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Articles 1 - 23 of 23
Full-Text Articles in Physiology
Igf-1 Reduce Protein O-Glcnacylation Via Ampk Activation In H9c2 Cardiomyoblast Cultured In High Glucose Media, Andres Medina, Lizbeth Trevino, Alejandra Salinas, Yadira Chaires, Erick Rodriguez, Genaro Antonio Ramirez-Correa
Igf-1 Reduce Protein O-Glcnacylation Via Ampk Activation In H9c2 Cardiomyoblast Cultured In High Glucose Media, Andres Medina, Lizbeth Trevino, Alejandra Salinas, Yadira Chaires, Erick Rodriguez, Genaro Antonio Ramirez-Correa
Research Colloquium
Background: Diabetes is a disease characterized by the increase in the serum glucose concentration. Therefore, this glucose excess shunts into the hexosamine biosynthetic pathway producing UDP-GlcNAc, which can be transfer to serine residues and modify the activity of different proteins, including contractile proteins in cardiomyocytes, decreasing their function. Here we show the effect of IGF-1 treatment on this posttranslational modification.
Methods: H9c2 cell line were cultured in DMEM media, 10% FBS, at 80% of confluence. Cells were incubated in high-glucose (30 mM) in the presence or absence of 10 µmol/L of IGF-1 (HG and HG+IGF-1). As control groups, we used …
Endothelial Cell Nrf2-Ko Attenuates Endothelial Function And Skeletal Muscle Antioxidant Capacity, Elizabeth J. Pekas, Cody P. Anderson, Michael F. Allen, Kollyn B. Jallen, Dong Gun Jin, Andres Benitez-Albiter, Christian D. Sanchez Tato, Lie Gao, Neha Dhyani, Tara L. Rudebush, Irving H. Zucker, Song-Young Park
Endothelial Cell Nrf2-Ko Attenuates Endothelial Function And Skeletal Muscle Antioxidant Capacity, Elizabeth J. Pekas, Cody P. Anderson, Michael F. Allen, Kollyn B. Jallen, Dong Gun Jin, Andres Benitez-Albiter, Christian D. Sanchez Tato, Lie Gao, Neha Dhyani, Tara L. Rudebush, Irving H. Zucker, Song-Young Park
UNO Student Research and Creative Activity Fair
INTRODUCTION: Endothelial cells line the inner surface of blood vessels and play a major role in modulating blood flow and gas exchange. Endothelial dysfunction is thought to be a contributor to cardiovascular disease development, and it is well-accepted that excessive reactive oxygen species (harmful molecules) likely contribute to endothelial dysfunction. The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) is considered the master regulator of cellular protection in response to elevated reactive oxygen species. Therefore, Nrf2 may be a potential therapeutic target to protect against endothelial dysfunction. However, the roles of endothelial cell-specific Nrf2 on endothelial function are not …
Impaired Hydrogen Sulfide Protein Expression In Patients With Peripheral Artery Disease, Dong Gun Jin, Kollyn B. Jallen, Liz Pekas, Song-Young Park
Impaired Hydrogen Sulfide Protein Expression In Patients With Peripheral Artery Disease, Dong Gun Jin, Kollyn B. Jallen, Liz Pekas, Song-Young Park
UNO Student Research and Creative Activity Fair
INTRODUCTION: Hydrogen sulfide (H2S) is a gaseous signaling molecule that serves various roles in the vasculature, such as upregulating angiogenesis, vascular smooth muscle relaxation, protecting endothelial function, and regulating redox balance. Despite H2S’s positive impacts on vascular homeostasis, it is important to note that its actions depend on its concentrations. At high concentrations, H2S has been reported to increase oxidative stress damage, such as oxidation of cysteine residues and lipid peroxidation. This may indicate that H2S may act as a ‘double-edged sword’ in the field of vascular physiology. Peripheral artery disease (PAD) …
Prevention Of Doxorubicin-Induced Cardiotoxicity By A Coumestan Analogue, Psoralidin, Mujtaba H. Shah, Myia Aiges, Kota V. Ramana
Prevention Of Doxorubicin-Induced Cardiotoxicity By A Coumestan Analogue, Psoralidin, Mujtaba H. Shah, Myia Aiges, Kota V. Ramana
Annual Research Symposium
Natural phenolic compound Psoralidin limits cardiotoxicity associated with Doxorubicin
Assessing The Role Of Integrins In Contact Guidance By Substratum Topography, Robin S. Hong
Assessing The Role Of Integrins In Contact Guidance By Substratum Topography, Robin S. Hong
Undergraduate Student Research Internships Conference
In this project, the effect of blocking integrin subunits on contact guidance of human periodontal ligament fibroblasts were assessed. Imaging was gathered through Timelapse imaging of live cells as well as immunohistochemistry of fixed cells at designated timepoints.
