donated five slicing cucumber cultivars for plastic-mulch bedded hand harvest. The trial quality was OK, with a dry start that created an uneven stand, and some deer browse.

Soybean Genetics, Genomics, And Breeding For Improving Nutritional Value And Reducing Antinutritional Traits In Food And Feed, William M. Singer, Yi-Chen Lee, Zachary Shea, Caio Canella Vieira, Dongho Lee, Xiaoying Li, Mia Cunicelli, Shaila S. Kadam, Mohammad Amir Waseem Khan, Grover Shannon, M. A. Rouf Mian, Henry T. Nguyen, Bo Zhang

Agriculture Faculty Publications

Soybean [Glycine max(L.) Merr.] is a globally important crop due to its valuable seed composition, versatile feed, food, and industrial end-uses, and consistent genetic gain. Successful genetic gain in soybean has led to widespread adaptation and increased value for producers, processors, and consumers. Specific focus on the nutritional quality of soybean seed composition for food and feed has further elucidated genetic knowledge and bolstered breeding progress. Seed components are historical and current targets for soybean breeders seeking to improve nutritional quality of soybean. This article reviews genetic and genomic foundations for improvement of nutritionally important traits, such as protein and …

Origin, Rise And Development Of American Upland Cotton And Their Status At Present. Second Edition, Ed. By F.M. Bourland, J. O. Ware

AAES Research Reports and Research Bulletins

American Upland cotton (Gossypium hirsutum) is a Dixie product. Although the stocks of the species were brought from elsewhere, new types (through series of adaptational changes) formed this distinctive group—the final characteristics of which are a product of the Cotton Belt of the United States. These biological processes were considerably aided by man and the steps of development possibly were about as follows: (a) natural selection took place in the earlier introductions, (b) seed were saved from the more choice stocks, (c) series of subsequent introductions were obtained from the better sources, (d) these were acclimatized and the superior ones …

Arkansas Soybean Research Studies 2022, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading soybean-producing state in the mid-southern United States. Arkansas ranked 11th in soybean production in 2022 when compared to the other soybean-producing states in the U.S. The state represented 3.04% of the total U.S. soybean production and 3.64% of the total acres planted in soybean in 2022. The 2022 state soybean average yield was 52.0 bushels per acre, tying the previous state yield record of 52 bushels per acre set in 2021. The top five soybean-producing counties in 2022 were Mississippi, Crittenden, Phillips, Poinsett, and Arkansas (Table 1). These five counties accounted for over 35.7% of the …

Advances And Opportunities In Unraveling Cold-Tolerance Mechanisms In The World's Primary Staple Food Crops, Sofora Jan, Sachin Rustgi, Rutwik Barmukh, Asif B. Shikari, Brenton Leske, Amanuel Bekuma, Darshan L. Sharma, Wujun Ma, Upendra Kumar, Uttam Kumar, Abhishek Bohra, Rajeev K. Varshney, Reyazul Rouf Mir

Grain and Other Field Crops Research Articles

Temperatures below or above optimal growth conditions are among the major stressors affecting productivity, end-use quality, and distribution of key staple crops including rice (Oryza sativa), wheat (Triticum aestivum), and maize (Zea mays L.). Among temperature stresses, cold stress induces cellular changes that cause oxidative stress and slowdown metabolism, limit growth, and ultimately reduce crop productivity. Perception of cold stress by plant cells leads to the activation of cold-responsive transcription factors and downstream genes, which ultimately impart cold tolerance. The response triggered in crops to cold stress includes gene expression/suppression, the accumulation of sugars upon chilling, and signaling molecules, among …

Arkansas Wheat Performance Tests 2022-2023, J. F. Carlin, R. B. Mulloy, R. D. Bond

Arkansas Agricultural Experiment Station Research Series

Wheat variety performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture’s Arkansas Crop Variety Improvement Program.

The tests provide information to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for small-grain producers. The tests were conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Pine Tree Research Station near Colt, and the Rohwer Research Station near Rohwer. Specific location and cultural practice information accompany each …

Summaries Of Arkansas Cotton Research 2022, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

The current economic environment continues to drive the need to produce record or near-record yields to be profitable. Price volatility in 2022 added another level of difficulty in the quest for being profitable. The cotton market saw significant movement after reaching a $1.5802 per pound high in May 2022, cotton prices corrected and more than halved in value at the October 2022 low, and then consolidated around the 80 cents per pound level in December 2022 (Cotton is Consolidating). Great uncertainties still exist for the upcoming season, most of which are outside of our control. These include, but are not …

