Agricultural Science Commons^™

Western Australia's Bulk And Containerised Grain Exports In A National Context, Ross Kingwell, Dipesh Maharjan

Research Reports

This report describes containerised and bulk grain exports from Western Australia and all other main grain-producing Australian states. The types of grain, their export volumes and destinations, since 2011-12 until 2021-22 are described.

Genome Architecture And Diverged Selection Shaping Pattern Of Genomic Differentiation In Wild Barley, Wenying Zhang, Cong Tan, Haifei Hu, Rui Pan, Yuhui Xiao, Kai Ouyang, Yong Jia, Xiao-Qi Zhang, Camilla Hill, Penghao Wang, Brett Chapman, Yong Han, Gaofeng Zhou, Le Xu, Yanhao Xu, Tefera Angessa, Hao Luo, Sharon Westcott, Darshan Sharma, Eviatar Nevo, Roberto Barrero, Matthew I. Bellgard, Tianhua He, Xiaohai Tian, Chengdao Li

Grain and Other Field Crops Research Articles

Divergent selection of populations in contrasting environments leads to functional genomic divergence. However, the genomic architecture underlying heterogeneous genomic differentiation remains poorly understood. Here, we de novo assembled two high-quality wild barley (Hordeum spontaneum K. Koch) genomes and examined genomic differentiation and gene expression patterns under abiotic stress in two populations. These two populations had a shared ancestry and originated in close geographic proximity but experienced different selective pressures due to their contrasting micro-environments. We identified structural variants that may have played significant roles in affecting genes potentially associated with well-differentiated phenotypes such as flowering time and drought response between …

Alfalfa Establishment And Management In South Carolina, Liliane Silva, Michael Mrshall, Jeremy Greene, Matias Aguerre

Livestock and Forages

Alfalfa is a perennial legume with high forage quality that can be used under grazing or hay management. This publication outlines establishment and management recommendations for alfalfa plantings in South Carolina.

Snp Discovery In Proso Millet (Panicum Miliaceum L.) Using Lowpass Genome Sequencing, Rituraj Khound, Guangchao Sun, Ravi V. Mural, James C. Schnable, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Domesticated ~10,000 years ago in northern China, Proso millet (Panicum miliaceum L.) is a climate-resilient and human health-promoting cereal crop. The genome size of this self-pollinated allotetraploid is 923 Mb. Proso millet seeds are an important part of the human diet in many countries. In the USA, its use is restricted to the birdseed and pet food market. Proso millet is witnessing gradual demand in the global human health and wellness food market owing to its health-promoting properties such as low glycemic index and gluten-free. The breeding efforts for developing improved proso millet cultivars are hindered by the …

Characterization And Selection Of Hop Cultivars Adapted To Nebraska, Kristina Alas

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Hop (Humulus lupulus L.) is an ingredient in the beer brewing industry that provides beer its flavor and aroma. High demand from the brewing industry has encouraged production outside of the traditional Pacific Northwest, the primary production region. Producers in the Midwest are attempting to grow cultivars adapted to the Pacific Northwest, but environmental differences have caused low yields and changes in secondary metabolite content. To aid producers, a regional breeding program was initiated to develop cultivars adapted to the Midwest. Success of any breeding program relies on the selection of genetically superior parents to generate progeny with the …

Muscadine Grapes: Identifying Unique Attributes And Postharvest Practices, John Cody Rawls

Graduate Theses and Dissertations

Arkansas has a long history of grape and wine production, and muscadine grapes (Vitis rotundifolia Michx.), a native disease-resistant grape, are an important part of that industry. Muscadine grapes can be sold as a fresh-market grape or made into juice, wine, and other products. Additionally, the University of Arkansas System Division of Agriculture (UA System) breeding program has a focus on creating new muscadine cultivars with commercial potential. The objectives of this research were to evaluate muscadine grape genotypes (cultivars and breeding selections) for fresh market consumption and wine production in 2020 and 2021. 33 seeded and seedless genotypes of …

The Genetic Basis For Panicle Trait Variation In Switchgrass (Panicum Virgatum), Li Zhang, Alice Macqueen, Xiaoyu Weng, Kathrine D. Behrman, Jason Bonnette, John L. Reilley, Francis M. Rouquette, Philip A. Fay, Yanqi Wu, Felix B. Fritschi, Robert B. Mitchell, David B. Lowry, Arvid R. Boe, Thomas E. Juenger

