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Articles 10291 - 10320 of 10662

Full-Text Articles in Agricultural Science

Grassland Resources And Protections In The Yellow River Source Zone On The Qinghai-Tibet Plateau, Xilai Li, Gary J. Brierley, Youming Qiao, George L. W. Perry, Jay King Gao Jan 2015

Grassland Resources And Protections In The Yellow River Source Zone On The Qinghai-Tibet Plateau, Xilai Li, Gary J. Brierley, Youming Qiao, George L. W. Perry, Jay King Gao

IGC Proceedings (1977-2023)

This paper summarises resources and protections of the Yellow River Source Zone on the Qinghai-Tibet Plateau. A concerted effort has been made to address concerns for overgrazing on the alpine steppe and alpine meadow landscapes in the source zone of the Yellow River. An assessment of the impacts of overgrazing includes consideration of the role of small mammals (on the one hand they are considered as a critical ecosystem engineer, on the other they are perceived as a major threatening pest). Analyzed in this paper are management options in the restoration of degraded grasslands.


Importance Of Livestock Production From Grasslands For National And Local Food And Nutritional Security In Developing Countries, Iain A. Wright Jan 2015

Importance Of Livestock Production From Grasslands For National And Local Food And Nutritional Security In Developing Countries, Iain A. Wright

IGC Proceedings (1977-2023)

Grazing lands cover more than a quarter of the world’s land surface, often on land that is unsuitable for other forms of use. Despite the perception that productivity is inherently low, the contribution of grasslands in food security in developing countries is significant. However the challenges of spatial and temporal variability of primary productivity need to be managed and mobility of livestock is key to this. Appropriate land management and governance arrangements are essential for facilitating this mobility and for creating the circumstances in which technical options for reducing variability and risk in livestock keeping can be deployed and to …


Production, Persistence And Diversity Of Species In Temperate Grasslands, Meredith L. Mitchell, Brendan P. Christy Jan 2015

Production, Persistence And Diversity Of Species In Temperate Grasslands, Meredith L. Mitchell, Brendan P. Christy

IGC Proceedings (1977-2023)

Temperate grassland areas are considered to be semi-arid, with rainfall typically between 250 and 500 mm, much of it occurring in the late spring and early summer. Grazing plays an important role in all these grasslands with impacts on diversity and persistence. Against this assumption that seasonal productivity would be more uniform within a pasture that was diverse, the research suggested that species rich pastures were less stable, because this species richness was composed of non-perennial, volunteer and weed species. This paper explores the concept that systems diversity of temperate grassland does not always equate to production. In these systems …


Using Various Lactic Acid Bacteria Strains During Forage Conservation Towards Fermentation, Storage, Nutritive Value And Safety Improvement, Jonas Jatkauskas, Vilma Vrotniakiene Jan 2015

Using Various Lactic Acid Bacteria Strains During Forage Conservation Towards Fermentation, Storage, Nutritive Value And Safety Improvement, Jonas Jatkauskas, Vilma Vrotniakiene

IGC Proceedings (1977-2023)

Many research efforts have been devoted to find ways how to produce silages with maximum nutritive value, good fermentation and low mould and yeast counts. While there are different objectives in using silage additives, the main objectives are to improve fermentation and reduce dry matter loss, and to prevent secondary fermentation at feed out time. This paper presents our most significant and recent investigations on forage inoculation before ensiling that have a potential for beneficial application on grassland and ruminant nutrition.


