Horticulture Commons^™

Optimizing The Use Of A Liquid Handling Robot To Conduct A High Throughput Forward Chemical Genetics Screen Of Arabidopsis Thaliana, B. Kirtley Amos, Victoria G. Pook, Seth Debolt

Horticulture Faculty Publications

Chemical genetics is increasingly being employed to decode traits in plants that may be recalcitrant to traditional genetics due to gene redundancy or lethality. However, the probability of a synthetic small molecule being bioactive is low; therefore, thousands of molecules must be tested in order to find those of interest. Liquid handling robotics systems are designed to handle large numbers of samples, increasing the speed with which a chemical library can be screened in addition to minimizing/standardizing error. To achieve a high-throughput forward chemical genetics screen of a library of 50,000 small molecules on Arabidopsis thaliana (Arabidopsis), protocols using a …

Kelch F-Box Protein Positively Influences Arabidopsis Seed Germination By Targeting Phytochrome-Interacting Factor1, Manoj Majee, Santosh Kumar, Praveen Kumar Kathare, Shuiqin Wu, Derek Gingerich, Nihar R. Nayak, Louai Salaita, Randy Dinkins, Kathleen Martin, Michael M. Goodin, Lynnette M. A. Dirk, Taylor D. Lloyd, Ling Zhu, Joseph Chappell, Arthur G. Hunt, Richard Vierstra, Enamul Huq, A. Bruce Downie

Horticulture Faculty Publications

Seeds employ sensory systems that assess various environmental cues over time to maximize the successful transition from embryo to seedling. Here we show that the Arabidopsis F-BOX protein COLD TEMPERATURE-GERMINATING (CTG)-10, identified by activation tagging, is a positive regulator of this process. When overexpressed (OE), CTG10 hastens aspects of seed germination. CTG10 is expressed predominantly in the hypocotyl, and the protein is localized to the nucleus. CTG10 interacts with PHYTOCHROME-INTERACTING FACTOR 1 (PIF1) and helps regulate its abundance in planta. CTG10-OE accelerates the loss of PIF1 in light, increasing germination efficiency, while PIF1-OE lines fail to complete germination in …

Keragaan Tiga Jenis Planlet Anggrek Phalaenopsis Asal Protocorm Yang Diinduksi Ethyl Methyl Sulfonate (Ems) Secara In Vitro, Romiyadi, Ai Komariah, Suseno Amien

Jurnal Kultivasi

This research was conducted to determine the effect of concentration of Ethyl Methyl Sulfonate (EMS) to performance of three types of Phalaenopsis Planlets and to know the optimum concentration of EMS on any type of Phalaenopsis orchids. The experiment was conduc-ted at Tissue Culture Laboratory of the Faculty of Agriculture, Winaya Mukti University, Sumedang. It used a completely randomized design (CRD) with factorial pattern, that consisted of two factors and replicated twice.The first factor was the type of Phalaenopsis that resulted form hybridization which consisted of v1 (Phalaenopsis 717 X Phalae-nopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), and …

Effect Of Cultivar And Type On Pepper Yield And Weight, Elizabeth 'Egan' Blessinger

Masters Theses & Specialist Projects

Peppers (Capsicum) are a valuable commodity throughout the world. They provide food, coloring additives, vitamins, and ornamental aesthetics. Peppers can be grown in many different areas of the world and their success is based on variety selection. Breeding cultivars for superior performance is critical to success. Recent consumer desire for less chemical use throughout the growing process has resulted in new cultivar developments..

The objective of this research was to investigate how cultivar and type affect the yield and weight of field grown peppers. This study provides valuable information for growers in determining which cultivars are suitable for production in …

Comparison Of Three Assembly Strategies For A Heterozygous Seedless Grapevine Genome Assembly, Sagar Patal, Zhixiu Lu, Xiaozhu Jin, Padmapriya Swaminathan, Erliang Zeng, Anne Y. Fennell Dr

