Pharmacology Commons^™

Nanofabrication For Precision Vaccinology, Karen Zagorski

Theses & Dissertations

Vaccines are among the most effective and safe therapeutics known to mankind. Historically the approach to the creation of new vaccines has been dominated by empirical research, trial, and error. This thesis focuses on the more modern, rational approaches to vaccinology based on the current understanding of the inner workings of the immune system. We used nanotechnology for the generation of novel immunogens, addressed several challenges, and characterized the immune responses.

The preparation, characterization, and troubleshooting of several vaccines against dimeric amyloid beta are covered in chapters 4-6. Despite being ultimately unsuccessful, this part provided a deeper understanding of the …

A Case Of Transverse Myelitis Secondary To Covid-19 Infection, Rahul Borra, Neal T. Patel, Raheel Shaikh, Mayur S. Parmar, Sujatha Borra

HPD Articles

Infection with COVID-19 (SARS-CoV-2) is associated with a variety of generalized and specific symptoms, including neurological complications of both the peripheral and central nervous systems. In this case report, we present the case of a previously healthy 55-year-old woman who was diagnosed with transverse myelitis following a previous infection with COVID-19. MRI showed progressive demyelination of the cervical and thoracic spinal cord, and cerebrospinal fluid (CSF) showed increased levels of protein and red blood cells and no markers of infection, including negative polymerase chain reaction (PCR) for COVID-19 antibodies. The patient was treated with a course of methylprednisolone, multiple treatments …

Investigation Of Human N-Acetyltransferases (Nat1 And Nat2) Genetic Polymorphisms In Susceptibility To Aromatic Amine And Alkylaniline Genotoxicity., Mariam Habil

Electronic Theses and Dissertations

Arylamine N-acetyltransferases, NAT1 and NAT2, catalyze the detoxification and/or activation of carcinogens. Single nucleotide polymorphisms or SNPs result in different human NAT1 and NAT2 genotypes. We hypothesize that different NATs alleles will impact levels of N-acetylation, HPRT mutations, DNA damage and oxidative stress induced by carcinogens. NER-deficient Chinese hamster ovary (CHO) cells transfected with human CYP1A2 and NAT1*4, NAT1*14B, NAT2*4, NAT2*5B or NAT2*7B have been used to investigate N-acetylation of benzidine, β-naphthylamine (BNA), and 4, 4’-methylene bis (2-chloroaniline) (MOCA) as aromatic amines and 3,4-dimethylaniline (3,4-DMA) as one of alkylanilines and their associated toxicity. Our …

The Impact Of Pharmacogenetics On Oral Fluid Steady State Drug Testing Results, Aliya D. Lewis

Student Theses

Pharmacogenetics refers to the relationship between phenotypic variation in metabolism and response to certain drugs. The interface of pharmacogenetics and forensic toxicology is not well explored. A major component of the opioid metabolic pathway has to do with a family of cytochromes called cytochrome P450 (CYP450). CYP450 refers to a general family of enzymes capable of catalyzing the oxidative biotransformation of lipophilic substances, CYPs are further categorized into families and sub-families (Roller, 2015; Zanger & Schwab, 2013). The gene CYP2D6 belongs to the CYP2 family which is responsible for the biotransformation of xenobiotics. This study looked at the effect of …

Morphine-Induced Tolerance Is Reduced By The Consumption Of A Ketogenic Diet, But Not A High Fat/High Carbohydrate Diet, Nina Beltran

Open Access Theses & Dissertations

Opioid drugs like morphine are used medicinally for pain relief, but also have abuse liability that can lead to opioid use disorder (OUD), which contributes to nearly 70% of drug overdose deaths in the United States. Another rising health concern is the global obesity epidemic, and new evidence suggests that obesity and OUD might converge to impact individuals. For example, high body mass index (BMI) is associated with greater reports of pain among women. Further, opioid overdose risk is increased among individuals diagnosed with obesity, suggesting a potential relationship that might convey enhanced vulnerability among some populations. Given the high …

Acknowledgment Of Reviewers

Journal of Food and Drug Analysis

No abstract provided.