Department of Agronomy and Horticulture: Faculty Publications

Key message: We investigate the genetic basis of panicle architecture in switchgrass in two mapping populations across a latitudinal gradient, and find many stable, repeatable genetic effects and limited genetic interactions with the environment. Abstract: Grass species exhibit large diversity in panicle architecture influenced by genes, the environment, and their interaction. The genetic study of panicle architecture in perennial grasses is limited. In this study, we evaluate the genetic basis of panicle architecture including panicle length, primary branching number, and secondary branching number in an outcrossed switchgrass QTL population grown across ten field sites in the central USA through multi-environment …

Pestfacts Wa Issue 12 - August 2022, Alan Lord, Ciara Beard, Cindy L. Webster

PestFacts WA

PestFacts WA Issue 12 - August 2022 contents:

• Native budworm activity update
• Sclerotinia update
• Oat disease webinar recording can now be viewed online
• Explainer video for new PestFacts WA Reporter app

Pestfacts Wa Issue 13 - August 2022, Cindy L. Webster, Dusty Severtson, Alan Lord, Andrea Hills

PestFacts WA

PestFacts WA Issue 13 - August 2022 contents:

• Identifying caterpillars in cereal crops
• Diamondback moth update
• Native budworm
• Wheat powdery mildew fungicide resistance testing

Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan R. Mcpherson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Arbuscular mycorrhizal fungi (AMF) remain a vital obligate symbiont of nearly all plants. It is well established that the symbiosis between AMF and host plant improves plant nutrient acquisition, alleviates abiotic and biotic environmental stressors, defends against plant pathogens, and contributes to overall plant fitness and productivity through modification of the soil habitat. Modifications include increased soil aggregation and stability, carbon sequestration through provision of fungal wall precursors to soil organic matter (SOM) formation, and enhanced nutrient cycling in the mycorrhizosphere. The goal of this dissertation was to assess how AMF respond to nitrogen (N) fertilization regimes in maize cropping …

Quantification And Machine Learning Based N2o–N And Co2–C Emissions Predictions From A Decomposing Rye Cover Crop, Deepak R. Joshi, David E. Clay, Sharon A. Clay, Janet Moriles-Miller, Aaron L. M. Daigh, Graig Reicks, Shaina Westhoff

Department of Agronomy and Horticulture: Faculty Publications

Cover crops improve soil health and reduce the risk of soil erosion. However, their impact on the carbon dioxide equivalence (CO_2e) is unknown. Therefore, the objective of this 2-yr study was to quantify the effect of cover crop-induced differences in soil moisture, temperature, organic C, and microorganisms on CO_2e, and to develop machine learning algorithms that predict daily N₂O–N and CO₂–C emissions. The prediction models tested were multiple linear regression, partial least square regression, support vector machine, random forest (RF), and artificial neural network. Models’ performance was accessed using R², …

Registration Of Lcs ‘Valiant’ Hard Red Winter Wheat, P. Stephen Baenziger, S. D. Masterson, J. D. Boehm Jr., Vikas Belamkar, M. D. Barnett, Devin J. Rose, Lan Xu, Stephen N. Wegulo, T. Regassa, Amanda Easterly, Cody F Creech, Dipak K. Santra, G. R. Kruger, G. W. Hergert, R. N. Klein, J. Kolmer, J. Kolmer, M.-S. Chen, Gary L. Hein, R. L. Bowden, M. J. Guttieri, G. Bai, El-Basyoni Salah, J. Poland

Department of Agronomy and Horticulture: Faculty Publications

Historically cultivars developed jointly by the University of Nebraska-Lincoln and USDA-ARS wheat (Triticum aestivum L.) improvement project tend to be late and better adapted to the northern Great Plains. LCS ‘Valiant’ (Reg. no. CV-1196, PI 693223; tested as NE10478-1) was released based on the merits of its earliness, agronomic performance, relevant disease resistances, and end-use quality characteristics and its adaptation to the central Great Plains. As such, the line was licensed to Limagrain Cereal Seeds for their ability to market outside of Nebraska. LCS Valiant hard red winter wheat was released in March 2020 by the developing institutions and …