Traditional Livestock Production And Growth Opportunities In India, S. M. Deb Jan 2015

Traditional Livestock Production And Growth Opportunities In India, S. M. Deb

IGC Proceedings (1977-2023)

Livestock and animal health development projects need to be based on an understanding of livestock production systems because different systems need different approaches particularly in view of degradation of environment combined with population pressure. In India, the livestock production system is primarily traditional, consisting of mixed-farming and pastoral systems, therefore, development of marginal and smallholder mixed farms must aim at intensification of the total production system. Resource-poor farming systems may aim at the improved management of the various livestock species in backyards. Pastoral systems must focus on effective management of grazing pressure. Community pasture management involves application of technologies, land …


Nutrient Movements Through Ruminant Livestock Production Systems, Adegbola T. Adesogan, Jose C. B. Dubeux, Lynn E. Sollenberger Jan 2015

Nutrient Movements Through Ruminant Livestock Production Systems, Adegbola T. Adesogan, Jose C. B. Dubeux, Lynn E. Sollenberger

IGC Proceedings (1977-2023)

Considerable attention has been paid to reducing nutrient emissions from ruminant livestock in the last few decades. This area will continue to attract considerable research in the future due to increasing farm sizes in some developed countries as well as the increasing demand for meat and dairy products, particularly in developing countries. This paper discusses the deposition and losses of carbon and nitrogen in soils and plants in grazed and harvested forage systems as well as utilization and losses of both nutrients by ruminants in both systems. The paper also outlines several soil, plant, and animal-focused strategies that can be …


Rainwater Harvesting And Its Impact On Farming Systems, J. S. Samra Jan 2015

Rainwater Harvesting And Its Impact On Farming Systems, J. S. Samra

IGC Proceedings (1977-2023)

Landless, small holders and other poors supplement their livelihood and adapt to climate change by grazing, stall feeding with crop residues and fodder cultivated under rainfed and irrigated conditions. Improving productivity by conserving rainfall, ground water recharging, harvesting and recycling of rainwater especially in degraded open access or shared land with community participation was quite successful over a wide range of precipitation and ecological situations. Various types of trenches, bunding, vegetative barriers, gully plugs, ponds, check dams, land slides and mine spoils stabilization etc. retained more soil moisture, seeds, vegetative propagules etc. in situ and prevented soil erosion. It regenerated …


Strengthening Livelihood Of Rural Farmer Populations Through Improved Grasslands, Sujatha Premaratne, S. C. Somasiri Jan 2015

Strengthening Livelihood Of Rural Farmer Populations Through Improved Grasslands, Sujatha Premaratne, S. C. Somasiri

IGC Proceedings (1977-2023)

This paper reviews the current background of the grassland resource with a purpose of livestock perspective to improve the livelihood of rural farmers. The use of grasslands for livestock rearing by rural farmers has been a long tradition. In climate and vegetation contrast, these grasslands are much more diverse. They are likely to play an economical role in increasing the milk production in many Asian countries. With an increase of human population, the traditional feeding, breeding and surviving habitats for livestock have been acutely restricted in many countries. Therefore, a continued effort is needed to maintain production for sustainable management …


Problems And Prospects Of Grassland Development: Policy Issues, S. Ray Smith Jan 2015

Problems And Prospects Of Grassland Development: Policy Issues, S. Ray Smith

IGC Proceedings (1977-2023)

There are a range of government and non-government policies that affect grassland development around the world. The objective of this paper is to provide examples of successful policies which have been instrumental in the preservation and maintenance of grasslands and to highlight policies which have resulted in negative consequences. This paper will not attempt to provide a complete overview of policies, but instead will provide examples from representative countries. Policies which affect grasslands can be classified into two broad categories: government policies that cover comprehensive landscape issues like the Grassland Household Contract System (GHCS) in China or the Federal Land …


Management Of Grazing Lands Through Educating Communities, D. Ariungerel, Graeme Hand, Annabel Walsh Jan 2015

Management Of Grazing Lands Through Educating Communities, D. Ariungerel, Graeme Hand, Annabel Walsh

IGC Proceedings (1977-2023)

