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- Actin polymerization (1)
- Beta subunits (1)
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Articles 1 - 3 of 3
Full-Text Articles in Physiology
An Extracellular Domain Of The Accessory Beta1 Subunit Is Required For Modulating Bk Channel Voltage Sensor And Gate, Aleksandra Gruslova, Iurii Semenov, Bin Wang
An Extracellular Domain Of The Accessory Beta1 Subunit Is Required For Modulating Bk Channel Voltage Sensor And Gate, Aleksandra Gruslova, Iurii Semenov, Bin Wang
Bioelectrics Publications
A family of tissue-specific auxiliary beta subunits modulates large conductance voltage- and calcium-activated potassium (BK) channel gating properties to suit their diverse functions. Paradoxically, beta subunits both promote BK channel activation through a stabilization of voltage sensor activation and reduce BK channel openings through an increased energetic barrier of the closed-to-open transition. The molecular determinants underlying beta subunit function, including the dual gating effects, remain unknown. In this study, we report the first identification of a beta1 functional domain consisting of Y74, S104, Y105, and I106 residues located in the extracellular loop of beta1. These amino acids reside within two …
In Vitro Measurements Of Tracheal Constriction Using Mice, Iurii Semenov, Jeremiah T. Herlihy, Robert Brenner
In Vitro Measurements Of Tracheal Constriction Using Mice, Iurii Semenov, Jeremiah T. Herlihy, Robert Brenner
Bioelectrics Publications
Transgenic and knockout mice have been powerful tools for the investigation of the physiology and pathophysiology of airways(1,2). In vitro tensometry of isolated tracheal preparations has proven to be a useful assay of airway smooth muscle (ASM) contractile response in genetically modified mice. These in vitro tracheal preparations are relatively simple, provide a robust response, and retain both functional cholinergic nerve endings and muscle responses, even after long incubations. Tracheal tensometry also provides a functional assay to study a variety of second messenger signaling pathways that affect contraction of smooth muscle. Contraction in trachea is primarily mediated by parasympathetic, cholinergic …
Electric Field Exposure Triggers And Guides Formation Of Pseudopod-Like Blebs In U937 Monocytes, Mikhail A. Rassokhin, Andrei G. Pakhomov
Electric Field Exposure Triggers And Guides Formation Of Pseudopod-Like Blebs In U937 Monocytes, Mikhail A. Rassokhin, Andrei G. Pakhomov
Bioelectrics Publications
We describe a new phenomenon of anodotropic pseudopod-like blebbing in U937 cells stimulated by nanosecond pulsed electric field (nsPEF). In contrast to "regular," round-shaped blebs, which are often seen in response to cell damage, pseudopod-like blebs (PLBs) formed as longitudinal membrane protrusions toward anode. PLB length could exceed the cell diameter in 2 min of exposure to 60-ns, 10-kV/cm pulses delivered at 10-20 Hz. Both PLBs and round-shaped nsPEF-induced blebs could be efficiently inhibited by partial isosmotic replacement of bath NaCl for a larger solute (sucrose), thereby pointing to the colloid-osmotic water uptake as the principal driving force for bleb …