Agricultural Science Commons^™

Comparison Between In Vitro And In Vivo Antibacterial Activity Of Curcuma Zedoaria From Malaysia., Behrooz Banisalam

Student Works (2010-2019)

The rhizomes of the Zingiberaceae family are vegetables widely used in many Asian countries and their medicinal functions have been broadly discussed and accepted in many traditional recipes. In this study, the antimicrobial activity of extracts of Curcuma zedoaria from Malaysia was compared between two systems as in vitro and in vivo. The comparison was performed against four bacterial strains including two gram negative strains (E.coli and Pseudomonas aeruginosa) and two gram positive strains (Bacillus cereus and Staphylococcus aureus) using the agar well diffusion method. Extracts were made in three different solvents; petroleum ether, chloroform and methanol extracts exhibited antibacterial …

Structural And Functional Divergence Of A 1-Mb Duplicated Region In The Soybean (Glycine Max) Genome And Comparison To An Orthologous Region From Phaseolus Vulgaris, Jer-Young Lin, Robert M. Stupar, Christian Hans, D. L. Hyten, Scott A. Jackson

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max) has undergone at least two rounds of polyploidization, resulting in a paleopolyploid genome that is a mosaic of homoeologous regions. To determine the structural and functional impact of these duplications, we sequenced two ~1-Mb homoeologous regions of soybean, Gm8 and Gm15, derived from the most recent ~13 million year duplication event and the orthologous region from common bean (Phaseolus vulgaris), Pv5. We observed inversions leading to major structural variation and a bias between the two chromosome segments as Gm15 experienced more gene movement (gene retention rate of 81% in Gm15 versus 91% in …

Effects Of Plant Growth Regulators And Physiological Stresses On The Growth Of Bougainvillea Spp., Mohammed Saifuddin

Student Works (2010-2019)

This study was aimed to investigate the effects of physiological stresses such as pruning, shading, phloemic stress and plant hormones such as gibberellic acid (GA3) and naphthalene acetic acid (NAA) on the growth, development and flowering process of bougainvillea plants. The plants (Bougainvillea glabra and Bougainvillea spectabilis) were grown under prevailing conditions (temperature 21-32°C, maximum PAR 2100 μE m-2 s-1 and relative humidity of 60-90%) using completely randomized design. Field experiments as well as biochemical analysis were carried out at the Plant Physiology Garden and the Plant Physiology Laboratory, Institute of Biological Sciences, University of Malaya. Results on frequent pruning …

Selecting Strawberry Cultivars For Winter Greenhouse Production, Ellen T. Paparozzi, Stacy A. Adams, George E. Meyer, M. Elizabeth Conley, Vicki L. Schlegel, Erin E. Blankenship, Paul E. Read

Department of Agronomy and Horticulture: Faculty Publications

Costs of transportation and food safety concerns have spawned an increase in public support of locally grown vegetables and fruit. With this in mind, a two-phase investigation was planned with the goal of combining low start up costs for sustainable greenhouse production with selection of strawberry cultivars that would provide the greatest number and largest size of berries. Additionally, berries from each cultivar will be analyzed for their beneficial nutraceutical properties to determine if there is a difference among cultivars.

Fine Mapping The Soybean Aphid Resistance Gene Rag1 In Soybean, Ki-Seung Kim, Stephanie Bellendir, Karen A. Hudson, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

The soybean aphid (Aphis glycines Matsumura) is an important soybean [Glycine max (L.) Merr.] pest in North America. The dominant aphid resistance gene Rag1 was previously mapped from the cultivar ‘Dowling’ to a 12 cM marker interval on soybean chromosome 7 (formerly linkage group M). The development of additional genetic markers mapping closer to Rag1 was needed to accurately position the gene to improve the effectiveness of markerassisted selection (MAS) and to eventually clone it. The objectives of this study were to identify single nucleotide polymorphisms (SNPs) near Rag1 and to position these SNPs relative to Rag1. …