Association Analyses Of Host Genetics, Root-Colonizing Microbes, And Plant Phenotypes Under Different Nitrogen Conditions In Maize, Michael A. Meier, Gen Xu, Martha G. Lopez-Guerrero, Guangyong Li, Christine Smith, Brandi Sigmon, Joshua R. Herr, James R. Alfano, Yufeng Ge, James C. Schnable, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

The root-associated microbiome (rhizobiome) affects plant health, stress tolerance, and nutrient use efficiency. However, it remains unclear to what extent the composition of the rhizobiome is governed by intraspecific variation in host plant genetics in the field and the degree to which host plant selection can reshape the composition of the rhizobiome. Here, we quantify the rhizosphere microbial communities associated with a replicated diversity panel of 230 maize (Zea mays L.) genotypes grown in agronomically relevant conditions under high N (+N) and low N (-N) treatments. We analyze the maize rhizobiome in terms of 150 abundant and consistently reproducible …

Replacing Fallow With Field Pea In Wheat Production Systems Across Western Nebraska, Samuel T. Koeshall, Amanda Easterly, Rodrigo Werle, Strahinja Stepanovic, Cody F. Creech

Department of Agronomy and Horticulture: Faculty Publications

Integration of field pea (Pisum sativum L.) (FP) into dryland cropping systems has increased due to ecological and economic benefits, paired with a growing market for pea-derived products. Challenges exist in the High Plains that limit the integration of crop rotations to replace fallow periods with FP in wheat (Triticum aestivum L.)- based systems. This experiment compares chemical summer fallow to FP in a fallow– wheat rotation at two locations in western Nebraska. Soil water content, soil fertility, N mineralization, FP yield, and subsequent hard red winter wheat (HWW) yields were recorded. Subsequent HWW yields were not different …

Non-Destructive Classification And Quality Evaluation Of Proso Millet Cultivars Using Nir Hyperspectral Imaging With Machine Learning, Laruen E. Doyle, Julia R. Loeb, Nader Ekramirad, Dipak K. Santra, Akinbode A. Adedeji

Department of Agronomy and Horticulture: Faculty Publications

Millet is a small-seeded cereal crop with big potential and remarkable characteristics such as high drought resistance, short growing time, low water footprint, and the ability to grow in acidic soil. There is a need to develop nondestructive methods for differentiation and evaluation of the quality attributes of different of proso millet cultivars grown in the U.S. Current methods of cultivar classification are either subjective or destructive, time consuming, not allowing for the whole population to be tested, and requiring trained operators and special equipment. In this study, the feasibility of using near-infrared (NIR) hyperspectral imaging (900-1700 nm) to predict …

Pestfacts Wa Issue 09 - July 2022, Cindy L. Webster, Svetlana Micic, Dusty Severtson, Alan Lord

PestFacts WA

PestFacts WA Issue 09 - July 2022 contents:

• Diamondback moth

Helping Utah Landowners Reduce Pesticide Use Through A Statewide Ipm Program, Nick Volesky, Mair Murray

Outcomes and Impact Quarterly

The Utah Integrated Pest Management (IPM) program provides outreach and education to Utahns to reduce pesticide use and ultimately protect human and environmental health. In 2022, the IPM program responded to stakeholders’ needs by partnering with county Extension offices to deliver eight (8) in-person workshops across the state. The workshops connected Extension specialists and county faculty with managers of home landscapes and farms. The objective of the workshops was to provide participants with IPM skills to identify, monitor, and manage pest problems in preparation for the upcoming growing season.

