Horticulture Commons^™

Genetic Control Of Morphometric Diversity In The Maize Shoot Apical Meristem, Samuel Leiboff, Xianran Li, Heng-Cheng Hu, Natalie Todt, Jinliang Yang, Xiao Li, Xiaoqing Yu, Gary J. Muehlbauer, Marja C.P. Timmermans, Jianming Yu, Patrick S. Schnable, Michael J. Scanlon

Department of Agronomy and Horticulture: Faculty Publications

The maize shoot apical meristem (SAM) comprises a small pool of stem cells that generate all above-ground organs. Although mutational studies have identified genetic networks regulating SAM function, little is known about SAM morphological variation in natural populations. Here we report the use of high-throughput image processing to capture rich SAM size variation within a diverse maize inbred panel. We demonstrate correlations between seedling SAM size and agronomically important adult traits such as flowering time, stem size and leaf node number. Combining SAM phenotypes with 1.2 million single nucleotide polymorphisms (SNPs) via genome-wide association study reveals unexpected SAM morphology candidate …

Differential Expression Analysis In Rna-Seq By A Naive Bayes Classifier With Local Normalization, Yongchao Dou, Xiaomei Guo, Lingling Yuan, David R. Holding, Chi Zhang

Department of Agronomy and Horticulture: Faculty Publications

To improve the applicability of RNA-seq technology, a large number of RNA-seq data analysis methods and correction algorithms have been developed. Although these new methods and algorithms have steadily improved transcriptome analysis, greater prediction accuracy is needed to better guide experimental designs with computational results. In this study, a new tool for the identification of differentially expressed genes with RNA-seq data, named GExposer, was developed. This tool introduces a local normalization algorithm to reduce the bias of nonrandomly positioned read depth. The naive Bayes classifier is employed to integrate fold change, transcript length, and GC content to identify differentially expressed …

Soil And Crop Response To Stover Removal From Rainfed And Irrigated Corn, Ian Kenney, Humberto Blanco-Canqui, Deann R. Presley, Charles W. Rice, Keith Janssen, Brian Olson

Department of Agronomy and Horticulture: Faculty Publications

Excessive corn (Zea mays L.) stover removal for biofuel and other uses may adversely impact soil and crop production. We assessed the effects of stover removal at 0, 25, 50, 75, and 100% from continuous corn on water erosion, corn yield, and related soil properties during a 3-year study under irrigated and no-tillage management practice on a Ulysses silt loam at Colby, irrigated and strip till management practice on a Hugoton loam at Hugoton, and rainfed and no-tillage management practice on a Woodson silt loam at Ottawa in Kansas, USA. The slope of each soil was <1%. One year after removal, complete (100%) stover removal resulted in increased losses of sediment by 0.36–0.47 Mg ha-1 at the irrigated sites, but, at the rainfed site, removal at rates as low as 50% resulted in increased sediment loss by 0.30 Mg ha-1 and sediment-associated carbon (C) by 0.29 kg ha-1. Complete stover removal reduced wet aggregate stability of the soil at the irrigated sites in the first year after removal, but, at the rainfed site, wet aggregate stability was reduced in all years. Stover removal at rates ≥ 50%resulted in reduced soil water content, increased soil temperature in summer by 3.5–6.8 °C, and reduced tem-perature in winter by about 0.5 °C. Soil C pool tended to decrease and crop yields tended to increase with an increase in stover removal, but 3 years after removal, differences were not significant. Overall, stover removal at rates ≥50% may enhance grain yield but may increase risks of water erosion and negatively affect soil water and temperature regimes in this region.

High Tunnel Design, Site Development, And Construction, Stacy A. Adams, Kim Todd

Department of Agronomy and Horticulture: Faculty Publications

High tunnels are low technology plant growing structures that provide opportunity for season extension, severe weather protection, and modified environments to increase productivity and visual quality of harvested plant products. This publication describes high tunnel components, site selection, development, covering materials, installation, and operation.

