Other Life Sciences Commons^™

The Effect Of Wee1 On Mitosis In The Spb Mutant, David Leroy

Honors Theses

The speedbump mutant has been identified as an early arrest mutant, becoming apparent as soon as 80% epiboly (8.5 hours of development). This mutant has a mutation in wee1, which is a protein regulating entry into mitosis ensuring the completion of DNA replication before mitosis. The lack of this protein in speedbump allows for early entry into mitosis resulting in damage to the chromosome. Here, we investigate two questions: if mitosis in the speedbump mutant phenotype is more frequent than wild-type phenotype, and, if so, is there a phase in which the mutant phenotype favors. We used phosphorylated histone H3 …

The Impact Of Stability Ball Seating On Behavior And Academic Achievement Among Elementary School Students In Grades 1 Through 4., Sheila R. Borders

Doctoral Dissertations

This study was designed to answer three questions: 1) What is the impact of stability ball seating on the behavior of students in grades 1 through 4? 2) What is the impact of stability ball seating on the mathematics scores of students in grades 1 through 4? and 3) What is the impact of stability ball seating on the reading scores of students in grades 1 through 4? Research has shown that stability ball seating is linked to behavior and academic achievement among students diagnosed with attention deficit hyperactivity disorder (ADHD) and/or autism spectrum disorder (ASD). This study examines the …

Intercanine Distance Used As A Measure To Rule Out Biters In Bite Mark Forensics, Alana Joy Scudiere Ohana

Masters Theses

The science of identification by bite mark analysis has recently been called into serious question. (Reesu and Brown 2016) Human dentition is truly variable, but often not unique. When animal bites are considered, a proper ID of the perpetrator is nearly impossible. Primary distortion (when the bite is made) and secondary distortion (during decomposition or healing) both further disrupt landmarks that might be used for identification. (Sheasby and MacDonald 2001) Using only ink marks and intercanine distance on live subjects, this study attempts to determine maximum distortion possible for a variety of bite mark locations on skin. (Pretty and Sweet …

Species Pluralism: Conceptual, Ontological, And Practical Dimensions, Justin Bzovy

Electronic Thesis and Dissertation Repository

Species are central to biology, but there is currently no agreement on what the adequate species concept should be, and many have adopted a pluralist stance: different species concepts will be required for different purposes. This thesis is a multidimensional analysis of species pluralism. First I explicate how pluralism differs monism and relativism. I then consider the history of species pluralism. I argue that we must re-frame the species problem, and that re-evaluating Aristotle's role in the histories of systematics can shed light on pluralism. Next I consider different forms of pluralism: evolutionary and extra-evolutionary species pluralism, which differ in …

Ecological Consequences Of Lost Anadromous Forage Fish In Freshwater Ecosystems, Steven R. Mattocks

Masters Theses

Beginning in the early 1600s, dam construction in New England obstructed anadromous fish access to spawning grounds during migration. As a result, anadromous forage fish populations have declined, which has impacted freshwater, marine, and terrestrial ecosystems. To determine the impacts of dams on anadromous forage fish and freshwater ecosystems, I used historical and current data to estimate population changes in alewives (Alosa pseudoharengus) from 1600-1900. A significant reduction in spawning habitat occurred in New England as a result of 1,642 dams constructed between 1600 and 1900, resulting in 14.8% and 16.6% lake and stream habitat remaining by 1900, …

An Investigation Of The Succination Of The Selenoproteins Thioredoxin Reductase And Glutathione Peroxidase, Scott Lanci

Theses and Dissertations

We have identified the irreversible protein modification S-(2-succino)cysteine, a product of the Kreb’s cycle intermediate fumarate reacting with the thiol group of cysteine (Cys) residues, also termed succination. Succination is increased in adipose tissue of diabetic mice in vivo and in adipocytes matured in high glucose medium (25 mM) in vitro. Selenocysteine (Sec) is a less common but highly conserved amino acid that has major functions in redox chemistry. Sec possesses a lower pKa (~5.4) and is much more reactive in comparison to Cys. This project aimed to determine if succination occurred on Sec residues in two selenoproteins that play …

Tumor Suppressor P53 Response To Uv Light In Normal Human Keratinocyte Strains From Different Individuals, Fadi Farooq Abboodi

Theses and Dissertations

Human Papillomavirus (HPV) is a causative factor in variety of diseases ranging from simple pathologic conditions to malignant ones like cervical cancer in which HPV is the primary cause in more than 90% of cases. HPV is also associated with other tumors like head and neck squamous cell carcinoma (HNSCC) and the percentage of association varies according to the anatomical site, reaching its highest level (65%) in the oropharyngeal region. HPV proteins E6 and E7 are responsible for the transforming potential of the virus. The principal action of E6 is targeting the tumor suppressor protein p53 for degradation, while E7 …

Investigation Of Succinated Protein Turnover By Autophagy, Lydia Proctor

Theses and Dissertations

Background. Diabetes is a widespread public health concern that alters the metabolism of adipocytes through high glucose stress and hormonal dysregulation. Under diabetic conditions there are excess nutrients available and subsequently an accumulation of tricarboxylic acid cycle intermediates such as fumarate. Fumarate can irreversibly react with protein cysteine residues to form S-2-succinocysteine (2SC, protein succination). Succinated proteins can be turned over by autophagy, a mechanism of removing damaged proteins and organelles that have accumulated due to metabolic stress.

