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2022

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Articles 1 - 30 of 243

Full-Text Articles in Horticulture

Transformation And Gene Editing In The Bioenergy Grass Miscanthus, Anthony Trieu, Mohammad B. Belaff, Pradeepa Hirannaiah, Shilpa Manjunatha, Rebekah Wood, Yokshitha Bathula, Rebecca L. Billingsley, Anjali Arpan, Erik J. Sacks, Thomas E. Clemente, Stephen P. Moose, Nancy A. Reichert, Kankshita Swaminathan Dec 2022

Transformation And Gene Editing In The Bioenergy Grass Miscanthus, Anthony Trieu, Mohammad B. Belaff, Pradeepa Hirannaiah, Shilpa Manjunatha, Rebekah Wood, Yokshitha Bathula, Rebecca L. Billingsley, Anjali Arpan, Erik J. Sacks, Thomas E. Clemente, Stephen P. Moose, Nancy A. Reichert, Kankshita Swaminathan

Department of Agronomy and Horticulture: Faculty Publications

Background: Miscanthus, a C4 member of Poaceae, is a promising perennial crop for bioenergy, renewable bioproducts, and carbon sequestration. Species of interest include nothospecies M. x giganteus and its parental species M. sacchariforus and M. sinensis. Use of biotechnology-based procedures to genetically improve Miscanthus, to date, have only included plant transformation procedures for introduction of exogenous genes into the host genome at random, non-targeted sites.

Results: We developed gene editing procedures for Miscanthus using CRISPR/Cas9 that enabled the mutation of a specific (targeted) endogenous gene to knock out its function. Classified as paleo-allopolyploids (duplicated ancient …


Yield Prediction Through Integration Of Genetic, Environment, And Management Data Through Deep Learning, Daniel R. Kick, Jason G. Wallace, James C. Schnable, Judith M. Kolkman, Barış Alaca, Timothy M. Beissinger, Jode Edwards, David Ertl, Sherry Flint-Garcia, Joseph L. Gage, Candice N. Hirsch, Joseph E. Knoll, Natalia De Leon, Dayane C. Lima, Danilo E. Moreta, Maninder P. Singh, Addie Thompson, Teclemariam Weldekidan, Jacob D. Washburn Dec 2022

Yield Prediction Through Integration Of Genetic, Environment, And Management Data Through Deep Learning, Daniel R. Kick, Jason G. Wallace, James C. Schnable, Judith M. Kolkman, Barış Alaca, Timothy M. Beissinger, Jode Edwards, David Ertl, Sherry Flint-Garcia, Joseph L. Gage, Candice N. Hirsch, Joseph E. Knoll, Natalia De Leon, Dayane C. Lima, Danilo E. Moreta, Maninder P. Singh, Addie Thompson, Teclemariam Weldekidan, Jacob D. Washburn

Department of Agronomy and Horticulture: Faculty Publications

Accurate prediction of the phenotypic outcomes produced by different combinations of genotypes, environments, and management interventions remains a key goal in biology with direct applications to agriculture, research, and conservation. The past decades have seen an expansion of new methods applied toward this goal. Here we predict maize yield using deep neural networks, compare the efficacy of 2 model development methods, and contextualize model performance using conventional linear and machine learning models. We examine the usefulness of incorporating interactions between disparate data types. We find deep learning and best linear unbiased predictor (BLUP) models with interactions had the best overall …


Establishment Of The Invasive Cactus Moth, Cactoblastis Cactorum (Berg) (Lepidoptera: Pyralidae) In Pakistan: A Potential Threat To Cultivated, Ornamental And Wild Opuntia Spp. (Cactaceae), Muhammad Ather Rafi, Harry Pavulaan, Muhammad Islam, Muhammad Ashfaq, Haseeb Kamran, Walija Fayaz, Gul Naz Parveen, Riffat Sultana, Ahmad Zia, Waqar Ahmed, Qudrat Ullah, Muhammad Qasim, Falak Naz, Nazeer Ahmed, Muhammad Tariq Khan, Muhammad Saeed, Jalal Hayat Khan Dec 2022