Experienced specialty plant growers can increase the quality of their products and the duration of their marketing of high- value crops through the use of high tunnels. High tunnels give growers the opportunity to plant earlier and include more sequential planting dates. This can result in early- season, high- dollar returns and the ability to offer …

Dispersal: A Multidisciplinary Investigation Of Plant Life, Alexandra E. Arzt

Theses and Dissertations

Using plants as a basis for exploring the interstices between the human and nonhuman, this thesis investigates ideas of awareness, intelligence, deep time, animism, and the fluctuating human perception of the agency of Nature. It outlines environmental art practices since the 1950s involving vegetal life. In addition, the paper provides a critical analysis of plant perception of Jakob von Uexküll’s work and theories of vital materialism and “critical plant studies” while noting recent studies in plant neurobiology. In my work, plants become active participants via their movement, seeding, and smell. This study takes the form of imitation, purposeful symbiosis, anthropomorphism, …

Metabolic Engineering Of Oilseed Crops To Produce High Levels Of Novel Acetyl Glyceride Oils With Reduced Viscosity, Freezing Point And Calorific Value, Jinjie Liu, Adam Rice, Kathleen Mcglew, Vincent Shaw, Hyunwoo Park, Thomas E. Clemente, Mike Pollard, John Ohlrogge, Timothy P. Durrett

Department of Agronomy and Horticulture: Faculty Publications

Seed oils have proved recalcitrant to modification for the production of industrially useful lipids. Here, we demonstrate the successful metabolic engineering and subsequent field production of an oilseed crop with the highest accumulation of unusual oil achieved so far in transgenic plants. Previously, expression of the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene in wild-type Arabidopsis seeds resulted in the accumulation of 45 mol% of unusual 3-acetyl-1,2- diacyl-sn-glycerols (acetyl-TAGs) in the seed oil (Durrett et al., 2010 PNAS 107:9464). Expression of EaDAcT in dgat1 mutants compromised in their ability to synthesize regular triacylglycerols increased acetyl-TAGs to 65 mol%. Camelina and soybean …

Functional Genomics And Microbiome Profiling Of The Asian Longhorned Beetle (Anoplophora Glabripennis) Reveal Insights Into The Digestive Physiology And Nutritional Ecology Of Wood Feeding Beetles, Erin D. Scully, Scott M. Geib, John E. Carlson, Ming Tien, Duane Mckenna, Kelli Hoover

Department of Agronomy and Horticulture: Faculty Publications

Background: Wood-feeding beetles harbor an ecologically rich and taxonomically diverse assemblage of gut microbes that appear to promote survival in woody tissue, which is devoid of nitrogen and essential nutrients. Nevertheless, the contributions of these apparent symbionts to digestive physiology and nutritional ecology remain uncharacterized in most beetle lineages.

Results: Through parallel transcriptome profiling of beetle- and microbial- derived mRNAs, we demonstrate that the midgut microbiome of the Asian longhorned beetle (Anoplophora glabripennis), a member of the beetle family Cerambycidae, is enriched in biosynthetic pathways for the synthesis of essential amino acids, vitamins, and sterols. Consequently, the midgut …

Comprehensive Characterization And Rna-Seq Profiling Of The Hd-Zip Transcription Factor Family In Soybean (Glycine Max) During Dehydration And Salt Stress, Vikas Belamkar, Nathan T. Weeks, Arvind K. Bharti, Andrew Farmer, Michelle A. Graham, Steven B. Cannon

Department of Agronomy and Horticulture: Faculty Publications

Background:

The homeodomain leucine zipper (HD-Zip) transcription factor family is one of the largest plant specific superfamilies, and includes genes with roles in modulation of plant growth and response to environmental stresses. Many HD-Zip genes are characterized in Arabidopsis (Arabidopsis thaliana), and members of the family are being investigated for abiotic stress responses in rice (Oryza sativa), maize (Zea mays), poplar (Populus trichocarpa) and cucumber (Cucmis sativus). Findings in these species suggest HD-Zip genes as high priority candidates for crop improvement.

Results:

In this study we have identified members of …

Nebline, November/December 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Tiny Tastes Can Total BIG CALORIES Over the Holiday Season

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Nebline, October 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Smart Snacking

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Damping-Off, Claudia Nischwitz

All Current Publications

No abstract provided.

Nebline, September 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Youth Showcase Their Achievements at Super Fair

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Cold Frames, High Tunnels, And Greenhouses: Choose A Growing Structure Best For You, Stacy A. Adams, Kim A. Todd

Department of Agronomy and Horticulture: Faculty Publications

Cold frames, high tunnels, and greenhouses are plant growth structures that can be used to extend the growing season or allow for use year around for growing high value specialty crops. This publication discusses the various attributes and applications of each so that plant producers in teaching, research, extension, commercially or as a hobby can determine the best structure to meet individual needs.

