Agricultural Science Commons^™

Cell Wall Proteome In The Maize Primary Root Elongation Zone. Ii. Region-Specific Changes In Water Soluble And Lightly Ionically Bound Proteins Under Water Deficit1[W][Oa], Jinming Zhu, Sophie Alvarez, Ellen L. Marsh, Mary E. Lenoble, In-Jeong Cho, Mayandi Sivaguru, Sixue Chen, Henry T. Nguyen, Yajun Wu, Daniel P. Schachtman, Robert E. Sharp

Department of Agronomy and Horticulture: Faculty Publications

Previous work on the adaptation of maize (Zea mays) primary roots to water deficit showed that cell elongation is maintained preferentially toward the apex, and that this response involves modification of cell wall extension properties. To gain a comprehensive understanding of how cell wall protein (CWP) composition changes in association with the differential growth responses to water deficit in different regions of the elongation zone, a proteomics approach was used to examine water soluble and loosely ionically bound CWPs. The results revealed major and predominantly region-specific changes in protein profiles between well-watered and water-stressed roots. In total, 152 …

Map Location Of The Rpp1 Locus That Confers Resistance To Soybean Rust In Soybean, D. L. Hyten, G. L. Hartman, R. L. Nelson, R. D. Frederick, V. C. Concibido, J. M. Narvel, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean rust (SBR), caused by Phakopsora pachyrhizi, was first discovered in North America in 2004 and has the potential to become a major soybean [Glycine max (L.) Merr.] disease in the USA. Currently, four SBR resistance genes have been identifi ed but not mapped on the soybean genetic linkage map. One of these resistance genes is the Rpp1 gene, which is present in the soybean accession PI 200492. The availability of molecular markers associated with Rpp1 will permit marker-assisted selection and expedite the incorporation of this gene into U.S. cultivars. We compared simple sequence repeat (SSR) markers between …

Barcsoysnp23: A Panel Of 23 Selected Snps For Soybean Cultivar Identification, M. S. Yoon, Q.J. Song, I. Y. Choi, James E. Specht, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

This report describes a set of 23 informative SNPs (BARCSoySNP23) distributed on 19 of the 20 soybean linkage groups that can be used for soybean cultivar identification. Selection of the SNPs to include in this set was made based upon the information provided by each SNP for distinguishing a diverse set of soybean genotypes as well as the linkage map position of each SNP. The genotypes included the ancestors of North American cultivars, modern North American cultivars and a group of Korean cultivars. The procedure used to identify this subset of highly informative SNP markers resulted in a significant increase …

Ethylene Involvement In The Regulation Of The H+-Atpase Csha1 Gene And Of The New Isolated Ferric Reductase Csfro1 And Iron Transporter Csirt1 Genes In Cucumber Plants, Brian M. Waters, Carlos Lucena, Francisco J. Romera, Gena G. Jester, April N. Wynn, Carmen L. Rojas, Esteban Alcántara, Rafael Pérez-Vicente

Department of Agronomy and Horticulture: Faculty Publications

In previous works using ethylene inhibitors and precursors, it has been shown that ethylene participates in the regulation of several Fe-deficiency stress responses by Strategy I plants, such as enhanced ferric reductase activity, rhizosphere acidification, and subapical root hair development. Furthermore, recent evidence suggests that ethylene could regulate the expression of both the ferric reductase and the iron transporter genes of Strategy I plants by affecting the FER (or FER-like) transcription factor. Recently, two H⁺-ATPase genes have been isolated from cucumber roots, CsHA1 and CsHA2. CsHA1 is up-regulated under Fe deficiency while CsHA2 is constitutively expressed. In …

Fertility Restoration Of The Sorghum A3 Male-Sterile Cytoplasm Through A Sporophytic Mechanism Derived From Sudangrass, Hoang V. Tang, Jeffrey F. Pedersen, Christine D. Chase, Daryl R. Pring

