Agricultural Science Commons^™

Early Growth Stages Salinity Stress Tolerance In Cm72 X Gairdner Doubled Haploid Barley Population, Tefera T. Angessa, Xiao-Qi Zhang, Gaofeng Zhou, Sue Broughton, Wenying Zhang, Chengdao Li

Grain and Other Field Crops Research Articles

A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H–6H each …

Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff

Department of Agronomy and Horticulture: Faculty Publications

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.

Hanford Ffa Ornamental Horticulture Unit Master Plan, Savanah Diane Rhine, Russell Alan Zimmerman

Agricultural Education and Communication

This project is intended to develop a more functional plan for the Ornamental Horticulture (OH) Unit at the Hanford Agriculture Program. The ag program combines all three high schools in Hanford resulting in over 1,000 students; however, a majority of these students are not in the OH Career Pathway due to a lack of a constant, fully functional unit. As a comprehensive agriculture program, it is crucial that it incorporates all aspects of agriculture, not just animal science and agricultural mechanics.

This project will provide the Hanford agriculture program with a plan to be implemented so that they may have …

Planning For The Proposed Peel Food Zone, Ghd

Natural resources commissioned reports

Transform Peel is a $49m Royalties for Regions funded program. This program is broadly regarded as transformational for the Peel region in terms of job creation, sustainable growth and economic development. The Peel Development Commission and its partners, intend to implement this program over 35 years where projections estimate that 35 000 jobs will be created generating an approximate $16.2b per annum economic output by 2050. Located in the Shires of Murray and Serpentine Jarrahdale, the program comprises three integrated, strategic elements: Peel Food Zone; Peel Business Park; Peel Integrated Water Initiative.

The Department of Agriculture and Food, Western Australia …

Transcriptomic Resources For The Medicinal Legume Mucuna Pruriens: De Novo Transcriptome Assembly, Annotation, Identification And Validation Of Est-Ssr Markers, N. Sathyanarayana, Ranjith Kumar Pittala, Pankaj Kumar Tripathi, Ratan Chopra, Heikham Russiachand Singh, Vikas Belamkar, Pardeep Kumar Bhardwaj, Jeff J. Doyle, Ashley N. Egan

Department of Agronomy and Horticulture: Faculty Publications

Background:

The medicinal legume Mucuna pruriens (L.) DC. has attracted attention worldwide as a source of the anti-Parkinson’s drug L-Dopa. It is also a popular green manure cover crop that offers many agronomic benefits including high protein content, nitrogen fixation and soil nutrients. The plant currently lacks genomic resources and there is limited knowledge on gene expression, metabolic pathways, and genetics of secondary metabolite production. Here, we present transcriptomic resources for M. pruriens, including a de novo transcriptome assembly and annotation, as well as differential transcript expression analyses between root, leaf, and pod tissues. We also develop microsatellite markers …

A Comprehensive Analysis Of Alternative Splicing In Paleopolyploid Maize, Wenbin Mei, Sanzhen Liu, James C. Schnable, Cheng-Ting Yeh, Nathan M. Springer, Patrick S. Schnable, William Brad Barbazuk

Department of Agronomy and Horticulture: Faculty Publications

Identifying and characterizing alternative splicing (AS) enables our understanding of the biological role of transcript isoform diversity. This study describes the use of publicly available RNA-Seq data to identify and characterize the global diversity of AS isoforms in maize using the inbred lines B73 and Mo17, and a related species, sorghum. Identification and characterization of AS within maize tissues revealed that genes expressed in seed exhibit the largest differential AS relative to other tissues examined. Additionally, differences in AS between the two genotypes B73 and Mo17 are greatest within genes expressed in seed. We demonstrate that changes in the level …

The Effect Of Cattle Management On Soil Carbon: Implications For Climate Change, Kelly M. Livernoche

