Agricultural Science Commons^™

Uso De Molinos Cti Para La Produccion Y Uso De Harina De Sorgo, Intsormil

INTSORMIL Impacts and Bulletins

Para ayudar a lidiar con el aumento de los costos de trigo en Centro América, los panaderos están buscando formas alternativas para producir sus productos de panadería a un bajo costo sin sacrificar el valor nutritivo o sabor. La harina de sorgo es la respuesta a sus problemas. La harina de sorgo es de aproximadamente la mitad del costo de la harina de trigo, por lo que usar harina de sorgo puede ayudar a los panaderos a ahorrar dinero y disminuir costos de produccion. El tamaño de partícula de la harina de sorgo debe ser lo suficientemente fino como para …

Comparison Of Whole-Genome Amplifications For Microsatellite Genotyping Of Rotylenchulus Reniformis, R. S. Arias, Salliana R. Stetina, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Currently, a large number of microsatellites are available for Rotylenchulus reniformis (reniform nematode); however, two barriers exist for genotyping samples from different geographical areas. The limited amount of nucleic acids obtained from single nematodes which would require their multiplication to obtain enough DNA for testing; and the strictly regulated transport of live samples and multiplication in greenhouse for being a plant pathogen. Whole-genome amplification (WGA) of samples consisting of one and five dead gravid females with their associated egg masses was successfully performed on disrupted tissue using three commercial kits. DNA yield after WGA ranged from 0.5 to 8 μg …

First Microsatellites From Spodoptera Frugiperda (Lepidoptera: Noctuidae) And Their Potential Use For Population Genetics, R. S. Arias, Carlos A. Blanco, Maribel Portilla, Gordon L. Snodgrass, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This is the first report of sequence-specific microsatellite markers (simple sequence repeats [SSRs]) of fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), an economically important pest of crops on the Americas.Weisolated 192 microsatellite markers by using pyrosequencing and screened 15 individuals from eight isofamilies collected from three geographical areas: Puerto Rico (PR), Texas (TX), and Mississippi (MS). Isofamilies resistant to Cry toxins from Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) also were included. Cluster analysis was performed to determine the potential use of these SSRs in discriminating populations, and colonies were grouped with a reliability of 100% estimated by bootstrap. In this …

Screening Synteny Blocks In Pairwise Genome Comparisons Through Integer Programming, Haibao Tang, Eric Lyons, Brent S. Pedersen, James C. Schnable, Andrew H. Paterson, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Background:

It is difficult to accurately interpret chromosomal correspondences such as true orthology and paralogy due to significant divergence of genomes from a common ancestor. Analyses are particularly problematic among lineages that have repeatedly experienced whole genome duplication (WGD) events. To compare multiple “subgenomes” derived from genome duplications, we need to relax the traditional requirements of “one-to-one” syntenic matchings of genomic regions in order to reflect “one-to-many” or more generally “many-to-many” matchings. However this relaxation may result in the identification of synteny blocks that are derived from ancient shared WGDs that are not of interest. For many downstream analyses, we …

Malian Thick Porridges And Satiety, Intsormil

INTSORMIL Impacts and Bulletins

A study was recently conducted through INTSORMIL (Purdue Hamaker Project) to examine thick sorghum millet consumption related to preference and satiation in the Sikasso, Segou and Mopti regions of Mali. This is part of a larger study to understand the effect of thick porridges, and delayed glucose delivery to the body, on satiety and overall food consumption.

The conclusions from the following data is that porridges (tô) are generally eaten more frequently and are consumed in a thicker consistency in the villages (Figures 1 and 2), and are very satiating (Figure 3 thicker porridges correlated with lower hunger scores at …

A First Insight Into Population Structure And Linkage Disequilibrium In The U.S. Peanut Minicore Collection, Vikas Belamkar, Michael Gomez Selvaraj, Jamie L. Ayers, Paxton R. Payton, Naveen Puppala, Mark D. Burow

Department of Agronomy and Horticulture: Faculty Publications

Knowledge of genetic diversity, population structure, and degree of linkage disequilibrium (LD) in target association mapping populations is of great importance and is a prerequisite for LD-based mapping. In the present study, 96 genotypes comprising 92 accessions of the US peanut minicore collection, a component line of the tetraploid variety Florunner, diploid progenitors A. duranensis (AA) and A. ipaënsis (BB), and synthetic amphidiploid accession TxAG-6 were investigated with 392 simple sequence repeat (SSR) marker bands amplified using 32 highly-polymorphic SSR primer pairs. Both distance- and model-based (Bayesian) cluster analysis revealed the presence of structured diversity. In general, the wild-species accessions …