Establishment Of The Invasive Cactus Moth, Cactoblastis Cactorum (Berg) (Lepidoptera: Pyralidae) In Pakistan: A Potential Threat To Cultivated, Ornamental And Wild Opuntia Spp. (Cactaceae), Muhammad Ather Rafi, Harry Pavulaan, Muhammad Islam, Muhammad Ashfaq, Haseeb Kamran, Walija Fayaz, Gul Naz Parveen, Riffat Sultana, Ahmad Zia, Waqar Ahmed, Qudrat Ullah, Muhammad Qasim, Falak Naz, Nazeer Ahmed, Muhammad Tariq Khan, Muhammad Saeed, Jalal Hayat Khan

The Taxonomic Report of the International Lepidoptera Survey

Subsequent to the significant accomplishment of biological control of Opuntia weeds in Australia, the larvae of the cactus moth, Cactoblastis cactorum (native to parts of South America), were released in many countries for the biological control of native Opuntia species (Simmonds and Bennett, 1966). Inauspiciously, larvae were also released in the Caribbean, where the moth spread naturally and by the human support all over the region (García-Turudi et al., 1971). Its enhanced dissemination rate and the biological potential for invasiveness, suggests that the cactus moth is likely to become an invasive pest of Opuntia in the Southeast United States, Mexico, …


Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail Dec 2022

Genetic Variation In Common Bunt Resistance In Synthetic Hexaploid Wheat, Amira M. I. Mourad, Alexey Morgounov, P. Stephen Baenziger, Samar M. Esmail

Department of Agronomy and Horticulture: Faculty Publications

Common bunt (caused by Tilletia caries and T. Foetida) is a major wheat disease. It occurs frequently in the USA and Turkey and damages grain yield and quality. Seed treatment with fungicides is an effective method to control this disease. However, using fungicides in organic and low-income fields is forbidden, and planting resistant cultivars are preferred. Due to the highly effective use of fungicides, little effort has been put into breeding resistant genotypes. In addition, the genetic diversity for this trait is low in modern wheat germplasm. Synthetic wheat genotypes were reported as an effective source to increase the …


Colored Sweet Bell And Tapered Pepper Cultivar Evaluation For High Tunnel Production In West-Central Indiana, 2022, Petrus Langenhoven, Lian Mahorith Duron Alvarado, Eduardo Jose Miranda Oviedo Dec 2022

Colored Sweet Bell And Tapered Pepper Cultivar Evaluation For High Tunnel Production In West-Central Indiana, 2022, Petrus Langenhoven, Lian Mahorith Duron Alvarado, Eduardo Jose Miranda Oviedo

Midwest Vegetable Trial Reports

Colored sweet bell-shaped and tapered peppers are a staple at the farmers' market. Many small and medium-sized farming operations in Indiana grow this summer crop. High tunnels allow growers to protect their crops and extend their growing season. Sweet peppers, in particular, benefit from the unique growing environment created by the high tunnel. Planting of peppers can start at least 2-4 weeks earlier in the spring, and production can continue into the fall until the first hard freeze. Pepper variety performance data for Indiana is not readily available. We are working hard to change that. Currently, we have evaluated twenty-five …


Inheritance Of 2,4‑Dichlorophenoxyacetic Acid (2,4‑D) Resistance In Amaranthus Palmeri, Chandrima Shyam, Dallas E. Peterson, Amit J. Jhala, Mithila Jugulam Dec 2022

Inheritance Of 2,4‑Dichlorophenoxyacetic Acid (2,4‑D) Resistance In Amaranthus Palmeri, Chandrima Shyam, Dallas E. Peterson, Amit J. Jhala, Mithila Jugulam