Masters Theses, 2010-2019

In naturally occurring ecosystems, forests function as substantial carbon sinks, storing carbon in soil and in biomass that would otherwise exist in the atmosphere as carbon dioxide. The conversion of forested land to cattle pastures and their associated operational processes are noteworthy contributors to recent increases in global carbon emissions and subsequent climate change. However, appropriately managed cattle pastures have potential to be reservoirs for carbon. Rotational cattle pastures, where cattle are moved between enclosed sections of pasture, may improve soil carbon content compared to conventional practices. In rotational cattle pastures, a more even distribution of manure increases plant biomass, …

Nutrient Loading Reduction In A Tile Drained Agricultural Watershed Through Watershed-Scale Cover Cropping: A High Resolution Analysis, Benjamin Gerald Bruening

Theses and Dissertations

Nutrient pollution originating from agricultural regions in the Midwest is a serious issue, leading to pollution of drinking water sources as well as large hypoxic zones in the Gulf of Mexico. The source of much of this contamination has been shown to be runoff from agricultural fields in the Upper Mississippi River Basin. One method that has been shown to reduce this pollution from the Upper Mississippi River Basin is the planting of winter cover crops. Winter cover crops such as rye and tillage radish have been shown to significantly reduce nitrate exported from agricultural fields, even in tile-drained watersheds …

Determining The Effects Of Sulfur Fertility Levels On Edamame Soybean [ Glycine Max ( L. ) Merrill ] Protein Components, Theoneste Nzaranyimana

Theses and Dissertations

Edamame [Glycine max (L.) Merr.] a vegetable type soybean is harvested and consumed before reaching its maturity stage (R6). Its health properties drew attention to U.S. consumers because of its high protein content. Proteins are the major structural component of all cells in the living organisms. They are made of essential and non-essential amino acids which play a big role in growth and development and are source of energy. Proteins act as enzymes, antibodies, and hormones and blood protein. Sulfur (S) is a component of the amino acids cysteine (Cys) and methionine (Met) which are the building blocks for proteins. …

Impact Of Fertilizer, Corn Residue, And Cover Crops On Mycorrhizal Inoculum Potential And Arbuscular Mycorrhizal Fungi Associations, Rachel Brockamp

Undergraduate Research Symposium 2017

Arbuscular Mycorrhizal Fungi (AMF) can associate with roots of most land plants, helping to take up water and nutrients. Intensive agricultural practices like fallow treatments and adding inorganic nutrients reduce soil AMF. The purpose of my research was to examine whether three factors influenced AMF-crop associations: 1) fertilizer type and application rate 2) inclusion of cover crops that do or do not associate with AMF, and 3) impacts of corn residue. Soil Mycorrhizal Inoculum Potential (MIP) was measured to test treatment differences. MIP is the ability for soil AMF to infect roots. Roots grown in soil from the different treatments …

Emergence, Competition, And Management Of Glyphosate-Resistant Common Ragweed (Ambrosia Artemisiifolia L.) In Nebraska Soybean, Ethann R. Barnes

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Common ragweed (Ambrosia artemisiifolia L.) is a competitive annual broadleaf weed in soybean (Glycine max) production fields throughout North America. The recent confirmation of glyphosate-resistant common ragweed in Nebraska justified the need to assess the emergence pattern and competitive ability of common ragweed in soybean and to evaluate alternative herbicide programs for effective management. The objectives of this research were to: 1) evaluate the effect of tillage and develop a predictive model for the emergence pattern of common ragweed in Nebraska; 2) model the competitive interaction between soybean and common ragweed as influenced by density and irrigation …

Investigation De Regeneración Natural De Plantas Vasculares En La Reserva Madrigal Del Podocarpus / Investigation Of Natural Regeneration Of Vascular Plants In The Madrigal Reserve Of The Podocarpus, Sy Baker

