Nutrition Commons^™

Effects Of Statin Drugs And Tocotrienol Rich Fraction Supplementation In Chronic Hemodialysis Patients And Metabolomic Profile, Eno Latifi

Wayne State University Theses

Chronic kidney disease (CKD) is known as a heterogeneous disorder which currently is on the rise and lately has been classified as a public health issues in the United State and worldwide. CKD is an irreversible and progressive disease which can lead to kidney failure, and this is depicted by the advanced stage of the disorder when it reaches the point, that is classified as end stage of renal disease (ESRD) (Stage 5 of CKD) (eGRF <15 mL/min/ 1.73 m2 working capacity), where both organs are in a total or permanent kidney failure. End-Stage renal disease patients, on hemodialysis have been associated to experience an accelerated form of atherosclerosis, which is induced by inflammation, impairment of antioxidant system and elevated oxidative stress. Since the problem effecting ESRD patients is multifactorial, the objective of this investigation is to explore and look at the effects of supplementing with vitamin E-tocotrienol rich fraction (TRF), a micronutrient which has anti-inflammatory, antioxidant, and lipid lower capabilities into tackling these comorbid conditions experienced by this population. Therefore the aims of this investigation will be to explore changes in lipid profiles, inflammatory markers, and oxidative status, as well as look at any changes in metabolomic profiles. It was hypothesized that by supplementing with TRF a vitamin E, for 16 weeks in ESRD patients undergoing hemodialysis, it may help reverse and/or improve, oxidative status, inflammatory markers, increase antioxidants status and improve lipid profiles.

The study was double-blinded, randomized, parallel, placebo-controlled design trial, of 81 adult patients undergoing chronic hemodialysis at Great Lake Dialysis Clinic, Detroit MI, where …

Comparitive Study On Antioxidant Properties Of Dendrobium Officinale (Tiepishihu), Wenjun Zhu

Wayne State University Theses

For ages, Chinese herbs, such as Dendrobium Officinale (DO), have been used in Asian regions for treating various illnesses due to the high medicinal value. In this study, the DO plant Acetone and Ethanolic extracts possibly containing antioxidants were tested using different methodologies for comparison and determination of specific antioxidant capacity. The total phenolic content assay expressed as Gallic Acid Equivalent showed 9.43±;0.23 mg GAE /g of extract. Further analysis using DPPH· assay indicated that antioxidants presented in the sample were able to significantly scavenge 37% and 44% radicals at the concentrations of 25mg/ml and 50mg/mL respectively, after a period …

Effect Of Long Term Rapamycin Treatment On Mtor Signalling Network In Colon And Liver Of C57bl/6 Mice, John Sorge

Wayne State University Theses

Many studies have investigated the effects of rapamycin on aging and cancer. However, the effects of long-term rapamycin supplementation on a cancer model have not been performed. This is the first study that investigates the effects of long-term supplementation of rapamycin in a cancer model. ACF analysis of colon tissues in mice showed no significant difference between controls and those supplemented with rapamycin. Factors such as energy balance, cellular environment, PI3K/Akt/mTOR pathway, and more have been assessed in this study. The duration of rapamycin supplementation seems to play an important role in the protection against cancer. Ultimately, this study suggests …

The Impact Of Down Syndrome And Folate Depletion On Genomic Stabilizing Pathways Of Lymphoblastoid Cells, Khadijah Ibrahim Alnabbat

Wayne State University Theses

Understanding the role of nutrition plays in Down syndrome (DS) could help in the development and implementation of strategies that help overcome the negative consequences phenotypes of Down syndrome. Conserving genome integrity is crucial for cells to survive, and thus understanding how genetic defects induce damage to genomic DNA and impair subsequent repair of this damage is important. Evidence accumulated points to increased DNA damage and mutation accumulation associated with a decline in DNA repair capacity, Base Excision Repair (BER) in particular. Thus, the successful clinical management of DS resides in understanding the metabolic imbalance provoked by overexpression of genes …

Compare Effects Of Diets Made With Different Saturated Fatty Acids On Body Weight Regulation And Metabolism In Wister Rats, Dilruba Fatema

Wayne State University Theses

Animal fats are known to cause elevated blood total and low-density- lipoprotein (LDL) cholesterol levels in humans and animals. This study was designed to investigate the effects of both the quality (coconut oil and a modified beef tallow with reduced saturated fatty acid content) and quantity (low and high) of dietary fat in body weight regulation and alteration of blood cholesterol levels in an animal model. Forty two male Wister rats were randomly divided into 4 groups: Control Low Fat (CLF, n=10), Control High Fat (CHF, n=10), Modified Low Fat (MLF, n=11), and Modified High Fat (MLF, n=11). They were …

Comparative Phenolic Content And Antioxidant Activities Of Blueberry (Vaccinium Corymbosum) Afected By In Vitro Digestion, Sonia Gharbi

Wayne State University Theses

Abstract

COMPARATIVE PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITIES OF BLUEBERRY (VACCINIUM CORYMBOSUM) AFECTED BY IN VITRO DIGESTION

By

SONIA GHARBI

May 2014

Adviser: Dr. Kevin Zhou

Major: Nutrition and Food Science

Degree: Master of Science

The biological properties of antioxidants depend on their release from the food matrix during the digestion process. Blueberry contains a wide range of phenolic compounds which are of great significance due to their antioxidant activity. In vitro digestion is a rapid and inexpensive method used to determine the availability of nutrients involved in the absorption studies with humans. Total phenolic content and antioxidant activity of Vaccinium …

Saudi Arabian Students In Detroit Use Natural Health Products More So Than Other University Students In Detroit, Samiah Naji Alqahtani

Wayne State University Theses

Natural Health Products (NHP), are defined as products that derive from organisms and may include supplements and herbal/traditional remedies. Prior research indicates that females use NHP more than males. We have unpublished data to show that students use NHP for health maintenance with significantly different use of NHP by Native/Aboriginal students vs. other students. Less is known about Saudi students. We hypothesize that: (1) There are gender differences in NHP used; and (2) There are geo-ethnic differences in NHP used. We also determine the predictor variables that influence NHP use by students. Students from Wayne State University (n=560; 193 …

Effect Of Folate Deficiency On Mtor Signaling Network On The Liver Of Wild Type Mice, Essra Moussawi

Wayne State University Theses

Through various studies of dietary nutrients on the affects of the physiological and molecular pathways are a key study in understanding the interaction between dietary nutrients and the human genome that may severely impact the development of various cancers. A primary approach is observing the dietary nutrients, that we consume and how it plays a role in the cellular pathway of our bodies through experimental methods. Folate deficiency (FD) has shown, through studies, to play a role anti-cancer progression. Our goal was to observe the autophagy related proteins in the mTOR signaling pathway as it was suggested that mTOR may …

Effect Of Folate Deficiency On Expression Of Proteins On The Mtor Signaling Pathway In The Brain Of C57/Bl6 Mice, Nikita Patel

Wayne State University Theses

ABSTRACT

EFFECT OF FOLATE DEFICIENCY ON EXPRESSION OF PROTEINS ON THE mTOR SIGNALING PATHWAY IN THE BRAIN OF C57/BL6 MICE

by

NIKITA PATEL

December 2014

Advisor: Dr. Ahmad Heydari

Major: Nutrition and Food Science

Degree: Master of Science

Nutrient-gene interactions can significantly impact several cellular processes in the human body by altering important molecular pathways. Nutrition can affect brain structure, function and development throughout the life cycle; it can have a profound effect on cognition ad mental health leading to neurodegenerative diseases. It is important to elucidate the mechanisms by which diet can effect signaling pathways in the brain. The …