A High Density Integrated Genetic Linkage Map Of Soybean And The Development Of A 1536 Universal Soy Linkage Panel For Quantitative Trait Locus Mapping, D. L. Hyten, Ik-Young Choi, Qijian Song, James E. Specht, Thomas E. Carter Jr., Randy C. Shoemaker, Eun-Young Hwang, Lakshmi K. Matukumalli, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Single nucleotide polymorphisms (SNPs) are the marker of choice for many researchers due to their abundance and the high-throughput methods available for their multiplex analysis. Only recently have SNP markers been available to researchers in soybean [Glycine max (L.) Merr.] with the release of the third version of the consensus genetic linkage map that added 1141 SNP markers to the map. Our objectives were to add 2500 additional SNP markers to the soybean integrated map and select a set of 1536 SNPs to create a universal linkage panel for high-throughput soybean quantitative trait locus (QTL) mapping. The GoldenGate assay …

Crop Registration: The Pathway To Public Access Of Plant Genetic Materials To Build Crops For The Future, D. D. Ellis, K. A. Garland-Campbell, J. A. Grotenhuis, M. M. Jenderek, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Starting in 1926 and continuing for 80+ yr, 11,241 crop genetic materials have been registered as of 31 Dec. 2008. The crop registration process is an important pathway to publically describe and document new and useful genetic materials and to incorporate these into the public domain via the National Plant Germplasm System (NPGS). Crop Registration materials are now searchable via the Germplasm Resources Information Network (GRIN) and demand for registered materials remains strong with more than 9150 registered accessions distributed in the past 26 yr by the NPGS. Guidelines continue to evolve to accommodate global factors effecting germplasm exchange such …

Efficacy Of Singular And Stacked Brown Midrib 6 And 12 In The Modification Of Lignocellulose And Grain Chemistry, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

In sorghum, brown midrib (bmr) 6 and 12 impair the last two steps of monolignol synthesis. bmr genes were introduced into grain sorghum to improve the digestibility of lignocellulosic tissues for grazing or bioenergy uses following grain harvest. Near-isogenic grain sorghum hybrids (AWheatland X RTx430) were developed containing bmr6, bmr12, and the bmr6 bmr12 double mutant (stacked), and their impacts were assessed in a two-year field study. The bmr genes did not significantly impact grain or lignocellulosic tissue yield. Lignocellulosic tissue from bmr6, bmr12, and stacked hybrids had reduced lignin content and increased in …

Heterosis In Sweet Sorghum And Selection Of A New Sweet Sorghum Hybrid For Use In Syrup Production In Appalachia, T. W. Pfeiffer, M. J. Bitzer, J. J. Toy, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Although heterosis is well established in grain and forage sorghum [Sorghum bicolor (L.) Moench], reports of heterosis in sweet sorghum are limited to results from grain sorghum × sweet sorghum hybrids. Recent development of cytoplasmic male-sterile sweet sorghum lines allows creation of sweet sorghum hybrids for research and industry. Male sterility may also affect allocation of photosynthate to plant parts, creating the potential to increase sugar content in stems by eliminating seed as a sink. The objectives of this study were to compare performance of A₃ cytoplasmic male-sterile lines and A₃ cytoplasmic male-sterile hybrids to fertile B …

Influences Of Wind And Sandblasting On The Endangered Blowout Penstemon, James Stubbendieck, Cheryl D. Dunn, Heidi L. Hillhouse, L. M. Landholt

Department of Agronomy and Horticulture: Faculty Publications

Blowout penstemon Penstemon haydenii is a federally endangered species growing only in areas with active wind erosion in sand dunes of the central United States. This early seral species declines as the blowout habitat stabilizes, allowing later seral species to increase. Blowout penstemon populations and plant size declined in the 1990s when precipitation was higher than normal, resulting in reduced sand movement. We conducted a greenhouse experiment to determine whether blowing sand influenced vigor and persistence of blowout penstemon seedlings. Treatments were wind, sandblasting, wind with sandblasting, and a control. The wind treatment was a constant 14 km h^{–1 …}

Fine Mapping Of The Scn Resistance Locus Rhg1-B From Pi 88788, Myungsik Kim, David L. Hyten, Andrew F. Bent, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is the most economically damaging soybean [Glycine max (L.) Merr.] pest in the USA and genetic resistance is a key component for its control. Although SCN resistance is quantitative, the rhg1 locus on chromosome 18 (formerly known as Linkage Group G) confers a high level of resistance. The objective of this study was to fi ne-map the rhg1-b allele that is derived from plant introduction (PI) 88788. F₂ and F₃ plants and F_3:4 lines from crosses between SCN resistant and susceptible genotypes were tested with genetic markers to …