Independent Study Project (ISP) Collection

En el Sur de Ecuador, hay una historia larga de incendio que está asociada con la historia de la agricultura y ganadería a causa de las técnicas de tala y quema para despejar áreas para campos y también quemaduras intencionales para fertilizar los suelos y mejorar productividad agrícola (Fernandez et al 2015). En los últimos días de Noviembre de 2016, un incendio quemó campos, la Reserva Madrigal de Podocarpus, y parte del Parque Nacional Podocarpus en Loja, Ecuador—el enfoque de este estudio. En este estudio: (1) se registraron las especies y distribución de plantas pioneras y sobrevivientes, (2) la se …

Proceedings Of The 44th Annual Meeting, Southern Soybean Disease Workers (March 8-9, 2017, Pensacola Beach, Florida), Terry Spurlock, Trey Price, Heather M. Kelly, Danise Beadle, Patricia Bollich, Tom Allen

Southern Soybean Disease Workers: Conference Proceedings

Contents

Agenda for March 8-9, 2017

Contributed papers

Soybean cyst nematode: current status, challenges and opportunities. G Tylka and K Bissonnette

Updates of the 2nd SCN (Soybean Cyst Nematode) Coalition. Albert Tenuta

Developing management zones for nematodes in soybean. C Overstreet, EC McGawley, DM Xavier-Mis, and M Kularathna

Microbial communities associated with long-term tillage and fertilizer management practices. AY Srour, J Hackman, RL Cook, JP Bond, and AM Fakhoury

Microbial profile of SDS-suppressive soils in soybean fields. AY Srour, LFS Leonardo, DK Malvick, JP Bond, and AM Fakhoury

Understanding the phytobiome; using strip trials and spatial analysis to determine concomitant …

The Lx Report Of The Bean Improvement Cooperative No. 60, March 2017.

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

CONTENTS

LX Annual Report of the Bean Improvement Cooperative xi

BIC Coordinating Committee Membership - 1957 to 2017 . xii

BIC Meritorious Service and Distinguished Achievement Award Recipients - 1957 to 2015 xiii

BIC Awards Nomination Request for 2017 xvi

BIC/NAPIA Meeting in 2017 . xix

In Memory of Guillermo E. Galvez-Enriquez xx

RESEARCH PAPERS FOR 2017

OPTIMIZING SPORULATION OF Pseudocercospora griseola IN VITRO • Paula F. de Pádua1, Rafael Pereira1, Luanna B. W. Gomes1 and Elaine A. de Souza1* 1

FINE MAPPING THE BROAD SPECTRUM ANTHRACNOSE RESISTANCE GENE IN AMENDOIM CAVALO 3 • Gilio, T.A.S.1, Oscar P. Hurtado-Gonzales2, Giseli …

Towards The Development Of A Sustainable Soya Bean-Based Feedstock For Aquaculture, Hyunwoo Park, Steven Weier, Fareha Razvi, Pamela A. Peña, Neil A. Sims, Jennica Lowell, Cory Hungate, Karma Kissinger, Gavin Key, Paul Fraser, Jonathan Napier, Edgar B. Cahoon, Thomas Clemente

Center for Plant Science Innovation: Faculty Publications

Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean-based protein and a high Omega-3 fatty acid soya bean oil, enriched with alpha-linolenic …

Corn Residue Use By Livestock In The United States, Marty R. Schmer, Rachael M. Brown, Virginia L. Jin, Robert B. Mitchell, Daren D. Redfearn

Department of Agronomy and Horticulture: Faculty Publications

Corn (Zea mays L.) residue grazing or harvest provides a simple and economical practice to integrate crops and livestock, but limited information is available on how widespread corn residue utilization is practiced by US producers. In 2010, the USDA Economic Research Service surveyed producers from 19 states on corn grain and residue management practices. Total corn residue grazed or harvested was 4.87 million ha. Approximately 4.06 million ha was grazed by 11.7 million livestock (primarily cattle) in 2010. The majority of grazed corn residue occurred in Nebraska (1.91 million ha), Iowa (385,000 ha), South Dakota (361,000 ha), and Kansas …