The Mongolian steppe is one of the world’s largest grassland and it is an arid to semiarid land with a strong climate gradient. Biodiversity loss leading to desertification and land degradation is estimated to be impacting around 65% of the total area of Mongolia. The preliminary objective of this project was to trial the Positive Deviance Methodology to determine if this method assists herders to design behaviors to reverse this biodiversity loss. This project is a partnership between Mongolian Herders, Mongolian rangeland scientists and Australian based Stipa Native Grasses Association. The Positive Deviance Methodology has five basic steps based around …


Livestock And Local Development: Going To A New Humananimal Relationship, Jean François Tourrand, Laurent Dobremez, Benoit Dedieu, Guillaume Duteurtre, M. G. Piketty, P. Lescoat, B. Hubert Jan 2015

Livestock And Local Development: Going To A New Humananimal Relationship, Jean François Tourrand, Laurent Dobremez, Benoit Dedieu, Guillaume Duteurtre, M. G. Piketty, P. Lescoat, B. Hubert

IGC Proceedings (1977-2023)

Along the past ten years, the French National Agency for Research (ANR) has financed projects regarding livestock. Results of five projects were gathered to understand the long-term livestock trends. At the end of the 19th century, animal breeding was oriented towards the production of goods to meet the local, regional, national and global demand, according to the zone. The market gradually became the key-factor to norm both production and consumption. It is now integrating environmental norms and is starting to invest in the social domain. However, this economical vision of animal production does not take into account the other functions …


Genomic Approaches For Dissecting Complex Traits Related To Quality Production Of Range Grasses, Masumi Ebina Jan 2015

Genomic Approaches For Dissecting Complex Traits Related To Quality Production Of Range Grasses, Masumi Ebina

IGC Proceedings (1977-2023)

Tropical grasses have been widely used as warm-season forage grasses in the warm temperate zone since the early 20th century because of their high yields; they have also been used as perennial forages in their native tropical areas. Increasing demand for animal production sparked by economic development in tropical countries is requiring breeders to improve native forage grasses in these countries. Considerable efforts have been made to breed accessions with improved characteristics and to develop new cultivars. However, cross-breeding is not common, owing to a lack of genetic information and to complexities related to polyploidy, high sterility rates, outcrossing, and …


Emission Of Green House Gases From Grasslands And Their Mitigation, Prem N. Sharma, Shyam Khadka Jan 2015

Emission Of Green House Gases From Grasslands And Their Mitigation, Prem N. Sharma, Shyam Khadka

IGC Proceedings (1977-2023)

The concentrations of green house gases (GHG) in the atmosphere began in pre-industrial times and it continues to increase. This could result into an alarming increase in temperature of up to 5.4 oC by year 2100 due to a net global annual GHG emission of H 4.5-6.5 Gt C equivalent. About 18% of the world’s GHG are contributed by livestock and related activities on grasslands that are spread over almost 35 million Km2.These grasslands give livelihood to over a billion people most of who are poor. Twenty to 70% of the land surface area is degrading often …


High-Altitude Grassland Management And Improvement Of Pastoral Livelihoods In The Hindu Kush Himalayan Region, Eklabya Sharma Jan 2015

High-Altitude Grassland Management And Improvement Of Pastoral Livelihoods In The Hindu Kush Himalayan Region, Eklabya Sharma

IGC Proceedings (1977-2023)

The Hindu Kush Himalayan (HKH) region is the largest mountain systems in the world, spanning over 4.3 million km2 and covering 3500 km long fragile environment. Grasslands in the HKH region are the source of livelihoods for approximately 25 to 30 million pastoralists and agro-pastoralists. High altitude grasslands play a significant role in storage and regulation of water; storage of carbon in soil and peat lands; permafrost storage; and stabilization of climate, soil, and nutrients. They foster a rich biodiversity with endemic species of fauna and flora; provide clean air and open spaces for recreational purposes, and bolster a rich …


Identification Of Novel Qtl Governing Root Architectural Traits In An Interspecific Soybean Population, Lakshmi P. Manavalan, Silvas J. Prince, Theresa A. Musket, Julian Chaky, Rupesh Deshmukh, Tri D. Vuong, Li Song, Perry B. Cregan, James C. Nelson, J. Grover Shannon, James E. Specht, Henry T. Nguyen Jan 2015