Cold frames, high tunnels, and greenhouses are structures that provide hobbyists and growers with options for plant protection and season extension. They vary in cost, size, structural complexity, and ability for year-round usage. The small cold frame …

Nebline, August 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Cherry Creek Habitat Provides Opportunities for Pollinator Education

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Special pullout section: Lancaster County Super Fair 2014 Schedule & Map

Effects Of Culture Media And Plant Growth Regulators On Micropropagation Of Willow (Salix Matsudana ‘Golden Spiral’) And Hazelnut (Corylus Colurna ‘Te Terra Red), Dongxue Shi

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The branches and leaves of Salix matsudana ‘Golden Spiral’ , willow, grow in a twisted manner, which makes it an important bonsai plant. Its mature stems have potential for the woody cut floral industry. Corylus colurna ‘ Te Terra Red’, hazelnut, distinguished by red or purple leaves in the spring, has potential ornamental value in the horticulture industry for landscape use. Micropropagation of these two plants could provide more and healthier plantlets for rapid commercial scale-up by the nursery industry. Nas and Read Medium (NRM) is a newer medium developed specifically for hazelnut species by Nas and Read based on …

Nebline, July 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Table Setting Contest is a Fun Way to Learn Nutrition, Setting a Table, Presenting and More!

Food & Health

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Farm & Acreage

Home & Family Living

Horticulture

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Nebline, June 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Nurturing Unmarried Parents and Their Children

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Grazing Method Effects On Forage Production, Utilization, And Animal Performance On Nebraska Sandhills Meadow, Miles D. Redden

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Mob grazing using ultrahigh stocking densities is promoted as a tool to increase the health and productivity of grasslands by increasing nutrient cycling and soil organic matter. Mob grazing can be defined as a strategy in which area available to grazing animals is restricted to achieve stocking densities of 200,000 kg/ha or greater. Objectives were to determine herbage production, utilization, and cattle weight gains among ultrahigh stocking density grazing and more conventional grazing methods on a Sandhills subirrigated meadow. Treatments included two replications of each of the following: four-pasture rotational grazing with two occupations per pasture in an 80-day grazing …

Synthesis Of The Effect Of Fire On Lesser Prairie‐Chickens, Eric Thacker, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

In this synthesis, we reviewed the scientific literature pertaining to the effects of fire on lesser prairie‐chickens and their habitat. Overall, research is generally lacking that directly assesses relationships among fire, both as a regime and a discrete event, to its effect on lesser prairie‐chicken behavior and habitat value. Yet, research has quantified the effects of fire on vegetation throughout the distribution of the lesser prairie‐chicken, which has been linked to known metrics of habitat value and broad landscape‐level habitat requirements.

To summarize the literature, lesser prairie‐chickens require broad landscapes of fire‐dependent grass‐shrub vegetation. Changes in the eco‐evolutionary fire regime …

Nebline, May 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: The Cost of Invasive Species

Food & Health

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Farm & Acreage

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Pests & Wildlife

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Stacking Of A Stearoyl-Acp Thioesterase With A Dual-Silenced Palmitoyl-Acp Thioesterase And Δ12 Fatty Acid Desaturase In Transgenic Soybean, Hyunwoo Park, George L. Graef, Yixiang Xu, Patrick Tenopir, Thomas E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max (L.) Merr) is valued for both its protein and oil, whose seed is composed of 40% and 20% of each component, respectively. Given its high percentage of polyunsaturated fatty acids, linoleic acid and linolenic acid, soybean oil oxidative stability is relatively poor. Historically food processors have employed a partial hydrogenation process to soybean oil as a means to improve both the oxidative stability and functionality in end-use applications. However, the hydrogenation process leads to the formation of trans-fats, which are associated with negative cardiovascular health. As a means to circumvent the need for the hydrogenation process, genetic …

Overview Of The Integrated Pest Management (Ipm) Terrain And Activities In Furtherance Of The Walmart Initiative To Purchase Fresh Vegetables From Local Growers In Alabama, Franklin Quarcoo, Conrad Bonsi