Department of Agronomy and Horticulture: Faculty Publications

Fertility restoration of sorghum [Sorghum bicolor (L.) Moench] lines carrying the IS1112C (A3 group) male-sterile cytoplasm has been documented as a two-gene gametophytic mechanism involving complementary action of restoring alleles designated Rf3 and Rf4, as derived from IS1112C. Fertility restoration capability has also been reported from sudangrass (S. bicolor subsp. drummondii) populations. We describe characteristics of a fertility restoration system derived from sudangrass, in which male-sterile individuals were observed at high frequency in backcross and F2-F3 segregating populations. Segregation analyses were consistent with a sporophytic restoration system involving two complementary genes. Pollen iodine staining in fertile …

Array-Based Genotyping And Expression Analysis Of Barley Cv. Maythorpe And Golden Promise, Harkamal Walia, Clyde Wilson, Pascal Condamine, Abdelbagi M. Ismail, Jin Xu, Xinping Cui, Timothy J. Close

Department of Agronomy and Horticulture: Faculty Publications

Background: Golden Promise is a salt-tolerant spring barley closely related to Maythorpe. Salt tolerance in Golden Promise has been attributed to a single mutation at the Ari-e locus (on 5H) resulting from irradiation of Maythorpe. Golden Promise accumulates lower shoot Na⁺ compared to Maythorpe when growing under saline conditions. This study focused on elucidating the genetic basis and mechanisms involved in this difference. Results: The level of polymorphism between the two genotypes was explored using the Barley1 GeneChip for single feature polymorphisms (SFPs) and an oligonucleotide pool assay for single nucleotide polymorphisms (SNPs). Polymorphism analyses revealed three haplotype blocks …

Inoculation Of Coffee Plants With The Fungal Entomopathogen Beauveria Bassiana (Ascomycota: Hypocreales), Francisco Posada, M. Catherine Aime, Stephen W. Peterson, Stephen A. Rehner, Fernando E. Vega

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The entomopathogenic fungus Beauveria bassiana was established in coffee seedlings afterfungal spore suspensions were applied as foliar sprays, stem injections, or soil drenches. Direct injection yielded the highest post-inoculation recovery of endophytic B. bassiana. Establishment, based on percent recovery of B. bassiana, decreased as time post-inoculation increased in all treatments. Several other endophytes were isolated from the seedlings and could have negatively influenced establishment of B. bassiana. The recovery of B. bassiana from sites distant from the point of inoculation indicates that the fungus has thepotential to move throughout the plant.

Nutrient Use Efficiency: Measurement And Management, Achim Dobermann

Department of Agronomy and Horticulture: Faculty Publications

Mineral fertilizers have sustained world agriculture and thus global population and wealth growth for more than 100 years (Smil, 2001; Stewart et al., 2005). Their contribution to increasing crop yields has spared millions of hectares of natural ecosystems that otherwise would have been converted to agriculture (Balmford et al., 2005). However, lacking, imbalanced, inappropriate or excessive use of nutrients in agricultural systems remains a concern. Nutrient mining is a major cause for low crop yields in parts of the developing world, particularly Africa. In other situations, nutrients such as nitrogen (N) and phosphorus (P) often move beyond the bounds of …

Orchard Pollination In Capitol Reef National Park, Utah, Usa. Honey Bees Or Native Bees?, Vincent J. Tepedino, Terry L. Griswold, Diane Gail Alston, Trent R. Toler, Brosi A. Bradley

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Capitol Reef National Park in central Utah, USA surrounds 22 managed fruit orchards started over a century ago by Mormon pioneers. Honey bees are imported for pollination, although the area in which the Park is embedded has over 700 species of native bees, many of which are potential orchard pollinators. We studied the visitation of native bees to apple, pear, apricot, and sweet cherry over 2 years. Thirty species of bees visited the flowers but, except for pear flowers, most were uncommon compared to honey bees. Evidence that honey bees prevented native bees from foraging on orchard crop flowers was …

