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Full-Text Articles in Other Plant Sciences

Effects Of Heavy Metal Uptake On Growth, Chlorophyll Content, And Calcium Oxalate Crystals In Lemna Minor (Duckweed), Olivia F. Miller Apr 2023

Effects Of Heavy Metal Uptake On Growth, Chlorophyll Content, And Calcium Oxalate Crystals In Lemna Minor (Duckweed), Olivia F. Miller

Undergraduate Honors Thesis Projects

Lemna minor L., duckweed, is a common aquatic plant used for the phytoremediation of wastewater. Heavy metal contamination poses a significant issue, and numerous studies have been conducted on the efficiency of L. minor’s hyperaccumulation ability of these metals. Calcium oxalate crystals are an important aspect of the growth and development of L. minor, but how they are influenced by the uptake of heavy metal has not been extensively studied. This study aims to determine the effects that lead, cadmium, and copper have on the formation of calcium oxalate crystals, vegetative growth, and chlorophyll a and b content on L. …


Quantifying The Carbon Stored And Sequestered By The Trees On Pomona College’S Campus, Paola A. Giron-Carson Jan 2023

Quantifying The Carbon Stored And Sequestered By The Trees On Pomona College’S Campus, Paola A. Giron-Carson

Scripps Senior Theses

We are experiencing a climate crisis that must be confronted with strategic mitigation. Pomona College contributes to the climate crisis through its emissions for which there is a baseline record. However there is no baseline record of the climate mitigation currently performed by the trees on Pomona’s campus through carbon storage. This study seeks to determine a current baseline quantity of carbon stored and sequestrated by Pomona’s trees as well as possible courses of climate mitigation for Pomona College to take. Initial information gathering was conducted through interviews with several stakeholders. This study was conducted using data collected prior to …


Flora Of Doe Mountain Recreation Area, Johnson County, Tennessee, Benjamin Mccullough Aug 2022

Flora Of Doe Mountain Recreation Area, Johnson County, Tennessee, Benjamin Mccullough

Electronic Theses and Dissertations

A botanical inventory of Doe Mountain Recreation Area (DMRA) in northeastern Tennessee was conducted to help guide conservation-based management. A total of 484 species were found in DMRA, comprising 94 families, and 285 genera, 10 species listed in the state rare plant list, and 76 exotic species. Two species, Liatris virgata and Lycopodiella inundata, were new state records. Water in the Lycopodiella seep was an order of magnitude more acid than at other sites. An analysis of the wildland-urban interface showed that only 13% of the area was classified as uninhabited. The inventory-invasion index, introduced to quantify the relative …


The Influence Of Water Stress And Rootstocks On Nutrient Uptake And Gas Exchange Of Young Peach Trees, Naif Alshammari Aug 2022

The Influence Of Water Stress And Rootstocks On Nutrient Uptake And Gas Exchange Of Young Peach Trees, Naif Alshammari

All Theses

Young peach trees are often rainfed in the southeastern U.S. and periods of dry weather can cause tree water deficit that can be detrimental to orchard productivity. There is a lack of understanding of the influence of water regimes and rootstocks on the nutritional status and gas exchange of the peach tree. Most peach growers in Southeastern United States do not irrigate their young peach trees because they do not produce fruit until the third leaf. In this region, peach growers use Guardian as a rootstock, although MP-29 is also interesting for growers with orchards that have Armillaria root rot …


Reassessment Of Species Boundaries And Phylogenetic Relationships In The Desmodium Ciliare Complex (Fabaceae) Using Morphological And Dna Data, Joshua Wilkinson May 2022

Reassessment Of Species Boundaries And Phylogenetic Relationships In The Desmodium Ciliare Complex (Fabaceae) Using Morphological And Dna Data, Joshua Wilkinson

Master's Theses

Desmodium (Beggar's ticks) is a generally weedy genus of approximately 280 species in the angiosperm family Fabaceae (Subfamily Papilionoideae, Tribe Desmodieae) characterized by indehiscent loments constricted into segments. Within the Southeastern U.S., the Desmodium ciliare group is one of two Desmodium species complexes that have been historically difficult, with much argument about the limits of species and the role of hybridization. There are three commonly recognized species within the D. ciliare group, D. ciliare (Muhl. ex Willd.) DC., Desmodium marilandicum (L.) DC., and D. obtusum (Muhl. ex Willd.) DC., with two supposed hybrids in addition to the …