High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of …

Inoculation With Arbuscular Mycorrhizal Fungi Or Crop Rotation With Mycorrhizal Plants Improves The Growth Of Maize In Limed Acid Sulfate Soil, Hasao Higo, Katsunori Isobe, Dong-Jin Kang, Kazuhiro Ujie, Rhae A. Drijber, Ryuichi Ishii

Department of Agronomy and Horticulture: Faculty Publications

Arbuscular mycorrhizal fungi (AMF) improve the uptake of immobile mineral nutrients such as phosphate, thereby improving plant growth. In acid sulfate soil (ASS), AMF spore density is generally low which impacts root colonization and phosphate uptake. Thus, inoculation may help increase AMF colonization of crops grown in ASS. AMF spore density decreases after cultivation of a non-host crop or bare fallow. In addition, preceding crops affect the growth and yield of subsequent crops. The production of AMF inocula requires AMF-compatible plants. The objective of the present study is to elucidate the effect of preceding crops on the persistence of inoculated …

Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping …

Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map-based cloning and for marker-assisted selection in plant breeding. The objectives of this study were to determine the abundance of SSRs in the soybean genome and to develop and test soybean SSR markers to create a database of locus-specific markers with a high likelihood of polymorphism. A total of 210,990 SSRs with di-, tri-, and tetranucleotide repeats of five or more were identified in the soybean whole genome sequence (WGS) which included 61,458 SSRs consisting of repeat units of di- (≥10), tri- (≥8), and tetranucleotide (≥7). Among …

Molecular Studies Of 5-Azacytidine-Induced Early-Flowering Lines Of Flax, Megan A. House

Theses and Dissertations (Comprehensive)

Several early-flowering flax (Linum usitatissimum L.) lines were derived from treatment of germinating seeds with 5-azacytidine in 1990. These lines are also shorter, have fewer leaves, and their DNA is hypomethylated, relative to their corresponding controls. The work presented in this thesis used early-flowering and control lines of the Royal (R) flax genotype, and the Large (L) flax genotroph. Firstly, levels of cytosine methylation were measured over a 24-hour period in the early-flowering line RE2 and its control (RC), using an HPLC method. Secondly, to determine the response of the flax lines to short-day conditions, control and early-flowering …

Analysis Of The Accase Mutation Profile Of Italian Ryegrass (Lolium Perenne Ssp. Multiflorum) Accessions Resistant To Accase Inhibitors, Thomas Stark

Inquiry: The University of Arkansas Undergraduate Research Journal

Lolium perenne ssp. multiflorum (Italian ryegrass) resistant to ACCase inhibiting herbicides has been reported in many wheat producing counties across Arkansas. Resistance is believed to be the result of point mutations creating amino acid substitutions in the CT domain of the plastidic ACCase gene. This study explores the occurrence of mutations in the ACCase gene of ryegrass populations. Plant material was collected and DNA was extracted from 10 Arkansas ryegrass populations. Six of the populations were known to be resistant to the ACCase inhibitor diclofop-methyl, while the remaining four populations were known to be susceptible to diclofop-methyl. Two highly conserved …

Effects Of Stratification, Warm Treatment, And Mechanical And Acid Scarification On The Emergence Of Yellow-Flowered Alfalfa (Medicago Sativa Subsp. Falcata) Seeds, Diane Narem

The Journal of Undergraduate Research

Yellow-flowered alfalfa (YFA) has great potential to improve rangelands in the Northern Plains. Greater than 99% of YFA seeds from soil were viable but less than 4% germinated under standard laboratory conditions. The objective was to determine if low germination was due to physical or physiological seed dormancy. The experiment utilized a random complete block design with two trials, eight replications per trial. Each replication consisted of ten treatments plus a control, four stratification treatments consisting of a combination of two temperatures (-5°C, -20°C) and two durations (48 hr, 72 hr), one warm treatment (4 min, 60°C), four sandpaper scarification …

Letter From The Dean, Lalit Verma

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Bulked Segregant Analysis Using The Goldengate Assay To Locate The Rpp3 Locus That Confers Resistance To Soybean Rust In Soybean, D. L. Hyten, James R. Smith, Reid D. Frederick, Mark L. Tucker, Qijian Song, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Few resistance loci to soybean rust (SBR), caused by Phakopsora pachyrhizi Syd., have been genetically mapped and linked to molecular markers that can be used for marker assisted selection. New technologies are available for single nucleotide polymorphism (SNP) genotyping that can be used to rapidly map traits controlled by single loci such as resistance to SBR. Our objective was to demonstrate that the highthroughput SNP genotyping method known as the GoldenGate assay can be used to perform bulked segregant analysis (BSA) to fi nd candidate regions to facilitate effi cient mapping of a dominant resistant locus to SBR designated Rpp3 …