Identification Of Novel Qtl Governing Root Architectural Traits In An Interspecific Soybean Population, Lakshmi P. Manavalan, Silvas J. Prince, Theresa A. Musket, Julian Chaky, Rupesh Deshmukh, Tri D. Vuong, Li Song, Perry B. Cregan, James C. Nelson, J. Grover Shannon, James E. Specht, Henry T. Nguyen

Department of Agronomy and Horticulture: Faculty Publications

Cultivated soybean (Glycine max L.) cv. Dunbar (PI 552538) and wild G. soja (PI 326582A) exhibited significant differences in root architecture and root-related traits. In this study, phenotypic variability for root traits among 251 BC2F5 backcross inbred lines (BILs) developed from the cross Dunbar/PI 326582A were identified. The root systems of the parents and BILs were evaluated in controlled environmental conditions using a cone system at seedling stage. The G. max parent Dunbar contributed phenotypically favorable alleles at a major quantitative trait locus on chromosome 8 (Satt315-I locus) that governed root traits (tap root length and …


Genetic And Molecular Characterization Of Submergence Response Identifies Subtol6 As A Major Submergence Tolerance Locus In Maize, Malachy T. Campbell, Christopher A. Proctor, Yongchao Dou, Aaron J. Schmitz, Piyaporn Phansak, Greg R. Kruger, Chi Zhang, Harkamal Walia Jan 2015

Genetic And Molecular Characterization Of Submergence Response Identifies Subtol6 As A Major Submergence Tolerance Locus In Maize, Malachy T. Campbell, Christopher A. Proctor, Yongchao Dou, Aaron J. Schmitz, Piyaporn Phansak, Greg R. Kruger, Chi Zhang, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Maize is highly sensitive to short term flooding and submergence. Early season flooding reduces germination, survival and growth rate of maize seedlings. We aimed to discover genetic variation for submergence tolerance in maize and elucidate the genetic basis of submergence tolerance through transcriptional profiling and linkage analysis of contrasting genotypes. A diverse set of maize nested association mapping (NAM) founder lines were screened, and two highly tolerant (Mo18Wand M162W) and sensitive (B97 and B73) genotypes were identified. Tolerant lines exhibited delayed senescence and lower oxidative stress levels compared to sensitive lines. Transcriptome analysis was performed on these inbreds to provide …


Allmaps: Robust Scaffold Ordering Based On Multiple Maps, Haibo Tang, Xingtan Zhang, Chenyong Miao, Jisen Zhang, Ray Ming, James C. Schnable, Patrick S. Schnable, Eric Lyons, Jianguo Lu Jan 2015

Allmaps: Robust Scaffold Ordering Based On Multiple Maps, Haibo Tang, Xingtan Zhang, Chenyong Miao, Jisen Zhang, Ray Ming, James C. Schnable, Patrick S. Schnable, Eric Lyons, Jianguo Lu

Department of Agronomy and Horticulture: Faculty Publications

The ordering and orientation of genomic scaffolds to reconstruct chromosomes is an essential step during de novo genome assembly. Because this process utilizes various mapping techniques that each provides an independent line of evidence, a combination of multiple maps can improve the accuracy of the resulting chromosomal assemblies. We present ALLMAPS, a method capable of computing a scaffold ordering that maximizes colinearity across a collection of maps. ALLMAPS is robust against common mapping errors, and generates sequences that are maximally concordant with the input maps. ALLMAPS is a useful tool in building high-quality genome assemblies. ALLMAPS is available at: https://github.com/tanghaibao/jcvi/wiki/ALLMAPS.


