Agricultural Science Commons^™

Purification And Characterization Of Intrauterine Folate-Binding Proteins From Swine, J.L. Vallet, R.K. Christenson, H.G. Klemcke

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Folate-binding proteins (FBP) from Day 60 pseudopregnant uterine flushings and Day 60 allantoic fluid were purified by affinity chromatography on folate-Sepharose followed by G–100 Sephadex chromatography. FBP from uterine flushings had a molecular weight of 20 000; the N-terminal sequence was FNWDHXGKMEPAXKRHFXXXTXLYX, which is 72% identical to bovine milk FBP beginning at amino acid 64. Allantoic fluid FBP had a molecular weight of 30 000; and the N-terminal sequence ARAKTDMLNVXMDAKHHKPKPSXED, which is 68% identical to bovine milk FBP starting at amino acid 4. Scatchard analysis of purified allantoic fluid FBP using [³H]folic acid as ligand indicated a dissociation …

Morphological Study Of Cultivated Cowpea Vigna Unguiculata (L.) Walp. Importance Of Ovule Number And Definition Of Cv Gr Melanophthalmus, Rémy Stéphane Pasquet

All Peer-Reviewed Publications

More than 400 cultivated cowpea accessions were studied using 29 morphophysiological characters. The analysis of data contrasts evolved cultivars and more primitive cultivars (and wild forms) according to seed size on the one hand and early and late flowering (under inductive conditions) on the other hand. The latter character is markedly correlated with ovule number. Photosensitive and early-flowering photoindependent cultivars have 11-17 ovules per pod and late flowering photoindependent cultivars have 16-25 ovules per pod. The analysis leads to assigning the accessions studied to the following different cultigroups: cv gr Unguiculata, cv gr Sesquipedalis, cv gr Biflora and cv gr …

Focus On The Future Agriculture Western Australia's Strategic Directions 1998 - 2008, Agriculture Western Australia

Reports

Strong growth in global demand combined with productivity gains, product innovation and sustainability improvements throughout the 1990s enable Western Australia agriculture to look to the 21st century with confidence.

This optimistic outlook is largely the result of innovation across the agricultural industries based on research, development and extension - activities in which Agriculture Western Australia continues to be the State's leading provider.

Focus on the Future is ambitious, and sends a unequivocal message about our strategic directions and the rationale guiding the agency in allocating resources to those areas which offer the biggest benefits to the Western Australian agriculture, food …

Atrazine, Deethylatrazine, And Deisopropylatrazine Persistence Measured In Groundwater In Situ Under Low-Oxygen Conditions, Sharon Papiernik, Roy Spalding

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The degradation of atrazine [2-chloro-4-(ethylamino)-6-isopropylamino-1,3,5-triazine], deethylatrazine [DEA; 2-amino-4-chloro-6-(isopropylamino)-1,3,5-triazine], and deisopropylatrazine [DIA; 2-amino-4-chloro-6-(ethylamino)-1,3,5-triazine] was assessed under limited oxygen conditions using in situ microcosms. Denitrification was induced in a shallow sand and gravel aquifer to measure the potential for degradation of atrazine, DEA, and DIA under low-O2 conditions. The dissolved oxygen content decreased from 7-8 mg/L to ≤1 mg/L within 4 days and remained ≤ mg/L for the remainder of the 45-day experiment. Atrazine, DEA, and DIA concentrations (normalized to the bromide concentration at each sampling time to account for dilution) did not show a significant decrease with time, indicating that these …

Technique For Rlem Screening In Glasshouse, Krzysztof S. Gajda

Grain and other field crops published reports

The redlegged earth mite (RLEM) Halotydeus destructor (Tucker) (Acarina: Penthaleidae) is an introduced, major pest of crops and pastures across south Australia. RLEM adversely effect pasture by destroying legume seedlings in germinating pastures and reducing pasture production and seed yield. One of the ways to reduce the effect of RLEM is by breeding legume cultivars with RLEM resistance. This involves screening of many hundreds of potentially resistant plants and lines in a short time. In WA, at Agriculture Western Australia, South Perth, a screening program for resistance to RLEM in subterranean clover (Trifolium subterranean) and annual medics (Medicago ssp.) has …

Evidence Of Antibiosis Resistance To The Spotted Stemborer, Chilo Partellus (Swinhoe) In A Maize (Zea Mays L.) Population, C. J.M. Mutinda, K. N. Saxena, S. O. Ajala, P. O. Aviecho