Optimization Of Nozzle, Application Height, And Speed For Uass Pesticide Applications, Trenton Houston

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Unmanned aerial spray systems (UASS) applications have the potential to be efficient pesticide application platforms under conditions that are not accessible or fit for typical pesticide application equipment. Although this type of application is still under development in the United States, UASS pesticide applications are common in Asia, as they have replaced backpack sprayers. There is limited literature on the optimization of UASS applications and many parameters need to be investigated to identify the best combination of application variables such as flight height, flight speed, and nozzle selection. The objectives were to identify the deposition patterns of a four rotor …

Increased Signal-To-Noise Ratios Within Experimental Field Trials By Regressing Spatially Distributed Soil Properties As Principal Components, Jeffrey C. Berry, Mingsheng Qi, Balasaheb V. Sonawane, Amy Sheflin, Asaph Cousins, Jessica Prenni, Daniel P. Schachtman, Peng Liu, Rebecca S. Bart

Department of Agronomy and Horticulture: Faculty Publications

Environmental variability poses a major challenge to any field study. Researchers attempt to mitigate this challenge through replication. Thus, the ability to detect experimental signals is deter-mined by the degree of replication and the amount of environmental variation, noise, within the experimental system. A major source of noise in field studies comes from the natural heterogeneity of soil properties which create microtreatments throughout the field. In addition, the variation within different soil properties is often nonrandomly distributed across a field. We explore this challenge through a sorghum field trial dataset with accompanying plant, microbiome, and soil property data. Diverse sorghum …

Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang

Department of Agronomy and Horticulture: Faculty Publications

Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo’s responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and …

Neuropilin-2 Regulates Androgen-Receptor Transcriptional Activity In Advanced Prostate Cancer, Samikshan Dutta, Navatha Shree Polavaram, Ridwan Islam, Sreyashi Bhattacharya, Sanika Bodas, Thomas Mayr, Sohini Roy, Sophie Alvarez, Marieta I. Toma, Anza Darehshouri, Angelika Borkowetz, Stefanie Conrad, Susanne Fuessel, Manfred Wirth, Gustavo B. Baretton, Paramita Ghosh, Kenneth J. Pienta, David L. Klinkebiel, Surinder K. Batra, Michael H. Muders, Kaustubh Datta

Department of Agronomy and Horticulture: Faculty Publications

Aberrant transcriptional activity of androgen receptor (AR) is one of the dominant mechanisms for developing of castration-resistant prostate cancer (CRPC). Analyzing AR-transcriptional complex related to CRPC is therefore important towards understanding the mechanism of therapy-resistance. While studying its mechanism, we observed that a transmembrane protein called neuropilin-2 (NRP2) plays a contributory role in forming a novel AR-transcriptional complex containing nuclear pore proteins. Using immunogold electron microscopy, high-resolution confocal microscopy, chromatin immunoprecipitation, proteomics, and other biochemical techniques, we delineated the molecular mechanism of how a specific splice variant of NRP2 becomes sumoylated upon ligand stimulation and translocates to the inner nuclear …

Soybean Management For Seed Composition: The Perspective Of U.S. Farmers, Andre F. Borja Reis, Luiz Rosso, Dan Davidson, Peter Kovacs, Larry C. Purcell, Frederick E. Below, Shaun Casteel, Hans J. Kandel, Seth Naeve, Sotirios V. Archontoulis, Ignacio A. Ciampitti

Agronomy, Horticulture and Plant Science Faculty Publications

The soybean [Glycine max (L.) Merr.] compositional quality is mainly provided by the seed concentration of protein and oil. These traits are critical for sustaining global use, and although there is demand for high protein soybean, no mechanism to differentiate production is in place. At the opposite end of the supply chain, farmers are remunerated on a mass basis without having any incentive regarding seed composition. This study evaluated farmers' perspectives and knowledge on soybean quality and their propensity to adopt quality improvement technologies. Farmers from the main U.S. producing regions (n = 271) were investigated with a …

Pestfacts Wa Issue 08 - July 2022, Benjamin S. Congdon, Svetlana Micic, Cindy L. Webster, Jean Galloway, Janette Pratt

PestFacts WA

PestFacts WA Issue 08 - July 2022 contents:

• Cold winter conditions are reducing winged aphid activity
• Russian wheat aphid
• UCI BlacklegCM – A new decision support tool for upper canopy blackleg management

Pestfacts Wa Issue 10 - July 2022, Saleh Adnan, Benjamin S. Congdon, Alan Lord

PestFacts WA

PestFacts WA Issue 10 - July 2022 contents:

• Russian wheat aphids found at Geraldton
• Turnip yellows virus detected n canola in Albany port zone
• Meet PestFacts WA team member – Alan Lord

