Agricultural Science Commons^™

Alfalfa In Rotation With Annual Crops Reduced Nitrate Leaching Potential, Arshdeep Singh, Tahseen Afzal, Bryan Woodbury, Charles Wortmann, Javed Iqbal

Department of Agronomy and Horticulture: Faculty Publications

Rotation of perennial alfalfa (Medicago sativa L.) with annual crops has the potential to reduce nitrate-nitrogen (NO₃-N) in the vadose zone and increase soil organic carbon (SOC) sequestration. The objective of this study was to determine the long-term effects on SOC, NO₃-N, ammonium-N (NH₄-N), and soil water in the 7.2 m depth with an alfalfa rotation compared with continuous corn (Zea mays L.). Soils from six pairs of alfalfa rotation versus continuous corn observation points were sampled to 7.2 m depth in 0.3 m increments. The uppermost 0.3 m was divided into …

Soybean Seed Protein Concentration Is Limited By Nitrogen Supply In Tropical And Subtropical Environments In Brazil, E. H. Figueiredo Moura Da Silva, N. Cafaro La Menza, G. G. Munareto, A. J. Zanon, Santos Carvalho, F. R. Marin

Department of Agronomy and Horticulture: Faculty Publications

Soybean production contributes to ca. 60% of global plant-based protein used for food and feed. Brazil is the largest soybean producer and exporter, with 60% from tropical and 40% from subtropical environments. Nitrogen (N) can play an essential role in the storage of proteins in seeds; thus, it could be a key factor in increasing the quantity and quality of seeds in high-yielding soybean crops. Unlike in temperate environments, there is a gap of knowledge on whether soybean grown under tropical and subtropical climates are limited by N-fertilization to sustain the seed yield increase without detriments in seed protein concentration. …

Potential Yield Of Cocksfoot (Dactylis Glomerata) Monocultures In Response To Irrigation And Nitrogen, A. Mills, D. J. Moot, R. L. Lucas, P. D. Jamieson, B. A. Mckenzie

IGC Proceedings (1977-2023)

Cocksfoot is a widely sown grass in temperate pastures. However, while potential yield of cocksfoot can exceed 28 t DM/ha per year, it is often restricted by water, temperature and nitrogen (N). Of these, Peri et al. (2002) showed that N was severely limiting in all seasons. The aim of this study was to confirm the potential yield of cocksfoot and quantify the extent of yield reductions due to environmental constraints.

Diverse Forage Mixtures Effect On Herbage Yield, Sward Composition, And Dairy Cattle Performance, M. A. Sanderson, K. Soder, N. Brzezinski, S. Goslee, H. Skinner, M. Wachendorf, F. Taube, L. Muller

IGC Proceedings (1977-2023)

Managing complex mixtures of plants to take advantage of spatial and temporal variability in land and climate may be one ecological approach to increase productivity of pastures. We tested the hypothesis that complex mixtures of forage species would yield more herbage and reduce weed competition compared with a simple grass-legume mixture in grazed pastures.

Hygienic Value And Mycotoxins Level Of Grass Silage In Bales For Horses, A. Potkański, J. Grajewski, K. Raczkowska-Werwińska, B. Miklaszewska, A. Gubała, M. Selwet, M. Szumacher-Strabel

IGC Proceedings (1977-2023)

Mycotoxins are secondary metabolites of moulds which have adverse effects on humans, animals, and crops and result in illnesses and economic losses. The toxins may occur in storage under conditions favourable for the growth of the toxin-producing fungus or fungi. The highest forage concentration of toxins was found in horizontal storage methods such as bunker silos and feed piles, which were left open to oxygen. In any fermentation storage system, temperature and the presence of moisture is sufficient for toxin production. In a plastic covered storage system, oxygen penetration is slowed but not eliminated. The longer silage is stored, the …

Participatory Approach To Common Use Grazing Management In Dry Area Developing Countries, J. Tiedeman, A. Larbi, F. Ghassali, N. Battikha

IGC Proceedings (1977-2023)

Key points

1. Range restoration technology is available but useless when not followed by management.

2. Institutional mechanisms for grazing management are needed for communal range.

3. Community participatory approaches help pastoralists better manage rangeland grazing.

The Effects Of Maize And Whole Crop Wheat Silages And Quality Of Grass Silage On The Performance Of Lactating Dairy Cows, D. C. Patterson, D. J. Kilpatrick