Getting To The Root Cause: The Genetic Underpinnings Of Root System Architecture And Rhizodeposition In Sorghum, Farren Smith Jan 2022

Getting To The Root Cause: The Genetic Underpinnings Of Root System Architecture And Rhizodeposition In Sorghum, Farren Smith

Graduate Theses, Dissertations, and Problem Reports

Plants are some of the most diverse organisms on earth, consisting of more than 350,000 different species. To understand the underlying processes that contributed to plant diversification, it is fundamental to identify the genetic and genomic components that facilitated various adaptations over evolutionary history. Most studies to date have focused on the underlying controls of above-ground traits such as grain and vegetation; however, little is known about the “hidden half” of plants. Root systems comprise half of the total plant structure and provide vital functions such as anchorage, resource acquisition, and storage of energy reserves. The execution of these key …


The Impacts Of Maple Syrup Production On The Herbaceous Layer In Appalachian Hardwood Forests, Logan Scott Ferguson Jan 2022

The Impacts Of Maple Syrup Production On The Herbaceous Layer In Appalachian Hardwood Forests, Logan Scott Ferguson

Graduate Theses, Dissertations, and Problem Reports

Vegetation data were collected from eight maple syrup farms (sugarbushes) and eight undeveloped maple-dominated sites to examine potential differences in understory plant communities due to disturbance effects. Understory plants were identified to the species level and percent cover of aboveground leaf-area for each species was estimated within 600 total quadrats. Overstory data and environmental data were collected to help determine if they had any effect on herbaceous-layer plant communities. Species richness, Shannon diversity, and Pielou’s evenness were calculated, with the analysis showing no significant differences between the site types. The absolute cover of plant functional groups was also compared between …


Three Lc-Ms Plant Metabolomics Studies Of Hop (Humulus) Species: Wild H. Neomexicanus, Drought Stress, And Agricultural Terroir, Taylan Morcol Sep 2021

Three Lc-Ms Plant Metabolomics Studies Of Hop (Humulus) Species: Wild H. Neomexicanus, Drought Stress, And Agricultural Terroir, Taylan Morcol

Dissertations, Theses, and Capstone Projects

The hop plant (Humulus L., Cannabaceae) is a dioecious, perennial, twining vine with a long history of human use. Nowadays, hop plants are generally grown for their inflorescences (“cones”), which are used in brewing for their phytochemical metabolites. Many of these metabolites are involved in plant stress response and communication. Genetics and environment are two major factors that affect plant metabolism. In three separate metabolomics studies, this project examined the effects of both genetic and environmental factors on hop phytochemistry.

In the first study, 23 hop genotypes were grown in two different locations in the Pacific Northwest region of …


Responses Of The Carnivorous Pink Spoonleaf Sundew (Drosera Capillaris) To Nitrogen Addition, Phosphorus Addition, And Simulated Fire, Karina Rodriguez Castillo May 2021

Responses Of The Carnivorous Pink Spoonleaf Sundew (Drosera Capillaris) To Nitrogen Addition, Phosphorus Addition, And Simulated Fire, Karina Rodriguez Castillo

Honors Theses

This study investigated how the carnivorous plant, Drosera capillaris(pink sundew), responded to changes in the resources of its habitat, the fire-prone, nutrient-poor wet savannas. It is of interest to determine what soil nutrient(s), nitrogen or phosphorus, limits the survival and growth of pink sundew, and to what extent, if any, does fire affect the survival and growth of this carnivorous plant. My field experiments had twenty plots with two replicate groups consisting of four pink sundews in each plot. From the fall of 2017 to the fall of 2019, only half of the plots were clipped annually, and there …


Three Centuries Of Vegetation Change In The William & Mary College Woods Reconstructed Using Phytoliths, Timothy Terlizzi May 2021

Three Centuries Of Vegetation Change In The William & Mary College Woods Reconstructed Using Phytoliths, Timothy Terlizzi

Undergraduate Honors Theses

The College Woods, west of William & Mary’s campus, consists of ~900 acres of protected southern mixed hardwood forest. The woods surround Lake Matoaka, a former millpond established in ~1700. Despite the rich history of the area, little is known about how the dominant vegetative landcover has shifted over the last 300 years. This study set out to quantify the modern vegetation within the College Woods via the phytolith assemblages within the soil and identify shifts in the assemblages since the creation of Lake Matoaka and whether these changes are distinct from the vegetation that existed in the area before …