Genes Identified By Visible Mutant Phenotypes Show Increased Bias Toward One Of Two Subgenomes Of Maize, James C. Schnable, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Not all genes are created equal. Despite being supported by sequence conservation and expression data, knockout homozygotes of many genes show no visible effects, at least under laboratory conditions. We have identified a set of maize (Zea mays L.) genes which have been the subject of a disproportionate share of publications recorded at MaizeGDB. We manually anchored these ‘‘classical’’ maize genes to gene models in the B73 reference genome, and identified syntenic orthologs in other grass genomes. In addition to proofing the most recent version 2 maize gene models, we show that a subset of these genes, those that …

Dose–Sensitivity, Conserved Non-Coding Sequences, And Duplicate Gene Retention Through Multiple Tetraploidies In The Grasses, James C. Schnable, Brent S. Pedersen, Sabarinath Subramaniam, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Whole genome duplications, or tetraploidies, are an important source of increased gene content. Following whole genome duplication, duplicate copies of many genes are lost from the genome. This loss of genes is biased both in the classes of genes deleted and the subgenome from which they are lost. Many or all classes are genes preferentially retained as duplicate copies are engaged in dose sensitive protein–protein interactions, such that deletion of any one duplicate upsets the status quo of subunit concentrations, and presumably lowers fitness as a result. Transcription factors are also preferentially retained following every whole genome duplications studied. This …

Inta Segovia, A New Sorghum Variety For The Dry Zones Of Nicaragua, Intsormil

INTSORMIL Impacts and Bulletins

The Sandanista Government through the Instituto Nicaraguense de Tecnologia (INTA) officially released the new improved sorghum variety, INTA Segovia on January 14, 2011. This improved variety will benefit the resource-poor farmers that grow sorghum on the hillsides (see photo) in dry zones (40 percent of the land area in Nicaragua). This variety will have a significant economic impact in Nicaragua and in neighboring countries as it produces well in drought prone areas which lack sufficient rainfall for growing maize. INTA Segovia, under drought conditions, produces higher yields than currently grown sorghum varieties. Drought tolerance is due to the large root …

Proceedings Of The 38th Annual Meeting, Southern Soybean Disease Workers (March 9-10, 2011, Pensacola Beach, Florida), Boyd Padgett, Tom Allen, Jason Bond, Cliff Coker, Stephen R. Koenning

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern United States Soybean Disease Loss Estimates for 2010. Compiled by SR Koenning

Poster presentation

Soybean Varieties and Breeding Lines Shown With Resistance to Reniform Nematode in Greenhouse Tests, 1998-2010 R. T. Robbins, and L. E. Jackson

Oral presentations

Frogeye Leaf Spot (Cercorosa sojina) symposium (Boyd Padgett, moderator)

Development of Baseline Cercospora sojina Sensitivity Levels to Quinone Outside Inhibitor Fungicides and Monitoring for Resistance. GR Zhang and CA Bradley

Soybean Pathogen Found to be Resistant to Fungicides MA Newman and CA Bradley

Strobilurin-resistant Cercospora sojina in Kentucky: Field History. D Hershman

Graduate student paper competition (Tom Allen, …

Comparison Of Various Configurations Of Cdc-Type Traps For The Collection Of Phlebotomus Papatasi Scopoli In Southern Israel, Daniel L. Kline, Jerome Hogsette, Gunter Muller

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We conducted two experiments to determine the best CDC-trap configuration for catching male and female Phlebotomus papatasi. First, visual features were evaluated. Standard CDC traps were modified to have black or white catch bags, black or white lids, or no lids and these were tried in different combinations. Significantly more male sand flies were caught by darker traps; significantly more females were captured by traps with either all black or a combination of black and white features. Attraction may be due to dark color or contrast in colors. CDC traps with suction and the following features were also evaluated: …

Instalan Molino Para Producir Harina De Sorgo: Con Este Molino Se Prevé Procesar, Al Mes, Alrededor De 48 Quintales De Harina, Berta Nely Menjívar

INTSORMIL Impacts and Bulletins

Panificadores de la Asociación Cooperativa de Cuscatlán (ACPACUS de R. L.), aglutinados en la Mesa Nacional de Panificadores de El Salvador (MENAPAES), en compañía de autoridades del Centro Nacional de Tecnología Agropecuaria y Forestal Enrique Álvarez Córdova (CENTA) y del Instituto para la Investigación del Sorgo y Mijo (INTSORMIL), inauguraron la puesta en marcha del primer molino de martillo, con el cual estarán procesando harina de sorgo (maicillo) para utilizarla en la industria de la panificación.

