Botany Commons^™

Genetic Analysis Of Basal Stalk Rot Resistance Introgressed From Wild Helianthus Petiolaris Into Cultivated Sunflower (Helianthus Annuus L.) Using An Advanced Backcross Population, Zahirul I. Talukder, William Underwood, Christopher G. Misar, Xuehui Li, Gerald J. Seiler, Xiwen Cai, Lili Qi

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sclerotinia sclerotiorum is a serious pathogen causing severe basal stalk rot (BSR) disease on cultivated sunflower (Helianthus annuus L.) that leads to significant yield losses due to insufficient resistance. The wild annual sunflower species H. petiolaris, commonly known as prairie sunflower is known for its resistance against this pathogen. Sunflower resistance to BSR is quantitative and determined by many genes with small effects on the resistance phenotype. The objective of this study was to identify loci governing BSR resistance derived from H. petiolaris using a quantitative trait loci (QTL) mapping approach.

Methods: BSR …

Soybean Barcsoysnp6k: An Assay For Soybean Genetics And Breeding Research, Qijian Song, Long Yan, Charles Quigley, Edward Fickus, He Wei, Linfeng Chen, Faming Dong, Susan Araya, Jinlong Liu, David Hyten, Vincent R. Pantalone, Randall L. Nelson

Department of Agronomy and Horticulture: Faculty Publications

The limited number of recombinant events in recombinant inbred lines suggests that for a biparental population with a limited number of recombinant inbred lines, it is unnecessary to genotype the lines with many markers. For genomic prediction and selection, previous studies have demonstrated that only 1000–2000 genome-wide common markers across all lines/accessions are needed to reach maximum efficiency of genomic prediction in populations. Evaluation of too many markers will not only increase the cost but also generate redundant information. We developed a soybean (Glycine max) assay, BARCSoySNP6K, containing 6000 markers, which were carefully chosen from the SoySNP50K assay based …

Gwas: Fast-Forwarding Gene Identification And Characterization In Temperate Cereals: Lessons From Barley – A Review, Ahmad M. Alqudah, Ahmed Sallam, P. Stephen Baenziger, Andreas Börner

Department of Agronomy and Horticulture: Faculty Publications

Understanding the genetic complexity of traits is an important objective of small grain temperate cereals yield and adaptation improvements. Bi-parental quantitative trait loci (QTL) linkage mapping is a pow- erful method to identify genetic regions that co-segregate in the trait of interest within the research pop- ulation. However, recently, association or linkage disequilibrium (LD) mapping using a genome-wide association study (GWAS) became an approach for unraveling the molecular genetic basis underlying the natural phenotypic variation. Many causative allele(s)/loci have been identified using the power of this approach which had not been detected in QTL mapping populations. In barley (Hordeum …

Validation Of Qtl Mapping And Transcriptome Profiling For Identification Of Candidate Genes Associated With Nitrogen Stress Tolerance In Sorghum, Malleswari Gelli, Anji Reddy Konda, Kan Liu, Chi Zhang, Thomas E. Clemente, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Quantitative trait loci (QTLs) detected in one mapping population may not be detected in other mapping populations at all the time. Therefore, before being used for marker assisted breeding, QTLs need to be validated in different environments and/or genetic backgrounds to rule out statistical anomalies. In this regard, we mapped the QTLs controlling various agronomic traits in a recombinant inbred line (RIL) population in response to Nitrogen (N) stress and validated these with the reported QTLs in our earlier study to find the stable and consistent QTLs across populations. Also, with Illumina RNA-sequencing we checked the differential expression of …

Identification And Verification Of Qtl Associated With Frost Tolerance Using Linkage Mapping And Gwas In Winter Faba Bean, Ahmed Sallam, Mustapha Arbaoui, Mohamed El-Esawi, Nathan Abshire

Department of Agronomy and Horticulture: Faculty Publications

Frost stress is one of the abiotic stresses that causes a significant reduction in winter faba bean yield in Europe. The main objective of this work is to genetically improve frost tolerance in winter faba bean by identifying and validating QTL associated with frost tolerance to be used in marker-assisted selection (MAS). Two different genetic backgrounds were used: a biparental population (BPP) consisting of 101 inbred lines, and 189 genotypes from single seed descent (SSD) from the Gottingen Winter bean Population (GWBP). All experiments were conducted in a frost growth chamber under controlled conditions. Both populations were genotyped using the …

Mapping Qtls And Association Of Differentially Expressed Gene Transcripts For Multiple Agronomic Traits Under Different Nitrogen Levels In Sorghum, Malleswari Gelli, Sharon E. Mitchell, Kan Liu, Thomas E. Clemente, Donald P. Weeks, Chi Zhang, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Sorghum is an important C₄ crop which relies on applied Nitrogen fertilizers (N) for optimal yields, of which substantial amounts are lost into the atmosphere. Understanding the genetic variation of sorghum in response to limited nitrogen supply is important for elucidating the underlying genetic mechanisms of nitrogen utilization.

Results: A bi-parental mapping population consisting of 131 recombinant inbred lines (RILs) was used to map quantitative trait loci (QTLs) influencing different agronomic traits evaluated under normal N (100 kg.ha⁻¹ fertilizer) and low N (0 kg.ha⁻¹ fertilizer) conditions. A linkage map spanning 1614 cM was developed …