Agricultural Science Commons^™

Mapping The Fas Locus Controlling Stearic Acid Content In Soybean, M. M. Spencer, V. R. Pantalone, E. J. Meyer, D. Landau-Ellis, D. L. Hyten Jr.

Department of Agronomy and Horticulture: Faculty Publications

Increasing the stearic acid content to improve soybean [Glycine max (L) Merr] oil quality is a desirable breeding objective for food-processing applications. Although a saturated fatty acid, stearic acid has been shown to reduce total levels of blood cholesterol and offers the potential for the production of solid fat products (such as margarine) without hydrogenation. This would result in the reduction of the level of trans fat in food products and alleviate some current health concerns. A segregating F₂ population was developed from the cross between Dare, a normal stearic acid content cultivar, and FAM94-41, a high stearic …

Single-Nucleotide Polymorphisms In Soybean, Y. L. Zhu, Q. J. Song, D. L. Hyten, C. P. Van Tassel, L. K. Matukumalli, D. R. Grimm, S. M. Hyatt, E. W. Fickus, N. D. Young, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Single-nucleotide polymorphisms (SNPs) provide an abundant source of DNA polymorphisms in a number of eukaryotic species. Information on the frequency, nature, and distribution of SNPs in plant genomes is limited. Thus, our objectives were (1) to determine SNP frequency in coding and noncoding soybean (Glycine max L. Merr.) DNA sequence amplified from genomic DNA using PCR primers designed to complete genes, cDNAs, and random genomic sequence; (2) to characterize haplotype variation in these sequences; and (3) to provide initial estimates of linkage disequilibrium (LD) in soybean. Approximately 28.7 kbp of coding sequence, 37.9 kbp of noncoding perigenic DNA, and …

Evaluation Of Cauliflower Varieties, Department Of Agriculture, Western Australia, Horticulture Australia

Horticulture research reports

This document has been prepared from a cauliflower variety evaluation trial conducted at the Manjimup Horticultural Research Institute, South West Highway, Manjimup WA 6258. The results reflect the growth characteristics, yield, quality and density of the cauliflower varieties as recorded at this site during the conditions of the growing period (May 2002 to September 2002).

This report is the thirty-fourth in a series produced from the evaluation of cauliflower and broccoli varieties, conducted at Manjimup Horticultural Research Institute. Cauliflower only has been evaluated in this and in subsequent trials.

The results are provided to allow seed suppliers, seedling nurseries, growers, …

‘Forrest’ Resistance To The Soybean Cyst Nematode Is Bigenic: Saturation Mapping Of The Rhg1 And Rhg4 Loci, K. Meksem, P. Pantazopoulos, V. N. Njiti, D. L. Hyten, P. R. Arelli, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv ‘Forrest’ is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of Forrest × Essex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg …

Western Australian Avocado Industry Survey - 2000, Alec Mccarthy

Horticulture research reports

Key components of the project: The avocado industry in Western Australia has been steadily growing over the past 15 years. During this time there has been no comprehensive survey of the industry to determine its size and production base. This survey aimed to collect information from as many commercial avocado growers as possible about tree numbers, age, yield, markets, growing methods and general industry knowledge.

Industry significance: This survey will provide comprehensive data on the avocado industry that will enable producers, packers, marketeers, investors, etc to have greater confidence in their various decision makings.

Key outcomes: Significant increases in annual …

High-Throughput Genotyping For A Polymorphism Linked To Soybean Cyst Nematode Resistance Gene Rhg4 By Using TaqmanTm Probes, Khalid Meksem, Eliza Ruben, David L. Hyten, Michael E. Schmidt, David A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

An individual soybean breeder can generate over one hundred thousand new genotypes each year. The efficiency of selection in these populations could be improved if these genotypes were effectively screened with one DNA marker that identified an important gene, and if laboratory throughput was high and costs were low. Our aim was to develop a rapid genotyping procedure for resistance to the soybean cyst nematode. A high-throughput genotyping method was developed with fluorogenic probes to distinguish between two insertion polymorphisms in alleles of an AFLP marker that is located about 50 kbp from the Rhg4 gene candidate. The assay uses …

Conversion Of Aflp Bands Into High-Throughput Dna Markers, K. Meksem, E. Ruben, D. L. Hyten, K. Triwitayakorn, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

The conversion of AFLP bands into polymorphic sequence-tagged-site (STS) markers is necessary for high-throughput genotype scoring. Technical hurdles that must be overcome arise from genome complexity (particularly sequence duplication), from the low-molecular- weight nature of the AFLP bands and from the location of the polymorphism within the AFLP band. We generated six STS markers from ten AFLP bands (four AFLPs were from co-dominant pairs of bands) in soybean (Glycine max). The markers were all linked to one of two loci, rhg1 on linkage group G and Rhg4 on linkage group A2, that confer resistance to the soybean cyst nematode (Heterodera …

Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Large DNA insert libraries in binary T-DNA vectors can assist in the isolation of the gene(s) under-lying a quantitative trait locus (QTL). Binary vectors facilitate the transfer of large-insert DNA fragments containing a QTL from E. coli to Agrobacterium sp. and then to plants. We constructed two soybean large-insert libraries from cv. Forrest in the pCLD04541 (V41) binary vector after partial digestion of genomic high-molecular-weight DNA with BamHI or HindIII. The libraries contain 76,800 clones with an average insert size of 125 kb, and therefore represent 9.5-fold haploid genome equivalents. Colony hybridization using a chloroplast-specific probe infers that …

Commercial Irrigation And Nutrition Practices For Strawberries In Western Australia, D. Phillips, D. Gatter, T. Calder, D. Pitman

Horticulture research reports

Irrigation practices employed by five commercial strawberry growers on sandy soils of the Swan Coastal Plain near Perth Western Australia were monitored for two consecutive seasons, 1995 and 1996 during the Spring production period.

Nutritional practices for the same grower group plus one Albany grower were monitored by plant, soil and sap analysis at 14 day intervals throughout the 1996 season.

Irrigation records showed that market quality strawberries could be produced at this time of year (August to December in Perth) on soils with very poor water retention properties with an average crop factor(E Pan) for the season of 0.7 …

The Wheat Book : Principles And Practice, W K. Anderson, J R. Garlinge

Bulletins 4000 -

Contents : Environment / revised by J. Cramb, J. Courtney and P. Tille - The structure and development of the cereal plant / revised by T.L. Setter and G. Carlton - Germination, vegetative and reproductive growth / revised by T.L. Setter and G. Carlton - Crop water use / D. Tennant - Nutrition / revised by M.D.A. Bolland, R.F. Brennan, J.W. Bowden, M.G. Mason, N.K. Edwards, M.M. Riley and S.W. Gartrell - Wheat in farming systems / revised by B. Bowden, P. Blackwell, P. Carmody, M. Ewing, R. Kingwell, R. L ghman, I. McFarlane, P. Michael, P. Nelson, I. Pritchard, …

Basic Biotechnology, Jennifer Flak, Julie Albrecht

Plant and Soil Sciences eLibrary

An introduction to traditional cross-breeding and genetic engineering of plants, and to biotechnology.

Objectives - Basic Biotechnology

After completing Nutrition Basic Biotechnology, participants should be able to:

• Describe one similarity between traditional cross-breeding and genetic engineering of plants.
• Give two limitations that are encountered with traditional cross-breeding of plants.
• Explain why genetic engineering is not limited in the same way as traditional cross-breeding.
• Define biotechnology in broad terms.
• List three plant applications of biotechnology.
• List the three areas of nutritional profiles that have been improved with biotechnology.

Modules:

• Lesson home
• Objectives - Basic Biotechnology
• Historical Uses of Biotechnology
• Basic Understanding …

Biotechnology In Western Australian Agriculture, Agriculture Western Australia

Grain and other field crops published reports

The Government of Western Australia believes that the strategic use of biotechnology and particularly gene technology has the potential to offer Western Australian agriculture significant economic benefits by improving the productivity and quality of products, as well as contributing to the development of sustainable farming systems.

Information contained in these binders is intended to give an overview of Biotechnology in relation to what is occurring in Australia, in Western Australia and specifically, at Agriculture Western Australia (AGWEST).

Biotechnology has been around for twenty-five years or more, but has only recently become a public issue in Australia - particularly in relation …

Agriculture Western Australia's Centre For Cropping Systems - Northam, Agriculture Western Australia

Grain and other field crops published reports

Agriculture Western Australia's Centre for Cropping Systems in York Road, Northam, was established as a centre of excellence for research, development and extension into cropping systems.

Its role is to stimulate the contribution of ideas from individuals, groups and organisations with an interest in research, development, and extension relating to innovative, profitable and sustainable crop-based farming systems.

The Centre for Cropping Systems is part of a regional network with strong links to Agriculture Western Australia's Crop Improvement Institute based at South Perth, the Dryland Research Institute at Merredin and the Avondale Research Fann at Beverley. It also links with universities …

Horticulture Research Report 1994 - 1998, Frank Wise Institute, Kununurra, Department Of Agriculture, Western Australia, D. Robinson, M. Howes, R. Randell

Horticulture research reports

This report summarises horticultural research, development and extension activities undertaken by staff from the Kununurra District Office during the period 1993 to 1998 and reported annually at the Frank Wise Institute Horticultural Research Review. The range and extent of activities presented and supported by Agriculture Western Australia with industry collaboration indicates the importance of horticulture in the economies of the Kimberley Region and Western Australia. Further support by funding bodies and agribusiness, and collaboration with other research organizations is also acknowledged. Since the early 1980's, horticultural output and value of production from the Ord River Irrigation Area (ORIA) have risen …

Effects Of Tactical Responses And Risk Aversion On Farm Wheat Supply, Ross S. Kingwell

Natural Resources Research Articles

A discrete stochastic programming model of the farming system of the eastern wheatbelt of Western Australia is used to examine the effect of tactical responses and risk aversion on wheat supply. Including within-season tactical changes to wheat areas decreases the own-price elasticity of supply. By contrast, introducing risk aversion has no consistent effect on the own-price elasticity of supply. The implications for supply models are discussed.

