Physiology Commons^™

Effect Of Insulin-Like Growth Factor-1 (Igf-1) On The Expression Of Nfκb And Cflip In Bovine Granulosa Cells, Samantha Kathleen Docos

Student Research Projects

Infertility, often attributed to follicular atresia, is a growing problem in the agricultural industry. Programmed cell death, also known as apoptosis, is a contributing factor of follicular atresia. It occurs in both the granulosa cells and the oocyte that comprise ovarian follicles. Here, mechanisms influencing the process of apoptosis, via the death receptor Fas, were explored using bovine granulosa cells (bGCs) because Fas-induced apoptosis is a plausible mechanism of follicular atresia. Cell culture techniques, optimized for bGCs, were developed and used throughout the current study. In brief, cultures of bGCs were exposed to Fas ligand (100ng/mL) for 24 hrs. This …