Carbonyl Iron Vs Ferrous Sulfate On Blood Iron Markers In Male Division Iii Cross-Country Athletes, Sam Gunter
Carbonyl Iron Vs Ferrous Sulfate On Blood Iron Markers In Male Division Iii Cross-Country Athletes, Sam Gunter
Student Scholar Showcase
Competitive distance runners are often predisposed to developing iron deficiency. This study aimed to determine if carbonyl iron was more effective at maintaining blood iron markers and minimizing overall fatigue and GI stress than ferrous sulfate. In this randomized, independent groups study, 7 male, division III cross-country athletes were supplemented with either carbonyl iron or ferrous sulfate for 6 weeks. Blood hemoglobin, hematocrit, subjective GI distress, subjective fatigue, and relative exertion of recent training were assessed at baseline, 3 and 6 weeks. Results will be analyzed via a detailed analysis of variance to determine significant differences in outcome measures. Statistical …
Temperature Changes Seen In Lower Extremities After Cpn Local Anesthetic Block, Lora Asberry
Temperature Changes Seen In Lower Extremities After Cpn Local Anesthetic Block, Lora Asberry
Symposium of Student Scholars
Many older patients often suffer from walking issues such as Drop Foot. Drop Foot is caused by the malfunction of nerves in the foot, resulting in the loss of control of the front foot muscle. Within all of our patients, there has been a common fibular palsy, caused by the entrapment of the peroneal nerve. Due to this, they cannot lift up their foot. Some cases are permanent, while others are temporary. In the temporary cases, we have indicated a Phoenix Sign. The Phoenix Sign indicates that a nerve, presumed to be dead, has the capability to be recessed back …
Age-Related Reduction In High-Velocity Power And Myofiber Morphology And Composition, Ben Dalton
Age-Related Reduction In High-Velocity Power And Myofiber Morphology And Composition, Ben Dalton
Symposium of Student Scholars
Power is diminished more dramatically at higher contraction velocities in older adults. It has been suggested that this may reflect age-related changes in single myofiber morphology or composition. PURPOSE: To examine power, muscle activation, and single myofiber morphology and composition between young (YM) and older (OM) males. METHODS: Power, or torque × velocity, was recorded during isokinetic knee extensions at 60°·s-1 and 180°·s-1 in healthy, untrained YM (n=15; 20.7±2.2 yrs) and OM (n=15; 71.6±3.9 yrs). The relative increase in power from 60°·s-1 to 180°·s-1 was recorded for each participant. Electromyography amplitude of the vastus lateralis (VL) …
Validation Of A Novel Trpm8 Knockout Mouse Model, Bilan Mohamed
Validation Of A Novel Trpm8 Knockout Mouse Model, Bilan Mohamed
Thinking Matters Symposium
Recent studies suggest that the use of thermoregulatory treatments that affect brown fat may help curb obesity. However it is unknown how these treatments may affect bone homeostasis. Our work has focused on the transient receptor potential melastatin (TRPM8) protein, which is responsible for detecting colder temperatures in sensory neurons. Previous work within the Motyl laboratory has found that Trpm8 plays a role in bone acquisition. Mice with a global deletion of the Trpm8 gene have reduced trabecular bone volume fraction due to reduced bone formation by osteoblasts. However, it is unclear whether sensory neuron or osteoblast-mediated expression of Trpm8 …
Mathematical Modelling Of Temperature Effects On The Afd Neuron Of Caenorhabditis Elegans, Zachary Mobille, Rosangela Follmann, Epaminondas Rosa
Mathematical Modelling Of Temperature Effects On The Afd Neuron Of Caenorhabditis Elegans, Zachary Mobille, Rosangela Follmann, Epaminondas Rosa
Annual Symposium on Biomathematics and Ecology Education and Research
No abstract provided.