Mapping And Confirmation Of A New Allele At Rpp1 From Soybean Pi 594538a Conferring Rb Lesion–Type Resistance To Soybean Rust, Nanda Chakraborty, Joe Curley, Reid D. Frederick, D. L. Hyten, Randall L. Nelson, Glen L. Hartman, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean rust, caused by Phakopsora pachyrhizi (H. Sydow & Sydow), is a destructive soybean [Glycine max (L.) Merr.] disease and identification of new resistance genes is essential for effective rust management. Our research objectives were to map and confirm the location of resistance gene(s) in PI 594538A using a population of 98 F_3:4 lines from across between PI 594538A (reddish-brown [RB] lesions) and the susceptible cultivar Loda (tan [TAN] lesions). The lines were inoculated with the P. pachyrhizi isolate ZM01-1 from Zimbabwe. The RB resistance in PI 594538A mapped on linkage group G as a single dominant gene. …

Agroecology As A Science, A Movement And A Practice: A Review, A. Wezel, S. Bellon, T. Doré, Charles A. Francis, D. Vallod, C. David

Department of Agronomy and Horticulture: Faculty Publications

Agroecology involves various approaches to solve actual challenges of agricultural production. Though agroecology initially dealt primarily with crop production and protection aspects, in recent decades new dimensions such as environmental, social, economic, ethical and development issues are becoming relevant. Today, the term ‘agroecology’ means either a scientific discipline, agricultural practice, or political or social movement. Here we study the different meanings of agroecology. For that we analyse the historical development of agroecology. We present examples from USA, Brazil, Germany, and France. We study and discuss the evolution of different meanings agroecology. The use of the term agroecology can be traced …

Detection And Validation Of Single Feature Polymorphisms Using Rna Expression Data From A Rice Genome Array, Sung-Hyun Kim, Prasanna R. Bhat, Xinping Cui, Harkamal Walia, Jin Xu, Steve Wanamaker, Abdelbagi M. Ismail, Clyde Wilson, Timothy J. Close

Department of Agronomy and Horticulture: Faculty Publications

Background: A large number of genetic variations have been identified in rice. Such variations must in many cases control phenotypic differences in abiotic stress tolerance and other traits. A single feature polymorphism (SFP) is an oligonucleotide array-based polymorphism which can be used for identification of SNPs or insertion/deletions (INDELs) for high throughput genotyping and high density mapping. Here we applied SFP markers to a lingering question about the source of salt tolerance in a particular rice recombinant inbred line (RIL) derived from a salt tolerant and salt sensitive parent. Results: Expression data obtained by hybridizing RNA to an oligonucleotide array …

Antibiotic Use In Animal Production: Environmental Concerns, Teshome H. Regassa, Richard K. Koelsch, Charles S. Wortmann, Richard F. Randle, Akwasi A. Abunyewa

Department of Agronomy and Horticulture: Faculty Publications

Introduction

Antibiotic1 use in animal production has led to improved feed use efficiency and increased growth rates. In turn, these resulted in reduced food production costs and reduced excrement of manure nutrients which may cause pollution problems.

On the other hand, antibiotic use leads to antibiotic resistance and a possible reduction in effectiveness of treatment options for both animals and humans. This publication addresses these issues.

Disease-causing microorganisms2, including bacteria,are the most diverse and numerous organisms on earth. Diverse in their habitat, environmental adaptation, and mechanisms of reproduction, they have short generations with high multiplication rates. Such characteristics help develop …

Plan To Support Grains Industry Development 2009-2012, Department Of Agriculture And Food, Western Australia

Grain and other field crops published reports

The Plan to Support Grains Industry Development (‘the Plan’) recognises that Western Australian agricultural industries are integral to the economic wellbeing of regional and rural communities. The Plan promotes a progressive and profitable grains industry through modern best practice services and research to enhance continuous improvement in farming and supply chain practices.