Exome-Based New Allele-Specific Pcr Markers And Transferability For Sodicity Tolerance In Bread Wheat (Triticum Aestivum L.), Roopali Bhoite, Rosemary Smith, Urmil Bansal, Mirza Dowla, Harbans Bariana, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Targeted exome-based genotype by sequencing (t-GBS), a sequencing technology that tags SNPs and haplotypes in gene-rich regions was used in previous genome-wide association studies (GWAS) for sodicity tolerance in bread wheat. Thirty-nine novel SNPs including 18 haplotypes for yield and yield-components were identified. The present study aimed at developing SNP-derived markers by precisely locating new SNPs on ~180 bp allelic sequence of t-GBS, marker validation, and SNP functional characterization based on its exonic location. We identified unknown locations of significant SNPs/haplotypes by aligning allelic sequences on to IWGSC RefSeqv1.0 on respective chromosomes. Eighteen out of the target 39 SNP locations …

Genetic Resources And Precise Gene Editing For Targeted Improvement Of Barley Abiotic Stress Tolerance, Sakura Karunarathne, Esther Walker, Darshan Sharma, Chengdao Li, Yong Han

Grain and Other Field Crops Research Articles

Abiotic stresses, predominately drought, heat, salinity, cold, and waterlogging, adversely affect cereal crops. They limit barley production worldwide and cause huge economic losses. In barley, functional genes under various stresses have been identified over the years and genetic improvement to stress tolerance has taken a new turn with the introduction of modern gene-editing platforms. In particular, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is a robust and versatile tool for precise mutation creation and trait improvement. In this review, we highlight the stress-affected regions and the corresponding economic losses among the main barley producers. We collate about …

Genomic Approaches To Enhance Adaptive Plasticity To Cope With Soil Constraints Amidst Climate Change In Wheat, Roopali Bhoite, Yong Han, Alamuru Krishna Chaitanya, Rajeev K. Varshney, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Climate change is varying the availability of resources, soil physicochemical properties, and rainfall events, which collectively determines soil physical and chemical properties. Soil constraints—acidity (pH < 6), salinity (pH ≤ 8.5), sodicity, and dispersion (pH > 8.5)—are major causes of wheat yield loss in arid and semiarid cropping systems. To cope with changing environments, plants employ adaptive strategies such as phenotypic plasticity, a key multifaceted trait, to promote shifts in phenotypes. Adaptive strategies for constrained soils are complex, determined by key functional traits and genotype × environment × management interactions. The understanding of the molecular basis of stress tolerance is particularly challenging for plasticity traits. Advances in sequencing and high-throughput genomics …

Genome-Wide Association Study For Agronomic Traits In Bermudagrass (Cynodon Spp.), Lovepreet Singh

Theses and Dissertations

Bermudagrass (Cynodon spp.) breeding and cultivar development is hampered by limited information regarding its genetic and phenotypic diversity. A germplasm collection of 206 bermudagrass accessions from 29 countries was genotyped with high-throughput genotyping-by-sequencing technique. Genomic diversity in this diverse germplasm panel was assessed with multifaceted approaches including population structure, phylogenetic analysis, principal component analysis, and genetic diversity parameters. This study revealed substantial genetic variation in the Cynodon accessions, demonstrating the potential of this germplasm panel for further genetic studies and cultivar development in breeding programs. Another critical issue in turfgrass breeding is the lack of information regarding the genetic …

Investigating Effect Of Seed Source And Developing Germination Protocols To Improve Success In Restoration Of Arkansas Tallgrass Prairies, Rhiannon Spencerosa

Graduate Theses and Dissertations

American tallgrass prairie has faced losses estimated at 87-98% of original land area since European settlement. Native seeds are often used to supplement struggling or extinct plant populations in tallgrass prairie restoration and establishment sites. Two main considerations in restoration and establishment are from where to source seed and how to obtain high germination rates. In order to determine the effect of seed source, a common garden experiment was performed in Fayetteville, Arkansas in 2022 with five prairie species: Andropogon gerardii (big bluestem), Bouteloua curtipendula (sideoats grama), Panicum virgatum (switchgrass), Schizachyrium scoparium (little bluestem), and Sorghastrum nutans (Indiangrass). Seed was …

Arkansas Cotton Variety Test 2022, F. Bourland, A. Beach, B. Milano, B. Guest, C. Kennedy, L. Martin, B. Robertson

Arkansas Agricultural Experiment Station Research Series

The primary goal of the Arkansas Cotton Variety Test is to provide unbiased data regarding the agronomic performance of cotton varieties and advanced breeding lines in the major cotton-growing areas of Arkansas. This information helps seed companies establish marketing strategies and assists producers in choosing varieties to plant. These annual evaluations will then facilitate the inclusion of new, improved genetic material in Arkansas cotton production. Adaptation of varieties is determined by evaluating the lines at five University of Arkansas System Division of Agriculture research sites (Manila, Keiser, Judd Hill, Marianna, and Rohwer). The 2022 tests at Rohwer were adversely affected …