A Look At The Engineering Challenges Of The Usda Small Watershed Program, G. J. Hanson, L. Caldwell, M. Lobrecht, D. Mccook, S. L. Hunt, D. Temple

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Small Watershed Program, administered by the USDA Natural Resources Conservation Service (formerly the Soil Conservation Service), originated in the 1940s and 1950s through the following statutes: the Flood Control Act of 1944, the pilot watershed program (1953-1954), and the Watershed Protection and Flood Prevention Act of 1954. The Small Watershed Program has been recognized by ASABE as one of the outstanding achievements of agricultural engineering in the 20th century in soil and water. With a $15 billion infrastructure investment, more than 11,000 flood-control dams were constructed, and thousands of acres of farms and ranches are protected by conservation practices. …

Suitability Of Swat For The Conservation Effects Assessment Project: Comparison On Usda Agricultural Research Service Watersheds, Michael W. Van Liew, Tamie L. Veith, David D. Bosch, Jeffrey G. Arnold

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Recent interest in tracking environmental benefits of conservation practices on agricultural watersheds throughout the United States has led to the development of the United States Department of Agriculture’s (USDA) Conservation Effects Assessment Project (CEAP). The purpose of CEAP is to assess environmental benefits derived from implementing various USDA conservation programs for cultivated, range, and irrigated lands. Watershed scale, hydrologic simulation models such as the Soil and Water Assessment Tool (SWAT) will be used to relate principal source areas of contaminants to transport paths and processes under a range in climatic, soils, topographic, and land use conditions on agricultural watersheds. To …

Effects Of Cropland Conservation Practices On Fish And Wildlife Habitat, Stephen J. Brady

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A literature review of commonly applied cropland soil and water conservation practices and their impact on fish and wildlife habitat is presented. Agriculture has had the most extensive effect on wildlife habitat of any human-induced factor in the United States. Any practice that improves runoff water quality and/or reduces sediment delivery will have beneficial effects to aquatic ecosystems. Many soil and water conservation practices have additional benefits to wildlife when applied in a habitat-friendly manner, but may have little or no benefit when applied otherwise. Wildlife and agriculture can coexist if land is managed to conserve sufficient biological integrity in …

Effects Of Conservation Practices On Aquatic Habitats And Fauna, Scott Knight, Kathryn Boyer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A major goal of both state and federal agricultural and environmental agencies in the United States is sustainable management of watersheds where agriculture is a dominant land use. Because watershed processes and conditions directly and indirectly affect soil, water, air, plants, animals, and humans, USDA NRCS encourages a watershed approach to management of agricultural operations in the United States. This requires a suite of approaches or practices that address natural resource concerns in uplands and stream corridors. Land clearing, leveling, draining, tilling, fertilizing, and harvesting together create prolonged perturbations manifested in the ecological and physical conditions of streams and rivers. …

Construction And Testing Of A Simple And Economical Soil Greenhouse Gas Automatic Sampler, Daniel Ginting, Spemcer L. Arnold, Nicholas S. Arnold, Ronald S. Tubbs

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Quantification of soil greenhouse gas emissions requires considerable sampling to account for spatial and/or temporal variation.With manual sampling, additional personnel are often not available to sample multiple sites within a narrow time interval. The objectives were to construct an automatic gas sampler and to compare the accuracy and precision of automatic versus manual sampling. The automatic sampler was tested with carbon dioxide (CO₂) fluxes that mimicked the range of CO₂ fluxes during a typical corn-growing season in eastern Nebraska. Gas samples were drawn from the chamber at 0, 5, and 10 min manually and with the automatic …

Long-Term Application Of Biosolids On Apricot Production, Gary S. Ban˜Uelos, Sajeemas Pasakdee, Sharon E. Benes, Craig R. Ledbetter

