Horticulture Commons^™

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such …

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, …

Potential For Crop Production Increase In Argentina Through Closure Of Existing Yield Gaps, Fernando Aramburu Merlos, Juan Pablo Monzon, Jorge L. Mercau, Miguel Taboada, Fernando H. Andrade, Antonio J. Hall, Esteban Jobbagy, Kenneth Cassman, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Favorable climate and soils for rainfed crop production, together with a relatively low population density,results in 70–90% of Argentina grain production being exported. No assessment to date has tried to estimate the potential for extra grain production for soybean, wheat and maize, which account for 78%of total harvested area, by yield gap closure on existing cropland area and its impact at a global scale.The objectives of this paper are (i) to estimate how much additional grain could be produced without expanding crop area by closing yield gaps in Argentina, (ii) to investigate how this production and yield gaps varies across …

How Do Various Maize Crop Models Vary In Their Responses To Climate Change Factors?, Simon Bassu, Nadine Brisson, Jean-Louis Durand, Kenneth J. Boote, Jon Lizaso, James Jones, Cynthia Rosenzweig, Alex Ruane, Myriam Adam, Christian Baron, Bruno Basso, Christian Biernath, Hendrick Boogaard, Sjaak Conijn, Marc Corbeels, Delphine Deryng, Giacomo De Sanctis, Sebastian Gayler, Patricio Grassini, Jerry Hatfield, Steven Hoek, Cesar Izaurralde, Raymond Jongschaap, Armen Kemanian, Christian Kersebaum, Soo-Hyung Kim, Naresh Kumar, David Makowski, Christoph Muller, Claas Nendel, Eckart Priesack, Maria Virinia Pravia, Federico Sau, Iurii Shcherbak, Fulu Tao, Edmar Teixeira, Dennis Timlin, Katharina Waha

Department of Agronomy and Horticulture: Faculty Publications

Potential consequences of climate change on crop production can be studied using mechanistic crop simulation models. While a broad variety of maize simulation models exist, it is not known whether different models diverge on grain yield responses to changes in climatic factors, or whether they agree in their general trends related to phenology, growth, and yield. With the goal of analyzing the sensitivity of simulated yields to changes in temperature and atmospheric carbon dioxide concentrations [CO2], we present the largest maize crop model intercomparison to date, including 23 different models. These models were evaluated for four locations representing a wide …

Automated Conserved Non-Coding Sequence (Cns) Discovery Reveals Differences In Gene Content And Promoter Evolution Among Grasses, Gina Marie Turco, James C. Schnable, Brent S. Pedersen, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Conserved non-coding sequences (CNS) are islands of non-coding sequence that, like protein coding exons, show less divergence in sequence between related species than functionless DNA. Several CNSs have been demonstrated experimentally to function as cis-regulatory regions. However, the specific functions of most CNSs remain unknown. Previous searches for CNS in plants have either anchored on exons and only identified nearby sequences or required years of painstaking manual annotation. Here we present an open source tool that can accurately identify CNSs between any two related species with sequenced genomes, including both those immediately adjacent to exons and distal sequences separated by …

Arbuscular Mycorrhizal Fungi Differ In Their Ability To Regulate The Expression Of Phosphate Transporters In Maize (Zea Mays L.), Hui Tian, Rhae A. Drijber, Xiaolin Li, Daniel N. Miller, Brian J. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Previous studies have found that some phosphate (Pi) starvation inducible transporter genes are downregulated and arbuscular mycorrhizal (AM) inducible Pi transporter genes are upregulated in maize roots associated with the fungus Glomus intraradices. However, little is known about the functional diversity of different AM fungal species in influencing the expression of Pi transporters in maize roots. Here, we studied the expression of two Pi transporter genes ZEAma:Pht1;3 (Pi starvation inducible) and ZEAma:Pht1;6 (AM inducible) in maize root colonized by different AM fungal inoculants. Non-mycorrhizal maize, maize colonized by Glomus deserticola (CA113), Glomus intraradices (IA506), Glomus mosseae (CA201), Gigaspora gigantea …

Optimizing Greenhouse Corn Production: How Can Two-Spotted Spider Mites Be Controlled?, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This publication describes our experiences controlling mites while conducting experiments to optimize greenhouse corn production. Controlled studies were not conducted, but we recommend cultural practices of water spray-offs of leaf undersides and the stripping lower leaves as the crop ages. Also recommended are applications of beneficial insect Phytoseiulus persimilis at very high rates. The rates are an update of our previous publication. Photos of the pest and cultural practices are provided.

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site …

Optimizing Greenhouse Corn Production: What Is The Best Pot Size?, Derek Gambrel, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This publication describes studies conducted to determine best pot size for optimizing greenhouse corn production. We suggest a 2.2-gallon container without large drainage holes extending up the side of the pot. This represents an update of our previous recommendation of a smaller pot, after observing instability in row planting configurations. Photos are included of corn in various size pots.

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled …

Optimizing Greenhouse Corn Production: Summary, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This publication provides a summary of the goals and scope of our studies to optimize greenhouse corn production, as well as a single-page chart of all the recommendations. The chart includes recommendations for substrate, container, irrigation, fertilization, spacing and lighting. In this version, we updated the pot size recommendation.

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and …

Optimizing Greenhouse Corn Production: References, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: Will Using Calcined Clay Reduce Fungus Gnat Infestation?, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: Can More Than One Plant Be Grown Per Pot?, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: What Is The Best Open Pollination Method?, Adam Leonberger, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: What Prevents Calcium Deficiency?, Jeannie Ross, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: Materials And Methods, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Purdue Methods: How Do I Prevent Calcined Clay From Leaking Out Of Container?, Justin Kottkamp, Tyler Mason, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: What Is The Best Lighting And Plant Density?, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: What If Roots Need To Be Clean For Analysis?, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: What Other Ideas For Improving Seed Yield?, Adam Leonberger, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: What Is The Best Fertilizer Formulation And Strength?, Justin Kottkamp, Arek Varjabedian, Jeannie Ross, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: What Is The Best Irrigation Strategy?, Westin Rink, Derek Gambrel, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: Can Growth Regulators Be Used To Keep Plants Short?, Craig Schluttenhofer, Jeannie Ross, Tyler Mason, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: Can Seed Yield Be Improved Using Cold-Stored Tassels?, Tyler Mason, Jeannie Ross, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Optimizing Greenhouse Corn Production: What Is The Best Root Medium?, Derek Gambrel, Westin Rink, Tyler Mason, Justin Kottkamp, Arek Varjabedian, Jeannie Ross, Robert Eddy, Daniel T. Hahn

Purdue Methods for Corn Growth

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.

Inoculation With Arbuscular Mycorrhizal Fungi Or Crop Rotation With Mycorrhizal Plants Improves The Growth Of Maize In Limed Acid Sulfate Soil, Hasao Higo, Katsunori Isobe, Dong-Jin Kang, Kazuhiro Ujie, Rhae A. Drijber, Ryuichi Ishii

Department of Agronomy and Horticulture: Faculty Publications

Arbuscular mycorrhizal fungi (AMF) improve the uptake of immobile mineral nutrients such as phosphate, thereby improving plant growth. In acid sulfate soil (ASS), AMF spore density is generally low which impacts root colonization and phosphate uptake. Thus, inoculation may help increase AMF colonization of crops grown in ASS. AMF spore density decreases after cultivation of a non-host crop or bare fallow. In addition, preceding crops affect the growth and yield of subsequent crops. The production of AMF inocula requires AMF-compatible plants. The objective of the present study is to elucidate the effect of preceding crops on the persistence of inoculated …