Switching Off The Pancreatic Islet: Complex Network Analysis Of Multicellular Clusters, Janita Patwardhan
Switching Off The Pancreatic Islet: Complex Network Analysis Of Multicellular Clusters, Janita Patwardhan
Biology and Medicine Through Mathematics Conference
No abstract provided.
Age Dependent Regulation Of Cardiac Sodium Channel Gain Of Function, Madison Nowak, David Ryan King, Steven Poelzing, Seth Weinberg
Age Dependent Regulation Of Cardiac Sodium Channel Gain Of Function, Madison Nowak, David Ryan King, Steven Poelzing, Seth Weinberg
Biology and Medicine Through Mathematics Conference
No abstract provided.
Calcium Signaling In The Sperm Head, Julie Simons, Lisa Fauci
Calcium Signaling In The Sperm Head, Julie Simons, Lisa Fauci
Annual Symposium on Biomathematics and Ecology Education and Research
No abstract provided.
Disruption Of Synchronous Behavior In Pancreatic Islets Via Hub Cells, Janita Patwardhan
Disruption Of Synchronous Behavior In Pancreatic Islets Via Hub Cells, Janita Patwardhan
Biology and Medicine Through Mathematics Conference
No abstract provided.
Neuronal Degeneration And Short-Term Memory Impairment After Tbi, Obiamaraije Igwe
Neuronal Degeneration And Short-Term Memory Impairment After Tbi, Obiamaraije Igwe
Posters-at-the-Capitol
Traumatic brain injury (TBI) was associated with impaired short-term memory with causes of vehicle accidents and falls. Protein plaques containing fibrinogen (Fg), are associated with memory loss. After TBI, Fg in blood was higher than normal (>~2 mg/ml), which resulted in increased Fg in extravascular space. Therefore, Fg bonded to its endothelial receptor intercellular adhesion molecule-1 (ICAM-1). Fg then interacted with cellular prion protein (PrPC), which had a strong effect on the loss of memory and cognition. Mechanisms of Fg and PrPC complex formation and its functional implication are not known. This present study tested the level of Fg-PrPC …
Heads And Tails, Julie Simons
Heads And Tails, Julie Simons
Annual Symposium on Biomathematics and Ecology Education and Research
No abstract provided.
Capacitive Memory Alters Alternans And Spontaneous Activity In A Minimal Cardiomyocyte Model, Tien Comlekoglu, Seth H. Weinberg
Capacitive Memory Alters Alternans And Spontaneous Activity In A Minimal Cardiomyocyte Model, Tien Comlekoglu, Seth H. Weinberg
Biology and Medicine Through Mathematics Conference
No abstract provided.