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The use and disposal of biosolids, or wastewater treatment sludge, as a fertilizer and soil amendment is becoming increasingly widespread. We evaluated the multiyear use of biosolids in apricot (Prunus armeniaca L.) production, grown on productive agricultural soils. Class A biosolids were initially applied annually at rates of 0, 1.9, 5.8, and 11.7 Mg · ha^-1 (dry basis) to a 2-year-old apricot orchard on the USDAARS research site on the eastern side of the San Joaquin Valley, CA. These application rates provided estimated rates of 0 (control), 57, 170, and 340 kg total N · ha^-1 yr …

Estimates Of Gross Transformation Rates Of Dairy Manure N Using 15N Pool Dilution, T. S. Griffin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Most measurements of dairy manure nitrogen (N) availability depend on net
changes in soil inorganic N concentration over time, which overlooks the cycling of manure N in the soil. Gross transformations of manure N, including mineralization (m), immobilization (i), and nitrification (n), can be quantified using ¹⁵N pool dilution methods. This research measures gross m, n, and i resulting from application of four freeze-dried dairy manures that had distinctly different patterns of N availability. A sandy loam soil (coarse-loamy, mixed, frigid Typic Haplorthod) was amended with four different freeze-dried dairy manures and …

A Comparison Of Pollen Counts: Light Versus Scanning Electron Microscopy, Gretchen D. Jones, Vaughn M. Bryant Jr.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Palynologists use compound light microscopy (LM) for pollen identification and interpretation and scanning electron microscopy (SEM) for morphological comparisons and taxonomy. As we are unaware of any published reports comparing LM and SEM pollen counts and identifications of the same sample, we decided to examine a surface soil sample using both microscopes. Standard palynological extraction techniques were used. Two, 300 grain counts were made using LM, and two, 300 counts with SEM. Pollen grains viewed with SEM were also divided into three categories, ‘‘identifiable,’’ ‘‘obscured,’’ and ‘‘virtually impossible to identify’’. Eighty-six (86) percent of the pollen grains counted with SEM …

Compost And Manure Effects On Fertilized Corn Silage Yield And Nitrogen Uptake Under Irrigation, Gary A. Lehrsch, D. C. Kincaid

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Dairy manure increases the yields of dry bean (Phaseolus vulgaris L.) and spring wheat (Triticum aestivum L.) from eroded, furrow-irrigated soils and may increase corn (Zea mays L.) silage yield from steeper eroded areas under sprinkler irrigation. In a 2-year field study in southern Idaho on Portneuf silt loam (coarse silty, mixed, superactive, mesic Durinodic Xeric Haplocalcid), the effects of a one-time, fall application of 29 or 72 Mg ha^-1 of dry manure or 22 or 47 Mg ha^-1 of dry compost on subsequent silage yield and nitrogen (N) uptake from previously eroded, sprinkler-irrigated hill …

Spectral Analysis Of Coastal Vegetation And Land Cover Using Aisa+ Hyperspectral Data, R. Jensen, P. Mausel, N. Dias, R. Gonser, C. Yang, J. Everitt, R. Fletcher

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This paper describes a spectral analysis of several coastal land cover types in
South Padre Island, Texas using AISA₊ hyperspectral remote sensing data.
AISA₊ hyperspectral data (1.5 metre) were acquired throughout the area on 9
March 2005. Data over mangrove areas were converted to percent reflectance
using four 8 ×8 metre reflectance tarps (4%, 16%, 32% and 48%) and empirical
line calibration. These data were then compared to percent reflectance values of
other terrestrial features to determine the ability of AISA₊ data to distinguish
features in coastal environments. Results suggest that these data may be
appropriate to …

Morphological And Histochemical Characterization Of The Seminiferous Epithelial And Leydig Cells Of The Turkey, M. R. Bakst, V. Akuffo, P. Trefil, J. P. Brillard