La adquisición de este molino es uno de los primeros esfuerzos de la MENAPAES en la búsqueda de otras materias primas para la producción …

Sorghum And Millet Research Program Receives Grant, Daily Nebraskan

INTSORMIL Impacts and Bulletins

The International Sorghum and Millet Collaborative Research Support Program (INTSORMIL) at the University of Nebraska-Lincoln recently received a $1.1 million grant to work with Central American countries to increase sorghum foliage.

Intsormil Information, Daily Nebraskan

INTSORMIL Impacts and Bulletins

The International Sorghum and Millet Collaborative Research Support Program (INTSORMIL) at the University of Nebraska-Lincoln recently received at $1.1 million grant to work with Central American countries to increase sorghum foliage.

Report Of Nine Sorghum Flour Workshops, Report For Intsormil; Subject: “Utilization Of Cti Grinders For Small Scale Sorghum Flour Production In Central America”, Intsormil

INTSORMIL Scientific Publications

In Nicaragua, Sorghum grain has been a crops using only for animal feed. Fortunately Scientifics have been researched about its nutritional sorghum grain properties and some of the country that they have been interested to bring the technology about sorghum flour has been Nicaragua because its growths in 3 big areas; in the north, south and west region. Now, that is knew their characteristics and others, so INTA and INTSORMIL are diffusing the technology about sorghum flour to increase the production of sorghum grain, reduce the cost production of bread and some ethnics drinks, to improve the food security and …

Switchgrass, Kenneth P. Vogel, Gautam Sarath, Aaron J. Saathoff, Robert B. Mitchell

Department of Agronomy and Horticulture: Faculty Publications

17.1 Introduction

Switchgrass (Panicum virgatum L.) is a warm-season perennial grass that is native to North America that is being developed into a biomass energy crop. It has been used in pastures and for conservation purposes in the Great Plains and the Midwest, USA, for over 70 years.1 The research supporting its use as a pasture and conservation species was largely conducted by US Department of Agriculture (USDA) research programs, most notably the Agricultural Research Service (ARS) project located at the University of Nebraska-Lincoln and USDA Plant Materials Centers that are located throughout the United States. In this report, …

Identification Of A Second Asian Soybean Rust Resistance Gene In Hyuuga Soybean, Mandy D. Kendrick, Donna K. Harris, Bo-Keun Ha, D. L. Hyten, P. B. Cregan, Reid D. Frederick, H. Roger Boerma, Kerry F. Pedley

Department of Agronomy and Horticulture: Faculty Publications

Asian soybean rust (ASR) is an economically significant disease caused by the fungus Phakopsora pachyrhizi. The soybean genes Rpp3 and Rpp?(Hyuuga) confer resistance to specific isolates of the pathogen. Both genes map to chromosome 6 (Gm06) (linkage group [LG] C2). We recently identified 12 additional soybean accessions that harbor ASR resistance mapping to Gm06, within 5 centimorgans of Rpp3 and Rpp?(Hyuuga). To further characterize genotypes with resistance on Gm06, we used a set of eight P. pachyrhizi isolates collected from geographically diverse areas to inoculate plants and evaluate them for differential phenotypic responses. …

Mutational Analysis Of The Major Soybean Uref Paralogue Involved In Urease Activation, Joe C. Polacco, D. L. Hyten, Mônica Medeiros-Silva, David A. Sleper, Kristin D. Bilyeu

Department of Agronomy and Horticulture: Faculty Publications

The soybean genome duplicated ~14 and 45 million years ago and has many paralogous genes, including those in urease activation (emplacement of Ni and CO₂ in the active site). Activation requires the UreD and UreF proteins, each encoded by two paralogues. UreG, a third essential activation protein, is encoded by the single-copy Eu3, and eu3 mutants lack activity of both urease isozymes. eu2 has the same urease-negative phenotype, consistent with Eu2 being a single-copy gene, possibly encoding a Ni carrier. Unexpectedly, two eu2 alleles co-segregated with missense mutations in the chromosome 2 UreF paralogue (Ch02UreF), suggesting …

Cytokinin-Mediated Source ⁄Sink Modifications Improve Drought Tolerance And Increase Grain Yield In Rice Under Water-Stress, Zvi Peleg, Maria Reguera, Ellen Tumimbang, Harkamal Walia, Eduardo Blumwald