A Comprehensive Image-Based Phenomic Analysis Reveals The Complex Genetic Architecture Of Shoot Growth Dynamics In Rice (Oryza Sativa), Malachy T. Campbell, Qian Du, Kan Liu, Chris J. Brien, Bettina Berger, Chi Zhang, Harkamal Walia

Agronomy & Horticulture -- Faculty Publications

Early vigor is an important trait for many rice (Oryza sativa L.)- growing environments. However, genetic characterization and improvement for early vigor is hindered by the temporal nature of the trait and strong genotype × environment effects. We explored the genetic architecture of shoot growth dynamics during the early and active tillering stages by applying a functional modeling and genomewide association (GWAS) mapping approach on a diversity panel of ~360 rice accessions. Multiple loci with small effects on shoot growth trajectory were identified, indicating a complex polygenic architecture. Natural variation for shoot growth dynamics was assessed in a subset …

Seed Yield Prediction Models Of Four Common Moist-Soil Plant Species In Texas, Daniel P. Collins, Warren C. Conway, Corey D. Mason, Jeffrey W. Gunnels

Faculty Publications

Seed production by moist-soil plant species often varies within and among managed wetlands and on larger landscapes. Quantifying seed production of moist-soil plants can be used to evaluate wetland management strategies and estimate wetland energetic carrying capacity, specifically for waterfowl. In the past, direct estimation techniques were used, but due to excessive personnel and time costs, other indirect methods have been developed. Because indirect seed yield models do not exist for moist-soil plant species in east-central or coastal Texas, we developed direct and indirect methods to model seed production on regional managed wetlands. In September 2004 and 2005, we collected …

Winter Wheat Variety Trial Archive, John Rickertsen, Robert Hall, Amir Ibrahim, Kevin Kirby, Thandiwe Nleya, Bruce Swan, Steve Kalsbeck, Bill Berzonsky, Nathan Mueller, Christopher Graham, Shaukat Ali, S. M. Hawks

SDSU Extension Publications Archive

Crop performance testing results are released annually through the activities of SDSU Extension and the South Dakota Agricultural Experiment Station at SDSU.

Succession; A Business Strategy, Jan Miller, Department Of Primary Industries And Regional Development, Western Australia

Farm Systems and Economics published reports

Plan, Prepare, Prosper - workshop

This document is part of the Plan, Prepare, Prosper program that was developed under the Pilot of Drought Reform Measures ('the pilot'), a joint initiative between the Australian Government and the Western Australian Government. The program aims to support farmers and rural communities to prepare for drought, climate variability and other challenges.

The Succession planning: A business strategy workshop will help participants to begin and maintain the succession planning process.

This workshop will guide you through the process of exploring, planning and enacting succession planning.

Please use this manual as a checklist to help you …

The Role Of Tre6p And Snrk1 In Maize Early Kernel Development And Events Leading To Stress-Induced Kernel Abortion, Samuel W. Bledsoe, Clémence Henry, Cara A. Griffiths, Matthew J. Paul, Regina Feil, John E. Lunn, Mark Stitt, L. Mark Lagrimini

Department of Agronomy and Horticulture: Faculty Publications

Background: Drought stress during flowering is a major contributor to yield loss in maize. Genetic and biotechnological improvement in yield sustainability requires an understanding of the mechanisms underpinning yield loss. Sucrose starvation has been proposed as the cause for kernel abortion; however, potential targets for genetic improvement have not been identified. Field and greenhouse drought studies with maize are expensive and it can be difficult to reproduce results; therefore, an in vitro kernel culture method is presented as a proxy for drought stress occurring at the time of flowering in maize (3 days after pollination). This method is used to …

Changes In Soil Microbial Communities After 10 Years Of Winter Wheat Cultivation Versus Fallow In An Organic-Poor Soil In The Loess Plateau Of China, Hui Tian, Hui Wang, Xiaoli Hui, Zhaohui Wang, Rhae A. Drijber, Jinshan Liu