Effects Of Sowing Date On Phenotypic Plasticity Of Fitness-Related Traits In Two Annual Weeds On The Songnen Plain Of China, Haiyan Li, John L. Lindquist, Yunfei Yang Jan 2015

Effects Of Sowing Date On Phenotypic Plasticity Of Fitness-Related Traits In Two Annual Weeds On The Songnen Plain Of China, Haiyan Li, John L. Lindquist, Yunfei Yang

Department of Agronomy and Horticulture: Faculty Publications

Background Phenotypic plasticity of fitness-related traits is vital for plant species to adapt to variable environments. Chenopodium glaucum L. and Amaranthus retroflexus L. are two common weed species globally. Understanding the plasticity in life-history traits, especially in reproductive allocation, within and among these species is important for predicting their success and for managing them in different environments.

Methodology/Principal Findings Seeds of the two plant species were sown every 10 days from 26 Jun to 15 Aug. Life-history and fitness-related traits of both phenology and morphology were measured, and dry biomass of roots, stems, leaves, and reproductive tissues was determined at …


Fingerprinting Soybean Germplasm And Its Utility In Genomic Research, Qijian Song, D. L. Hyten, Gaofeng Jia, Charles V. Quigley, Edward W. Fickus, Randall L. Nelson, P. B. Cregan Jan 2015

Fingerprinting Soybean Germplasm And Its Utility In Genomic Research, Qijian Song, D. L. Hyten, Gaofeng Jia, Charles V. Quigley, Edward W. Fickus, Randall L. Nelson, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

The United States Department of Agriculture, Soybean Germplasm Collection includes 18,480 domesticated soybean and 1,168 wild soybean accessions introduced from 84 countries or developed in the U.S. This collection was genotyped with the SoySNP50K BeadChip containing greater than 50K SNPs. Redundant accessions were identified in the collection and distinct genetic backgrounds of soybean from different geographic origins were observed that could be a unique resource for soybean genetic improvement. We detected a dramatic reduction of genetic diversity based on linkage disequilibrium and haplotype structure analyses of the wild, landrace and North American cultivar populations and identified candidate regions associated with …


Selective Genotyping For Marker Assisted Selection Strategies For Soybean Yield Improvement, Benjamin D. Fallen, Fred L. Allen, Dean A. Kopsell, Arnold M. Saxton, Leah Mchale, J. Grover Shannon, Stella K. Kantartzi, Andrea J. Cardinal, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone Jan 2015

Selective Genotyping For Marker Assisted Selection Strategies For Soybean Yield Improvement, Benjamin D. Fallen, Fred L. Allen, Dean A. Kopsell, Arnold M. Saxton, Leah Mchale, J. Grover Shannon, Stella K. Kantartzi, Andrea J. Cardinal, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Using molecular markers in soybean [Glycine max (L.) Merr.] has lead to the identification of major loci controlling quantitative and qualitative traits that include: disease resistance, insect resistance and tolerance to abiotic stresses. Yield has been considered as one of the most important quantitative traits in soybean breeding. Unfortunately, yield is a very complex trait and most yield quantitative trait loci (QTL) that have been identified have had only limited success for marker assisted selection (MAS). The objective of this study was to identify QTL associated with soybean seed yield in preliminary yield trials grown in different environments and …


Qtl For Seed Protein And Amino Acids In The Benning × Danbaekkong Soybean Population, C. V. Warrington, H. Abdel‑Haleem, D. L. Hyten, P. B. Cregan, J. H. Orf, A. S. Killam, N. Bajjalieh, Z. Li, H. R. Boerma Jan 2015

Qtl For Seed Protein And Amino Acids In The Benning × Danbaekkong Soybean Population, C. V. Warrington, H. Abdel‑Haleem, D. L. Hyten, P. B. Cregan, J. H. Orf, A. S. Killam, N. Bajjalieh, Z. Li, H. R. Boerma