Purpose. The basal flux through autophagy in adipocytes matured in non-diabetic and diabetic conditions was examined to determine the turnover of succinated …

Changes In Floristic Composition In The State Forests In Worcester County (Massachusetts) Over 34 Years, Flor A. Monroe

International Development, Community and Environment (IDCE)

ABSTRACT

CHANGES IN FOREST DIVERSITY OF STATE FOREST IN WORCESTER COUNTY, MASSACHUSETTS, OVER 34 YEARS PERIOD

The forest in Massachusetts has changed since the earliest colonial settlement and today the floristic composition is more homogeneous. This study investigates the potential change in the floristic composition over thirty years in Worcester County State Forests. Shannon, richness and evenness indices for two periods were compared, and Jaccard index was used to analyze similarity in composition between the periods. The possible influence of severe weather events was also analyzed.

It was found Changes in the floristic composition, but the magnitude of the changes …

Development Of A Decision Support System For Post Mining Land Use On Abandoned Surface Coal Mines In Appalachia, Matthew Zimmerman

International Development, Community and Environment (IDCE)

Decision support systems are diverse and have been used to solve multiple problems ranging from the complex to the simple. With the complexity of environmental decisions today, these systems provide a logic based approach to evaluating and choosing environmental solutions. Abandoned mining lands (AML) are an issue for the environment in the Appalachian region. Given this a decision support system was designed using previously created frameworks and indices from other systems created. The system is comprised of two main sections, selecting the ideal post-mining land-use (PMLU), and maximizing the potential of land to be reclaimed under budgetary constraints. This system …

Discharge-Suspended Sediment Relations: Near-Channel Environment Controls Shape And Steepness, Land Use Controls Median And Low Flow Conditions, Angus A. Vaughan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Erosion, transport and deposition of fine sediment (clay, silt and fine sand) influence the form and function of river systems. Excess suspended sediment degrades stream ecosystems and is implicated as a leading cause of water quality and aquatic life impairment. Therefore, understanding the factors that control fine sediment transport patterns is an interesting topic for basic science and one that has important management and policy implications.

In this study, we sought to understand how attributes of the landscape and channel network might control the shape, steepness and vertical offset of the relationship between river discharge and suspended sediment. Watershed and …

Effects Of Environmental Water Rights Purchases On Dissolved Oxygen, Stream Temperature, And Fish Habitat, Nathaniel R. Mouzon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Degraded water quality has reduced aquatic species abundance and survivability in Nevada’s Walker River. Low instream flows and increased nutrients affect native fish populations through high daily stream temperatures and low nightly dissolved oxygen concentrations. Increasing streamflow, through environmental water purchases, may improve water quality and enhance habitat for native fish species, such as Lahontan cutthroat trout. This study uses River Modeling System, a computer model, to estimate streamflows, stream temperatures, and dissolved oxygen concentrations in the Walker River. Streamflow increases are simulated to determine potential improvements to high water temperatures and low dissolved oxygen concentrations, enabling the prioritization of …

Bacteriophages That Can Lyse Antibiotic Resistant Bacteria, Michael Monaco

Honors Theses

A Klebsiella pneumoniae bacteriophage was isolated from waste water and purified over multiple rounds of plaque removals and serial titrations. This bacteriophage was imaged in a transmission electron microscope and then tested with selected antibiotics for effectiveness in reducing optical density of K. pneumoniae colonies. The phage was shown to have an increased effectiveness in reducing the optical density when used together with an antibiotic that the bacteria was susceptible to.

"Patterns In The Abundance Of Benthic Algae In Streams", Courtney Wright

Honors Theses

Benthic, microscopic algae are the main drivers of primary productivity in streams. The relationship between the abundance and productivity of benthic algae in streams and lakes and the availability of limiting nutrients, especially soluble reactive phosphorus (SRP), has been extensively studied (reviewed in Allan and Castillo 2007, Larned 2010). However, nearly all of this research has been conducted on the periphyton assemblage found on hard substrates (e.g., stones, wood), while very little work has addressed the factors affecting the abundance of epipelic algae (i.e., the algae associated with depositional (soft) sediments such as sands and silts). One of the few …

Changes In Wetland Conditions And Wetland Plant Communities In The Prairie Pothole Region After 50 Years, Ryann Cressey

Electronic Theses and Dissertations

Wetlands in Stutsman County, North Dakota were revisited after 50 years to assess changes in wetland conditions and plant communities within wetland zones in the Prairie Pothole Region. In 1961-1966, Robert E. Stewart and Harold A. Kantrud conducted a study to investigate the relationship of wetland plant communities to variations of water permanence and chemistry on three study areas: Crystal Springs, Cottonwood, and Mt. Moriah. Within in this region, a severe drought occurred in 1988- 1992 followed by the longest deluge starting in 1993 with wetlands still impacted today. In 2013 and 2014, I revisited 80 of the original wetlands …

Mislabeling Of Commercial Atlantic Cod (Gadus Morhua) Products In Spain, Joshua Helgoe

Theses and Dissertations

The mislabeling of commercial fish products is a pervasive, worldwide problem. Most consumers of seafood are unaware this issue directly affects them and can even have negative impacts on their health. Mislabeling occurs when a product’s label is inconsistent with its content. Although mislabeling can be unintentional, deliberate mislabeling is a more common trend to increase profits and/or bypass fishing regulations - a form of economic fraud. Unfortunately, oversight, enforcement, and research are vastly insufficient in relation to the global scale of the problem. In order to add to the small knowledge base on European mislabeling rates, determine if overfished …