All Peer-Reviewed Publications

S2 line and S2 test-cross recurrent selection procedures were initiated to improve the resistance of a maize (Zen mays L.) population to the spotted stemborer, Chllo partclltis (Swinhoe) (Lepidoptera: Pyralidae). Thirty-five selections developed from the population were evaluated for resistance to the stemborer in western Kenya. Resistance was rated according to leaf feeding (LF), stem tunnelling (ST) and deadheart formation (DH) following artificial infestation with first-instar C. pnrtcUus. Larval establishment and growth were also assessed. The reduction in foliar damage ratings, percentage stem length tunnelled, and percentage of plants showing deadheart after one cycle of recurrent selection were 4.38 %, …

Selective And Organotypic Culture Of Intrahepatic Bile Duct Cells From Adult Pig Liver, Neil C. Talbot, Thomas J. Caperna

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Secondary culture of nontransformed bile duct epithelium has been difficult to achieve. STO feeder cell-dependent secondary cultures of adult pig bile duct cells were established from primary cultures of adult pig liver cells. Adult pig hepatocytes exhibited limited or no replication and were lost from the secondary culture at Passage 3 or 4. In contrast, adult pig bile duct cells replicated and were carried for 4-8 passages in secondary culture. A simple method to produce nearly pure pig intrahepatic bile duct cultures was first to freeze a relatively crude liver cell preparation. Upon subsequent thawing, all hepatocytes and most macrophages …

Plant Hemoglobins, Raul Arrendondo-Peter, Mark S. Hargrove, Jose F. Moran, Gautam Sarath, Robert V. Klucas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Hbs are ubiquitous proteins in most organisms, including bacteria, protozoans, fungi, plants, and animals (Vinogradov et al., 1993; Bolognesi et al., 1997). The known and predicted roles of Hbs in each organism encompass functions that include the reversible binding of gaseous ligands and the ability to bind other cellular molecules.

Northern Australia Quarantine Strategy. Weeds Target List, B. M. Waterhouse, A A. Mitchell

Biosecurity published reports

Second edition.

The Northern Australia Quarantine Strategy (NAQS) was established in 1989 as an outcome of a major evaluation of quarantine in Australia (AGPS 1987, 1988). NAQS activities include surveying for weeds,insect pests and diseases of plants; and pests and diseases of animals in northern Australia, Papua New Guinea and Indonesia. The primary goal is to provide early detection and warning of new quarantine threats or incursions.

A targetted approach was adopted for all pests, diseases and weeds. For each category of pest, experts in the discipline compiled lists of organisms not known from Australia but known to occur in …

Growth Traits Among Sorghum Genotypes In Response To Aluminum, J. H. Bernal, R. B. Clark

Department of Agronomy and Horticulture: Faculty Publications

Aluminum (Al) has many detrimental effects on plant growth, and shoots and roots are normally affected differently. A study was conducted to determine differences among sorghum [Sorghum bicolor (L.) Moench] genotypes with broad genetic backgrounds for growth traits of plants grown at 0,200,400,600, and 800 uM Al in nutrient solutions (pH 4.0). Genotypes were categorized into "Al-sensitive", "intermediate Al-tolerant", "Al-tolerant", and SC 283 (an Al-tolerant standard). As Al increased, shoot and root dry matter (DM), net main axis root length (NMARL), and total root length (TRL) became lower than controls (0 Al). Aluminum toxicity and/or nutrient deficiency symptoms become …

Inactivation Of Soybean Peroxidase During Sodium Azide Oxidation: A Comparative Study, Mohamed Yehia Zakaria Aboul Eish

Dissertations and Theses @ UNI

Azide ion is a mechanism-based inactivator of a number of peroxidases. However, there are often variations in the manner in which inactivation occurs. Soybean Peroxidase (SBP) is found in the plant's seed coat. Unlike many other peroxidases, a single enzyme rather than several isozymes are produced thus making more simple the purification and characterization of the enzyme. In this research it was shown that, as with other peroxidases, SBP is inactivated by azide during turnover. During azide oxidation SBP forms a ferrous-NO intermediate. In this respect, SBP is similar to the lignin peroxidases from Phanerochaete chrysosporium which also form ferrous-NO …

Yuna Farm Improvement Group - Conservation Farm Planning Manual, Mike Clarke, Cecilia Mcconnell, Nancye Gannaway

Natural resources published reports

A landcare group formed on Tuesday, 30 June 1998 due to growing concern about land degradation. The main interests and objectives of the group where discuss and were outlined as follows:

1. Wind Erosion

2. Soil Acidity

3. Soil Degradation

4. Remnant Vegetation Decline

5. Tree Placement and Survival

6. Oil Mallees

7. Water Erosion

8. Non Wetting Soils

9. Salinity

10. Waterlogging

The first step toward addressing these issues was to organise two workshops with Mike Clarke, Cecilia McConnell and Nancye Gannaway from Agriculture Western Australia. The first workshop was held on the 4/8/98 and the second on the …