Agronomy, Horticulture and Plant Science Faculty Publications

Background
De novo heterozygous assembly is an ongoing challenge requiring improved assembly approaches. In this study, three strategies were used to develop de novo Vitis vinifera ‘Sultanina’ genome assemblies for comparison with the inbred V. vinifera (PN40024 12X.v2) reference genome and a published Sultanina ALLPATHS-LG assembly (AP). The strategies were: 1) a default PLATANUS assembly (PLAT_d) for direct comparison with AP assembly, 2) an iterative merging strategy using METASSEMBLER to combine PLAT_d and AP assemblies (MERGE) and 3) PLATANUS parameter modifications plus GapCloser (PLAT*_GC).
Results
The three new assemblies were greater in size than the AP assembly. PLAT*_GC had the …

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

This paper reports on six bicolor sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

This paper reports on fifteen bicolor, two yellow, and two white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Midwest Vegetable Trial Report For 2017, Elizabeth Maynard, Brad R. Bergefurd, Wenjing Guan, Petrus Langenhoven

Purdue Fruit and Vegetable Research Reports

This is a compilation of 19 research trial reports from six land-grant universities in the Midwestern United States. Crops include green beans, cantaloupe, cucumber, ornamental corn, pepper, potato, pumpkin, butternut squash, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed specific growing practices: plant spacing for sweet corn.

Tomato Cultivar Evaluation In High Tunnels, Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

Tomatoes are a common crop grown in unheated greenhouses, commonly called high tunnels. Production in these structures has increased in recent years. This trial was conducted to evaluate varieties for their performance as a summer-harvested crop in soil-based high tunnel production systems. Five hybrid determinate varieties or line, one hybrid indeterminate, and one open-pollinated indeterminate variety were compared for yield, earliness, fruit size, and quality.

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

This paper reports on six bicolor sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Tomato Cultivar Evaluation In High Tunnels, Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

Tomatoes are a common crop grown in unheated greenhouses, commonly called high tunnels. Production in these structures has increased in recent years. This trial was conducted to evaluate varieties for their performance as a summer-harvested crop in soil-based high tunnel production systems. Five hybrid determinate varieties or line, one hybrid indeterminate, and one open-pollinated indeterminate variety were compared for yield, earliness, fruit size, and quality.

Pumpkin Variety Performance With And Without Treatment For Powdery Mildew In Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Purdue Fruit and Vegetable Research Reports

Pumpkins are grown on 5,000 acres in Indiana with an average yield of 6.75 tons per acre. Successful pumpkin production requires the use of varieties that yield well and produce pumpkins of the size, shape, color, and quality demanded by the market. Genetic resistance to the fungal disease powdery mildew is present in some varieties. This trial was designed to evaluate performance of pumpkin varieties in northern Indiana with and without treatment for powdery mildew. The trial included fourteen orange jack-o-lantern size pumpkins, one small or 'pie' pumpkin and one specialty yellow pumpkin.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

This paper reports on fifteen bicolor, two yellow, and two white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Pumpkin Variety Performance With And Without Treatment For Powdery Mildew In Northern Indiana, 2017, Elizabeth Maynard, Erin A. Bluhm

Midwest Vegetable Trial Reports

Pumpkins are grown on 5,000 acres in Indiana with an average yield of 6.75 tons per acre. Successful pumpkin production requires the use of varieties that yield well and produce pumpkins of the size, shape, color, and quality demanded by the market. Genetic resistance to the fungal disease powdery mildew is present in some varieties. This trial was designed to evaluate performance of pumpkin varieties in northern Indiana with and without treatment for powdery mildew. The trial included fourteen orange jack-o-lantern size pumpkins, one small or 'pie' pumpkin and one specialty yellow pumpkin.

Midwest Vegetable Trial Report For 2017, Elizabeth Maynard, Brad R. Bergefurd, Wenjing Guan, Petrus Langenhoven

Midwest Vegetable Trial Reports

This is a compilation of 19 research trial reports from six land-grant universities in the Midwestern United States. Crops include green beans, cantaloupe, cucumber, ornamental corn, pepper, potato, pumpkin, butternut squash, sweet corn, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed specific growing practices: plant spacing for sweet corn.

