Botany Commons^™

Domestication Reshaped The Genetic Basis Of Inbreeding Depression In A Maize Landrace Compared To Its Wild Relative, Teosinte, Luis Fernando Samayoa, Bode A. Olukolu, Chin Jian Yang, Qiuyue Chen, Markus G. Stetter, Alessandra M. York, Jose De Jesus Sanchez-Gonzalez, Jeffrey C. Glaubitz, Peter J. Bradbury, Maria Cinta Romay, Qi Sun, Jinliang Yang, Jeffrey Ross-Ibarra, Edward S. Buckler, John F. Doebley, James B. Holland

Department of Agronomy and Horticulture: Faculty Publications

Inbreeding depression is the reduction in fitness and vigor resulting from mating of close relatives observed in many plant and animal species. The extent to which the genetic load of mutations contributing to inbreeding depression is due to large-effect mutations versus variants with very small individual effects is unknown and may be affected by population history. We compared the effects of outcrossing and self-fertilization on 18 traits in a landrace population of maize, which underwent a population bottleneck during domestication, and a neighboring population of its wild relative teosinte. Inbreeding depression was greater in maize than teosinte for 15 of …

Identification Of Candidate Genes And Genomic Regions Associated With Adult Plant Resistance To Stripe Rust In Spring Wheat, Amira M. I. Mourad, Mohamed A. Abou-Zeid, Shamseldeen Eltaher, P. Stephen Baenziger, Andreas Börner

Department of Agronomy and Horticulture: Faculty Publications

Wheat stripe rust (caused by Puccinia striiformis f. sp. tritici) is a major disease that damages wheat plants and affects wheat yield all over the world. In recent years, stripe rust became a major problem that affects wheat yield in Egypt. New races appeared and caused breakdowns in the resistant genotypes. To improve resistance in the Egyptian genotypes, new sources of resistance are urgently needed. In the recent research, a set of 95 wheat genotypes collected from 19 countries, including Egypt, were evaluated for their resistance against the Egyptian race(s) of stripe rust under field conditions in the two …

Relationships Among College-Level Science Course Enrollment, Environmental Perception, And Pro-Environmental Attitude: Evidence From The Us General Social Survey, Mazbahul G. Ahamad, Fahian Tanin

Department of Agronomy and Horticulture: Faculty Publications

Understanding pro-environmental attitudes is critical to encouraging pollution-minimizing behaviors. Therefore, identifying associated factors is essential for understanding different types of pro-environmental attitudes. We aimed to investigate the associations among individuals’ college-level science course enrollment and their perceptions of the level of spending to improve and protect the environment, as well as their pro-environmental attitudes. We used nationwide population-based cross-sectional survey data from 2,348 individuals obtained from the General Social Survey in the United States. An ordered logistic model was used to examine the associations among college-level science course enrollment, environmental perception, and pro-environmental attitude. We found that science course enrollment …

Cold Conditioned: Discovery Of Novel Alleles For Low-Temperature Tolerance In The Vavilov Barley Collection, Ahmad H. Sallam, Kevin P. Smith, Gongshe Hu, Jamie Sherman, Peter Stephen Baeziger, Jochum Wiersma, Carl Duley, Eric J. Stockinger, Mark E. Sorrells, Tamas Szinyei, Igor G. Loskutov, Olga N. Kovaleva, Jed Eberly, Brian J. Steffenson

Department of Agronomy and Horticulture: Faculty Publications

Climate changes leading to higher summer temperatures can adversely affect cool season crops like spring barley. In the Upper Midwest region of the United States, one option for escaping this stress factor is to plant winter or facultative type cultivars in the autumn and then harvest in early summer before the onset of high-temperature stress. However, the major challenge in breeding such cultivars is incorporating sufficient winter hardiness to survive the extremely low temperatures that commonly occur in this production region. To broaden the genetic base for winter hardiness in the University of Minnesota breeding program, 2,214 accessions from the …