A Floristic Survey Of Andalusia: A Historic Preservation Site In Baldwin County, Georgia, William Pharr May 2020

A Floristic Survey Of Andalusia: A Historic Preservation Site In Baldwin County, Georgia, William Pharr

Biology Theses

A survey of the vascular plants of Andalusia, a historic preservation site located along Highway 441 north of Milledgeville in Baldwin County, Georgia, was conducted between August 2018 and February 2020. The site was the late home of esteemed author, Flannery O’Connor, and resides along the Georgia fall line, making it a historically significant area that could yield a unique plant community convergence, as well as rare endemic species. This floristic inventory provides baseline data on the species and plant communities of Andalusia for use in future studies regarding the flora of this area. A total of 191 vascular species …


Vines In The Neotropics: Phylogenomics, Biogeography And Systematics In Passion Flowers (Passiflora Subgenus Decaloba Section Decaloba), Serena Acha Nov 2019

Vines In The Neotropics: Phylogenomics, Biogeography And Systematics In Passion Flowers (Passiflora Subgenus Decaloba Section Decaloba), Serena Acha

Dissertations

With 600 species, Passiflora is a large, morphologically complex and broadly distributed genus in Passifloraceae that represents a major challenge to scientists interested in understanding the evolutionary history of tropical vines. Passiflora has been divided into subgenera, super sections and sections. One of the most enigmatic and species-rich (~120 spp.) groups in Passiflora is section Decaloba, which occurs in the Neotropics and is particularly diverse in Andean montane forests. In this study, we used phylogenomic and population genomic approaches to investigate the evolutionary history, biologeography, species boundaries, and taxonomy of Passiflora section Decaloba. We sampled herbarium specimens, extracted …


The Effects Of Clipping On The Biomass Production Of Native Warm Season Grasses On Reclaimed Abandoned Coal Mine Soils, James A K Daniel Jul 2019

The Effects Of Clipping On The Biomass Production Of Native Warm Season Grasses On Reclaimed Abandoned Coal Mine Soils, James A K Daniel

Electronic Theses & Dissertations

THE EFFECTS OF CLIPPING ON THE BIOMASS PRODUCTION OF NATIVE

WARM SEASON GRASSES ON RECLAIMED

ABANDONED COAL MINE SOILS

James A K Daniel

Strip mining leaves behind highly disrupted plant and soil communities. Mined land reclamation returns the land back to a natural or economically usable state, however, reclamation cannot completely restore the soils to their original state. Subsequently, normal frequencies of management practices may not be as effective as in undisturbed soils. Understanding how the severity of soil disturbance affects plant production is important for habitat rehabilitation and determining effective management techniques to be implemented following reclamation. This project …


Warm Stratification Increases Germination Of Dendromecon Rigida And Ehrendorferia Chrysantha, Cesar Garcia Jun 2019

Warm Stratification Increases Germination Of Dendromecon Rigida And Ehrendorferia Chrysantha, Cesar Garcia

Electronic Theses, Projects, and Dissertations

We explored the seasonal factors that might play a role in triggering germination of Dendromecon rigidaand Ehrendorferia chrysantha.D. rigida and E. chrysantha have been found difficult to germinate using common greenhouse techniques, Keeley and Fotheringham successfully germinated both species after storing their seeds in the field over a year and treating them with smoke. Identifying the specific seasonally changing factors that stimulated germination could have implications for understanding germination requirements for these and other hard-to-germinate chaparral species.

Dendromecon rigidaand Ehrendorferia chrysanthaare part of the Papaveraceae family and both are found in the chaparral environment in …


Fire And Road Disturbance Impacts On Forest Plant Species And Seed Rain In Table Mountain Fire Arai, Kittitas County, Washington, Jonathan A. Betz Jan 2019

Fire And Road Disturbance Impacts On Forest Plant Species And Seed Rain In Table Mountain Fire Arai, Kittitas County, Washington, Jonathan A. Betz

All Master's Theses

Forest communities are in a constant state of change. Disturbance events can alter the physical landscape and create conditions favorable to some species while negatively impacting others. Fire has been a natural, reoccurring source of disturbance in Pacific Northwest forests. Over the past centuries the fire paradigm has changed in favor of fire suppression. Forest roads permit access provide greater access but further fracture forest community’s continuity. The 2012 Table Mountain Fire and road influence have altered the plant community’s seed rain and vegetation. This study measured dispersed seeds and understory vegetation cover as functions of roads and fire intensity. …