Department of Agronomy and Horticulture: Faculty Publications

Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isopentenyltransferase (IPT) gene driven by PSARK, a stress- and maturation-induced promoter. Plants were tested for drought tolerance at two yield-sensitive developmental stages: pre- and post-anthesis. Under both treatments, the transgenic rice plants exhibited delayed response to stress with significantly higher grain yield (GY) when compared to wild-type plants. Gene expression analysis revealed a significant shift in expression of hormone-associated …

Evaluation Of Hha And Hha Sepb Mutant Strains Of Escherichia Coli O157:H7 As Bacterins For Reducing E. Coli O157:H7 Shedding In Cattle, Vijay K. Sharma, Evelyn A. Dean-Nystrom, Thomas A. Casey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Escherichia coli O157:H7 colonizes cattle intestines by using the locus of enterocyte effacement (LEE)- encoded proteins. The induction of systemic immune response against LEE-encoded proteins, therefore, will prove effective in reducing E. coli O157:H7 colonization in cattle. The previous studies have demonstrated that a hha (encodes for a hemolysin expression modulating protein) deletion enhances expression of LEE-encoded proteins and a sepB (encodes an ATPase required for the secretion of LEE-encoded proteins) deletion results in intracellular accumulation of LEE proteins. In this study, we demonstrate the efficacy of the hha and hha sepB deletion mutants as bacterins for …

The Ability Of Selected Pupal Parasitoids (Hymenoptera: Pteromalidae) To Locate Stable Fly Hosts In A Soiled Equine Bedding Substrate, Jimmy B. Pitzer, Phillip E. Kaufman, Christopher Geden, Jerome A. Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The ability of Spalangia cameroni Perkins, Spalangia endius Walker, and Muscidifurax raptorellus Kogan and Legner to locate and attack stable fly hosts was evaluated under laboratory conditions. Postfeeding third-instar stable fly larvae were released and allowed to pupate in two arena types: large 4.8 liter chambers containing a field-collected, soiled equine bedding substrate; or 120-ml plastic cups containing wood chips. At the time of fly pupariation, parasitoids were released and permitted 72 h to locate and attack hosts. On average, parasitism rates of freely accessible stable fly pupae in cups were not significantly different between parasitoid species. However, parasitism rates …

New Records For The Horse Fly Fauna (Diptera: Tabanidae) Of Jordan With Remarks On Ecology And Zoogeography, Günter Müller, Jerome Hogsette, Edita Revay, Vasiliy Kravchenko, Andrey Leshvanov, Yosef Schlein

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The horse fly fauna (Diptera: Tabanidae) of Jordan is, after Israel, the richest in the Levant, with 24 known species. During the 20-year project “The Ecology and Zoogeography of the Lepidoptera of the Near East,” we regularly collected blood-feeding flies, resulting in 11 additional species of Tabanidae for Jordan. The new records are: Atylotus quadrifarius (Loew, 1874), Chrysops caecutiens (Linnaeus, 1758), Dasyrhamphis nigritus (Fabricius, 1794), Haematopota pallens Loew, 1871, Nemorius irritans (Ricardo, 1901), Philipomyia graeca (Fabricius, 1794), Tabanus cordiger Meigen, 1820, Tabanus taeniola Palisot de Beauvois, 1806, Tabanus quatuornotatus Meigen, 1820, Tabanus separatus Effllatoun, 1930, and Tabanus spectabilis Loew, 1858. …

Vector Competence Of The Stable Fly (Diptera: Muscidae) For West Nile Virus, Michael S. Doyle, Bethany N. Swope, Jerome A. Hogsette, Kristen L. Burkhalter, Harry M. Savage, Roger S. Nasci

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In 2006-2007, stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae), were suspected of being enzootic vectors of West Nile virus (family Flaviviridae, genus Flavivirus, WNV) during a die-off of American white pelicans (Pelecanus erythrorhynchos Gmelin) (Pelecanidae) in Montana, USA. WNV-positive stable flies were observed feeding en masse on incapacitated, WNV-positive pelicans, arousing suspicions that the flies could have been involved in WNV transmission among pelicans, and perhaps to livestock and humans. We assessed biological transmission by infecting stable flies intrathoracically with WNV and testing them at 2-d intervals over 20 d. Infectious WNV was detected in fly bodies …

Dietary Wheat Germ Oil Influences Gene Expression In Larvae And Eggs Of The Oriental Fruit Fly, Thomas A. Coudron, Chiou Ling Chang, Cynthia L. Goodman, David Stanley