Department of Agronomy and Horticulture: Faculty Publications

In this study, the inheritance of 2,4-D resistance in a multiple herbicide-resistant Palmer amaranth (KCTR) was investigated. Direct and reciprocal crosses were performed using 2,4-D-resistant KCTR and susceptible KSS plants to generate F1 progenies. 2,4-D dose–response assays were conducted to evaluate the response of progenies from each F1 family along with KCTR and KSS plants in controlled environmental growth chambers. Additionally, 2,4-D-resistant male and female plants from each of the F1 families were used in pairwise crosses to generate pseudo-F2 families. Segregation (resistance or susceptibility) of progenies from the F2 families in response to a …


A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace Dec 2022

A Stealth Health Approach To Dietary Fibre, P. Stephen Baenziger, Katherine Anna Frels, Steve Greenspan, Julie Jones, Alison Lovegrove, Devin J. Rose, Peter Shewry, Rod Wallace

Department of Agronomy and Horticulture: Faculty Publications

Average dietary fibre intakes have increased little in the past twenty years in many countries, including the USA1 . Multi-million-dollar campaigns promoting fruits, vegetables, whole grains and other foods high in fibre have delivered only small changes in diets2 , and consumers have not changed from traditional staples to whole-grain options3 . UK millers report that consumption of whole-wheat bread has actually declined over the past decade (P. Shewry, personal communication). In the US, white flour, which is lower in fibre than whole-wheat flour, accounts for nearly 40% of the fibre intake4 . We believe that …


Respon Pertumbuhan Tanaman Krisan Pot Terhadap Hormon Giberelin Dan Waktu Pemangkasan Yang Berbeda, Galuh Wulan Andini, Refa Firgiyanto Dec 2022

Respon Pertumbuhan Tanaman Krisan Pot Terhadap Hormon Giberelin Dan Waktu Pemangkasan Yang Berbeda, Galuh Wulan Andini, Refa Firgiyanto

Jurnal Kultivasi

Salah satu teknologi yang dapat digunakan untuk meningkatkan kualitas tanaman krisan (Dendrathema grandifloria) pot yaitu dengan pemberian giberelin dan pengaturan waktu pemangkasan. Tujuan pemberian giberelin dan pengaturan waktu pemangkasan adalah untuk memacu pertumbuhan dengan merangsang perpanjangan batang serta memunculkan tunas lateral sehingga memperbanyak percabangan yang akhirnya dapat menghasilkan banyak kuntum bunga. Penelitian ini bertujuan untuk mendapatkan konsentrasi giberelin dan waktu pemangkasan yang terbaik. Penelitian dilaksanakan pada 3 Juli sampai 4 September 2020 di Greenhouse Rembangan, Desa Kemuning Lor, Kecamatan Arjasa, Jember. Rancangan percobaan yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) Faktorial dengan 2 faktor yang diulang 3 …


Aplikasi Kalium Klorida Pada Dosis Yang Berbeda Secara Fertigasi Tetes Untuk Meningkatkan Pertumbuhan Dan Hasil Bawang Merah, Alfu Laila, Nur Iman Muztahidin, Dwiki Radinal, Andi Apriany Fatmawaty, Nuniek Hermita Dec 2022

Aplikasi Kalium Klorida Pada Dosis Yang Berbeda Secara Fertigasi Tetes Untuk Meningkatkan Pertumbuhan Dan Hasil Bawang Merah, Alfu Laila, Nur Iman Muztahidin, Dwiki Radinal, Andi Apriany Fatmawaty, Nuniek Hermita

Jurnal Kultivasi

Budidaya bawang merah (Allium cepa L.) di Provinsi Banten terhambat oleh masalah kekurangan air karena diusahakan di lahan kering. Oleh karena hal tersebut, kami mengevaluasi pengaruh pemberian kalium klorida (KCl) melalui fertigasi tetes untuk efisiensi penggunaan air dan nutrisi pada budidaya bawang merah di lahan kering Banten. Penelitian polybag dilaksanakan dengan rancangan acak kelompok lengkap (RAKL) dengan 4 ulangan sebagai blok. Perlakuan terdiri dari dosis KCl dengan system fertigasi tetes yakni 50, 75, 100, 125 dan 150% dari dosis rekomendasi KCl untuk Provinsi Banten dan aplikasi KCl dengan sistem irigasi konvensional sesuai dosis rekomendasi sebagai kontrol. Hasil penelitian menunjukkan bahwa …