The Fate Of Xylem-Transported Co2 In Plants, Samantha S. Stutz, David T. Hanson Jul 2018

The Fate Of Xylem-Transported Co2 In Plants, Samantha S. Stutz, David T. Hanson

Biology ETDs

The concentration of carbon dioxide in tree stems can be ~30-750 times higher than current atmospheric [CO2]. Dissolved inorganic carbon enters the xylem from root and stem respiration and travels with water through the plant. However, the fate of much of this xylem-transported CO2 is unknown. In these studies I examined the fate of xylem-transported CO2 traveling through the petiole and leaf. This was accomplished by placing cut leaves from a woody and herbaceous C3 species, and a Kranz-type C4 species, in a solution of dissolved NaH13CO3 at concentrations similar to …


A Contribution Toward A Global Monograph Of Gyroporus: Taxonomy, Phylogeny, Biogeography, Naveed Davoodian May 2018

A Contribution Toward A Global Monograph Of Gyroporus: Taxonomy, Phylogeny, Biogeography, Naveed Davoodian

Dissertations, Theses, and Capstone Projects

Gyroporus (Sclerodermatineae, Boletales, Agaricomycetes, Basidiomycota, Fungi) is a genus of ectomycorrhizal mushroom-forming fungi distributed throughout the world in suitable habitats. Previous attempts to untangle the diversity of this genus proved difficult due to the presence of semi-cryptic species and equivocal results from phylogenetic analysis of ribosomal RNA markers. To overcome these obstacles, a combined taxonomic and phylogenetic (emphasizing protein-coding genes) approach is used here to delimit species and elucidate geographic and evolutionary patterns of Gyroporus. Careful study of relevant literature and herbarium specimens was augmented by field work in North America, Australia, and East Asia for observation and collection …


A New Anatomically-Preserved Plant From The Lower Devonian Of Quebec (Canada): Implications For Euphyllophyte Phylogeny And Early Evolution Of Structural Complexity, Selin Toledo Jan 2018

A New Anatomically-Preserved Plant From The Lower Devonian Of Quebec (Canada): Implications For Euphyllophyte Phylogeny And Early Evolution Of Structural Complexity, Selin Toledo

Cal Poly Humboldt theses and projects

An abrupt transition in the fossil record between Early Devonian plants with simple structure and structurally-complex later Devonian plants, has frustrated efforts to understand patterns of phylogeny across the Early/Middle Devonian boundary and the evolution of complex forms. Both these aspects have important implications for lignophyte and seed plant evolution. In the first chapter, I evaluate phylogenetic relationships between the earliest seed plants, Aneurophytales, and Stenokoleales, using comprehensive taxon sampling (28 species, including all relevant permineralized species) and a set of 40 discrete and nine continuous morpho-anatomical characters. Analysis of this dataset supports the three traditional taxonomic groups (seed plants, …


Phenotypic Variation And Genetic Purity Of The Original 'Prime-Jim®' X 'Arapaho' Population, Loren M. Luther May 2017

Phenotypic Variation And Genetic Purity Of The Original 'Prime-Jim®' X 'Arapaho' Population, Loren M. Luther

Horticulture Undergraduate Honors Theses

In 2013, Castro et al., produced the first linkage map for primocane-fruiting blackberries using the cross ‘Prime-Jim®’ x ‘Arapaho.’ This mapping population has been maintained since the original experiment with the hopes of conducting future studies. Further research was determined to be needed on the population to better characterize additional phenotypic traits, and since nine years had passed since the seedlings were established, there was a need to assess the continued genetic purity of the population using molecular methods. Phenotypic data was collected to analyze the variation of the population including soluble solids content, titratable acidity, average weight, shape, and …


Salvinia Molesta: An Assessment Of The Effects And Methods Of Eradication, Arti Lal Dec 2016

Salvinia Molesta: An Assessment Of The Effects And Methods Of Eradication, Arti Lal