An International Wheat Diversity Panel Reveals Novel Sources Of Genetic Resistance To Tan Spot In Australia, Julian Taylor, Dorthe Jorgensen, Caroline S. Moffat, Ken J. Chalmers, Rebecca Fox, Grant J. Hollaway, Melissa J. Cook, Stephen M. Neate, Pao Theen See, Manisha Shankar

Biosecurity Research Articles

Tan spot is a foliar disease in wheat caused by the fungal pathogen Pyrenophora tritici-repentis (Ptr) and has been reported to generate up to 50% yield losses under favourable disease conditions. Although farming management practices are available to reduce disease, the most economically sustainable approach is establishing genetic resistance through plant breeding. To further understand the genetic basis for disease resistance, we conducted a phenotypic and genetic analysis study using an international diversity panel of 192 wheat lines from the Maize and Wheat Improvement Centre (CIMMYT), the International Centre for Agriculture in the Dry Areas (ICARDA) and Australian (AUS) wheat …

Analysis And Installation Of A Demonstration Agroforestry Orchard For Californian Mediterranean Plant Communities, Brandon Hurd

Master of Science in Environmental Sciences and Management Projects

Climate-appropriate agroforestry can provide low-input food security and ecosystem services for local Californian Mediterranean climates, while conserving natural resources (e.g., water, nitrogen, etc.). This project showcases a variety of agroforestry methods for five common plant communities of California and other analogous Mediterranean climates at the CAFES Experimental Farm on the campus of Cal Poly San Luis Obispo. Plant community species and their ethnobotanical uses were analyzed to mimic and incorporate aspects of native flora. Agricultural plants were also characterized to represent each of the five selected plant communities. GIS was used to assess the project site for soil, slope, and …

Validation Of Newly Designed Ssr Markers For Eight Rice (Oryza Sativa L.) Genotypes With Variable Heat Tolerance Responses Based On Agromorphic Data And Pollen Fertility Analysis [Research Note], Monaliza B. Magat, Norvie L. Manigbas, Jessica D. Rey

The Philippine Agricultural Scientist

Rice is one of the most valuable staple food crops in the world. However, several challenges greatly affect production, one of which is the threat imposed by heat stress. To address this, researchers are developing varieties that are heat stress tolerant with genetic markers aid. In this study, eight rice genotypes, namely Dular, Nagina 22, NSIC Rc 222, Milyang 23, EL15, EL92, EL85, and IR52 were observed for agromorphic data, which included plant height, panicle length, filled and unfilled grains, and grain yield. Flower samples were collected to determine the effect of heat stress on pollen fertility. Molecular markers were …

M. ‘Teresita Lantin-Rosario’ And M. ‘Dolores A. Ramirez’ – New Varieties Of Mussaenda [Research Note], Gerlie P. Antesco, Jennelyn C. Bengoa, Maria Luisa D. Guevarra

The Philippine Agricultural Scientist

An outstanding selections of Mussaenda developed in the Institute of Plant Breeding (IPB), College of Agriculture and Food Science (CAFS), University of the Philippines Los Baños (UPLB) were registered to the National Seed Industry Council as new varieties. These are Mussaenda ‘Teresita Lantin-Rosario’ and Mussaenda ‘Dolores A. Ramirez’, named after an ornamental plant breeder and a National Scientist, respectively, in recognition to their significant contributions in the fields of genetics and plant breeding. Mussaenda ‘Teresita Lantin-Rosario’ is a hybrid and a progeny from a cross between M. ‘Maria Makiling’ and M. ‘Diwata’. The calyx lobes are all petaloid, numerous, and …

Morphological And Genetic Analysis Of The Root System In Two American Grapevines (Vitis Species), Parinaz Mohtasebi

Graduate Theses/Dissertations

The North American grapes species Vitis rupestris Scheele and Vitis riparia Michx have been the pillars of rootstock breeding for many decades. Though a large body of viticultural knowledge has been accumulated on their impact on grafted scions, the genetic basis of their root system architecture (RSA) has received limited scientific attention. In this study, I generated and analyzed adventitious root systems from dormant cuttings of 22 V. riparia and 19 V. rupestris accessions, as well as 162 interspecific F1 hybrid progeny from a cross between V. rupestris (♀) and V. riparia (♂). I photographed the roots and then extracted …

Genome-Wide Association Studies And Advanced Genomic Selection Strategies: Towards The Optimization Of Oat (Avena Sativa L) Breeding, Girma Ayana

