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Evaluation Of A Novel Rapid Diagnostic Test For Schistosoma Haematobium Based On The Detection Of Human Immunoglobulins Bound To Filtered Schistosoma Haematobium Eggs, Johnathan M. Sheele, Jimmy H. Kihara, Sarah Baddorf, Jonathan Byrne, Bhaskara Ravi
Evaluation Of A Novel Rapid Diagnostic Test For Schistosoma Haematobium Based On The Detection Of Human Immunoglobulins Bound To Filtered Schistosoma Haematobium Eggs, Johnathan M. Sheele, Jimmy H. Kihara, Sarah Baddorf, Jonathan Byrne, Bhaskara Ravi
Mathematics & Statistics Faculty Publications
Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh).
Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S.haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S.haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.
Results The RDT-Sh was positive in …