Master's Projects and Capstones

Salvinia molesta is an invasive aquatic fern. It is now the second worse aquatic invader in the world. Since the 1930s, it has invaded most tropical and some temperate countries. S. molesta plants grow vegetatively and can increase in size rapidly. S. molesta can form thick mats of up to 1-meter-thick. There are a number of ways these thick mats negatively affect the environment: 1) reduce light to benthic organisms, 2) reduce oxygen in the water column for other organisms, 3) accumulate as organic matter at the bottom of the water column, 4) decrease nutrients for other organisms, and 5) …


Demography And Disease Of The Rare Shrub Buckleya Distichophylla (Santalaceae) In Northeastern Tennessee, William Seth Ratliff Dec 2015

Demography And Disease Of The Rare Shrub Buckleya Distichophylla (Santalaceae) In Northeastern Tennessee, William Seth Ratliff

Undergraduate Honors Theses

Piratebush (Buckleya distichophylla (Nutt.) Torr.) is a rare, hemiparasitic shrub with the only extant populations in western North Carolina, northeastern Tennessee, and southwestern Virginia. The preferred natural hosts of piratebush, Carolina and eastern hemlocks, have seen sharp declines over the last decade due to the invasive hemlock woolly adelgid. Virginia pine, another important host of piratebush, is also susceptible to disease, specifically Cronartium appalachianum, a rust fungus for which piratebush is the secondary host. This study described and analyzed current demographic parameters of three Tennessee piratebush populations. Additionally, spatial patterns of disease and demographic characters were analyzed. These …


A Microhabitat Assessment Of Five Species Of Invasive Plants In The Ozarks And Appalachians, Eric Hearth May 2015

A Microhabitat Assessment Of Five Species Of Invasive Plants In The Ozarks And Appalachians, Eric Hearth

Graduate Theses and Dissertations

Invasive species present a threat to native communities and their introduction and expansion can alter community structure and dynamics. Multiple approaches can be employed for invasive species management including prevention and detection. In this study, microhabitat assessments were conducted on colonies of five species of invasive plants, Alliaria petiolata (M. Bieb.) Cavara & Grande, Lespedeza cuneata (Dum. Cours.) G. Don, Lonicera japonica Thunb., Microstegium vimineum (Trin.) A. Camus, and Rosa multiflora Thunb. in the Ozark Plateau and Appalachians. Elevation, soil moisture, soil pH, light ratio, slope, aspect, distance to disturbance, as well as soil nutrient levels were recorded for each …


Dispersal: A Multidisciplinary Investigation Of Plant Life, Alexandra E. Arzt Jan 2015

Dispersal: A Multidisciplinary Investigation Of Plant Life, Alexandra E. Arzt

Theses and Dissertations

Using plants as a basis for exploring the interstices between the human and nonhuman, this thesis investigates ideas of awareness, intelligence, deep time, animism, and the fluctuating human perception of the agency of Nature. It outlines environmental art practices since the 1950s involving vegetal life. In addition, the paper provides a critical analysis of plant perception of Jakob von Uexküll’s work and theories of vital materialism and “critical plant studies” while noting recent studies in plant neurobiology. In my work, plants become active participants via their movement, seeding, and smell. This study takes the form of imitation, purposeful symbiosis, anthropomorphism, …


Evaluating Pollination Ecology Of The Endangered Pityopsis Ruthii (Small) Small (Asteraceae), Philip Anthony Moore May 2014

Evaluating Pollination Ecology Of The Endangered Pityopsis Ruthii (Small) Small (Asteraceae), Philip Anthony Moore

Masters Theses

Pityopsis ruthii (Small) Small, also known as Ruth’s golden aster, is a federally endangered herbaceous perennial, endemic to two river systems, the Hiwassee and the Ocoee, within the Cherokee National Forest, Polk County, Tennessee. There are approximately 13,000 individuals that may be at high risk of short-term extirpation (Thompson and Schwartz, 2006). Little is known of the basic reproduction and life history of P. ruthii. Clebesh and Sloan (1993), Cruzan (2001), Park (1998), and Wadl et al. (2014) found evidence that seed production and seed viability are highly variable. Clebesh and Sloan (1993) indicated that pollinator visitation was highly temporal …


Factors Influencing The Establishment And Survival Of Native Hardwood Tree Seedlings Of The Kentucky Inner Bluegrass Blue Ash-Oak Savanna-Woodland, James D. Shaffer Jan 2013

Factors Influencing The Establishment And Survival Of Native Hardwood Tree Seedlings Of The Kentucky Inner Bluegrass Blue Ash-Oak Savanna-Woodland, James D. Shaffer