Inorganic Arsenic Speciation Analysis In Food Using Hplc/Icp-Ms: Method Development And Validation, Yu-Cheng Lai, Yi-Chen Tsai, Yu-Ning Shin, Ya-Chun Chou, Ying-Ru Shen, Nu-Ching Lin, Shu-Han Chang, Ya-Min Kao, Su-Hsiang Tseng, Der-Yuan Wang

Journal of Food and Drug Analysis

Arsenic (As) compounds can be classified as organic or inorganic, with inorganic arsenic (iAs) having significantly higher toxicity than organic As. As may accumulate in food materials that have been exposed to As-contaminated environments. Thus, the “Sanitation Standard for Contaminants and Toxins in Foods” published by the Ministry of Health and Welfare set the standard limits for iAs content in rice, seaweed, seafood, and marine oils to safeguard public health. Therefore, a robust analytical method must be developed to selectively and quantitatively determine iAs content in rice, seaweed, seafood, and marine oils. Herein, we reported and verified the method of …

Optimization Of Quechers And High Performance Liquid Chromatography-Fluorescence Detection Conditions To Assess The Impact Of Preparation Procedures On Eu Priority Pahs In Coffee Samples And Their Pahs Consumption Risk, Ying-Chun Wan, Zwe-Lin Kong, Yu-Hsuan Chao, Chia-Fang Teng, Deng-Jye Yang

Journal of Food and Drug Analysis

The good performance conditions for determination of EU priority PAHs in coffee samples were established to evaluate the effects of roasting degree on the PAHs in coffee beans and the brewing methods on the PAHs transfer from coffee beans to their brews. The consumption risk of the PAHs in coffee products was also assessed. The PAHs levels of the roasted coffee beans were in the order: 923.65 ng/g (dark roast) > 132.20 ng/g (medium roast) > 69.28 ng/g (light roast). Compared with general brewing with the drip bag (PAHs content, 0.30–0.62 ng/mL in coffee brews), the coffee machine brewing (set at 4 …

Effect Of Sterilization Conditions On The Formation Of Furan And Its Derivatives In Canned Foods With Different Substrates, Wen-Xuan Tsao, Bing-Huei Chen, Tsai-Hua Kao

Journal of Food and Drug Analysis

This study explored the effects of sterilization conditions on the formation of furan and its 10 derivatives in canned foods with a sterilizing value (F0) at 4. The contents of furans were determined by SPME arrow-GC-MS/MS, along with the furan precursors analyzed for elucidating the possible mechanism of furan formation. Results revealed that the total furan contents rose substantially in canned meat paste, tomato mackerel, chicken puree, tomato paste, pineapple slice, pineapple juice and carrot juice following sterilization. However, the total furan content did not change significantly ( p > 0.05) in canned oily mackerel, but decreased significantly ( p < 0.05) in canned apple puree after sterilization. With the exception of apple puree and pineapple slice, all the other canned foods showed a higher total furan content under low-temperature-long-time condition than that under high-temperature-short-time condition. Following heating, only the furan level showed a large increase in chicken puree, meat paste and tomato mackerel, whereas in canned fruit- and vegetable-based foods, the contents of furan and furfural showed a pronounced increase. The levels of alkylated furans were higher in sterilized samples containing high level of amino acid, while that of oxygenated furans were higher in sterilized samples containing high level of reducing sugar.

Simple And Rapid Detection Of Ractopamine In Pork With Comparison Of Lspr And Lfia Sensors, Min Ji Lee, Sheik Aliya, Eun-Seon Lee, Tae-Joon Jeon, Mi-Hwa Oh, Yun Suk Huh

Journal of Food and Drug Analysis

This study developed a simple and rapid strategic technique to detect ractopamine (chemical growth-promoting agent) in pork. Two highly sensitive and specific gold nanoparticle-based portable sensors, i.e., localized surface plasmon resonance (LSPR) sensors, and lateral flow immunoassay (LFIA) strips were developed to detect veterinary drug residues in food products, that have detrimental effects on humans. Optimization studies were conducted on several sensor devices to improve sensitivity. Each sensor comprised functionalized gold nanoparticles conjugated with ractopamine antibodies. The LSPR sensor chip achieved excellent detection sensitivity = 1.19 fg/mL and was advantageous for quantitative analysis due to its wide dynamic range. On …

Recent Trends In Analysis Of Mycotoxins In Food Using Carbon-Based Nanomaterials, Bing-Huei Chen, Baskaran Stephen Inbaraj

Journal of Food and Drug Analysis

Mycotoxins (MYTs), a class of low molecular weight secondary metabolites produced by filamentous fungi in food and feed, pose serious global threat to both human health and world economy. Due to their mutagenic, teratogenic, carcinogenic and immunosuppressive effects, the International Agency for Research on Cancer has classified various MYTs under Group 1 to 3 category with aflatoxins being designated under Group 1 category (carcinogenic to humans). Also, the presence of MYTs in trace amounts in diverse food matrices necessitates exploration of highly sensitive methods for onsite analysis. Although conventional chromatographic methods are highly sensitive, they are expensive, tedious and cannot …