Department of Agronomy and Horticulture: Faculty Publications

Soybean, rather than nitrogen-containing forages, is the primary source of quality protein in feed formulations for domestic swine, poultry, and dairy industries. As a sole dietary source of protein, soybean is deficient in the amino acids lysine (Lys), threonine (Thr), methionine (Met), and cysteine (Cys). Increasing these amino acids would benefit the feed industry. The objective of the present study was to identify quantitative trait loci (QTL) associated with crude protein (cp) and amino acids in the ‘Benning’ × ‘Danbaekkong’ population. The population was grown in five southern USA environments. Amino acid concentrations as a fraction of cp (Lys/cp, Thr/cp, …


Using A Simple Leaf Color Chart To Estimate Leaf And Canopy Chlorophyll A Content In Maize (Zea Mays), Anthony Nguy-Robertson, Yi Peng, Timothy Arkebauer, David Scoby, James Schepers, Anatoly Gitelson Jan 2015

Using A Simple Leaf Color Chart To Estimate Leaf And Canopy Chlorophyll A Content In Maize (Zea Mays), Anthony Nguy-Robertson, Yi Peng, Timothy Arkebauer, David Scoby, James Schepers, Anatoly Gitelson

Department of Agronomy and Horticulture: Faculty Publications

This study utilized a leaf color chart (LCC) to characterize the variation in leaf chlorophyll and estimate canopy chlorophyll in maize (Zea mays). The LCC consisted of four levels of greenness and was used to sort maize leaves in 2011 for three fields near Mead, Nebraska, USA. Leaf chlorophyll content for each color chart class was determined using two leaf-level sensors. The variation within each LCC class was reasonable (CV < 56%). The darkest color class predominated and indicated adequate fertilization rates using a SPAD. Canopy chlorophyll content was estimated using destructively measured leaf area index (LAI) and the LCC. This approach was verified with a method utilizing canopy reflectance collected by both satellite imagery and a four-band radiometer. The error between the two methods was reasonable (RMSE = 0.55-0.88 g m-2; CV = 25.6-50.4%), indicating that both leaf and canopy chlorophyll can be estimated cheaply without a wet lab or field-based sensors.


First Approximations Of Prescribed Fire Risks Relative To Other Management Techniques Used On Private Lands, Dirac L. Twidwell, Carissa L. Wonkka, Michael T. Sindelar, John R. Weir Jan 2015

First Approximations Of Prescribed Fire Risks Relative To Other Management Techniques Used On Private Lands, Dirac L. Twidwell, Carissa L. Wonkka, Michael T. Sindelar, John R. Weir

Department of Agronomy and Horticulture: Faculty Publications

Fire is widely recognized as a critical ecological and evolutionary driver that needs to be at the forefront of land management actions if conservation targets are to be met. However, the prevailing view is that prescribed fire is riskier than other land management techniques. Perceived risks associated with the application of fire limits its use and reduces agency support for prescribed burning in the private sector. As a result, considerably less cost-share support is given for prescribed fire compared to mechanical techniques. This study tests the general perception that fire is a riskier technique relative to other land management options. …


A Genomic Selection Index Applied To Simulated And Real Data, J. Jesus Ceron-Rojas, Jose Crossa, Vivi N. Arief, Kaye Basford, Jessica Rutkoski, Diego Jarquin, Gregorio Alvarado, Yoseph Beyene, Kassa Semagn, Ian Delacy Jan 2015

A Genomic Selection Index Applied To Simulated And Real Data, J. Jesus Ceron-Rojas, Jose Crossa, Vivi N. Arief, Kaye Basford, Jessica Rutkoski, Diego Jarquin, Gregorio Alvarado, Yoseph Beyene, Kassa Semagn, Ian Delacy

Department of Agronomy and Horticulture: Faculty Publications

A genomic selection index (GSI) is a linear combination of genomic estimated breeding values that uses genomic markers to predict the net genetic merit and select parents from a nonphenotyped testing population. Some authors have proposed a GSI; however, they have not used simulated or real data to validate the GSI theory and have not explained how to estimate the GSI selection response and the GSI expected genetic gain per selection cycle for the unobserved traits after the first selection cycle to obtain information about the genetic gains in each subsequent selection cycle. In this paper, we develop the theory …