Sustainable Intensification For A Larger Global Rice Bowl, Shen Yuan, Bruce A. Linquist, Lloyd T. Wilson, Kenneth G. Cassman, Alexander M. Stuart, Valerien Pede, Berta Miro, Kazuki Saito, Nurwulan Agustiani, Vina Eka Aristya, Leonardus Y. Krisnadi, Alencar Junior Zanon, Alexandre Bryan Heinemann, Gonzalo Carracelas, Nataraja Subash, Pathula S. Brahmanand, Tao Li, Shaobing Peng, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Future rice systems must produce more grain while minimizing the negative environmental impacts. A key question is how to orient agricultural research & development (R&D) programs at national to global scales to maximize the return on investment. Here we assess yield gap and resource-use efficiency (including water, pesticides, nitrogen, labor, energy, and associated global warming potential) across 32 rice cropping systems covering half of global rice harvested area. We show that achieving high yields and high resource-use efficiencies are not conflicting goals. Most cropping systems have room for increasing yield, resource-use efficiency, or both. In aggregate, current total rice production …

Climate And Agronomy, Not Genetics, Underpin Recent Maize Yield Gains In Favorable Environments, Gonzalo Rizzo, Juan Pablo Monzon, Fatima Amor Tenorio, Réka Howard, Kenneth G. Cassman, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Quantitative understanding of factors driving yield increases of major food crops is essential for effective prioritization of research and development. Yet previous estimates had limitations in distinguishing among contributing factors such as changing climate and new agronomic and genetic technologies. Here, we distinguished the separate contribution of these factors to yield advance using an extensive database collected from the largest irrigated maize-production domain in the world located in Nebraska (United States) during the 2005-to-2018 period. We found that 48% of the yield gain was associated with a decadal climate trend, 39% with agronomic improvements, and, by difference, only 13% with …

Biol 1411- Botany Laboratory Manual, Yolander R. Youngblood

Open Education Resources

Botany Laboratory Manual By – Yolander R.Youngblood, PhD.

This manual was reviewed by student editors, Ayanna Montegut and Ineceia Carter.

Table of Contents

1. The Plant Cell Lab

2. Mitosis Lab

3. Plant Growth and Development Lab

4. Simple Tissue Lab

5. Leaf Lab

6. Stem Lab

7. Root Lab

8. Evolution of Land Plants Lab

9. Bryophyte Lab

10. Fern Lab

11. Gymnosperm Lab

12. Angiosperm Lab

13. Appendix

a. Writing in the Scientific Notebook

b. How to use the Microscope

c. Plants and Their Structure

d. Additional Video, Lecture, and Lab Resources

Above- And Below-Ground Functional Trait Coordination In The Neotropical Understory Genus Costus, Eleinis Ávila-Lovera, Gregory R. Goldsmith, Kathleen M. Kay, Jennifer L. Funk

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The study of plant functional traits and variation among and within species can help illuminate functional coordination and trade-offs in key processes that allow plants to grow, reproduce and survive. We studied 20 leaf, above-ground stem, below-ground stem and fine-root traits of 17 Costus species from forests in Costa Rica and Panama to answer the following questions: (i) Do congeneric species show above-ground and below-ground trait coordination and trade-offs consistent with theory of resource acquisition and conservation? (ii) Is there correlated evolution among traits? (iii) Given the diversity of habitats over which Costus occurs, what is the relative contribution of …

Multi-Dimensional Leaf Phenotypes Reflect Root System Genotype In Grafted Grapevine Over The Growing Season, Zachery N. Harris, Mani Awale, Niyati Bhaakta, Daniel H. Chitwood, Anne Fennell, Emma Frawley, Laura L. Klein, Laszlo G. Kovacs, Misha Kwasniewski, Jason P. Londo, Qin Ma, The Ohio State University Miogicovsky, Joel F. Swift, Allison J. Miller

Agronomy, Horticulture and Plant Science Faculty Publications

Modern biological approaches generate volumes of multi-dimensional data, offering unprecedented opportunities to address biological questions previously beyond reach owing to small or subtle effects. A fundamental question in plant biology is the extent to which below-ground activity in the root system influences above-ground phenotypes expressed in the shoot system. Grafting, an ancient horticultural practice that fuses the root system of one individual (the rootstock) with the shoot system of a second, genetically distinct individual (the scion), is a powerful experimental system to understand below-ground effects on above-ground phenotypes. Previous studies on grafted grapevines have detected rootstock influence on scion phenotypes …