Reciprocal Effect Of Parental Lines On The Physiological Potential And Seed Composition Of Corn Hybrid Seeds, Juliana F. Santos, Lynnette M. A. Dirk, A. Bruce Downie, Mauricio F. G. Sanches, Roberval D. Vieira

Horticulture Faculty Publications

Obtaining corn hybrid seeds (Zea mays L.) with high vigour depends on the parental lines and the direction of the cross, and this relates to seed desiccation tolerance and composition. This research studied reciprocal crosses between pairs of proprietary, elite parent lines (L1 and L5; L2 and L4) producing hybrid seeds with different qualities attempting to correlate vigour with seed composition, focusing on storage proteins, starch and soluble sugar amounts. Four corn hybrid seed lots produced from reciprocal crosses were compared (HS 15 with HS 51, and HS 24 with HS 42) by assessing germination, vigour, and seedling emergence …

Phenotypic Variation And Genetic Purity Of The Original 'Prime-Jim®' X 'Arapaho' Population, Loren M. Luther

Horticulture Undergraduate Honors Theses

In 2013, Castro et al., produced the first linkage map for primocane-fruiting blackberries using the cross ‘Prime-Jim®’ x ‘Arapaho.’ This mapping population has been maintained since the original experiment with the hopes of conducting future studies. Further research was determined to be needed on the population to better characterize additional phenotypic traits, and since nine years had passed since the seedlings were established, there was a need to assess the continued genetic purity of the population using molecular methods. Phenotypic data was collected to analyze the variation of the population including soluble solids content, titratable acidity, average weight, shape, and …

The Lx Report Of The Bean Improvement Cooperative No. 60, March 2017.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

CONTENTS

LX Annual Report of the Bean Improvement Cooperative xi

BIC Coordinating Committee Membership - 1957 to 2017 . xii

BIC Meritorious Service and Distinguished Achievement Award Recipients - 1957 to 2015 xiii

BIC Awards Nomination Request for 2017 xvi

BIC/NAPIA Meeting in 2017 . xix

In Memory of Guillermo E. Galvez-Enriquez xx

RESEARCH PAPERS FOR 2017

OPTIMIZING SPORULATION OF Pseudocercospora griseola IN VITRO • Paula F. de Pádua1, Rafael Pereira1, Luanna B. W. Gomes1 and Elaine A. de Souza1* 1

FINE MAPPING THE BROAD SPECTRUM ANTHRACNOSE RESISTANCE GENE IN AMENDOIM CAVALO 3 • Gilio, T.A.S.1, Oscar P. Hurtado-Gonzales2, Giseli …

Midwest Vegetable Trial Report For 2016, Elizabeth Maynard, Brad Bergefurd

Purdue Fruit and Vegetable Research Reports

This is a compilation of 21 research trial reports from six land-grant universities in the midwestern United States. Crops include dry beans, cantaloupe, cucumber, pepper, pumpkin, sweet corn, summer squash, winter squash, strawberry, tomato, watermelon, and winter melon. Several crops were evaluated in high tunnels or hoop houses. Most trials evaluated different cultivars or varieties. Three reports addressed specific growing practices: the use of poly-coated urea to supply nitrogen to sweet corn, potassium fertilization for high tunnel tomatoes, and use of cereal rye cover crop in butternut squash.

Midwest Vegetable Trial Report For 2016, Elizabeth Maynard, Brad Bergefurd

Midwest Vegetable Trial Reports

This is a compilation of 21 research trial reports from six land-grant universities in the midwestern United States. Crops include dry beans, cantaloupe, cucumber, pepper, pumpkin, sweet corn, summer squash, winter squash, strawberry, tomato, watermelon, and winter melon. Several crops were evaluated in high tunnels or hoop houses. Most trials evaluated different cultivars or varieties. Three reports addressed specific growing practices: the use of poly-coated urea to supply nitrogen to sweet corn, potassium fertilization for high tunnel tomatoes, and use of cereal rye cover crop in butternut squash.

