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Full-Text Articles in Physiology
Assembly Of Α4Β2 Nicotinic Acetylcholine Receptors Assessed With Functional Fluorescently Labeled Subunits: Effects Of Localization, Trafficking, And Nicotine-Induced Upregulation In Clonal Mammalian Cells And In Cultured Midbrain Neurons, Raad Nashmi, Mary E. Dickinson, Sheri Mckinney, Mark Jareb, Cesar Labarca, Scott E. Fraser E. Fraser, Henry A. Lester
Assembly Of Α4Β2 Nicotinic Acetylcholine Receptors Assessed With Functional Fluorescently Labeled Subunits: Effects Of Localization, Trafficking, And Nicotine-Induced Upregulation In Clonal Mammalian Cells And In Cultured Midbrain Neurons, Raad Nashmi, Mary E. Dickinson, Sheri Mckinney, Mark Jareb, Cesar Labarca, Scott E. Fraser E. Fraser, Henry A. Lester
Biology Faculty Publications
Fura-2 recording of Ca2+ influx was used to show that incubation in 1 μM nicotine (2–6d) upregulates several pharmacological components of acetylcholine (ACh) responses in ventral midbrain cultures, including a MLA-resistant, DHβE-sensitive component that presumably corresponds to α4β2 receptors. To study changes in α4β2 receptor levels and assembly during this upregulation, we incorporated yellow and cyan fluorescent proteins (YFPs and CFPs) into the α4 or β2 M3–M4 intracellular loops, and these subunits were coexpressed in human embryonic kidney (HEK) 293T cells and cultured ventral midbrain neurons. The fluorescent …
Rapping To Review: A Novel Strategy To Engage Students And Summarize Course Material, Maureen T. Knabb
Rapping To Review: A Novel Strategy To Engage Students And Summarize Course Material, Maureen T. Knabb
Biology Faculty Publications
No abstract provided.