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Changes in animal nutrition, particularly essential dietary components, alter global gene expression patterns. Our goal is to identify molecular markers that serve as early indicators of the quality of insect culture media. Markers of deficient culture media will increase the efficiency of developing optimal systems for mass rearing beneficial insects and some pest species because decisions on culture media quality can be made without waiting through one or several life cycles. The objective of our current study is to discover molecular markers of essential dietary lipid deficiency in the oriental fruit fly, Bactrocera dorsalis. We reared groups of fruit …

Uncertainty In Modelling Of Faecal Coliform Overland Transport Associated With Manure Application In Maryland, A. K. Guber, Y. A. Pachepsky, A. M. Yakirevich, D. R. Shelton, A. M. Sadeghi, D. C. Goodrich, C. L. Unkrich

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Concerns for microbial safety of surface water facilitate development of predictive models that estimate concentrations and total numbers of pathogen and indicator organisms leaving manure-fertilized fields in overland flow during runoff events. Spatial variability of bacterial concentrations in applied manure introduces high uncertainty in the model predictions. The objective of this work was to evaluate the uncertainty in model predictions of the manure-borne bacteria overland transport caused by limited information on the spatial distribution of bacteria in surface-applied manure. Experiments were carried out at the ARS Beltsville experimental watershed site (OPE3) in Maryland. Dairy bovine manure was applied at a …

Conservation Reserve Enhancement Program Nebraska Platte-Republican Resources Area

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Overview

Benefits

Goals

Program Cost

Eligible Areas and Eligibility Requirements

Approved Conservation Practices

Acreage Limitations

Sign-up and Contract Duration

CREP Payments

CREP and CRP

For More Information

Outcrossing And Crossbreeding Recovers Deteriorated Traits In Laboratory Cultured Steinernema Carpocapsae Nematodes, John M. Chaston, Adler R. Dillman, David I. Shapiro-Ilan, Anwar L. Bilgrami, Randy Gaugler, Keith R. Hopper, Byron J. Adams

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The nematode Steinernema carpocapsae infects and kills many pest insects in agro-ecosystems and is commonly used in biocontrol of these pests. Growth of the nematodes prior to distribution for biocontrol commonly results in deterioration of traits that are essential for nematode persistence in field applications. To better understand the mechanisms underlying trait deterioration of the efficacy of natural parasitism in entomopathogenic nematodes, we explored the maintenance of fitness related traits including reproductive capacity, heat tolerance, virulence to insects and ‘tail standing’ (formerly called nictation) among laboratory-cultured lines derived from natural, randomly mating populations of S. carpocapsae. Laboratory cultured nematode …

Carbon Dioxide Fluxes In Corn–Soybean Rotation In The Midwestern U.S.: Inter- And Intra-Annual Variations, And Biophysical Controls, Guillermo Hernandez-Ramirez, Jerry L. Hatfield, Timothy B. Parkin, Thomas J. Sauer, John H. Prueger

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Quantifying carbon dioxide (CO₂) fluxes in terrestrial ecosystems is critical for better understanding of global carbon cycling and observed changes in climate. This study examined year-round temporal variations of CO₂ fluxes in two biennial crop rotations during 4 year of corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] production. We monitored CO₂ fluxes using eddy-covariance (EC) and soil chambers in adjacent production fields near Ames, Iowa. Under the non-limiting soil water availability conditions predominant in these fields, diel and seasonal variations of CO₂ fluxes were mostly controlled by ambient temperature and …

Forage Soybean Yield And Quality Response To Water Use, David C. Nielsen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Forages could be used to diversify reduced and no-till dryland cropping systems from the traditional wheat (Triticum aestivum L.)-fallow system in the semiarid central Great Plains. Forages present an attractive alternative to grain and seed crops because of greater water use efficiency and less susceptibility to potentially devastating yield reductions due to severe water stress during critical growth stages. However, farmers need a simple tool to evaluate forage productivity under widely varying precipitation conditions. The objectives of this study were to (1) quantify the relationship between crop water use and dry matter (DM) yield for soybean (Glycine max …

Evaluating Decision Rules For Dryland Rotation Crop Selection, David C. Nielsen, Merle F. Vigil, Joseph G. Benjamin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

No-till dryland winter wheat (Triticum aestivum L.)-fallow systems in the central Great Plains have more water available for crop production than the traditional conventionally tilled winter wheat-fallow systems because of greater precipitation storage efficiency. That additional water is used most efficiently when a crop is present to transpire the water, and crop yields respond positively to increases in available soil water. The objective of this study was to evaluate yield, water use efficiency (WUE), precipitation use efficiency (PUE), and net returns of cropping systems where crop choice was based on established crop responses to water use while incorporating a …