Screening Strawberry Clones For Anthracnose Disease Resistance Using Traditional Techniques And Molecular Markers, Melinda A. Miller-Butler

Dissertations

Cultivated strawberry, Fragaria x ananassa Duchesne, is host for many pathogens. One of the most destructive diseases of strawberry is anthracnose, whose symptoms include fruit rot, leaf and petiole lesions, crown rot, wilt, and death. Three species of Colletotrichum are considered causative agents of anthracnose diseases of strawberry. Colletotrichum acutatum causes anthracnose fruit rot, has a broad host range, and occurs in most areas of the world where strawberries are grown. Colletotrichum fragariae, the primary causal fungus of anthracnose crown rot, may infect all aboveground parts of the strawberry plant, and has restricted host and geographic ranges. Colletotrichum gloeosporioides …

Toward The Elucidation Of Cytoplasmic Diversity In North American Grape Breeding Programs, Jonathan Fresnedo-Ramirez, Qi Sun, Chin-Feng Hwang, Craig A. Ledbetter, David W. Ramming, Anne Fennell, M. Andrew Walker, James J. Luby, Matthew D. Clark, Jason P. Londo, Lance Cadle-Davidson, Gan-Yuan Zhong

Agronomy, Horticulture and Plant Science Faculty Publications

Plants have an intriguing tripartite genetic system: Nuclear genome 9 Mitochondria 9 Plastids and their interactions may impact germplasm breeding. In grapevine, the study of cytoplasmic genomes has been limited, and their role with respect to grapevine germplasm diversity has yet to be elucidated. In the present study, the results of an analysis of the cytoplasmic diversity among 6073 individuals (comprising cultivars, interspecific hybrids and segregating progenies) are presented. Genotyping by sequencing (GBS) was used to elucidate plastid and mitochondrial DNA sequences, and results were analyzed using multivariate techniques. Single nucleotide polymorphism (SNP) effects were annotated in reference to plastid …

Midwest Vegetable Trial Report For 2015, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

This is a compilation of 19 research trial reports from six land-grant universities in the midwestern United States. Crops include bok choy, cantaloupe, cucumber, pepper, pumpkin, sweet corn, squash, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties, including one investigating cantaloupe variety influence on cucumber beetle presence and incidence of bacterial wilt. Four reports addressed specific growing practices: the use of poly-coated urea to supply nitrogen to sweet corn, biochar use in a high tunnel bag culture system, use of cereal rye cover crop in butternut squash, and pollinizer …

Mini- And Heirloom Sweet Pepper Variety Performance In High Tunnels, 2015, Elizabeth Maynard, Israel S. Calsoyas

Purdue Fruit and Vegetable Research Reports

Trials on mini sweet peppers and other specialty sweet peppers in the Midwest have not recently been reported. We undertook this project to compare yield and fruit characteristics of hybrid and heirloom peppers grown in high tunnels using organic and conventional production methods.

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas

Purdue Fruit and Vegetable Research Reports

This paper reports on seven bicolor and two yellow sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Midwest Vegetable Trial Report For 2015, Elizabeth Maynard

Midwest Vegetable Trial Reports

This is a compilation of 19 research trial reports from six land-grant universities in the midwestern United States. Crops include bok choy, cantaloupe, cucumber, pepper, pumpkin, sweet corn, squash, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties, including one investigating cantaloupe variety influence on cucumber beetle presence and incidence of bacterial wilt. Four reports addressed specific growing practices: the use of poly-coated urea to supply nitrogen to sweet corn, biochar use in a high tunnel bag culture system, use of cereal rye cover crop in butternut squash, and pollinizer …

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas

Midwest Vegetable Trial Reports

This paper reports on thirteen bicolor, two yellow, and three white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Mini- And Heirloom Sweet Pepper Variety Performance In High Tunnels, 2015, Elizabeth Maynard, Israel S. Calsoyas

Midwest Vegetable Trial Reports

Trials on mini sweet peppers and other specialty sweet peppers in the Midwest have not recently been reported. We undertook this project to compare yield and fruit characteristics of hybrid and heirloom peppers grown in high tunnels using organic and conventional production methods.

Supersweet Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas

Purdue Fruit and Vegetable Research Reports

This paper reports on thirteen bicolor, two yellow, and three white supersweet sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas

Midwest Vegetable Trial Reports

This paper reports on seven bicolor and two yellow sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.