IGC Proceedings (1977-2023)

Patterson et al. (2004) obtained positive milk production responses to the inclusion of maize silage in grass silage-based diets under Northern Ireland conditions. By contrast, while inclusion of fermented whole crop wheat increased total forage intake, it had no significant effect on milk production of dairy cows. More recently, a newer technique has been developed of harvesting the wheat crop at high DM content, with milling of the grain during harvesting and treatment with an urea/urease mixture at ensiling (alkalage treatment). The aim of the present study was to investigate the milk production potential of high DM whole crop …

Polyphenol Oxidase Activity And In Vitro Proteolytic Inhibition In Grasses, J. M. Marita, R. D. Hatfield, G. E. Brink

IGC Proceedings (1977-2023)

Harvesting and storing high quality forage in the cool humid regions remains a challenge due to the potential for protein degradation during ensiling. Red clover is an exception as high protein levels are maintained during ensiling. Decreased proteolytic activity in red clover is due to polyphenol oxidase (PPO) activity and appropriate o-diphenol substrates (Jones et al., 1995, Sullivan et al., 2004). This project was undertaken to determine if PPO activity is present in a range of grasses and the potential role in proteolytic inhibition in the presence of the o-diphenol caffeic acid.

The Effect Of Dry Matter Content And Inoculation With Lactic Acid Bacteria On The Residual Water Soluble Carbohydrate Content Of Silages Prepared From A High Sugar Grass Cultivar, D. R. Davies, D. K. Leemans, E. L. Bakewell, R. J. Merry

IGC Proceedings (1977-2023)

The introduction of new perennial ryegrass cultivars bred for high water soluble carbohydrate (WSC) content has created opportunities for improving the quality of grass silage, by not only providing adequate WSC for a good fermentation, but also sufficient to leave a higher residual level of WSC in the mature silage. High WSC silages have the potential to provide readily available energy during the early stages of rumen fermentation to balance energy and nitrogen supply and optimise rumen microbial growth. (Merry et al. 2002). The aim was to examine the effect of wilting and silage inoculants on the residual WSC content …

Using The Red Clover Polyphenol Oxidase Gene To Inhibit Proteolytic Activity In Lucerne, R. D. Hatfield, M. L. Sullivan, R. E. Muck

IGC Proceedings (1977-2023)

Preserving high quality forage in cool humid regions of agricultural production remains a challenge due to potentially high levels of protein degradation during ensiling. Red clover is an exception maintaining its high protein levels during ensiling. Decreased proteolytic activity in red clover is due to polyphenol oxidase (PPO) activity and appropriate o-diphenol substrates (Jones et al., 1995, Sullivan et al., 2004). This work highlights potential strategies for utilising PPO as a means of decreasing proteolytic degradation during the ensiling of lucerne and other forages.

New Results On Inhibition Of Clostridia Development In Silages, E. Kaiser, K. Weiß, I. Polip

IGC Proceedings (1977-2023)

The prevention of clostridial activity in silages is one of the most important aims in silage making. Clostridial activity in silages is especially expressed as the occurrence of butyric acid and as increased content of clostridial spores. A rapid reduction in the pH value at the beginning of fermentation process is considered as the most important factor for inhibition of clostridial development. It is assumed, that, if the “critical pH value” will be quickly achieved, clostridial activity in silages can be stopped. In experiments concerning the fermentation process it was found that the effect of acidification and dry matter content …

Ensilability And Silage Quality Of Different Cocksfoot Varieties, U. Wyss

IGC Proceedings (1977-2023)

Various factors determine the ensilability of plant crops. In addition to dry matter content, sugar and protein content, buffering capacity, plant structure, soil contaminations and epiphytic microflora are also important. In Switzerland, various mixtures and also pure swards are continuously revised on the basis of results from variety testing programs. The main parameters for these tests are the yield and nutrient contents. However, in terms of ensilability and silage quality, no systematic tests have been carried out. For this reason, we have tested the ensilability of different cocksfoot varieties as well as the quality of the silages.