Genetic Variations Of Narcissus Tazetta And Selected Narcissus Cultivars Based On The Sequence Analysis Of Nrits And TrnL‑Is‑TrnF Regions, Seo Young Park, Mi Jin Jeon, Young Hee Joung, Keenan L. Amundsen, Gianluca Burchi, Angelo Porcelli, Mark S. Roh Dec 2022

Genetic Variations Of Narcissus Tazetta And Selected Narcissus Cultivars Based On The Sequence Analysis Of Nrits And TrnL‑Is‑TrnF Regions, Seo Young Park, Mi Jin Jeon, Young Hee Joung, Keenan L. Amundsen, Gianluca Burchi, Angelo Porcelli, Mark S. Roh

Department of Agronomy and Horticulture: Faculty Publications

Narcissus tazetta var. chinensis (N. taz. var. chi.) accessions collected from the coastal areas of China, Korea, and Japan were compared with accessions of lower ranks of Narcissus tazetta (N. taz.) from Italy to study the genetic variations and with several morphologically similar cultivars available in the trade. Sequences of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrITS) dendrogram and the chloroplast trnL-IS-trnF intergeneric spacer-gene (cpIS) were analyzed by maximum likelihood (ML) and posterior probability using STRUCTURE program. All single fower form of N. taz. var. chi. collected from China, Korea, and …


Distribution And Soil Characteristics Associated With The Neches River Rose-Mallow (Hibiscus Dasycalyx), Morgan E. Langlinais Dec 2022

Distribution And Soil Characteristics Associated With The Neches River Rose-Mallow (Hibiscus Dasycalyx), Morgan E. Langlinais

Electronic Theses and Dissertations

The Neches River rose-mallow, Hibiscus dasycalyx, is a rare herbaceous perennial endemic to East Texas. The threatened, obligate wetland species is currently at risk of being lost in the wild due to habitat loss, encroachment of nonnative and native plant species, and hybridization with two other Hibiscus congeners, Hibiscus laevis and Hibiscus moscheutos. Known locations of each of the three Hibiscus species were used to measure select soil characteristics and habitat variables associated with each of the rose-mallows. H. dasycalyx was concluded to be associated with more fertile soils and slightly more canopy coverage than H. laevis and …


Teknik Fermentasi Campuran Bahan Organik Sebagai Sumber Nutrisi Organik Pada Sayuran Sawi Yang Ditanam Dengan Hidroponik, Darwin H. Pangaribuan, Yohannes Cahya Ginting, Rugayah, Purba Sanjaya, Agus Karyanto, Kurnia Cahyani Dewi, Intan Puspita Sari Dec 2022

Teknik Fermentasi Campuran Bahan Organik Sebagai Sumber Nutrisi Organik Pada Sayuran Sawi Yang Ditanam Dengan Hidroponik, Darwin H. Pangaribuan, Yohannes Cahya Ginting, Rugayah, Purba Sanjaya, Agus Karyanto, Kurnia Cahyani Dewi, Intan Puspita Sari

Jurnal Kultivasi

Nutrisi organik untuk budidaya sistem sumbu hidroponik merupakan salah satu alternatif untuk mengatasi kendala mahalnya harga pupuk anorganik. Penelitian ini bertujuan untuk mengetahui metode ekstraksi terbaik antara metode air hangat, air dingin, dan fermentasi kemudian menentukan waktu fermentasi terbaik dari unsur hara organik campuran rumput laut, sabut kelapa dan daun kelor yang diharapkan memiliki kandungan nutrisi organik yang mendekati dengan formula nutrisi anorganik. Penelitian ini terdiri atas 2 percobaan: (i) percobaan pertama meneliti jenis metode ekstraksi, dan (ii) percobaan kedua meneliti waktu fermentasi terbaik. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan faktor tunggal dan diulang sebanyak 6 …


Non-Traditional Adjuvants And Methods For Applying Root-Promoting Compounds In Commercial Cutting Propagation, Anthony Turner Bowden Dec 2022