Recent Advances In Immunoassay-Based Mycotoxin Analysis And Toxicogenomic Technologies, Winnie-Pui-Pui Liew, Mohd-Redzwan Sabran

Journal of Food and Drug Analysis

The co-occurrence and accumulation of mycotoxin in food and feed constitutes a major issue to food safety, food security, and public health. Accurate and sensitive mycotoxins analysis can avoid toxin contamination as well as reduce food wastage caused by false positive results. This mini review focuses on the recent advance in detection methods for multiple mycotoxins, which mainly depends on immunoassay technologies. Advance immunoassay technologies integrated in mycotoxin analysis enable simultaneous detection of multiple mycotoxins and enhance the outcomes’ quality. It highlights toxicogenomic as novel approach for hazard assessment by utilizing computational methods to map molecular events and biological processes. …

Analysis Of Highly Polar Pesticides In Foods By Lc-Ms/Ms, Wan-Rou Liao, Kuan-Lu Wu, Kun-Hao Chiang, Chieh-En Teng, Sung-Fang Chen

Journal of Food and Drug Analysis

Highly polar pesticides (HPP) are a group of pesticides that are characterize as low Log Kow. Many high-throughput multi-residue analysis methods based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of such polar pesticides have been proposed. In this article, we summarize the various sample preparation methods including quick polar pesticides (QuPPe), dispersive solid phase extraction (dSPE), solid phase extraction (SPE) and QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe), especially for QuPPe, which are mainly used for the determination of HPP in foods. In addition, we summarize LC-based separation methodologies that are currently used for the analysis …

Recent Advances In The Analysis Of Polycyclic Aromatic Hydrocarbons In Food And Water, Bing-Huei Chen, Baskaran Stephen Inbaraj, Kuo-Chiang Hsu

Journal of Food and Drug Analysis

Polycyclic aromatic hydrocarbons (PAHs), a class of harmful and persistent organic contaminant, are widely distributed in the environment and eventually accumulated in water and food. Also, they are formed in different varieties and varying amounts during processing of food depending on the food composition, cooking method and processing condition. According to the International Agency for Research on Cancer (IARC), various PAHs are classified under Group 1 to 3 category, with Group 1 designated as carcinogenic to humans, Group 2A as probable carcinogen, Group 2B as possible carcinogen and Group 3 as noncarcinogenic. Therefore, it is imperative to develop rapid and …

Preface, Bing-Huei Chen

Journal of Food and Drug Analysis

No abstract provided.

Supporting The Protect Initiative, Josh Lefton, Jackson Murray, Ahmed Thabet, Sriram Baireddy, Prakash Shukla, Mridul Gupta, Reagan Becker, Julie Ertle, Tony Doan, Aerin Yang

Purdue Journal of Service-Learning and International Engagement

Recently, medication dosage errors have received more political and media attention. Dosage errors are the most common medical errors, affecting about 1.5 million people annually.

Furthermore, U.S. poison-control centers reported more than 200,000 cases per year of medication errors. These cases result in medical costs of around $3.5 billion, and children under 6 years old constitute approximately 30% of these cases.

The PROTECT Initiative (Preventing Overdoses and Treatment Errors in Children Taskforce) was launched in 2008 as a collaborative effort between public health agencies and patient advocates to minimize dosage errors.

In alignment with the PROTECT Initiative effort, this project …

Impact Of A Digital Educational Program On The Health Literacy And Perceived Drug Teratogenic Risk Of Diabetic Pregnant Women Attending A Malaysian Teaching Hospital, Sami Al-Shakhshir, Syed Azhar Sulaiman, Mohd Pazudin Ismail, Mahmoud S. Alhaddad, Yaser Mohammed Al-Worafi, Islam M. Ashram

The Thai Journal of Pharmaceutical Sciences

ABSTRACTObjectives: The study aimed to identify the impact of clinical pharmacist’s digital educational program on health literacy and drug teratogenicity risk among Malaysian diabetic pregnant women. Methods: A quasi-experimental study was undertaken at the antenatal clinic at Universiti Sains Malaysia Hospital (HUSM) in Malaysia. The study was carried out with 138 diabetic pregnant women who were conveniently interviewed to measure their health literacy and teratogenic risk perceptions before and after educational program which was conducted using WhatsApp application. Descriptive and inferential analyses were performed using SPSS version 22. P-values of less than 0.05 were considered significant. Results: Most participants had …

