Other Nutrition Commons^™

Adipose Tissue Abca1 Contributes To Hdl Biogenesis In Vivo, Soonkyu Chung, Janet K. Sawyer, Abraham K. Gebre, Nobuyo Maeba, John S. Parks

Department of Nutrition and Health Sciences: Faculty Publications

Background—Adipose tissue (AT) is the body’s largest free cholesterol (FC) reservoir and abundantly expresses ATP binding cassette transporter A1 (ABCA1), a key cholesterol transporter for HDL biogenesis. However, the extent to which AT ABCA1 expression contributes to HDL biogenesis in vivo is unknown.

Methods and Results—Adipocyte-specific ABCA1 knockout mice (ABCA1^−A/−A) were

generated by crossing ABCA1floxed mice with aP2 cre transgenic mice. AT from ABCA1^−A/−A mice had −^A/−A mice had a twofold increase in FC content, compared to WT mice, and failed to efflux cholesterol to apoA-I. …