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Full-Text Articles in Physiology
Development Of A High Throughput, Whole Organism Assay For Studying Metabolic Capacity In Crassostrea Virginica, Avuntaura Gulledge
Development Of A High Throughput, Whole Organism Assay For Studying Metabolic Capacity In Crassostrea Virginica, Avuntaura Gulledge
Honors College
Genetic improvement of commercial lines has been a key component in the increased production of eastern oysters (Crassostrea virginica). One form of genetic improvement, induced triploidy, is commonly used to produce sterile oysters with faster growth and higher meat quality. However, the details of the physiological advantage provided by triploidy are poorly understood. Whole animal respiration is an important indicator of an individual’s capacity to meet increased energetic demands for growth and stress response. I have developed an assay for the Agilent’s XFe96 Extracellular Flux Analyzer to measure metabolic capacity (i.e., rates of basal, maximal, mitochondrial, and non-mitochondrial oxygen consumption) …
Beyond Cortisol: Indicators Of Stress And Negative Feedback In Plasma And Blubber Of Marine Mammals, Jessica Avalos
Beyond Cortisol: Indicators Of Stress And Negative Feedback In Plasma And Blubber Of Marine Mammals, Jessica Avalos
University of the Pacific Theses and Dissertations
Marine mammals play an important role in ecosystem stability. However, anthropogenic activity is compounding pressure on many already vulnerable populations. A potential consequence of anthropogenic disturbance is physiological stress, which can impact metabolism, immunity, and reproduction, especially if it occurs repeatedly. Previous studies on marine mammals have focused on acute stress, but the impacts of repeated stress are poorly understood. Due to its accessibility on land during haul-outs, the northern elephant seal (Mirounga angustirostris) is a good system in which to study the effects of stress in marine mammals. Stress stimulates the release of glucocorticoid hormones, primarily cortisol. Elevated cortisol …
Effects Of Intertidal Position On Metabolism And Behavior In The Acorn Barnacle, Balanus Glandula, Kali Horn
Effects Of Intertidal Position On Metabolism And Behavior In The Acorn Barnacle, Balanus Glandula, Kali Horn
Master's Theses
The intertidal zone is characterized by persistent, tidally-driven fluctuations in both abiotic (e.g., temperature, [O2], salinity) and biotic (e.g., food availability, predation) conditions, which makes this a very physiologically challenging habitat for resident organisms. The magnitude and degree of variability of these environmental stressors differs between intertidal zones, with the most extreme physiological stress often being experienced by organisms in the high intertidal. Given that many of the fluctuating conditions in this environment are primary drivers of metabolic rate (e.g., temperature, [O2], food availability), we hypothesized that sessile conspecifics residing in different tidal zones would exhibit …
Effect Of Oxygen-Limiting Tidal Conditions On Muscle Metabolism And Structure In The Giant Acorn Barnacle, Balanus Nubilus, Katie O. Grady
Effect Of Oxygen-Limiting Tidal Conditions On Muscle Metabolism And Structure In The Giant Acorn Barnacle, Balanus Nubilus, Katie O. Grady
Master's Theses
Crustacean muscle fibers are some of the largest cells in the animal kingdom, with fiber diameters in the giant acorn barnacle (Balanus nubilus) exceeding 3 mm. Sessile animals with extreme muscle sizes and that live in the hypoxia-inducing intertidal zone – like B. nubilus – represent ideal models for probing the effects of oxygen limitation on muscle cells. We investigated changes in metabolism and structure of B. nubilus muscle in response to: normoxic immersion, anoxic immersion, or air emersion, for acute (6h) or chronic (6h exposures twice daily for 2wks) time periods. Following exposure, we immediately measured hemolymph …