Citrus Aurantium Peels Secondary Metabolites Prevents Cell Senescence On Human Fibroblasts Cells By In Vitro And Bioinformatics Studies, Nur Dina Amalina, Sri Wahyuni, Harjito H, Meiny Suzery, Bambang Cahyono

The Thai Journal of Pharmaceutical Sciences

Aging is a medical problem affecting about 800 million people worldwide. However, the molecular mechanism of aging and aging-related disease has not yet been explored. Skin aging is a complex biological process primarily due to cellular senescence. Cellular senescence is an irreversible growth arrest that caused tissue to lose their cell-specific functionality, secreting pro-tumorigenic and pro-inflammatory cytokines, which eventually causes cancer. Citrus aurantium-derived secondary metabolites, including hesperidin, hesperetin, tangeretin, naringenin, nobiletin, and quercetin, are of vital importance to inhibiting aging due to their active properties. However, the effect of C. aurantium-derived secondary metabolites on the cells senescence and its molecular …

Synthesis, Docking Studies, Admet Prediction, And Antimicrobial Evaluation Of Coumarin-Oxadiazole Hybrids, Fabin Asmath Maziyuna, Abhishek Kumar, Pankaj Kumar

The Thai Journal of Pharmaceutical Sciences

The overuse and misuse of marketed antimicrobial agents developed drug resistance, which has become a significant problem worldwide. A series of new coumarinyl-oxadiazole Schiff base derivatives were designed and synthesized. These synthesized molecules synthesis of coumarinyl-oxadiazole schiff base derivatives (FP1-FP7) were characterized using Infrared spectroscopy, proton nuclear magnetic spectroscopy, and mass spectroscopy and evaluated for their antibacterial potential against Gram-negative (Escherichia coli, Pseudomonas aeruginosa) and Gram-positive (Bacillus subtilis, Staphylococcus aureus) bacterial strain by measuring minimum inhibitory concentration values. Among the synthesized compounds, FP6 was found to be the best antibacterial agent having concentration values of 3.12 and 6.25 μg/ml against …

The Synergistic Antitumor Effects Of Curcuma Longa And Phyllanthus Niruri Extracts On Promoting Apoptotic Pathways In Breast Cancer Stem Cells, Dedy Hermansyah, Delfitri Munir, Aznan Lelo, Agung Putra, Nur Dina Amalina, Iffan Alif

The Thai Journal of Pharmaceutical Sciences

Breast cancer stem cells (BCSCs) develop apoptosis resistance by expressing pro- and anti-apoptotic protein including caspase-3, p53, and survivin. However, the induction of apoptosis is an important mechanism of action for many anticancer agents. Therefore, treatment using combination therapy was supposed to inhibit apoptosis resistance and increase the possibility of cancer apoptosis. This study aimed to investigate the effects of the combination Curcuma longa (CL) and Phyllanthus niruri (PN) extract on apoptosis induction. The single and combination cytotoxicity was analyzed using MTT assay. Apoptosis was measured by Annexin V-Propidium Iodide under flow cytometry. Caspase 3 expression was assessed by flow …

Carbon Dots: An Updated Overview On Their Synthesis, Properties, And Applications In Pharmaceutical And Biomedical Sciences, Earle Radha Rani, Majji Usharani, A. Lakshmi Usha

The Thai Journal of Pharmaceutical Sciences

Nowadays, carbon-based materials have acquired more attention in numerous fields including engineering, medicine, and pharmaceutics. As a new class of small carbon nanomaterials, carbon dots are developed due to their excellent physicochemical properties such as spherical shape, non-toxicity, cost effectiveness, facile, biocompatibility, and discrete particles <10 nm in diameter. In 2004, these carbon dots were first discovered accidentally during processing of single walled carbon nanotubes. Carbon dots have created huge excitement due to their advantages such as high water solubility, excellent biocompatibility, and resistance to photo bleaching. Carbon dots show unique properties such as photo stability, low toxicity, fluorescence, photo chemiluminescence, and electrochemiluminescence. Due to these properties, they have a wide range of applications in the field of bioimaging and drug delivery. Carbon dots are classified into two groups: Amorphous based carbon dots and graphene-based quantum dots with sp2 hybridization. The synthesis methods of carbon dots were reviewed along with their advantages and disadvantages. The applications of carbon dots in drug delivery, gene delivery, targeted drug delivery, crossing blood–brain barrier, and bio imaging were discussed.