Effects Of Silage Preparation And Microbial Silage Additives On Biogas Production From Whole Crop Maize Silage, M. Neureiter, C. Perez Lopez, H. Pichler, R. Kirchmayr, R. Braun

IGC Proceedings (1977-2023)

Biogas applications based on the production of energy from renewable resources have emerged in the past years due to several countries setting quotas for bioenergy thus promoting anaerobic digestion for heat and electricity generation. Maize is one of the most common substrates for biogas production based on energy crops because of the high yields per hectare with ensiling as the preferred method for storage. Experiments were performed to investigate whether conditions during the silage fermentation and the addition of starter cultures can affect the biogas yields.

Ensiling Characteristics Of Sudangrass Silage Treated With Green Tea Leaf Waste Or Green Tea Polyphenols, M. Kondo, K. Kita, H. Yokota

IGC Proceedings (1977-2023)

Green tea waste (GTW), emitted from beverage companies manufacturing tea drinks, contains high crude protein (CP) and polyphenols. Kondo et al. (2004) showed that GTW addition to forage ensiling enhanced lactic acid fermentation and decreased pH value. Ishihara et al. (2001) showed that high counts of Lactobacillus species were maintained and the counts of clostridia were decreased in the intestinal microflora of animals fed the diet containing green tea polyphenols (GTP). It is hypothesised that GTP might activate lactic acid bacteria and enhance silage fermentation. This study was conducted to evaluate the potential of GTW and GTP as silage …

A 16s Rdna-Based Quantitative Assay For Monitoring Lactobacillus Plantarum In Silage, M. Klocke, K. Mundt, C. Idler, P. O'Kiely, S. Barth

IGC Proceedings (1977-2023)

Ensilage of herbaceous biomass can be enhanced by applying pre-selected fermentative bacteria, however insufficient is known about the population dynamics of such starter cultures under a range of ensiling conditions. Classical methods for species-specific quantification of bacteria are labour intensive. An alternative approach is the detection of bacteria based on molecular markers for species-specific regions within their genomic DNA (e.g. the 16S rDNA sequence). In this study, a quantitative marker assay using the real-time PCR technique (Q-PCR) is described for Lactobacillus plantarum, a bacterium often used for silage starter cultures.

A Comparison Of The Efficacy Of An Ultra-Low Volume Applicator For Liquid-Applied Silage Inoculants With That Of A Conventional Applicator, G. Marley, G. Pahlow, H. -H. Herrmann, T. R. Owen

IGC Proceedings (1977-2023)

Liquid-applied silage inoculants are normally sprayed onto forages cut for ensiling at application rates from 1 to 3 l/t. Applicator tanks can require frequent re-filling, especially with large self-propelled forage harvesters having harvest rates in excess of 1000 t/d. This can be an issue for fields remote from the farm, for areas with restricted water availability and for contractors paid by the area harvested. This study was conducted to assess the efficacy of inoculant distribution on the crop using a simple, ultra-low volume (ULV) applicator compared with a conventional liquid-applied silage inoculant applicator.

Improving The Aerobic Stability Of Whole-Crop Cereal Silages, I. Filya, E. Sucu, A. Karabulut

IGC Proceedings (1977-2023)

Whole-crop cereal silages, such as wheat, sorghum, and maize are susceptible to aerobic deterioration, especially in warm climates. This is because aerobic yeasts are the most active at 20-30°C (Ashbell et al., 2002). Therefore, it is very important to find suitable additives that inhibit fungi and protect the silage upon aerobic exposure. Propionibacterium acidipropionici is propionic acid bacteria (PAB), which produce propionic and acetic acid in silage. Results with these micro-organisms in laboratory studies were promising with regard to aerobic stability. The purpose of the present work was to study the effects of PAB, lactic acid bacteria (LAB) and …

Microbial Changes And Aerobic Stability In High Moisture Maize Silages Inoculated With Lactobacillus Buchneri, R. A. Reis, E. O. Almeida, G. R. Siqueira, T. F. Bernardes, E R. Janusckiewicz, M. T. P. Roth

IGC Proceedings (1977-2023)

Oxygen exposure changes microbial and chemical profiles of silages after silo opening. Yeast and fungi are the main active microorganisms responsible by consumption of nutrients and fermentative residual products, which increases temperature in the ensilaged mass. The Lactobacillus buchneri (L. buchneri) has been considered an aerobic stability controller. This work aimed to evaluate the effect of L. buchneri in ensilage process of high moisture maize.