Understanding Rice Adaptation To Varying Agro-Ecosystems: Trait Interactions And Quantitative Trait Loci, Shalabh Dixit, Alexandre Grondin, Cheng-Ruei Lee, Amelia Henry, Thomas-Mitchell Olds, Arvind Kumar Jan 2015

Understanding Rice Adaptation To Varying Agro-Ecosystems: Trait Interactions And Quantitative Trait Loci, Shalabh Dixit, Alexandre Grondin, Cheng-Ruei Lee, Amelia Henry, Thomas-Mitchell Olds, Arvind Kumar

Department of Agronomy and Horticulture: Faculty Publications

Background: Interaction and genetic control for traits influencing the adaptation of the rice crop to varying environments was studied in a mapping population derived from parents (Moroberekan and Swarna) contrasting for drought tolerance, yield potential, lodging resistance, and adaptation to dry direct seeding. A BC2F3-derived mapping population for traits related to these four trait groups was phenotyped to understand the interactions among traits and to map and align QTLs using composite interval mapping (CIM). The study also aimed to identify QTLs for the four trait groups as composite traits using multivariate least square interval mapping (MLSIM) …


Overexpression Of Sbmyb60 Impacts Phenylpropanoid Biosynthesis And Alters Secondary Cell Wall Composition In Sorghum Bicolor, Erin D. Scully, Tammy Gries, Gautam Sarath, Nathan A. Palmer, Lisa Baird, Michelle J. Serapiglia, Bruce S. Dien, Akwasi A. Boateng, Zhengxiang Ge, Deanna L. Funnell-Harris, Paul Twigg, Thomas E. Clemente, Scott E. Sattler Jan 2015

Overexpression Of Sbmyb60 Impacts Phenylpropanoid Biosynthesis And Alters Secondary Cell Wall Composition In Sorghum Bicolor, Erin D. Scully, Tammy Gries, Gautam Sarath, Nathan A. Palmer, Lisa Baird, Michelle J. Serapiglia, Bruce S. Dien, Akwasi A. Boateng, Zhengxiang Ge, Deanna L. Funnell-Harris, Paul Twigg, Thomas E. Clemente, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

The phenylpropanoid biosynthetic pathway that generates lignin subunits represents a significant target for altering the abundance and composition of lignin. The global regulators of phenylpropanoid metabolism may include MYB transcription factors, whose expression levels have been correlated with changes in secondary cell wall composition and the levels of several other aromatic compounds, including anthocyanins and flavonoids. While transcription factors correlated with downregulation of the phenylpropanoid biosynthesis pathway have been identified in several grass species, few transcription factors linked to activation of this pathway have been identified in C4 grasses, some of which are being developed as dedicated bioenergy feedstocks. In …


Aquaporins Contribute To Aba-Triggered Stomatal Closure Through Ost1-Mediated Phosphorylation, Alexandre Grondin, Olivier Rodrigues, Lionel Verdoucq, Sylvain Merlot, Nathalie Leonhardt, Christophe Maurel Jan 2015

Aquaporins Contribute To Aba-Triggered Stomatal Closure Through Ost1-Mediated Phosphorylation, Alexandre Grondin, Olivier Rodrigues, Lionel Verdoucq, Sylvain Merlot, Nathalie Leonhardt, Christophe Maurel

Department of Agronomy and Horticulture: Faculty Publications

Stomatal movements in response to environmental stimuli critically control the plant water status. Although these movements are governed by osmotically driven changes in guard cell volume, the role of membrane water channels (aquaporins) has remained hypothetical. Assays in epidermal peels showed that knockout Arabidopsis thaliana plants lacking the Plasma membrane Intrinsic Protein 2;1 (PIP2;1) aquaporin have a defect in stomatal closure, specifically in response to abscisic acid (ABA). ABA induced a 2-fold increase in osmotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive oxygen species in guard cells, which were both abrogated in …