Department of Agronomy and Horticulture: Faculty Publications

Agricultural management methods, such as cultivation or fallowing, have led to significant changes in soil fertility and hence, crop yield. Such changes may have stemmed from changes in soil microbial communities and associated biogeochemical processes. This phenomenon is particularly true in organic-poor soil in the Loess Plateau of China. In this study, we examined three existing soil management regimes as part of a 10-year field experiment and evaluated their effects on fungal and bacterial community structures by performing high-throughput 454 pyrosequencing. These management regimes were (i) fertilized winter wheat (Triticum aestivum L.) (FW), (ii) continuous natural fallow with weeds …

Mapping And Characterization Of The Fefe Gene That Controls Iron Uptake In Melon (Cucumis Melo L.), Raghuprakash Kastoori Ramamurthy, Brian M. Waters

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) deficiency in plants limits crop growth and productivity. Molecular mechanisms that plants use to sense and respond to Fe deficiency by coordinated expression of Feuptake genes are not fully understood. The C940-fe chlorotic melon (Cucumis melo) mutant known as fefe is unable to upregulate Fe-uptake genes, however, the FeFe gene had not been identified. In this study, we used two F₂ mapping populations to map and identify the FeFe gene as bHLH38, a homolog of subgroup Ib bHLH genes from Arabidopsis thaliana that are involved in transcriptional regulation of Feuptake genes in partnership with the …

Population Structure And Genetic Diversity Of Bromus Tectorum Within The Small Grain Production Region Of The Pacific Northwest, Nevin C. Lawrence, Amber L. Hauvermale, Amit Dhingra, Ian C. Burke

Department of Agronomy and Horticulture: Faculty Publications

Bromus tectorum L. is an invasive winter annual grass naturalized across the United States. Numerous studies have investigated B. tectorum population structure and genetics in the context of B. tectorum as an ecological invader of natural areas and rangeland. Despite the wealth of information regarding B. tectorum, previous studies have not focused on, or made comparisons to, B. tectorum as it persists in individual agroecosystems. The objectives of this study were to assess the genetic diversity and structure, the occurrence of generalist and specialist genotypes, and the influence of climate on distribution of B. tectorum sourced exclusively from within …

A Transcriptome‑Snp‑Derived Linkage Map Of Apios Americana (Potato Bean) Provides Insights About Genome Re‑Organization And Synteny Conservation In The Phaseoloid Legumes, Jugpreet Singh, Scott R. Kalberer, Vikas Belamkar, Teshale Assefa, Matthew N. Nelson, Andrew D. Farmer, William J. Blackmon, Steven B. Cannon

Department of Agronomy and Horticulture: Faculty Publications

Apios (Apios americana; “apios”), a tuberous perennial legume in the Phaseoleae tribe, was widely used as a food by Native Americans. Work in the last 40 years has led to several improved breeding lines. Aspects of the pollination biology (complex floral structure and tripping mechanism) have made controlled crosses difficult, and the previous reports indicated that the plant is likely primarily an outcrosser. We used a pseudo-testcross strategy to construct a genetic map specific to the maternal parent. The map was built using single-nucleotide polymorphism markers identified by comparing the expressed sequences of individuals in the mapping population …

The Trouble With Cover Crops: Farmers’ Experiences With Overcoming Barriers To Adoption, Gabrielle E. Roesch-Mcnally, Andrea D. Basche, J. G. Arbuckle, John C. Tyndall, Fernando E. Miguez, Troy Bowman, Rebecca Clay

Department of Agronomy and Horticulture: Faculty Publications

Cover crops are known to promote many aspects of soil and water quality, yet estimates find that in 2012 only 2.3% of the total agricultural lands in the Midwestern USA were using cover crops. Focus groups were conducted across the Corn Belt state of Iowa to better understand how farmers confront barriers to cover crop adoption in highly intensive agricultural production systems. Although much prior research has focused on analyzing factors that help predict cover crop use on farms, there is limited research on how farmers navigate and overcome field-level (e.g. proper planting of a cover crop) and structural barriers …