Electronic Theses and Dissertations

Oats are important cereal crops worldwide, primarily valued for their nutritional and health benefits. The primary goal of oat breeding programs is to accelerate genetic gain to develop oat varieties with the desired product profiles, with the aim of meeting the needs and expectations of growers, processors, and consumers. Achieving this goal involves conducting extensive and resource-intensive field trials, particularly for traits such as crop yield and grain quality, which are strongly influenced by environmental factors. The availability of cost-effective molecular markers and tools like genome-wide association studies (GWAS) and genomic selection have the potential to accelerate the oat breeding …

Improving Baking Quality Of Soft Red Winter Wheat In Kentucky Through Breeding And Sulfur-Nitrogen Fertility Management, Maria Paula Castellari

Theses and Dissertations--Plant and Soil Sciences

Soft Red Winter (SRW) wheat is an important crop grown on about 400,000 acres in Kentucky as a pivotal cash crop in the 3-crops-in-2-years rotation of corn, wheat, and soybean. The humid environment of Kentucky generates a high yielding wheat crop, with low protein concentration and weak gluten strength. This wheat is mainly sold to millers and used by the industry for cakes, pastries, cookies, and crackers. Conversely, there is a rapidly growing interest in identifying other value-added markets for this wheat different to commodity commercialization, such as artisan-baked goods and craft-distilled beverages. Evaluating the quality characteristics of wheat grown …

New Semi-Dwarfing Alleles With Increased Coleoptile Length By Gene Editing Of Gibberellin 3-Oxidase 1 Using Crispr-Cas9 In Barley (Hordeum Vulgare L.), Jingye Cheng, Camilla Hill, Yong Han, Tianhua He, Xingguo Ye, Sergey Shabala, Ganggang Guo, Meixue Zhou, Ke Wang, Chengdao Li

Grain and Other Field Crops Research Articles

The green revolution was based on genetic modification of the gibberellin (GA) hormone system with “dwarfing” gene mutations that reduces GA signals, conferring shorter stature, thus enabling plant adaptation to modern farming conditions. Strong GA-related mutants with shorter stature often have reduced coleoptile length, discounting yield gain due to their unsatisfactory seedling emergence under drought conditions. Here we present gibberellin (GA) 3-oxidase1 (GA3ox1) as an alternative semi-dwarfing gene in barley that combines an optimal reduction in plant height without restricting coleoptile and seedling growth. Using large-scale field trials with an extensive collection of barley accessions, we showed that a natural …

Successful Creation Of Seedless (Sterile) Leucaena Germplasm Developed From Interspecific Hybridisation For Use As Forage, Daniel Real, Clinton K. Revell Dr, Yong Han, Chengdao Li, Marieclaire Castello, C. D. Bailey

Grain and Other Field Crops Research Articles

Context: The legume shrub/tree leucaena (Leucaena leucocephala (Lam.) de Wit.) is highly regarded as a cattle fodder, although it is considered an environmental weed in many parts of Australia.

Aims: We investigated the feasibility of developing a forage variety of triploid leucaena through interspecific hybridisation that is sterile (without seeds) as a strategy to mitigate its weed risk.

Methods: A conventional breeding strategy was used to create triploid hybrids from hand-crosses between tetraploid and diploid species of leucaena. Molecular markers were developed to confirm successful crosses and flow cytometry was used to confirm the ploidy level. The plants are being …

Arkansas Soybean Research Studies 2021, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading soybean-producing state in the mid-southern United States. Arkansas ranked 11th in soybean production in 2021 compared to the other soybean-producing states in the U.S. The state represented 3.49% of the total U.S. soybean production and 3.49% of the total acres planted in soybean in 2021. The 2021 state soybean average yield was 52.0 bushels per acre, setting a new state record and surpassing the previous yield record of 51.5 bushels per acre set in 2020. The top five soybean-producing counties in 2021 were Mississippi, Phillips, Crittenden, Poinsett, and Arkansas (Table 1). These five counties accounted for …

Arkansas Wheat Performance Tests 2021-2022, J. F. Carlin, R. B. Morgan, R. D. Bond, D. E. Moon

Arkansas Agricultural Experiment Station Research Series

Wheat variety performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station, Department of Crop, Soil, and Environmental Sciences. The tests provide information to companies developing varieties and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for small-grain producers. The tests are conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Pine Tree Research Station near Colt, and the Rohwer Research Station near Rohwer. Specific location …

Summaries Of Arkansas Cotton Research 2021, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

While the basic growth and development of the cotton plant have not changed significantly in recent history, the business of cotton production is ever-changing. The last two years have seen us plant a crop just about as late as we thought possible, yet extended favorable conditions at season's end have been our salvation, helping to lead us to record yields. The economic environment over the last few years has been such that farmers need to produce record or near-record yields to advance. Unfortunately, production levels at the state yield average barely cover out-of-pocket expenses.

Great uncertainties exist for the upcoming …