Non-Traditional Adjuvants And Methods For Applying Root-Promoting Compounds In Commercial Cutting Propagation, Anthony Turner Bowden

Theses and Dissertations

The nursery/greenhouse industry is innovative. There have been several reports in the popular literature of non-traditional adjuvants used in plant propagation; however, these claims have never been evaluated in a scientific setting. Five studies, conducted at the South Mississippi Branch Experiment Station in Poplarville in 2019 and 2021 determined the impact that adding honey to water-soluble auxin solutions and surfactants to foliar applied auxin solutions on physiological responses associated with adventitious root formation. Treatments in studies presented in chapters two and three included three honey sources (multiflora, local, and Manuka). Honey-infused water-soluble auxin solutions affected plant species but not in …


Evaluation Of Warm Season Vegetables Using Sustainable Production Practices, Jacob Arthur Dec 2022

Evaluation Of Warm Season Vegetables Using Sustainable Production Practices, Jacob Arthur

Theses and Dissertations

Sustainable practices were evaluated for the production of two warm season vegetable crops, tomato and chile peppers. The first study investigated the plant vegetative growth, fruit production timing, yield components and fruit quality of three hybrid and three heirloom tomato cultivars grown in a high tunnel using grafted and non-grafted plants. Grafting the selected tomato cultivars with the two rootstock types did not alter total marketable yield of any cultivar, but affected overall stem diameter, fruit color, and β-carotene concentrations of tested tomato cultivars. The second study evaluated the plant growth, yield, and fruit quality of nine heirloom chile pepper …


Application Of Organic Fertilizer And Phosphate Solubilizing Bacteria To Increase The Growth And Yield Of Potatoes In Andisol, Agustina Erlinda Marpaung, Bina Karo, Susilawati Barus, Rina Christina Hutabarat, Rasiska Tarigan Dec 2022

Application Of Organic Fertilizer And Phosphate Solubilizing Bacteria To Increase The Growth And Yield Of Potatoes In Andisol, Agustina Erlinda Marpaung, Bina Karo, Susilawati Barus, Rina Christina Hutabarat, Rasiska Tarigan

Jurnal Kultivasi

Andisol has low phosphate (P) nutrient content due to high P fixation by allophane minerals, which affects crop production. The application of organic fertilizers and phosphate solubilizing bacteria can increase the availability of P in the soil in order to increase the production of potato plants. This study aimed to evaluate the effect of different types of organic fertilizer and phosphate solubilizing bacteria (PSB) on potato production. The research was conducted from August to November 2020 in the installation of research and assessment of agricultural technology Berastagi, Karo Regency, with an altitude of ± 1,340 m above sea level and …


Poisson Hurdle Model-Based Method For Clustering Microbiome Features, Zhili Qiao, Elle Barnes, Susannah Tringe, Daniel P. Schachtman, Peng Liu Dec 2022

Poisson Hurdle Model-Based Method For Clustering Microbiome Features, Zhili Qiao, Elle Barnes, Susannah Tringe, Daniel P. Schachtman, Peng Liu

Department of Agronomy and Horticulture: Faculty Publications

Motivation: High-throughput sequencing technologies have greatly facilitated microbiome research and have generated a large volume of microbiome data with the potential to answer key questions regarding microbiome assembly, structure and function. Cluster analysis aims to group features that behave similarly across treatments, and such grouping helps to highlight the functional relationships among features and may provide biological insights into microbiome networks. However, clustering microbiome data are challenging due to the sparsity and high dimensionality.