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Unlike mammals, there is little fundamental information about spermatogenesis in birds. This study was undertaken to clarify the morphology, histochemistry, and lectin affinity of the seminiferous epithelial cells and Leydig cells in pre-pubertal (8- to 15-week old) and adult (40- to 44-week old) domestic turkeys. In adult turkeys, three types of spermatogonia were defined based on their chromatin distribution and nuclear morphology: the dark type A(A_d); the pale type A(A_p); and the type B. The A_d is the least numerous and least conspicuous and consequently difficult to locate. Based on its spatial distribution and overall …

Effect Of Age And Mating Status On Adult European Corn Borer (Lepidoptera: Crambidae) Dispersal From Small-Grain Aggregation Plots, Brendon J. Reardon, Thomas W. Sappington

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), is often controlled with genetically modified corn, Zea mays L., hybrids (Bacillus thuringiensis [Bt] corn) in the United States. If Bt-resistant insects are detected in the field, mitigation-remediation tactics must be implemented to sustain the efficacy of insecticidal, transgenic corn. Mass releasing laboratory reared, susceptible adults near aggregation sites to mate with locally emerging resistant adults is a possible remediation tactic, but it is imperative that the former remain in or near the release site long enough to mate. Understanding adult dispersal behavior relative to the timing of mating is …

2007 Census Of Agriculture

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Introduction .......... VII

State Map .......... 1

Figures .......... 2-6

Tables

Chapter 1. State Data .......... 7-236

Chapter 2. County Data .......... 249-617

Appendices

Census of Agriculture Methodology .......... A-1

General Explanation and Census of Agriculture Report Form .......... B-1

Index .......... Index 1

Publication Program .......... Inside Back Cover

Occurrence Of The Waxy Alleles Wxa And Wxb In Waxy Sorghum Plant Introductions And Their Effect On Starch Thermal Properties, Jeffrey F. Pedersen, Robert A. Graybosch, D. L. Funnell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The existence of two waxy alleles, wx^a associated with no detectable granule bound starch synthase (GBSS) and wx^b associated with apparently inactive GBSS, was recently reported in sorghum [Sorghum bicolor (L.) Moench]. In this paper, the occurrence of the wx^a and wx^b alleles in the USDA-ARS photoperiod-insensitive sorghum collection was determined, and the effects of the wx^a and wx^balleles on thermal properties of sorghum starch (gelatinization temperatures and energy requirements) measured by differential scanning calorimetry. Of the 51 purported waxy accessions examined, 14 tested positive for presence of amylose by iodine staining and …

Laboratory Comparison Of Aedes Aegypti Attraction To Human Odors And To Synthetic Human Odor Compounds And Blends, Ulrich R. Bernier, Daniel L. Kline, Sandra A. Allan, Donald R. Barnard

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Chemical lures can enhance the ability of traps to collect mosquitoes, selectively target species for capture, and provide a realistic assessment of the species and host-equivalent numbers of mosquitoes present in the local area. One approach to the development of chemical lures is to manufacture blends that comprise odors released in human emanations. These blends need to be safe for use in the environment, desirable from an economic standpoint, and transportable to the field for use in traps. In this report, we compared the attraction of mosquitoes to various chemicals, blends, and odors from humans. Noncompetitive (single-treatment) bioassays established that …

Analysis Of Extracted And Volatile Components In Blackstrap Molasses Feed As Candidate House Fly Attractants, Brian Quinn, Ulrich R. Bernier, Christopher J. Geden, Jerome A. Hogsette, David A. Carlson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

House flies are a ubiquitous insect that have the potential to spread many diseases to humans and livestock. Managing house fly populations is accomplished by having desirable baits, traps, and killing agents. Most house fly baits are designed for outdoor use or limited indoor use, and have a foul odor that is not conducive to food preparatory and dining areas. Blackstrap molasses has long been used as a house fly bait, but it is sticky and viscous, making it difficult to handle. This study sought to identify compounds present in blackstrap molasses that might be attractive to house flies, and …