A Dedicated Type Ii Nadph Dehydrogenase Performs The Penultimate Step In The Biosynthesis Of Vitamin K1 In Synechocystis And Arabidopsis, Abdelhak Fatihi, Scott Latimer, Stefan Schmollinger, Anna Block, Patrick Dussault, Wim F.J. Vermaas, Sabeeha S. Merchant, Gilles J. Basset Jan 2015

A Dedicated Type Ii Nadph Dehydrogenase Performs The Penultimate Step In The Biosynthesis Of Vitamin K1 In Synechocystis And Arabidopsis, Abdelhak Fatihi, Scott Latimer, Stefan Schmollinger, Anna Block, Patrick Dussault, Wim F.J. Vermaas, Sabeeha S. Merchant, Gilles J. Basset

Department of Agronomy and Horticulture: Faculty Publications

Mutation of Arabidopsis thaliana NAD(P)H DEHYDROGENASE C1 (NDC1; At5g08740) results in the accumulation of demethylphylloquinone, a late biosynthetic intermediate of vitamin K1. Gene coexpression and phylogenomics analyses showed that conserved functional associations occur between vitamin K biosynthesis and NDC1 homologs throughout the prokaryotic and eukaryotic lineages. Deletion of Synechocystis ndbB, which encodes for one such homolog, resulted in the same defects as those observed in the cyanobacterial demethylnaphthoquinone methyltransferase knockout. Chemical modeling and assay of purified demethylnaphthoquinone methyltransferase demonstrated that, by virtue of the strong electrophilic nature of S-adenosyl-Lmethionine, the transmethylation of the demethylated precursor …


Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding Jan 2015

Proteomic Profiling Of Maize Opaque Endosperm Mutants Reveals Selective Accumulation Of Lysine-Enriched Proteins, Kyla J. Morton, Shangang Jia, Chi Zhang, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Reduced prolamin (zein) accumulation and defective endoplasmic reticulum (ER) body formation occurs in maize opaque endosperm mutants opaque2 (o2), floury2 (fl2), defective endosperm*B30 (DeB30), and Mucronate (Mc), whereas other opaque mutants such as opaque1 (o1) and floury1 (fl1) are normal in these regards. This suggests that other factors contribute to kernel texture. A liquid chromatography approach coupled with tandem mass spectrometry (LC-MS/MS) proteomics was used to compare non-zein proteins of nearly isogenic opaque endosperm mutants. In total, 2762 proteins were identified that were enriched for biological processes such as protein transport and folding, amino acid biosynthesis, …


Tonoplast Sugar Transporters (Sbtsts) Putatively Control Sucrose Accumulation In Sweet Sorghum Stems, Saadia Bihmidine, Benjamin T. Julius, Ismail M. Dweikat, David M. Braun Jan 2015

Tonoplast Sugar Transporters (Sbtsts) Putatively Control Sucrose Accumulation In Sweet Sorghum Stems, Saadia Bihmidine, Benjamin T. Julius, Ismail M. Dweikat, David M. Braun

Department of Agronomy and Horticulture: Faculty Publications

Carbohydrates are differentially partitioned in sweet versus grain sorghums. While the latter preferentially accumulate starch in the grain, the former primarily store large amounts of sucrose in the stem. Previous work determined that neither sucrose metabolizing enzymes nor changes in Sucrose transporter (SUT) gene expression accounted for the carbohydrate partitioning differences. Recently, 2 additional classes of sucrose transport proteins, Tonoplast Sugar Transporters (TSTs) and SWEETs, were identified; thus, we examined whether their expression tracked sucrose accumulation in sweet sorghum stems. We determined 2 TSTs were differentially expressed in sweet vs. grain sorghum stems, likely underlying the massive difference in sucrose …