Results: We propose a model-based clustering method based on Poisson hurdle models for sparse microbiome count data. We describe an expectation–maximization algorithm and a …


In Vitro Rooting Techniques In Prunus Spp. For Propagation And Disease Screening For Armillaria Root Rot (Arr) Resistance, John Lawson Dec 2022

In Vitro Rooting Techniques In Prunus Spp. For Propagation And Disease Screening For Armillaria Root Rot (Arr) Resistance, John Lawson

All Theses

Prunus is a genus widely cultivated to produce edible fruit including almond (P. amygdalus), peach (P. persica (L.) Batsch), cherries (P. avium and P. cerasus), among others. The cultivation of Prunus is economically important for several regions of the United States and relies on appropriate cultivars and rootstocks that are adapted to a growing region. Encroaching plant pathogens are forcing breeders to use sexually compatible germplasm from related species for introgression of novel alleles that confer tolerance or resistance. This is especially true in the rootstock breeding where interspecific hybridization is often used …


A Glass Bead Semi-Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez, Karin V. Van Dijk Dec 2022

A Glass Bead Semi-Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez, Karin V. Van Dijk

Department of Agronomy and Horticulture: Faculty Publications

Background: Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudate collection. Here, we present a newly designed semi-hydroponic system that uses glass beads as solid support to simulate soil impedance, which combined with drip irrigation, facilitates growth of healthy maize plants, collection and analysis of root exudates, and phenotyping of the roots with minimal growth disturbance or root damage. Results: This system was used to collect root exudates from seven maize genotypes using water or 1 mM CaCl2, …


Nitrogen Management In Rice Under Suboptimal Soil Conditions, Payton Suzanne Rhea Dec 2022

Nitrogen Management In Rice Under Suboptimal Soil Conditions, Payton Suzanne Rhea

Graduate Theses and Dissertations

In Mid-South rice (Oryza sativa, L.) production, nitrogen (N) fertilizer management for pureline varieties is most often recommended as a single preflood (SPF) or two-way split (2WS) application in a direct-seeded, delayed-flood system. Most of the N fertilizer is typically applied at the four- to six-leaf stage onto dry soil, and the second application, if necessary, into the floodwater during early reproductive growth stages (referred to as midseason). Environmental factors frequently prohibit growers from applying early N fertilizer under optimal dry soil conditions. A study was conducted to determine the best N fertilization management practices to utilize in rice when …


Unveiling The Potential Of Calcium And Natamycin For Botrytis Blight Management On Cut Roses, Melissa Munoz Dec 2022

Unveiling The Potential Of Calcium And Natamycin For Botrytis Blight Management On Cut Roses, Melissa Munoz

All Dissertations

Botrytis blight caused by the fungus Botritys cinerea is the most devastating disease of cut roses. The extensive use of fungicides used for Botrytis blight management during cut rose production and postharvest represents a severe threat in terms of fungicide resistance development as has been previously reported. Additionally, health concerns for growers and the environment are growing in recent years making consumers more aware of the fungicides in the products that they consume including ornamentals. This scenario highlights the importance of searching for alternative products to synthetic fungicides. During this research, the use of calcium as an alternative management strategy …


Addressing Challenges Of Dryland Production Of Sunflowers And Corn In The Semi-Arid High Plains Of Nebraska, Zhan Orazov Dec 2022

Addressing Challenges Of Dryland Production Of Sunflowers And Corn In The Semi-Arid High Plains Of Nebraska, Zhan Orazov

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Corn and sunflower are value crops for America. Cultivation of corn and sunflower often vary depending on growing environment. Selecting appropriate planting dates, hybrids and plant density frequently concerns farmers. This concern is understandable because the decision made directly impacts final income. The objectives were to evaluate interaction of corn and sunflower planting dates and hybrid maturity and evaluate interaction of corn flex hybrids and plant density under conditions of western Nebraska. Nine corn hybrids with relative maturity ranging from 86 to 105 days were sown between early May and late June in first and between late April and early …


Deciphering The Genetic Architecture Of Key Female Floral Traits For Hybrid Wheat Seed Production, Juan David Jimenez Dec 2022

Deciphering The Genetic Architecture Of Key Female Floral Traits For Hybrid Wheat Seed Production, Juan David Jimenez

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Wheat (Triticum aestivum L.) is a staple cereal that provides 20% of the calories and proteins in human intake (Ray et al., 2013). Global population is projected to increase to 9.7 billion by 2050. Food production must increase by 70% to feed this future population. Wheat production is in crisis due to political and environmental challenges and is projected to decline by 0.8% in 2022 (FAO, 2022). To ensure food security yield genetic gain must increase by around 1.4% annually. Taking advantage of heterosis, hybrid wheat has the potential to boost grain yield. However, hybrid wheat seed production systems …