Pestfacts Wa Issue 11 - July 2022, Ciara Beard, Geoff J. Thomas

PestFacts WA

PestFacts WA Issue 11 - July 2022 contents:

• Wheat powdery mildew in the Geraldton port zone

Fine-Mapping And Characterisation Of Genes On Barley (Hordeum Vulgare) Chromosome 2h For Salinity Stress Tolerance During Germination, Edward Mwando, Yong Han, Tefera Angessa, Xiao-Qi Zhang, Chengdao Li

Grain and Other Field Crops Research Articles

Salinity causes a detrimental impact on plant growth, particularly when the stress occurs during germination and early development stages. Barley is one of the most salt-tolerant crops; previously we mapped two quantitative trait loci (QTL) for salinity tolerance during germination on the short arm of chromosome 2H using a CM72/Gairdner doubled haploid (DH) population. Here, we narrowed down the major QTL to a region of 0.341 or 0.439 Mb containing 9 or 24 candidate genes belonging to 6 or 20 functional gene families according to barley reference genomes v1 and v3 respectively, using two DH populations of CM72/Gairdner and Skiff/CM72, …

Identification Of Putative Snp Markers Associated With Resistance To Egyptian Loose Smut Race(S) In Spring Barley, Kamal A.M. Abo-Elyousr, Amira M.I. Mourad, P. Stephen Baenziger, Abdelaal H.A. Shehata, Peter E. Eckstein, Aaron D. Beattie, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Loose smut (LS) disease is a serious problem that affects barley yield. Breeding of resistant cultivars and identifying new genes controlling LS has received very little attention. Therefore, it is important to understand the genetic basis of LS control in order to genetically improve LS resistance. To address this challenge, a set of 57 highly diverse barley genotypes were inoculated with Egyptian loose smut race(s) and the infected seeds/plants were evaluated in two growing seasons. Loose smut resistance (%) was scored on each genotype. High genetic variation was found among all tested genotypes indicating considerable differences in LS resistance that …

Sorghum Association Panel Whole-Genome Sequencing Establishes Cornerstone Resource For Dissecting Genomic Diversity, J. Lucas Boatwright, Sirjan Sapkota, Hongyu Jin, James C. Schnable, Zachary Brenton, Richard Boyles, Stephen Kresovich

Department of Agronomy and Horticulture: Faculty Publications

Association mapping panels represent foundational resources for understanding the genetic basis of phenotypic diversity and serve to advance plant breeding by exploring genetic variation across diverse accessions. We report the whole-genome sequencing (WGS) of 400 sorghum (Sorghum bicolor (L.) Moench) accessions from the Sorghum Association Panel (SAP) at an average coverage of 38× (25–72×), enabling the development of a high-density genomic marker set of 43 983 694 variants including single-nucleotide polymorphisms (approximately 38 million), insertions/deletions (indels) (approximately 5 million), and copy number variants (CNVs) (approximately 170 000). We observe slightly more deletions among indels and a much higher prevalence …

Pestfacts Wa Issue 07 - June 2022, Alan Lord, Dusty Severtson, Cindy L. Webster, Svetlana Micic, Kithsiri Jayasena, Geoff J. Thomas, Ciara Beard, Andrea Hills, Jean Galloway

PestFacts WA

PestFacts WA Issue 07 - June 2022 contents:

• Native budworm moths and caterpillars are being found early
• 2022 winter spring insecticide spray guide is now available
• Monitor barley crops for net blotches
• Powdery mildew in barley and wheat
• Sclerotinia apothecia
• Spartacus leaf tipping

Recombination Hotspots In Soybean [Glycine Max (L.) Merr.], Samantha J. Mcconaughy

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Recombination allows for the exchange of genetic material between two parents which plant breeders exploit to make new and improved varieties. This recombination is not distributed evenly across the chromosome. In crops, it mostly occurs in the euchromatic regions of the genome and even then, recombination is focused into recombination hotspots flanked by recombination cold spots. Understanding the distribution of these hotspots along with the sequence motifs associated with them may lead to methods that enable breeders to better exploit recombination in breeding.

In chapter 1 background information on recombination, recombination hotspots detection methods, landscape of recombination (describe recombination patterns …