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Full-Text Articles in Physiology
Cholinergic Neurons Regulate And Utilize Gdnf Secreted By C2c12 Skeletal Muscle Cells In Culture, John-Mary Vianney, John Spitsbergen
Cholinergic Neurons Regulate And Utilize Gdnf Secreted By C2c12 Skeletal Muscle Cells In Culture, John-Mary Vianney, John Spitsbergen
Biological Sciences Faculty and Graduate Student Research
Glialcell line-derived neurotrophicfactor (GDNF) has been regarded as a potent survival factor for a subpopulation of neurons. It has been shown that GDNF expression is upregulated in skeletal muscle of patients with early stage of neuromuscular diseases such as amyotrophic lateral sclerosis (ALS). Previous results from our laboratory showed that neural cells regulate GDNF secretion by skeletal muscle; non-innervated skeletal muscle appear to secrete more GDNF compared to innervated skeletal muscle. Two aims were examined in the current study. First, to examine whether neural cells inhibit GDNF through acetylcholine release. Second was to examine whether differentiated NG108-15 neural cells secrete …
Changes In Gdnf Protein Content Of Skeletal Muscle Following Involuntary Exercise Are Long Lived, Kyle R. Kinnell, Monica J. Czarnopys, John Spitsbergen
Changes In Gdnf Protein Content Of Skeletal Muscle Following Involuntary Exercise Are Long Lived, Kyle R. Kinnell, Monica J. Czarnopys, John Spitsbergen
Biological Sciences Faculty and Graduate Student Research
Glial-cell line derived neurotrophic factor (GDNF) is a neurotrophic factor produced by skeletal muscle. GDNF has been shown to be potent survival factor for motor neurons. We have previously shown that GDNF content in skeletal muscle is altered following exercise and GDNF levels 24 hours after the last bout of exercise are significantly different than those measured immediately after exercise. We sought to determine how long after exercise do GDNF protein levels remain altered. We removed extensor digitorum longus (EDL) and Soleus (SOL), 24 hr, 72 hr and 1 week following the last bout of involuntary exercise. Muscles were processed …
Exposure Of Nervous System Cells To Polychlorinated Biphenyls (Pcbs) Results In Significant Alterations In Levels Of Expression Of Neurotrophic Factors, Charles F. Ide, Jay C. Means, Trisha M. Basford, John Spitsbergen
Exposure Of Nervous System Cells To Polychlorinated Biphenyls (Pcbs) Results In Significant Alterations In Levels Of Expression Of Neurotrophic Factors, Charles F. Ide, Jay C. Means, Trisha M. Basford, John Spitsbergen
Biological Sciences Faculty and Graduate Student Research
Toxic insult by PCBs results in learning and memory deficits in humans. Alterations in expression of neurotrophic factors and/or their receptors have been linked to changes in cognition. How PCBs affect cognition is not known. We propose that PCBs affect cognition by altering neurotrophic factor expression or effects. We exposed cultured C6 glial cells in medium containing PCB (Aroclor 1254 (10ppm)). Control cells were treated with DMSO or regular medium. Cells were incubated at 37o C for up to 72 hours. Medium samples were taken at 6hr, 24hr, 48hr, and 72hr intervals. Enzyme-linked immunosorbent assays (ELISA) were used to determine …