Adaptation Of Lentil In Western Australia: Germplasm Evaluation In 1994, A. Sarker, K. H.M. Siddique, S P. Loss, Co-Operative Research Centre For Legumes In Mediterranean Agriculture, Department Of Agriculture Western Australia

Grain and other field crops published reports

Lentil is one of the grain legumes with potential for inclusion into Australian farming systems on fine textured, neutral to alkaline soils. It is an important protein source for the large populations of the Indian sub-continent and elsewhere, and the export market potential is excellent. Old Canadian varieties grown on a small scale in Victoria (Laird and Callisto) are poorly adapted to short season WA environments, A number of varieties have recently been selected from ICARDA material and released in Victoria and SA (Digger, Cobber, Matilda and Aldinga), and these have adequate adaptation to commence a lentil industry in WA. …

Grain Legume And Canola Autumn Updates 1995, David Williams

Grain and other field crops published reports

Grain legumes

- Chickpea

- Field pea

- Lentil

- Faba beans

Canola essentials:

Site selection -Paddock selection is critical for a successful canola crop. Avoid paddocks with waterlogging, wind erosion and broadleaf weeds - particularly wild radish, turnip, mustard and doublegees.

Soil type - Canela grows well on most soil types, however it prefers well drained gravel soils, red loams, sandy _loams and deeper duplex soils. Soil pH levels (in CaCl_²) of 4.5 to 8.0 are suitable. Soil test paddock for pH, phosphorus, and potassium levels.

Rotation - Grow canola after manipulated pasture, lupins or other grain …

Growing Lettuce, Margaret Graham

Horticulture published reports

The commonly cultivated lettuce, Lactuca sativa, is an annual belonging to the sunflower family. It has been cultivated for over 2500 years, and is a popular salad vegetable.

There are three main subspecies commonly grown.

• IL. sativa ssp capitata - Head type - Crisphead is a lettuce with a firm head and brittle leaves. It is the major lettuce type grown in Australia and is also known as iceberg lettuce. - Butterhead (buttercrunch, mignonette) lettuce has soft, pale leaves and a delicate buttery flavour. It is of major importance in Europe, where it is grown as a greenhouse crop.
• L. …

Gascoyne Research Station Field Day, Department Of Agriculture, Western Australia

Horticulture research reports

Gascoyne Research Station Centenary Open Day Thursday 13 October 1994

100 years (1894-1994) Advancing Agriculture

Welcome to the 1994 Field Day at Gascoyne Research Station. At this day you'll have the opportunity to view and hear discussions of some of the latest Research and Development initiatives in horticulture in the region. We will commence activities at the Packing Shed at 3:00 p.m. with introductions and short discussions of new work which is currently in progress. This will be followed by field talks at the trial sites, as listed in this brochure. We are also pleased to include presentations by leaders …

Bulletin 4256 - Pecan Nut Cultivation, Aldo Labrosciano

Bulletins 4000 -

Pecans Carya illinoensis, an indigenous species to Northern America, are successfully grown between the latitudes of 25° and 35°. There is considerable variation in size and flavour of the 1000 or more varieties of the nuts.

Large scale production is concentrated in north America, Israel and South Africa. Plantings in Australia are in Victoria and New South Wales, and are developing slowly in Western Australia.

Pecans are well suited to Western Australian conditions where the soil is well drained. Though the trees are deciduous, they have no chill requirement and will flourish if water is not limiting.

Visions For Agriculture, Department Of Agriculture, Western Australia

Agriculture reports

This workshop grew out of a conversation between Maurice Barnes, a Trayning farmer and member of the Research Advisory Committee of the Dryland Research Institute, and Steve Porritt, the officer in charge of the Dryland Research Institute at Merredin. Maurice was interested in the idea of posing the question 'What would agriculture be like if we had known as much about this landscape in 1829 as we know now?', to a group of farmers and others interested in the central wheatbelt and its future. Maurice saw this question as a first step toward achieving some shared vision for the future …

1991 Catalogue Of The Australian Lupin Collection Including Field Evaluation Data For Wild, Semi-Domesticated And Fully Domesticated Accessions, J. C. Clements, W. A. Cowling

Grain and other field crops research reports

This report lists all wild, semi-domesticated and fully domesticated accessions in the Australian Lupin Collection to 1991. The Collection is composed of 2,264 accessions (Perth Numbers between 20629 and 27067) introduced to Australia or from naturalized populations and breeding programmes within Australia. Several accessions are selections from variable populations which were identified during field trials in South Perth, Western Australia. This catalogue lists the characteristics and field evaluation data for every accession where available. Plant breeders and crop scientists will find this information useful for the selection of lupin genotypes with desired characteristics.