Mosquito And Fly Control Research By The Usda-Ars Center For Medical, Agriculture And Veterinary Entomology (Cmave) In The Deployed War-Fighter Protection (Dwfp) Program, Kenneth J. Linthicum, Sandra A. Allan, Donald Barnard, James Becnel, Ulrich R. Bernier, Seth Britch, Gary Clark, Miriam Cooperband, Chris Geden, Jerome Hogsette, Daniel Kline, Roberto Pereira, Julia Pridgeon, Brian Quinn, Craig Welch, Liming Zhao

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Despite existing measures to prevent and control arthropod-borne diseases in military units, these diseases continue to be serious threats to deployed troops. Due to a shrinking list of safe, cost-effective pesticides for control of disease vectors, new and improved toxicants and methods for delivery are needed by the armed forces. Since 2004, the USDA Agricultural Research Service (ARS) has participated in the Department of Defense (DOD) - sponsored Deployed War-Fighter Protection (DWFP) program to identify and develop new tools for combating pest and vector species that impact deployed war-fighters. Ongoing research at the USDA-ARS Center for Medical, Agricultural and Veterinary …

Isolation Of Salmonella Enterica And Shiga-Toxigenic Escherichia Coli O157 From Feces Of Animals In Public Contact Areas Of United States Zoological Parks, James E. Keen, Lisa M. Durso, Thomas P. Meehan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The fecal prevalence of subclinical Salmonella enterica and Shiga-toxigenic Escherichia coli O157 among animals in human-animal contact exhibits at institutions in the United States accredited by the Association of Zoos and Aquariums was estimated to assess public health risk. The prevalence was less than 0.6% for both zoonotic pathogens among 997 animals sampled at 36 exhibits.

A Cellulose Fiber-Based Diet For Screwworm (Diptera: Calliphoridae) Larvae, M. F. Chaudhury, Steven R. Skoda

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A highly absorbent cellulose fiber from recycled paper was tested and compared with a polyacrylate gelling agent, Aquatain, normally used for bulking and solidifying larval rearing medium of screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). The absorbent fiber, when mixed with water and dietary ingredients, produced a diet medium of homogeneous texture that supported larval growth and development comparable with the standard gelled diet. Larval and pupal weights from two concentrations of cellulose fiber-based diet were significantly higher than those obtained using gelled diet. The number of pupae per tray, percentage of adult emergence, oviposition, percentage of egg hatch, and adult …

Lixus Cardui, A Biological Control Agent For Scotch Thistle (Onopordum Acanthium): Safe For Australia, But Not The Usa?, Joe Balciunas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Invasive exotic plants are often weeds in more than one country. After a biological control agent for a weed has been developed for use in one country, it is reasonable to consider using the same agent against the same weed in another country. ‘Transfer Projects’ can save considerable time and money, and they have been popular around the world. Lixus cardui Olivier (Coleoptera: Curculionidae), a weevil from Europe, was first used by Australian researchers to control Scotch thistle, Onopordum acanthium L. (Asteraceae). There are few close relatives of Scotch thistle in Australia, but that is not the case in North …

Physiological Host Range Of Ceratapion Basicorne, A Prospective Biological Control Agent Of Centaurea Solstitialis (Asteraceae), Lincoln Smith

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ceratapion basicorne (Coleoptera: Apionidae) is a weevil native to Europe and western Asia that is being evaluated as a prospective classical biological control agent of Centaurea solstitialis (yellow starthistle) in the United States. Host plant specificity of the insect was evaluated in no-choice oviposition experiments. Feeding on leaf tissue by adult females was highly correlated to oviposition rate, both of which occurred primarily on plants in the tribe Cardueae, and especially those in the monophyletic subtribe Centaureinae. The highest rates of larval development occurred on Ce. solstitialis and Centaurea cyanus (bachelor’s button, garden cornflower), and there was significant development on …