American Burying Beetle, Plant Richness, And Soil Property Responses To Collapse Of Juniperus Virginiana Woodlands With Fire, Alison K. Ludwig

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Grasslands are declining in the Great Plains due to land use changes, woody plant encroachment, and loss of historic fire cycles. Prescribed burn associations have utilized prescribed fire to collapse invading woodlands and allow the restoration of grasslands. This fire is considered “extreme” because it is capable of changing the structure and function of an ecosystem. Our study site is the Loess Canyons Experimental Landscape, a long-term, ecoregion-scale experiment to apply prescribed fire across the region to restore grasslands. The Nebraska Natural Legacy Project established the Loess Canyons ecoregion as a Biologically-Unique Landscape in 2005 with the state’s wildlife action …

Livestock Grazing Impacts On Crop And Soil Responses For Two Cropping Systems, Alyssa Kuhn

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Diversified crop, forage, and livestock systems are assumed to be more sustainable and economically competitive than traditional cropping systems. Objectives of this study were to determine effects of integrating grazing livestock into corn (Zea mays)-soybean (Glycine max (L.) Merr.) (C-S) and corn-soybean-wheat (Triticum aestivum L.) (C-S-W) cropping systems on plant population, grain yield, soil nutrients and soil carbon dioxide (CO₂) flux following winter grazing corn residue (both systems) and an oat (Avena sativa) cover crop (C-S-W only) planted after wheat. For the 2019 and 2020 production seasons, neither corn nor soybean plant …

A Method For Visualizing Water Flow Through Modified Root Zones, Dallas M. Williams

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

As the number of impervious surfaces in urban environments increases, the ability of modified root zones to infiltrate water is becoming more important. Current methods of tracing water flow through soil profiles include excavating large pits in situ or analyzing soil cores in the laboratory with computed tomography or magnetic resonance imaging. While useful, these methods may not be suitable for urban settings or practical in every laboratory. We propose a new method that is less invasive, does not require extensive technical equipment and can reliably trace water movement through the soil profile in order to calculate flow rate based …

Arkansas Corn And Grain Sorghum Performance Tests 2021, J. F. Carlin, R. B. Morgan, R. D. Bond

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers. The 2021 corn performance tests contained 70 hybrids and were conducted at the Northeast Research and Extension Center (NEREC) at Keiser, the Lon Mann Cotton Research Station (LMCRS) near Marianna, the Bell Farming Company (BFC) near Des Arc, the Rohwer Research Station (RRS) near Rohwer, and the Rice Research and Extension Center (RREC) near Stuttgart. The …

Comparing Organic And Conventional Yield Responses To Climate Variations, Joost Wilken Meyer

Graduate Theses and Dissertations

This thesis compares the responses of organic yields and conventional yields towards climate variations. To achieve this objective, weather variables such as growing season weather conditions (average temperature, precipitation, cloud cover, relative humidity, drought index), weather anomalies, the occurrence of severe or extreme droughts and excessive rainfalls, are combined with 23 data sets gathered from previous studies that compare organic and conventional yields from the same location and time periods. To narrow the scope, the thesis focuses on soybean, maize, and wheat production in Europe and North America. Study-level fixed-effects models are used to control for any time-invariant factors such …

Seeding Rate Effects On Forage Mass And Vegetation Dynamics Of Cool-Season Grass Sod Interseeded With Sorghum-Sudangrass, John A. Guretzky, D. D. Redfearn

Department of Agronomy and Horticulture: Faculty Publications

Interseeding annual warm-season grasses into perennial cool-season grasses has the potential to increase summer forage mass and nutritive value. Knowledge of how seeding rate affects annual warm-season grass establishment, forage mass, and vegetation dynamics remains limited. From 2016–2017, we conducted a field experiment evaluating the effects of seeding rates on sorghum-sudangrass (Sorghum bicolor x S. bicolor var. sudanense) density and forage mass and on the frequency of occurrence of plant species in cool-season grass sod in Lincoln, NE. The experiment had a completely randomized design consisting of six replicates of four seeding rates [0, 14, 28, and 35 …