Micronutrient Concentration Effects On Lettuce Growth And Susceptibility To Pythium, Kalyn M. Helms Dec 2022

Micronutrient Concentration Effects On Lettuce Growth And Susceptibility To Pythium, Kalyn M. Helms

Graduate Theses and Dissertations

In hydroponic production waterborne pathogens such as Pythium are ubiquitous and continually threaten a wide range of Controlled Environment Agriculture (CEA) crops in hydroponic production, including but not limited to: lettuce, spinach, basil, arugula, cucumber, tomato, sweet pepper, roses, chrysanthemums, and cannabis (Sutton et al., 2006; Gull, 2002; McGehee and Raudales, 2021; Gillespie, 2020). Despite extensive sanitation measures, disease control in hydroponics is fallible and requires constant surveillance and management to minimize outbreaks (Sutton et al., 2006). A potential disease suppression strategy is to increase micronutrient concentrations within hydroponic systems to naturally strengthen plant defenses against pathogens such as Pythium. …


Comparative Genomics Of Five Valsa Species Gives Insights On Their Pathogenicity Evolution, Guangchao Sun, Shichang Xie, Lin Tang, Chao Zhao, Mian Zhang, Lili Huang Dec 2022

Comparative Genomics Of Five Valsa Species Gives Insights On Their Pathogenicity Evolution, Guangchao Sun, Shichang Xie, Lin Tang, Chao Zhao, Mian Zhang, Lili Huang

Department of Agronomy and Horticulture: Faculty Publications

Valsa is a genus of ascomycetes within the Valsaceae family. This family includes many wood destructive pathogens such as the well known Valsa mali and Valsa pyri which cause canker diseases in fruit trees and threaten the global fruit production. Lack of genomic information of this family is impeding our understandings about their evolution and genetic basis of their pathogenicity divergence. Here, we report genome assemblies of Valsa malicola, Valsa persoonii, and Valsa sordida which represent close relatives of Valsa mali and Valsa pyri with different host preferences. Comparative genomics analysis revealed that segmental rearrangements, inversions, and translocations …


Image Analysis And Machine Learning In Agricultural Research, Xinzheng Chen Dec 2022

Image Analysis And Machine Learning In Agricultural Research, Xinzheng Chen

Doctor of Plant Health Program: Dissertations and Student Research

Agricultural research has been a focus for academia and industry to improve human well-being. Given the challenges in water scarcity, global warming, and increased prices of fertilizer, and fossil fuel, improving the efficiency of agricultural research has become even more critical. Data collection by humans presents several challenges including: 1) the subjectiveness and reproducibility when doing the visual evaluation, 2) safety when dealing with high toxicity chemicals or severe weather events, 3) mistakes cannot be avoided, and 4) low efficiency and speed.

Image analysis and machine learning are more versatile and advantageous in evaluating different plant characteristics, and this could …


Understanding The Influence Of Excessive Fertilization And Water Relations On Peach (Prunus Persica (L.) Batsch) Skin Bronzing, Sydney Lykins Dec 2022

Understanding The Influence Of Excessive Fertilization And Water Relations On Peach (Prunus Persica (L.) Batsch) Skin Bronzing, Sydney Lykins

All Theses

This project evaluated possible causes of peach skin bronzing, a brown-blotchy skin discoloration on mid to late-season peaches that causes drastic decreases to grower incomes in the Southeastern U.S. It is known that there is no bacterial, insectile, viral, or fungal cause of bronzing; thus, these studies focused on potential nutrient imbalances within the peach. On the ‘PF23’ variety, factors of irrigation, potassium fertilization, and transpiration-inhibiting sprays were tested to see the various effects on the incidence and severity of bronzing. The hypothesis was that excessive irrigation and excessive soil potassium concentrations would induce a higher incidence and severity of …