Aerobic Stability And Nutritive Value Of Low Dry Matter Maize Silage Treated With A Formic Acid-Based Preservative, I. Filya, E. Sucu, A. Karabulut

IGC Proceedings (1977-2023)

Aerobic stability is one of the major problems of the ensiling process, especially in warm climates. Ashbell et al. (2002) have shown that at 30°C, the development of aerobic yeast and moulds in silages is most intensive. In Turkey all silages are susceptible to air penetration during storage and unloading with a large proportion of the silage spoiled and in extreme cases all the silage is spoiled. The purpose of the present work was to study the effects of formic acid-based preservative (FAB; Kemisile® 2000, Kemira Oyj-Industrial Chemicals, Finland) on the aerobic stability and nutritive value of maize silage.

An In Vitro Study On The Influence Of Residual Sugars On Aerobic Changes In Grass Silages, S. D. Martens, G. Pahlow, J. M. Greef

IGC Proceedings (1977-2023)

How do residual sugars in high dry matter grass silages influence microbial metabolism? To answer this question a simple laboratory method was developed using pH as main indicator for aerobic changes.

The Effects Of The Growth Stage And Inoculant On Fermentation And Aerobic Stability Of Whole-Plant Grain Sorghum Silage, E. Tabacco, G. Borreani

IGC Proceedings (1977-2023)

Grain sorghum (Sorghum bicolor) is well adapted to environments with limited rainfall and low soil fertility. Today, on dry land, improved grain sorghum hybrids may be a valid alternative to maize silage and they may give DM yields and digestible energy that are comparable to maize, but at lower production costs (Legarto, 2000). Harvesting crops for silage at an early stage of maturity (low DM content) may result in silage with a higher acid content and low nutritional quality, while harvesting crops at a later stage of maturity may make the forage more difficult to chop and pack. …

Perennial Ryegrasses Bred For Contrasting Sugar Contents: Manipulating Fermentation And Aerobic Stability Using Wilting And Additives (1) (Eu Fp V -Project ‘Sweetgrass’), P. O'Kiely, H. Howard, G. Pahlow, R. J. Merry, F. P. O'Mara

IGC Proceedings (1977-2023)

Higher concentrations of water-soluble carbohydrate (WSC) in silage offer ruminant nutrition and environmental attractions. Both successful field wilting and alternative silage additives provide the opportunity to manipulate silage WSC by modifying fermentation and/or improving aerobic stability. This experiment evaluated the fermentation and aerobic stability of silages made from perennial ryegrass cultivars of high or normal WSC genotype that differed in field wilting or additive use.

Perennial Ryegrasses Bred For Contrasting Sugar Contents: Manipulating Fermentation And Aerobic Stability Of Unwilted Silage Using Additives (2) (Eu-Project ‘Sweetgrass’), H. Howard, P. O'Kiely, G. Pahlow, F. P. O'Mara

IGC Proceedings (1977-2023)

Grass cultivars bred for elevated concentrations of water-soluble carbohydrate (WSC) could have improved silage preservation but possibly disimproved aerobic stability. Additives can be used to manipulate fermentation and thereby increase silage WSC. They can also influence aerobic stability. This experiment evaluated the fermentation and aerobic stability of unwilted silages made from perennial ryegrass cultivars of high or normal WSC genotype that differed in additive use.

Perennial Ryegrasses Bred For Contrasting Sugar Contents: Manipulating Fermentation And Aerobic Stability Of Wilted Silage Using Additives (3) (Eu-Project ‘Sweetgrass’), H. Howard, P. O'Kiely, G. Pahlow, F. P. O'Mara

IGC Proceedings (1977-2023)

Rapid field-drying of grass prior to successful ensilage restricts fermentation and can assist preservation, but can consequently result in silages that are prone to aerobic deterioration at feedout. Additives that directly (e.g. potassium sorbate or sodium benzoate) or indirectly (e.g. formic acid or Lactobacillus plantarum, via manipulation of fermentation) alter yeast activity at feedout could modify silage aerobic stability. This experiment evaluated the fermentation and aerobic stability of wilted silages made from perennial ryegrass cultivars of high or normal water soluble carbohydrate (WSC) genotype that differed in additive use.