Insights Into Novel Therapeutic Targets And Molecular Aspects In Polycystic Ovarian Syndrome: A Review, Rukaiah Fatma Begum, M. Sumithra, V. Chitra, Sharmila Devi

The Thai Journal of Pharmaceutical Sciences

Polycystic ovarian syndrome (PCOS) is a common endocrine syndrome in women associated with many other complications such as insulin resistance, obesity, hirsutism, increased risk of cardiovascular diseases, and infertility. As the pathophysiology of the syndrome still is not clear, it is difficult for the health-care professionals to precisely treat the syndrome. The cause of this syndrome is also unknown, but the evidences suggest that the factors such as environment, diet, and lifestyle are also a very common cause now a day for the syndrome. Recent pathophysiology of PCOS based on the Anti-Mullerian Hormone (AMH) and melatonin levels has been seen …

Efficacy Of Investigative Candidate Drugs Against Sars Cov-2: An Update On Pre-Clinical And Clinical Trials, Maryam Bibi, Aatika Sadia, Muhammad Shahzad, Ahmad Irfan, Muhammad Asim Raza Basra

The Thai Journal of Pharmaceutical Sciences

The unprecedented challenges presented by COVID-19 pandemic demands for an urgent development of effective therapeutic strategies against the deadly virus. Researchers thus far have revealed outcomes of pre-clinical and clinical studies of different interventions. This study aims to present overall summary of therapeutics that until this date are reported for their efficacy against COVID-19 in pre-clinical and clinical trials. A thorough literature search was conducted using databases for identification of studies published on the severe acute respiratory syndrome CoV (SARS COV-2) treatment. According to pre-clinical experiments, Nefamostat mesylate, Remdesivir, Chloroquine (CQ), Hydroxychloroquine (HCQ), β-D-N4-hydroxycytidine (NHC EIDD-1931), Ivermectin, Boceprevir, Calpain inhibitors, …

Assessment Of Risk Factors And Prescription Analysis Among Embolic And Atherosclerotic Stroke Patients In A Charitable Hospital-A Retrospective Study, Shanty Mary Jose, Amala Johnson, Akshay Abraham, Uday Venkat Mateti, Adithi Kellarai, Raushan Kumar Chaudhary, Shraddha Shetty

The Thai Journal of Pharmaceutical Sciences

Purpose: Despite the burden of stroke globally, there is a lack of data on the current clinical profile of stroke in middle and low-income countries including, India. Hence, the purpose of this study was to identify risk factors, clinical presentations, and importantly, prescription trends in ischemic stroke patients. Methods: A retrospective study was conducted for six months using data of 350 ischemic stroke patients for the analysis of risk factors and prescription pattern. Results: The incidence of ischemic stroke was higher (66%) in males in compared to (34%) females with mean age of 61.07 ± 11.460. Further the atherosclerotic stroke …

Hepatotoxic And Nephrotoxic Effects Of Chronic Exposure To Vernonia Cinerea Crude Extract On Wistar Rats, Thularath Toommakorn, Sajee Waramit, Patchara Danaisawadi, Kantimanee Pradermwong

The Thai Journal of Pharmaceutical Sciences

Vernonia cinerea L. (VC) has been used for smoking cessation and decreased appetite in Thailand. However, the safety of this plant concerning long-term consumption has never been reported. A 6-month toxicity study of VC water crude extract (VCWE) was performed in both genders of Wistar rats at doses of 15, 150, and 1500 mg/kg body weight/day orally. Recovery group received 1500 mg/kg for 6 months and then extended for 14 days without the extract. It was found that the males could not tolerate the highest dose of VCWE. There was no effect on bodyweight, relative organ weight, nor hematological parameters …

Anti-Diabetic Effect Of Interruptins A And B From Cyclosorus Terminans On Mouse Hepatocytes, Sujinda Songtrai, Sireewan Kaewsuwan

The Thai Journal of Pharmaceutical Sciences

Interruptin B from the fern Cyclosorus terminans has been indicated as a ligand of peroxisome proliferator-activated receptor (PPAR)-α and -γ that is known to regulate glucose homeostasis. Interruptin B was previously been implicated to promote glucose consumption into adipocytes. This study aimed to examine the anti-diabetic impact of interruptins A and B on glucose uptake and glycogen synthesis in mouse hepatocytes. Cell viability of FL83B hepatocytes affected by interruptins A and B was determined. The glucose consumption after 12–24 h treatment with compounds was evaluated by glucose oxidase method. The glycogen content was assessed after 24 h incubation using anthrone …

