Open Access. Powered by Scholars. Published by Universities.®

Botany Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 5 of 5

Full-Text Articles in Botany

Tailed Forisomes Of Canavalia Gladiata: A New Model To Study Ca2+-Driven Protein Contractility, Winfried Peters, Michael Knoblauch, Stephen Warmann, Reinhard Schnetter, Amy Shen, William Pickard Jun 2007

Tailed Forisomes Of Canavalia Gladiata: A New Model To Study Ca2+-Driven Protein Contractility, Winfried Peters, Michael Knoblauch, Stephen Warmann, Reinhard Schnetter, Amy Shen, William Pickard

Winfried S. Peters

Background and Aims Forisomes are Ca2+-dependent contractile protein bodies that form reversible plugs in sieve tubes of faboid legumes. Previous work employed Vicia faba forisomes, a not entirely unproblematic experimental system. The aim of this study was to seek to establish a superior model to study these intriguing actuators.
Methods Existing isolation procedures were modified to study the exceptionally large, tailed forisomes of Canavalia gladiata by differential interference contrast microscopy in vitro. To analyse contraction/expansion kinetics quantitatively, a geometric model was devised which enabled the computation of time-courses of derived parameters such as forisome volume from simple parameters readily determined …


Reversible Birefringence Suggests A Role For Molecular Self-Assembly In Forisome Contractility, Winfried Peters, Reinhard Schnetter, Michael Knoblauch Apr 2007

Reversible Birefringence Suggests A Role For Molecular Self-Assembly In Forisome Contractility, Winfried Peters, Reinhard Schnetter, Michael Knoblauch

Winfried S. Peters

Forisomes are contractile protein bodies that control the effective diameter of the sieve elements of the faboid legumes by reversible, Ca2+-driven changes of shape. Forisomes consist of fibrils; we inferred from available electron-microscopical data (which necessarily provide images of fixed, non-functional forisomes) that a reversible assembly of ordered fibrillar arrays might be involved in the contractile mechanism. Here we examined functional forisomes isolated from Vicia faba L. by differential interference contrast microscopy and polarisation microscopy. We found them birefringent in the longitudinally expanded but not in the contracted state, showing ‘parallel extinction’ with the direction of vibration of the slow …


Effect Of Branch Position And Auxin Treatment On Clonal Propagation Of Tectona Grandis Linn. F., Azamal Husen, Mohinder Pal Jan 2007

Effect Of Branch Position And Auxin Treatment On Clonal Propagation Of Tectona Grandis Linn. F., Azamal Husen, Mohinder Pal

Dr. Azamal Husen

No abstract provided.


Metabolic Changes During Adventitious Root Primordium Development In Tectona Grandis Linn. F. Cuttings As Affected By Age Of Donor Plants And Auxin (Iba And Naa) Treatments, Azamal Husen Dr., Mohinder Pal Jan 2007

Metabolic Changes During Adventitious Root Primordium Development In Tectona Grandis Linn. F. Cuttings As Affected By Age Of Donor Plants And Auxin (Iba And Naa) Treatments, Azamal Husen Dr., Mohinder Pal

Dr. Azamal Husen

No abstract provided.


Incorporating Sheep Into Dryland Grain Production Systems I. Impact On Over-Wintering Larva Populations Of Wheat Stem Sawfly, Cephus Cinctus Norton (Hymenoptera: Cephidae), P. G. Hatfield, Sue L. Blodgett, T. M. Spezzano, H. B. Goosey, Andrew W. Lenssen, R. W. Kott, C. B. Marlow Jan 2007

Incorporating Sheep Into Dryland Grain Production Systems I. Impact On Over-Wintering Larva Populations Of Wheat Stem Sawfly, Cephus Cinctus Norton (Hymenoptera: Cephidae), P. G. Hatfield, Sue L. Blodgett, T. M. Spezzano, H. B. Goosey, Andrew W. Lenssen, R. W. Kott, C. B. Marlow

Andrew W. Lenssen

Wheat stem sawfly (WSS), Cephus cinctus Norton (Hymenoptera: Cephidae) is the most damaging insect pest to Montana’s $1 billion dollar per year grain industry. Current WSS control methods are either expensive, reduce wheat yields, or are not effective. Our objective was to compare burning, grazing, tilling, trampling and clipping wheat stubble fields on over-wintering WSS larval populations. Treatments were evaluated in three experiments using a randomized complete block design and four replications at each site. Eight, six, and two sites were used for Experiments 1, 2, and 3, respectively. Contrast statements were used to make pre-planned comparisons among treatments. For …