The Effect Of Additive Containing Formic Acid On Quality And Aerobic Stability Of Silages Made Of Endophyte-Infected Green Forage, L. Podkówka, J. Mikołajczak, E. Staszak, P. Dorszewski

IGC Proceedings (1977-2023)

Festuca species grasses are very often infected with endophytic fungi Neothypodium, that produce ergotic alkaloids, peramins etc. (Podkówka et al., 2003). Produced green forage can be dangerous to animals. The basic preservation method of such green forages is ensiling, especially with organic acids addition. Organic acids demonstrate destructive action on fungal organisms, as well as influence the quality and aerobic stability of forage. The objective of this study was to determine if the preservation of endophyte-infected green forage by ensiling with formic acid affects quality and aerobic stability of produced fodder.

Combining Corn N Recommendation Tools For An Improved Economical Optimal Nitrogen Rate Estimation, Curtis J. Ransom, Newell R. Kitchen, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabián G. Fernández, David W. Franzen, Carrie A. M. Laboski, David Brenton Myers, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Improving corn (Zea mays L.) nitrogen (N) rate fertilizer recommendation tools can improve farmers’ profits and mitigate N pollution. Numerous approaches have been tested to improve these tools, but to date improvements for predicting economically optimum N rate (EONR) have been modest. This work’s objective was to use ensemble learning to improve our estimation of EONR (for a single at-planting and split N application timing) by combining multiple corn N recommendation tools. The evaluation was conducted using 49 corn N response trials from eight states in the US Corn Belt and three growing seasons (2014–2016). Elastic net and decision …

De Novo Assembly And Comparative Analyses Of Mitochondrial Genomes In Piperales, Runxian Yu, Xudong Chen, Lingjie Long, Matthias Jost, Ran Zhao, Lumei Liu, Jeffrey P. Mower, Claude W. Depamphilis, Stefan Wanke, Yuannian Jiao

Department of Agronomy and Horticulture: Faculty Publications

The mitochondrial genome of Liriodendron tulipifera exhibits many ancestral angiosperm features and a remarkably slow evolutionary rate, while mitochondrial genomes of other magnoliids remain yet to be characterized. We assembled nine new mitochondrial genomes, representing all genera of perianth-bearing Piperales, as well as for a member of the sister clade: three complete or nearly complete mitochondrial genomes from Aristolochiaceae and six additional draft assemblies including Thottea, Asaraceae, Lactoridaceae, and Hydnoraceae. For comparative purpose, a complete mitochondrial genome was assembled for Saururus, a member of the perianth-less Piperales. The average number of short repeats (50–99 bp) was much larger …

Effect Of Residual Sugar In High Sugar Grass Silages On Aerobic Stability, G. Pahlow, R. J. Merry, P. O'Kiely, T. M. Pauly, J. M. Greef

IGC Proceedings (1977-2023)

New varieties of Lolium perenne, bred for high sugar content, can contain up to 30% of water soluble carbohydrates (WSC). Only a fraction of such high contents are metabolised during a normal fermentation and the high residual sugar content (RSC) of these silages can improve the efficiency of use of nitrogen by ruminants. However, these RSC at opening for feed-out could be preferentially metabolised relative to fermentation products by all aerobically growing fungi and bacteria present on the forage. A high RSC thus can increase the risk of aerobic deterioration over that of extensively fermented silages, containing predominantly organic …

Recent Developments In Methods To Characterise The Chemical And Biological Parameters Of Grass Silage, R. S. Park, R. E. Agnew, M. G. Porter

IGC Proceedings (1977-2023)

Key points

1. Chemical analysis of forages is expensive, time consuming, environmentally unfriendly and relates poorly to the feed value for production purposes.
2. In vivo characterisation of animal feed is not a feasible option in terms of cost and analysis time.
3. NIRS is a rapid, non destructive, environmentally friendly, multi-analytical technique which can estimate the nutritive value of the feed.
4. NIRS predictive equations developed on a master instrument can be transferred to local and international sites.
5. Future assessment of forages necessitates rapid, stable, instrumentation for ‘in field’ studies.

The Effect Of Chop Length And Additive On Silage Intake And Milk Production In Cows, V. Toivonen, T. Heikkilä

IGC Proceedings (1977-2023)

Effects of reduced silage chop length on silage intake and milk production by dairy cows have been variable. Chopping of grass at harvest generally improves silage fermentation quality and consequently intake. The objective of this study was to assess the effect of chopping flail harvested silage ensiled with acid or biological additive prior to feeding on intake, milk yield and milk composition in dairy cows.