Physiology Commons^™

The Endogenous Molecular Clock Orchestrates The Temporal Separation Of Substrate Metabolism In Skeletal Muscle, Brian A. Hodge, Yuan Wen, Lance A. Riley, Xiping Zhang, Jonathan H. England, Brianna D. Harfmann, Elizabeth A. Schroder, Karyn A. Esser

Physiology Faculty Publications

BACKGROUND: Skeletal muscle is a major contributor to whole-body metabolism as it serves as a depot for both glucose and amino acids, and is a highly metabolically active tissue. Within skeletal muscle exists an intrinsic molecular clock mechanism that regulates the timing of physiological processes. A key function of the clock is to regulate the timing of metabolic processes to anticipate time of day changes in environmental conditions. The purpose of this study was to identify metabolic genes that are expressed in a circadian manner and determine if these genes are regulated downstream of the intrinsic molecular clock by …

Proteomic Analysis Of Media From Lung Cancer Cells Reveals Role Of 14-3-3 Proteins In Cachexia, Julie B. Mclean, Jennifer S. Moylan, Erin M. Wolf Horrell, Francisco H. Andrade

Physiology Faculty Publications

Aims: At the time of diagnosis, 60% of lung cancer patients present with cachexia, a severe wasting syndrome that increases morbidity and mortality. Tumors secrete multiple factors that contribute to cachectic muscle wasting, and not all of these factors have been identified. We used Orbitrap electrospray ionization mass spectrometry to identify novel cachexia-inducing candidates in media conditioned with Lewis lung carcinoma cells (LCM).

Results: One-hundred and 58 proteins were confirmed in three biological replicates. Thirty-three were identified as secreted proteins, including 14-3-3 proteins, which are highly conserved adaptor proteins known to have over 200 binding partners. We confirmed the …

The Role Of Mmp-2, 9, And 13, In The Regulation Of Skeltal Muscle Hypertrophy Following Functional Overload, Reilly Fitzpatrick

Summer Research

PURPOSE: Skeletal muscle is the most abundant tissue in vertebrates that functions primarily to generate locomotion, and exhibits a high degree of structural and functional plasticity that is largely dependent on the level of activity placed on it. Recent studies have shown that a family of enzymes known as matrix metalloproteinases (MMPs) play an important role in regulating this plasticity. Specifically, MMP-2 and MMP-9 degrade components of the extracellular matrix (ECM) surrounding muscle fiber during periods of growth and repair. However, the mechanisms by which these MMPs mediate this response and how each contributes to muscle repair and remodeling remains …