Section A of this catalogue should be …

Timing Of Nitrogen Fertilizer Application For Cereals, M G. Mason

Division of Plant Research : Technical Report Series

Results are presented for 206 trials carried out between 1959 and 1990, investigating the effect of time of application of nitrogen fertilizers on cereal grain yields. This set of data is the basis of recommendations made for timing of nitrogen fertilizer application for cereals in Western Australia. Attention is drawn to the variability of results obtained in different seasons and situations and the time of leaching rains.

Bulletin No 4220 - Growing High Yielding Wheat Crops In The Central Wheatbelt, Michael Perry

Bulletins 4000 -

In this Bulletin we hope to provide you with what we believe is the best and most up-to-date information about growing wheat in your particular environment.

It is now more important than ever for producers to get the most out of their resources of land, labour and capital. The key to doing that is the knowledge to do even better what you probably do well already-growing wheat.

The Australian Lupin Collection - Passport Data For Wild And Semi-Domesticated Accessions Introduced Into Australia To 1990, J. C. Clements, W. A. Cowling

Grain and other field crops published reports

This report contains passport (including collection site) data and Commonwealth Plant Introduction (CPI) numbers for all wild and semi-domesticated Lupinus accessions introduced into Australia to 1990 (a total of 1071 accessions with CPI numbers between 10650 and 122692). Scientists or plant breeders with an interest in the characteristics of wild lupins from certain natural environments, such as high soil pH or low rainfall, will find this information invaluable when they select accessions for their experiments. The majority of the accessions were introduced by Western Australian scientists following collecting missions in the Mediterranean region, and the remainder were donated from institutions …

Bulletin Ma 1/90 - Manjimup/Pemberton Vegetable Variety Planting Guide 1990, J. R. Burt, A G. Mckay, D. R. Phillips, M. A. Hegney

Horticulture published reports

AGDEX NO: 250/30

Manjimup Regional Office

Manjimup and Pemberton vegetable planting guide 1990.

Can Genetically Engineered Crops Become Weeds?, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

There are significant differences if the distribution of weedy characteristics among weeds, normal plants, and crops. The world’s most serious weeds possess on the average 10 or 11 of these characters, a random collection of British plants have an average seven of the traits, and crop plants only five. For the average crop to become as “weedy” as the average weed, it would need to acquire five weedy traits. Even using the unlikely assumption that those traits are single loci in which a dominant mutation would provide the weedy character, this would require the simultaneous acquisition of five gene substitutions. …

Introducing Onslow, Western Australian Department Of Agriculture

Grain and other field crops published reports

Onslow Barley........ a new breed of barley for the southern high rainfall areas (see map VH,H4 &H5). Suited to May plantings, Onslow is the first barley developed specifically for the long growing seasons in these areas.

For the first time barley growers have a variety designed for those 4 t/ha plus yields which research shows are possible with early sowing.

Onslow is the first of a new breed of barley cultivars developed in Western Australia specifically for the southern high rainfall areas which have a long growing season.

Looking At Lupins - Lupin Management In The South Eastern Wheatbelt, W. L. Smart, M. M. Ralph, J. M. Liddle, R.. D. Ramm, C. J. Robinson, E. W. Armstrong

Grain and other field crops research reports

In the past couple of years research into lupins has increased substantially particularly in the fields of weed control, seeding rates, and control of diseases such as brown leaf spot and pleiochaeta root rot. This report is a summary of the lupin trials in the Lake Grace advisory area as well as a guide to better lupin farming.

Grain Crop Variety Trials Western Australia 1978-1979, H. M. Fisher, G. B. Crosbie

Grain and other field crops research reports

The regional crop variety testing programme conducted by the Western Australian Department of Agriculture covers the final two stages of a four stage process of evaluating locally produced crossbreds and introduced material comprising:-

Plant Breeder Trials

Stage 1: Initial small plot yield tests on early generation material - 1 to 3 sites. Stage 2: Small plot yield tests on selected material (F5 derived) - 3 to 4 sites.

Regional Testing Programme

Stage 3: Large scale preliminary trials - 9 to 10 sites. Stage 4: Large scale final yield trials - 40 to 50 sites.

The basis of the regional programme …