The Plan has been developed to align with the Government of Western Australia’s initiatives, strategies and tactics for ‘making every hectare count’ (see Appendix 1) and with grains industry needs as identified by industry stakeholders during a series of consultation forums. These industry needs will be addressed in …

A High-Oleic-Acid And Low-Palmitic-Acid Soybean: Agronomic Performance And Evaluation As A Feedstock For Biodiesel, George L. Graef, Bradley J. Lavallee, Patrick Tenopir, Mustafa Tat, Bruce Schweiger, Anthony J. Kinney, Jon H. Van Gerpen, Thomas E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Phenotypic characterization of soybean event 335-13, which possesses oil with an increased oleic acid content (> 85%) and reduced palmitic acid content (< 5%), was conducted across multiple environments during 2004 and 2005. Under these conditions, the stability of the novel fatty acid profile of the oil was not influenced by environment. Importantly, the novel soybean event 335-13 was not compromised in yield in both irrigated and non-irrigated production schemes. Moreover, seed characteristics, including total oil and protein, as well as amino acid profile, were not altered as a result of the large shift in the fatty acid profile. The novel oil trait was inherited in a simple Mendelian fashion. The event 335-13 was also evaluated as a feedstock for biodiesel. Extruded oil from event 335-13 produced a biodiesel with improved cold flow and enhanced oxidative stability, two critical fuel parameters that can limit the utility of this renewable transportation fuel.

Adding Value To Swine Manure Through Accurate Prediction Of Organic Nitrogen Availability, Charles S. Wortman, Charles Shapiro, Aaron Nygren

Department of Agronomy and Horticulture: Faculty Publications

Animal manure contains organic and inorganic nitrogen. The inorganic N, which is mostly ammonium-N but nitrate-N can be significant in composted manure, is typically considered to be 100% plant available if there are not significant losses, especially due to volatilization of ammonia-N. The availability of organic N, which is mostly from undigested and partially digested plant material needs to be mineralized to become plant available, is less predictable. Field research was conducted in eastern Nebraska to improve the basis for estimating organic N availability from injected or incorporated swine manure and from surface applied composted and stockpiled FM. The factors …

Mullewa Research Annexe Spring Field Day Future Farming Systems For North-Eastern Districts 21 August 2008, Department Of Agriculture, Western Australia

Grain and other field crops research reports

Papers:

• Mullewa Research Annexe activities 2008

• Wheat for today and the future

• Consequences of strongly acidic and alkaline soils

• Break crop influence on low rainfall heavy soil

• Chemical fallow options for the north-east agricultural region

• Herbicide tolerance of new wheat varieties

• Lowrainfall oilseed crops for food and biofuel

• Utilising stock to reduce business risk in Binnu! A case study

Registration Of ‘Darrell’ Wheat, A. M. H. Ibrahim, S. D. Haley, P. S. Baenziger, Y. Jin, M. A. C. Langham, J. Rickertsen, S. Kalsbeck, R. Little, J. Ingemansen, O. K. Chung, B. W. Seabourn, G. H. Bai, Ming-Shun Chen, D. V. Mcvey

Department of Agronomy and Horticulture: Faculty Publications

‘Darrell’ (Reg. No. CV-1024, PI 644224) hard red winter wheat (Triticum aestivum L.) was developed by the South Dakota State University–Agricultural Experiment Station and released in 2006 to seed producers by the South Dakota State University–Agricultural Experiment Station and the University of Nebraska Agricultural Experiment Station. Darrell was selected from the cross 2076-W12-11/‘Karl 92’ (PI 564245)//NE89526. NE89526 is an experimental line from the University of Nebraska with the pedigree ‘Lancota’ (CI 17389) selection/’Siouxland’ (PI 483469)//TX792729. 2076-W12-11 is an unreleased experimental line that originated from a population with an unidentified pedigree. Darrell was selected as an F3:4 line in 1998 …

Australian Temperate Strawberry Breeding Program (Albany Western Australia) Screening Of Advance And New Selections For Albany Western Australia - 2006/2007, Shane Trainer, Dennis Phillips, Hal

Horticulture research reports

Fourteen intermediate day neutral selections from the Australian temperate breeding program were compared in a June trial planting on a commercial property in the Albany region. Intermediate selections were derived from crosses made between 1999 and 2002. Three advanced 'short day' selections were compared for yield, both as trial plots (10 plants) and as large plot demonstrations of up to 500 plants. Accompanying these selections, six named day neutral, three short day and four Queensland cultivars of different runner origins were compared. Camarosa, Selva and Diamante were included as commercial controls.

Day neutral selections 02-083-24 and 02-83-311 were potentially superior …