Impact Of Urbanization Trends On Production Of Key Staple Crops, José F. Andrade, Kenneth G. Cassman, Juan I. Rattalino Edreira, Fahmuddin Agus, Abdullahi Bala, Nanyan Deng, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Urbanization has appropriated millions of hectares of cropland, and this trend will persist as cities continue to expand. We estimate the impact of this conversion as the amount of land needed elsewhere to give the same yield potential as determined by differences in climate and soil properties. Robust spatial upscaling techniques, well-validated crop simulation models, and soil, climate, and cropping system databases are employed with a focus on populous countries with high rates of land conversion. We find that converted cropland is 30–40% more productive than new cropland, which means that projection of food production potential must account for expected …

Influence Of Surfactant-Humectant Adjuvants On Physical Properties, Droplet Size, And Efficacy Of Glufosinate Formulations, Estefania G. Polli, Guilherme S. Alves, Jesaelen Gizotti De Moraes, Greg Robert Kruger

Department of Agronomy and Horticulture: Faculty Publications

Glufosinate efficacy is inconsistent among weed species and under environmental conditions that favor rapid droplet drying. Surfactant-humectant adjuvants could maximize glufosinate efficacy by increasing wetting and penetration into the leaf surface while decreasing evaporation rate (ER). However, there is a lack of information in the literature about the interaction of surfactant-humectants adjuvants with glufosinate. The objective of this study was to investigate the influence of surfactanthumectant adjuvants on the physical properties, droplet size, and efficacy of two glufosinate formulations. Laboratory, greenhouse, and field studies were conducted at the Pesticide Application Technology Laboratory of the University of Nebraska- Lincoln. Treatment design …

Prosopis Glandulosa Persistence Is Facilitated By Differential Protection Of Buds During Low- And High-Energy Fires, Heath D. Starns, Carissa L. Wonkka, Matthew B. Dickinson, Alexandra G. Lodge, Morgan L. Treadwell, Kathleen L. Kavanagh, Douglas R. Tolleson, Dirac L. Twidwell Jr, William E. Rogers

Department of Agronomy and Horticulture: Faculty Publications

Rangelands worldwide have experienced significant shifts from grass-dominated to woody-plant dominated states over the past century. In North America, these shifts are largely driven by overgrazing and landscape-scale fire suppression. Such shifts reduce productivity for livestock, can have broad-scale impacts to biodiversity, and are often difficult to reverse. Restoring grass dominance often involves restoring fire as an ecological process. However, many resprouting woody plants persist following disturbance, including fire, by resprouting from protected buds, rendering fire ineffective for reducing resprouting woody plant density. Recent research has shown that extreme fire (high-energy fires during periods of water stress) may reduce resprouting …

Insects And Their Laboulbeniales (Ascomycota, Fungi) Of Lake Eustis And Emeralda Marsh Conservation Area: A Case Study On Urbanization And Diversity, Patricia J. Kaishian

Purdue University Libraries Open Access Publishing Fund

A rapid biodiversity assessment of insects and associated Laboulbeniales fungi was conducted over the course of five nights in August, 2018, at two central Florida lakes: Lake Eustis and the nearby protected and restored National Natural Landmark, Emeralda Marsh Conservation Area (EMCA), which encompasses a portion of Lake Griffin. Lake Eustis was surveyed for Laboulbeniales in 1897 by mycologist Dr. Roland Thaxter but has not since been investigated. Because Lake Eustis has been urbanized, with the lake perimeter almost entirely altered by human development, the site offers a look into Laboulbeniales diversity across a 121-year timeline, before and after human …