Professional Agricultural Workers Journal

Tuskegee University has been providing various types of technical expertise to limited resource farmers who have been supplying Walmart with collard greens, watermelons and purple hull peas. A number of pests bedevil the cultivation of these crops; cost-effective management methods for these pests are needed. The objectives of this paper are to document the IPM activities associated with supplying produce to Walmart; summarize pest problems encountered on the afore-stated crops; recommend IPM methods suitable for limited resource farmers; and suggest other activities that ensure that farmers incur even less pest-related crop losses. Anthracnose was the major pest encountered on watermelons. …

Nebline, April 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Backyard Farmer -- Six Decades of Growing Gardeners and Protecting the Environment

Food & Health

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Special Pullout Section: Weed Awareness

Nebline, March 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Make a Difference -- Reduce, Reuse, Recycle

Food & Health

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Nebline, February 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Garbology Uses Science to Teach Environmental Responsibility

Food & Health

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First Report Of Curvularia Inaequalis And Bipolaris Spicifera Causing Leaf Blight Of Buffalograss In Nebraska, B. S. Amaradasa, K. Amundsen

Department of Agronomy and Horticulture: Faculty Publications

During the summer of 2011, foliar blight was observed on buffalograss (Buchloë dactyloides (Nutt.) Engelm.) lawns in Lincoln and Waverly, Nebraska. Disease symptoms were common when buffalograss was growing above 30°C and in drought conditions. Disease symptoms began as dark brown oblong leaf spots, followed by leaf tip dieback and eventual blighting of entire tillers. Leaf infections would progress into patches of thinning turf. Diseased leaf pieces were rinsed in distilled water and placed on 1.5% water agar. Two mitosporic fungal species having conidial morphology of Curvularia and Bipolaris were isolated. Colonies of Curvularia isolates grown on ¼× PDA …

Nebline, January 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Youth Serve Their Community By Volunteering

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

Family and Community Education (FCE) Clubs

and other extension news and events

Quantitative Trait Locus Analysis Of Unsaturated Fatty Acids In A Recombinant Inbred Population Of Soybean, Xianzhi Wang, Guo-Liang Jiang, Marci Green, Roy A. Scott, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is an important oilseed crop which produces about 30 %of the world’s edible vegetable oil. The quality of soybean oil is determined by its fatty acid composition. Soybean oil high in oleic and low in linolenic fatty acids is desirable for human consumption and other uses. The objectives of this study were to identify quantitative trait loci (QTLs) for unsaturated fatty acids and to evaluate the genetic effects of single QTL and QTL combinations in soybean. A population of recombinant inbred lines derived from the cross of SD02-4-59 X A02-381100 was evaluated for fatty …

Detection And Confirmation Of Quantitative Trait Loci For Soybean Seed Isoflavones, Christopher J. Smallwood, Catherine N. Nyinyi, Dean A. Kopsell, Carl E. Sams, Dennis R. West, Pengyin Chen, Stella K. Kantartzi, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Interest in soybean [Glycine max (L.) Merr.] isoflavones has increased in recent years owing to numerous reported health benefits. Consequently, quantitative trait loci (QTL) detection for marker-assisted breeding for isoflavones is being examined for genetic gains. This study sought to detect QTL for soybean isoflavones in a population of 274 recombinant inbred lines derived from a cross between ‘Essex’ and ‘Williams 82’ that were subdivided and tested by maturity (early, mid, and late). The field tests were conducted in three environments in 2009 (Knoxville, TN; Harrisburg, IL; and Stuttgart, AR). The population was genotyped with 480 polymorphic single nucleotide …

Transcriptomic And Physiological Characterization Of The Fefe Mutant Of Melon (Cucumis Melo) Reveals New Aspects Of Iron-Copper Crosstalk, Brian M. Waters, Samuel A. Mcinturf, Keenan Amundsen

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) and copper (Cu) homeostasis are tightly linked across biology. In previous work, Fe deficiency interacted with Cu-regulated genes and stimulated Cu accumulation. The C940-fe (fefe) Fe-uptake mutant of melon (Cucumis melo) was characterized, and the fefe mutant was used to test whether Cu deficiency could stimulate Fe uptake. Wild-type and fefe mutant transcriptomes were determined by RNA-seq under Fe and Cu deficiency. FeFe-regulated genes included core Fe uptake, metal homeostasis, and transcription factor genes. Numerous genes were regulated by both Fe and Cu. The fefe mutant was rescued by high Fe or by …