Comparative Genome Analysis Of Plant Ascomycete Fungal Pathogens With Different Lifestyles Reveals Distinctive Virulence Strategies, Yansu Wang, Jie Wu, Jiacheng Yan, Ming Guo, Lei Xu, Liping Hou, Quan Zou Dec 2022

Comparative Genome Analysis Of Plant Ascomycete Fungal Pathogens With Different Lifestyles Reveals Distinctive Virulence Strategies, Yansu Wang, Jie Wu, Jiacheng Yan, Ming Guo, Lei Xu, Liping Hou, Quan Zou

Department of Agronomy and Horticulture: Faculty Publications

Background: Pathogens have evolved diverse lifestyles and adopted pivotal new roles in both natural ecosystems and human environments. However, the molecular mechanisms underlying their adaptation to new lifestyles are obscure. Comparative genomics was adopted to determine distinct strategies of plant ascomycete fungal pathogens with different lifestyles and to elucidate their distinctive virulence strategies. Results: We found that plant ascomycete biotrophs exhibited lower gene gain and loss events and loss of CAZyme-encoding genes involved in plant cell wall degradation and biosynthesis gene clusters for the production of secondary metabolites in the genome. Comparison with the candidate effectome detected distinctive variations between …


Biobased Sprayable Mulch Films Suppressed Annual Weeds In Vegetable Crops, Eliott Gloeb, Sibel Irmak, Loren Isom, John L. Lindquist, Samuel E. Wortman Dec 2022

Biobased Sprayable Mulch Films Suppressed Annual Weeds In Vegetable Crops, Eliott Gloeb, Sibel Irmak, Loren Isom, John L. Lindquist, Samuel E. Wortman

Department of Agronomy and Horticulture: Faculty Publications

Biobased sprayable mulch (BSM) films are a potential alternative to herbicides, polyethylene plastic mulch film, and hand weeding for specialty crops. We developed a series of BSM films using locally available biomaterials [including corn (Zea mays) starch, glycerol, keratin hydrolysate, corn gluten meal, corn zein, eggshells, and isolated soy (Glycine max) protein] and tested their effects on weeds and crop yield during a total of seven greenhouse or field trials between 2017 and 2019 in Nebraska, USA. Application rates of BSM films applied in pots (greenhouse), planting holes in plastic film (field), or bed tops (field) …


Exploring Soil-Tree Interactions After Applying Organic Matter Amendments To Peach Orchards In The Subtropical Climate Of The Southeastern United States, Brian Lawrence Dec 2022

Exploring Soil-Tree Interactions After Applying Organic Matter Amendments To Peach Orchards In The Subtropical Climate Of The Southeastern United States, Brian Lawrence

All Dissertations

Soil disturbance from the cultivation and intensive management of orchards in hot and humid subtropical climates can reduce soil organic matter (OM). This research explored how the addition of OM amendments impact soil and tree parameters in two field studies and how proximity to OM may elicit unique genomic response of peach roots in a split-root pot study. The first field study applied two rates of compost (1x and 2x) and subsequently reduced fertilizer applications annually to a virgin and replant orchard location. While both compost rates successfully replaced fertilizers, only the 2x rate in the replant location increased soil …


In-Field And Greenhouse Assessments Of A Selection Of Preemergent Herbicides On Newly Planted Blackberries, Kayla Knepp Dec 2022

In-Field And Greenhouse Assessments Of A Selection Of Preemergent Herbicides On Newly Planted Blackberries, Kayla Knepp

Graduate Theses and Dissertations

This field trial assesses some of the preemergence herbicide options available to growers and their effect on newly transplanted blackberries (Rubus L. subgenus Rubus Watson). Weed control has recently been surveyed as a top priority for blackberry growers; however, limited preemergence herbicides are registered for new blackberry plantings. Weed control is an ongoing component of blackberry production and with few in-season postemergence herbicide options available, growers rely on preemergence herbicides to maintain clean fields. The preemergent herbicides assessed in this trial were chosen with the intention to broaden the chemical control options available to growers in new plantings. A two-year …