Genome-Wide Dna Polymorphism Analysis And Molecular Marker Development For The Setaria Italica Variety “Ssr41” And Positional Cloning Of The Setaria White Leaf Sheath Gene Siwls1, Hui Zhang, Sha Tang, James C. Schnable, Qiang He, Yuanzhu Gao, Mingzhao Luo, Guanqing Jia, Baili Feng, Hui Zhi, Xianmin Diao

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide DNA polymorphism analysis and molecular marker development are important for forward genetics research and DNA marker-assisted breeding. As an ideal model system for Panicoideae grasses and an important minor crop in East Asia, foxtail millet (Setaria italica) has a high-quality reference genome as well as large mutant libraries based on the “Yugu1” variety. However, there is still a lack of genetic and mutation mapping tools available for forward genetics research on S. italica. Here, we screened another S. italica genotype, “SSR41”, which is morphologically similar to, and readily cross-pollinates with, “Yugu1”. High-throughput resequencing of “SSR41” …

A Chickpea Genetic Variation Map Based On The Sequencing Of 3,366 Genomes, Rajeev K. Varshney, Manish Roorkiwal, Shuai Sun, Prasad Bajaj, Annapurna Chitikineni, Mahendar Thudi, Narendra P. Singh, Xiao Du, Hari D. Upadhyaya, Diego Jarquin

Department of Agronomy and Horticulture: Faculty Publications

Zero hunger and good health could be realized by 2030 through effective conservation, characterization and utilization of germplasm resources1 . So far, few chickpea (Cicerarietinum) germplasm accessions have been characterized at the genome sequence level2 . Here we present a detailed map of variation in 3,171 cultivated and 195 wild accessions to provide publicly available resources for chickpea genomics research and breeding. We constructed a chickpea pan-genome to describe genomic diversity across cultivated chickpea and its wild progenitor accessions. A divergence tree using genes present in around 80% of individuals in one species allowed us to estimate the divergence of …

University Research On Winter Growing Of Container-Grown Strawberries Translates To Grower's Farm Trial, Stacy A. Adams, Ellen T. Paparozzi, Ryan Pekarek, Dave P. Lambe, George Meyer, M. Elizabeth Conley, Paul Read

Department of Agronomy and Horticulture: Faculty Publications

Strawberries are only seasonally available in Nebraska (NE). If an affordable heated structure can be designed, then opportunity exists to increase on-farm income by producing strawberries off-season during high-value market periods. A series of university greenhouse trials were conducted from 2010 to 2012. The varieties Evie-2 and Seascape were identified as being most productive under a low technology growing scheme. A new research project, which ran from fall to late spring (2013–2014), was designed to determine if this production scheme would translate to a commercial grower. Varieties Seascape and Evie-2 (each at two grades indicated by +) and San Andreas …

Control Of Acetolactate Synthase Inhibitor/Glyphosate-Resistant Palmer Amaranth (Amaranthus Palmeri) In Isoxaflutole/Glufosinate/Glyphosate-Resistant Soybean, Jasmine Mausbach, Suat Irmak, Debalin Sarangi, John L. Lindquist, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Palmer amaranth is the most problematic and troublesome weed in agronomic cropping systems in the United States. Acetolactate synthase (ALS) inhibitor and glyphosate-resistant (GR) Palmer amaranth has been confirmed in Nebraska and it is widespread in several counties. Soybean resistant to isoxaflutole/glufosinate/glyphosate has been developed that provides additional herbicide site of action for control of herbicide-resistant weeds. The objectives of this study were to evaluate herbicide programs for control of ALS inhibitor/GR Palmer amaranth and their effect on Palmer amaranth density and biomass, as well as soybean injury and yield in isoxaflutole/glufosinate/glyphosate-resistant soybean. Field experiments were conducted in a grower's …

Rebecca Fillyaw Ms Thesis Data For Publication In The Journal Sustainability, Rebecca Fillyaw, Melinda Donnelly, Linda Walters