Alcl3 Induced Neuroinflammation And Excitotoxicity In Brain Striatum Mediated Memory And Motor Dysfunction Ameliorated Via Scopoletin Enriched Canscora Decussata Extract, A. Roja, T. Ambiga, Abdul Khayum, Sulaiman Mohammed, Venkatesh Gunasekaran

The Thai Journal of Pharmaceutical Sciences

Objective: Scopoletin is one of the dynamic and active constituents originate in Canscora decussata (CD) herb from Shankhpushpi family according to Indian system of medicine. This study aimed to characterize and to explore the role of scopoletin enriched CD (SECD) extract on AlCl3-induced neuroinflammation and excitotoxicity in rat model. Methodology & Results: High-performance thin-layer chromatography finger printing of ethanol extract of CD confirmed that enriched scopoletin presence as its main constituent. AlCl3 induced behavioural changes were reversed after SECD (200 and 400 mg/kg, i. p) and Rivastigmine (0.3 mg/kg, i.p) treatment by the way of Y maze, Rota rod, and …

Anti-Inflammatory Activity Of Phenolic-Rich Fraction Of Pandanus Tectorius Fruits Via Reduction Of No Production On Lipopolysaccharide-Stimulated Raw264.7 Cell Lines And Formalin-Induced Paw Oedema, Yosie Andriani, Nadia Madihah Ramli, Noor Suryani Musa, Kanwal Kanwal, Habsah Mohamad, Hermansyah Amir, Murni Nur Islamiah Kassim, Desy Fitrya Syamsumir, Tengku Sifzizul Tengku Muhammad, Suvik Assaw

The Thai Journal of Pharmaceutical Sciences

Background: The current study is the first study that investigated the potential of phenolic-rich fraction of Pandanus tectorius fruits as an anti-inflammatory agent. The fruit is rich in phenolic compounds, especially from the flavonoids group. Phenolics are known to contribute to an anti-inflammatory activity. Objectives: The first objective of the study was to evaluate the anti-inflammatory potency of phenolic-rich fractions of P. tectorius fruits by in vitro and in vivo studies. The second was to investigate the cytotoxicity against RAW264.7 cell lines, antioxidant, and phenolic contents to support the experiment. Materials and Methods: Sample fractionation was done with hexane, ethyl …

Antioxidant And Nephroprotective Effects Of Delphinium Denudatum Root Extract (Medicinal Plant) Against Gentamicin-Induced Nephrotoxicity, Nadeem Ahmad Siddique

The Thai Journal of Pharmaceutical Sciences

Introduction and Objectives: Kidney is a front line organ exposed to the toxicity of gentamicin. The objective of this study was to determine the biopotential of Delphinium denudatum Wall., root a significant medicinal plant, against gentamicin. Materials and Methods: By performing high-performance liquid chromatography, an in vitro antioxidant study was conducted against diphenylpicrylhydrazide (DPPH). In vivo nephroprotective activity was evaluated by administering solo administration of gentamicin (GM; 40 mg/kg b.wt. single dose s.c.) for a period of 1st–13th days followed by hydroalcoholic extract of D. denudatum (HAEDD; 200, 400, and 800 mg/kg b.wt. intragastrically) to the rats for 14th–23rd days. …

Antidiabetic Study And Teratogenicity Evaluation Of Ethanolic Extract Of Chromolaena Odorata Linn., Indah Solihah, Yuni Fitriani, Meutia Reni Noprida, Herlina, Riana Sari Puspita Rasyid, Tri Suciati

The Thai Journal of Pharmaceutical Sciences

There is considerable interest in herbal medicine in developing antidiabetic alternative therapy based on plant-derived compounds that would reduce oral hypoglycemic agent-related adverse effects while staying safe during pregnancy. The purpose of this in vivo study was to evaluate the ethanolic extract of Chromolaena odorata L. leaves (ECOL) in alloxan-induced diabetes and its teratogenic effect. The hypoglycemic impact of ECOL increased when the extract dose was raised. Three doses of ECOL (125, 250, and 500 mg/kg body weight) showed a 27.29%, 34.68%, and 52.24% hypoglycemic effect, respectively. While, the standard insulin (1 IU/kg BW) group shown a 31.38% hypoglycemic effect …