Theses and Dissertations--Biology

Historically, the Kentucky Inner Bluegrass blue ash-oak savanna-woodland was the primary ecosystem of the Inner Bluegrass Region (IBR) of Kentucky. After European settlement, the majority (>99%) of Bluegrass savanna was converted to agricultural and urban land uses. Currently remnant savanna tree species are failing to recruit. Therefore, a long-term restoration ecology project researching competition and disturbance on seedling establishment, survival, and growth has been established at Griffith Woods (the largest remaining savanna in Kentucky) in Harrison Co., KY. Fourteen native hardwood tree species (a total of 6,168 seedlings) have been experimentally planted. Light, soil, surrounding vegetation, and herbivory, factors …


Regalia-Induced Resistance In Powdery Mildew-Infected Grapevine Plants Correlates With The Induction Of Chalcone Synthase, Thomas Crottogini Dec 2010

Regalia-Induced Resistance In Powdery Mildew-Infected Grapevine Plants Correlates With The Induction Of Chalcone Synthase, Thomas Crottogini

Horticulture and Crop Science

No abstract provided.


The Vascular Flora Of Greater San Quintín, Baja California, Mexico, Sula E. Vanderplank Jan 2010

The Vascular Flora Of Greater San Quintín, Baja California, Mexico, Sula E. Vanderplank

CGU Theses & Dissertations

The plants of San Quintín (Baja California, Mexico) were documented through intensive fieldwork and the collection of herbarium specimens to create a checklist of species. This region is home to a diverse flora with high levels of local endemism and many rare plants. The flora documented in this study was compared to historical records from the region and shows the impact of agriculture and urbanization on the plants, including several extirpated species. A study of the perennial vegetation using a 1 km grid provides species distribution data for 140 native species, which were assessed to highlight areas of significant species …


The Development Of A Gc/Ms Protocol For The Analysis Of Polysaccharides In Echinacea Purpurea, Michaela Knapp Apr 2007

The Development Of A Gc/Ms Protocol For The Analysis Of Polysaccharides In Echinacea Purpurea, Michaela Knapp

Undergraduate Theses and Capstone Projects

Echinacea purpurea is an herbal supplement used to reduce symptoms of common colds and flu-like illnesses. As of right now, it is not well understood what chemical components in the plant are immunologically active. The four main potentially immunologically active chemical components in E. purpurea are caffeic acid derivatives, alkamides, polyacetylenes, and polysaccharides. In this investigation, a protocol was developed in an attempt to identify polysaccharides present in Puritan’s Pride E. purpurea (aerial) “non-irradiated” herbal supplement First, an estimate of the crude total polysaccharide content in Puritan’s Pride and in a ChromaDex certified botanical standard (E. purpurea -powdered root) was …


A Medicinal Plant Survey In Marketplaces Of Ecuador, Pamela Bailey Jan 1999

A Medicinal Plant Survey In Marketplaces Of Ecuador, Pamela Bailey

Theses, Dissertations and Capstones

A medicinal herb survey was conducted in ten major marketplaces in Andean and coastal communities of Ecuador. Collections were made during the summer of 1997. Four plant collections from each market were pressed, dried, identified, and the medicinal information for each plant was recorded. Photographs of fresh plants, market vendors and the marketplaces were taken.

Fifty three families were represented in the 112 species collected. Forty-two species (37%) were introduced to Ecuador while seventy species (63%) were native to Ecuador. Plant taxa were grouped into 22 categories of medicinal usage. The most common 3 uses were: 1. General tonic and …


Biological Species Concepts In Eastern North American Populations Of Lentinellus Ursinus, Andrew N. Miller Jan 1997

Biological Species Concepts In Eastern North American Populations Of Lentinellus Ursinus, Andrew N. Miller

Masters Theses

As part of a biosystematic study of the Lentinellus ursinus species complex, field and herbarium specimens from North America, Central America, and Europe were examined. Single basidiospore isolates from basidiomata collected in Illinois, Indiana, Iowa, Michigan, Minnesota, Missouri, Tennessee, North Carolina, Wisconsin, Costa Rica, and Austria were crossed in all possible combinations to determine the mating system operating in each population. All populations exhibited bifactorial or tetrapolar mating systems. Tester strains were then crossed to identify intercompatible populations and to determine if morphological species in the L. ursinus species complex are congruent with biological species. Three biological species were recognized …