CEELAB Research Data

By combatting erosion and increasing habitat, mangrove living shorelines are an effective alternative to hard-armoring in tropical and subtropical areas. An experimental red mangrove living shoreline was deployed within Mosquito Lagoon, Florida, using a factorial design to test the impact of mangrove age, breakwater presence, and mangrove placement on mangrove survival within the first year of deployment. Mixed mangrove age treatments were included to identify if seedling (11-month-old) survival could be enhanced by the presence of transitional (23-month-old) and adult (35 to 47-month-old) mangroves. Environmental factors were monitored to detect possible causes of mangrove mortalities. Approximately half (50.6%) of mangroves …

Algal Biodiversity Survey Of The Middle Chattahoochee River Using Dna Metabarcoding, Amanda Howell

Theses and Dissertations

Aquatic plants play vital roles in water systems by providing ecological services, and one group that is understudied is algae. Algae play a crucial role in water systems as bioindicators and primary producers. They can be challenging to identify with the naked eye, so researchers have been using DNA metabarcoding, where DNA belonging to specific taxa can be isolated and identified from water samples. The objective of this study was to conduct an algal biodiversity survey on the middle Chattahoochee River system in Georgia. Water samples (1L) were filtered and sent to RTL Genomics for sequencing and processing; the data …

Effects Of Redwood Bark And Leaf Leachate On Different Lichen Populations Found Within The Redwood Forest Of Arcata, Ca, Courtney Michelle Harris, Phoebe Hughes

IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt

No abstract provided.

2021 Summer/Fall Newsletter, Plaster Creek Stewards

Newsletters

Highlights:

Summer Supercrew

Plaster Creek Hike + Discovery

Green Team

Diversity Training

Visit with Chariman Ron Yob

Fall Event: Environmental Justice in Our Watershed

Planting Partners

Curb Cut Rain Garden Highlight

Identification And Validation Of High Ld Hotspot Genomic Regions Harboring Stem Rust Resistant Genes On 1b, 2a (Sr38), And 7b Chromosomes In Wheat, Shamseldeen Eltaher, Amira M.I. Mourad, P Stephen Baenziger, Stephen Wegulo, Vikas Belamkar, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Stem rust caused by Puccinia graminis f. sp. tritici Eriks. is an important disease of common wheat globally. The production and cultivation of genetically resistant cultivars are one of the most successful and environmentally friendly ways to protect wheat against fungal pathogens. Seedling screening and genome-wide association study (GWAS) were used to determine the genetic diversity of wheat genotypes obtained on stem rust resistance loci. At the seedling stage, the reaction of the common stem rust race QFCSC in Nebraska was measured in a set of 212 genotypes from F3:6 lines. The results indicated that 184 genotypes (86.8%) had different …

Spatial Frameworks For Robust Estimation Of Yield Gaps, Juan I. Rattalino Edreira, José F. Andrade, Kenneth G. Cassman, Martin K. Ittersum, Marloes P. Van Loon, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Food security interventions and policies need reliable estimates of crop production and the scope to enhance production on existing cropland. Here we assess the performance of two widely used ‘top-down’ gridded frameworks (Global Agro-ecological Zones and Agricultural Model Intercomparison and Improvement Project) versus an alternative ‘bottom-up’ approach (Global Yield Gap Atlas). The Global Yield Gap Atlas estimates extra production potential locally for a number of sites representing major breadbaskets and then upscales the results to larger spatial scales. We find that estimates from top-down frameworks are alarmingly unlikely, with estimated potential production being lower than current farm production at some …

Development Of A Genomic Prediction Pipeline For Maintaining Comparable Sample Sizes In Training And Testing Sets Across Prediction Schemes Accounting For The Genotype-By-Environment Interaction, Reyna Persa, Martin Grondona, Diego Jarquin

Department of Agronomy and Horticulture: Faculty Publications

The global growing population is experiencing challenges to satisfy the food chain supply in a world that faces rapid changes in environmental conditions complicating the development of stable cultivars. Emergent methodologies aided by molecular marker information such as marker assisted selection (MAS) and genomic selection (GS) have been widely adopted to assist the development of improved genotypes. In general, the implementation of GS is not straightforward, and it usually requires cross-validation studies to find the optimum set of factors (training set sizes, number of markers, quality control, etc.) to use in real breeding applications. In most cases, these different scenarios …