Using Fluorescence Microscopy To Identify A Potential New Treatment For Heart Failure, Ryan D. Himes
Using Fluorescence Microscopy To Identify A Potential New Treatment For Heart Failure, Ryan D. Himes
Scholar Week 2016 - present
Cardiac glycosides have been used to treat heart failure for centuries, but they have a narrow therapeutic window, as they inhibit their target receptor nearly irreversibly. Overdoses can lead to arrhythmias. Phospholemman is a natural inhibitor of the same target as cardiac glycosides. It is possible that mutating phospholemman could achieve the same therapeutic benefit, while allowing cells to reverse the inhibition and thereby avoid an arrhythmia. I used fluorescence microscopy to screen candidate phospholemman mutants and identify one that binds more avidly to its target than the naturally occurring phospholemman. This mutant, L30A, caused similar effects as cardiac glycosides …
Antiproliferative Effects Of Hibernating American Bullfrog (Rana Catesbeiana) Blood Plasma On Thp-1 Cells, Sean Robbins
Antiproliferative Effects Of Hibernating American Bullfrog (Rana Catesbeiana) Blood Plasma On Thp-1 Cells, Sean Robbins
Annual Graduate Student Symposium
Hibernation is an incredible adaptation that allows an organism to go dormant during the winter, when food is scarce. Much is unknown physiologically about hibernation, and most current research comes from mammalian models. During hibernation, the cells of mammals are impeded from going through mitosis. 13-lined ground squirrels (Ictidomys tridecemlineatus) and woodchucks (Marmota monax) have a protein, alpha-2-macroglobulin, in their blood plasma, which has significant antiproliferative effects on spleen cells. The goal of my study was to determine if an exothermic organism, the American Bullfrog (Rana catesbeiana), also actively suppresses mitosis while hibernating and if so, determine if it is …
Maintenance Of The Ph Gradient In The Gastric Mucus Layer., Owen Lewis
Maintenance Of The Ph Gradient In The Gastric Mucus Layer., Owen Lewis
Annual Symposium on Biomathematics and Ecology Education and Research
No abstract provided.
Understanding Adipocyte Dynamics Through Mathematical Modeling, Katrina Johnson, Fred Adler
Understanding Adipocyte Dynamics Through Mathematical Modeling, Katrina Johnson, Fred Adler
Annual Symposium on Biomathematics and Ecology Education and Research
No abstract provided.
Hill's Diagrammatic Method And Reduced Graph Powers, Gregory D. Smith, Richard Hammack
Hill's Diagrammatic Method And Reduced Graph Powers, Gregory D. Smith, Richard Hammack
Biology and Medicine Through Mathematics Conference
No abstract provided.
Single Nucleotide Polymorphisms In Cldn14 And Smoc1 Affecting Bone Mineral Density Influence Other Musculoskeletal Traits, Christopher Payette, Courtney Sprouse, Cara Goerlich, Heather A. Gordish-Dressman, Thomas Lynch, Heather Flynn, Leticia M. Ryan, Eric P. Hoffman, Monica J. Hubal, Paul D. Thompson, Theodore J. Angelopoulos, Paul M. Gordon, Niall M. Moyna, Linda S. Pescatello, Paul S. Visich, Robert F. Zoeller, Laura L. Tosi
Single Nucleotide Polymorphisms In Cldn14 And Smoc1 Affecting Bone Mineral Density Influence Other Musculoskeletal Traits, Christopher Payette, Courtney Sprouse, Cara Goerlich, Heather A. Gordish-Dressman, Thomas Lynch, Heather Flynn, Leticia M. Ryan, Eric P. Hoffman, Monica J. Hubal, Paul D. Thompson, Theodore J. Angelopoulos, Paul M. Gordon, Niall M. Moyna, Linda S. Pescatello, Paul S. Visich, Robert F. Zoeller, Laura L. Tosi
GW Research Days 2016 - 2020
Background: A recent genome-wide association study (GWAS) identified novel genes influencing bone mineral density (BMD). This three stage GWAS identified two novel loci: rs227425 in the SPARC-Related Modular Calcium Binding 1 gene (SMOC1) was significantly associated with BMD and rs170183 in the claudin 14 (CLDN14) gene was significantly associated with BMD in females.
Objective: The purpose of this study was to determine if two novel single nucleotide polymorphisms (SNPs) known to affect BMD are associated with other musculoskeletal traits.
Methods/Design :The Bone Health Cohort consists of 150 African-American participants enrolled at Children’s National Health System as part of …