Genetic Diversity Of Danthonia Spicata (L.) Beauv. Based On Genomic Simple Sequence Repeat Markers, Chandra S. Thammina, Keenan Amundsen, Shaun B. Bushman, Matthew H. Kramer, Scott E. Warnke

Department of Agronomy and Horticulture: Faculty Publications

Danthonia spicata (L.) Beauv., commonly known as poverty oatgrass, is a perennial bunch-type grass native to North America. D. spicata is often found in low input turfgrass areas on the East Coast of the United States and has potential for development as a new native low input turfgrass species. Roche 454 sequenced randomly sheared genomic DNA reads of D. spicata were mined for SSR markers using the MIcroSAtellite identification tool. A total of 66,553 singlet sequences (approximately 37.5 Mbp) were examined, and 3454 SSR markers were identified. Trinucleotide motifs with greater than six repeats and possessing unique PCR priming sites …

Molecular And Phenotypic Characterization Of Transgenic Wheat And Sorghum Events Expressing The Barley Alanine Aminotransferase, Pamela A. Peña, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Ismail M. Dweikat, Madhavan Soundararajan, Tom Clemente

Department of Agronomy and Horticulture: Faculty Publications

Main conclusion — The expression of a barley alanine aminotransferase gene impacts agronomic outcomes in a C3 crop, wheat.

The use of nitrogen-based fertilizers has become one of the major agronomic inputs in crop production systems. Strategies to enhance nitrogen assimilation and flux in planta are being pursued through the introduction of novel genetic alleles. Here an Agrobacterium-mediated approach was employed to introduce the alanine aminotransferase from barley (Hordeum vulgare), HvAlaAT, into wheat (Triticum aestivum) and sorghum (Sorghum bicolor), regulated by either constitutive or root preferred promoter elements. Plants harboring the transgenic …

Metabolism Of 2,4-Dichlorophenoxyacetic Acid Contributes To Resistance In A Common Waterhemp (Amaranthus Tuberculatus) Population, Marcelo R. A. Figueiredo, Lacy J. Leibhart, Zachary J. Reicher, Patrick J. Tranel, Scott J. Nissen, Philip Westra, Mark L. Bernards, Greg R. Kruger, Todd A. Gaines, Mithila Jugulam

Department of Agronomy and Horticulture: Faculty Publications

BACKGROUND: Synthetic auxins such as 2,4-D have been widely used for selective control of broadleaf weeds since the mid-1940s. In 2009, an Amaranthus tuberculatus (common waterhemp) population with 10-fold resistance to 2,4-D was found in Nebraska, USA. The 2,4-D resistance mechanism was examined by conducting [¹⁴C] 2,4-D absorption, translocation and metabolism experiments.

RESULTS: No differences were found in 2,4-D absorption or translocation between the resistant and susceptible A. tuberculatus. Resistant plants metabolized [¹⁴C] 2,4-D more rapidly than did susceptible plants. The half-life of [¹⁴C] 2,4-D in susceptible plants was 105 h, compared to …

Multisite Evaluation Of Apex For Water Quality: Ii. Regional Parameterization, Nathan O. Nelson, Claire Baffaut, John A. Lory, G.M.M.M. Anomaa Senaviratne, Ammar B. Bhandari, Ranjith P. Udawatta, Daniel W. Sweeney, Matt J. Helmers, Mike W. Van Liew, Antonio P. Mallarino, Charles S. Wortmann

Department of Agronomy and Horticulture: Faculty Publications

Phosphorus (P) Index assessment requires independent estimates of long-term average annual P loss from fields, representing multiple climatic scenarios, management practices, and landscape positions. Because currently available measured data are insufficient to evaluate P Index performance, calibrated and validated process-based models have been proposed as tools to generate the required data. The objectives of this research were to develop a regional parameterization for the Agricultural Policy Environmental eXtender (APEX) model to estimate edgeof- field runoff, sediment, and P losses in restricted-layer soils of Missouri and Kansas and to assess the performance of this parameterization using monitoring data from multiple sites …