Nutrition Commons^™

Influence Of Native And Processed Cereal Grain Fibers On Gut Health, Junyi Yang

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Cereal fibers that can be metabolized by gut microbiota have been shown to promote the growth of beneficial bacteria in the gut. Increased consumption of cereal fibers may improve host / gut microbiota interactions in obesity and other metabolic diseases by normalizing gut dysbiosis. The present dissertation describes four research projects to assess the impact of cereal dietary fibers on gut microbiota and host metabolism. In the first study, we determined the treatment temperatures for production of soluble, non-digestible, feruloylated oligo- and polysaccharides (FOPS) from maize bran and wheat bran, and determined the fermentation properties of partially purified FOPS from …

Predictors Of Preschool Children's Body Mass Index: Breastfeeding Duration, Child Eating Behaviors, And Parental Feeding Practices, Amy J. Encinger

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The current study examined how breastfeeding duration, child eating behaviors (Satiety and Food Responsiveness), and parental feeding practices (Environment and Parental Control over Food) are related to body mass index (BMI) among preschool-aged children. Participants were 224 primary caregivers (M age = 30.36; 90% mothers) of preschool-aged children (M age = 4.22 years, 55% male) from three Midwestern preschools serving low-income children. Mediated multiple regression analyses examined the effects of the various child eating behaviors and parental feeding practices as mediators of the relation between breastfeeding duration and child BMI. Three main findings emerged. There was a significant …

Discovery Neighborhood Myplate Macy: A Pilot Study, Amanda M. Robine

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Background: Obesity prevalence in United States children ages 2-19 years has been consistently high since 2003.1 These children are at a greater risk for many chronic disease. Educating children on how to make healthful food choices, and using fun characters in a classroom setting may be one step to reducing the obesity rate.

Objective: Increase knowledge about MyPlate and heathy eating in children in kindergarten through fifth grade.

Design: After the development of a nutrition lesson, an expert panel reviewed and suggested changes. Once changes were made the lesson was delivered to second and third graders at a summer camp. …

Characterizing The Effects Of Both Synthetic And Natural Inhibitors On The Function Of Holocarboxylase Synthetase And Lipid Metabolism, Elizabeth Cordonier

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The central theme of this dissertation explores enzyme inhibition, through the use of both synthetic and natural occurring inhibitors of three biotin-associated enzymes; the mammalian biotin ligase, holocarboxylase synthetase(HLCS), and acetyl-CoA carboxylases 1 and 2 (ACC1 and 2), whose activity is dependent on biotinylation by HLCS, and are involved in fatty acid synthesis and regulation of beta-oxidation, respectively. No null HLCS individual has ever been reported and individuals with inherited mutations in the HLCS gene, which render it incapable of biotinylating carboxylases, exhibit varying degrees of symptoms associated with multiple carboxylase deficiency. Little is known with regard to the reaction …

Attenuation Of Mtorc1-Driven Secretion Of Lipoproteins And Triacylglycerides By Short Chain Fatty Acids: Mechanistic Insight Into The Pathogenesis Of Hypertriglyceridemia, Joseph L. Roberts

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The mechanistic target of rapamycin complex 1 (mTORC1) is a serine/threonine kinase that drives several anabolic processes including lipid synthesis, protein synthesis, and adipogenesis. mTORC1 is highly active in the livers of obese rodents, in overnutrition, and is implicated in the development of obesity related metabolic disorders, including the overproduction of atherogenic lipoproteins. Direct inhibition of mTORC1 is not a viable treatment strategy because it prevents feedback inhibition of the insulin-signaling cascade, leading to increased lipid synthesis and secretion of lipoproteins. Thus, therapeutic approaches that drive catabolic pathways are considered promising mechanisms for overcoming mTORC1-driven anabolism. Human liver HepG2 cells …

Food Resource Management Education With Snap Participation Improves Food Security, Lucia Kaiser, Virginia Chaidez, Susan Algert, Marcel Horowitz, Anna Martin, Concepcion Mendoza, Marisa Neelon, David C. Ginsburg

Department of Nutrition and Health Sciences: Faculty Publications

Objective: To determine the influence of Supplemental Nutrition Assistance Program (SNAP) and participant demographics on nutrition education outcomes.

Methods: At program enrollment (pre) and 1 month later (post), a statewide convenience sample of adults, who participated in the Plan, Shop, Save, and Cook program, completed a 7-item questionnaire to evaluate change in resource management skills (RMS) and running out of food before the end of the month.

Results: Percent of participants (n = 3,744) who reported behavioral improvements in RMS ranged from 38.8%in comparing prices to 54% in reading labels. Female gender and Hispanic ethnicity were positively related to pre–post …

Micrornas Are Absorbed In Biologically Meaningful Amounts From Nutritionally Relevant Doses Of Cow’S Milk And Chicken Eggs And Affect Gene Expression In Peripheral Blood Mononuclear Cells, Cell Cultures, And Mouse Livers, Scott Baier

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Throughout the twenty-first century, evidence has been continually increasing to show the importance of epigenetic regulation in health. While the term “epigenetics” can be applied to many different processes, the focus of this dissertation will be on microRNAs and chromatin structure. Ultimately, both of these forms of epigenetic regulation can be used to fine tune gene expression based on environmental cues. The first three chapters of the dissertation focus on microRNA bioavailability, stability, and function from two commonly consumed food products: cow’s milk and chicken eggs. This important work has been the first of its kind to demonstrate the bioavailability …

Stability And Biological Activity Of Dietary Micrornas, Katherine Howard

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

MicroRNAs play important roles in gene regulation by binding to complimentary sites at the 3’ untranslated region of target mRNA molecules. Binding results in inhibition or degradation of target mRNA. Many bovine and chicken microRNA are homologous with human counterparts enabling gene regulation. A recent study in our lab provided undisputable evidence that endogenous milk microRNAs are bioavailable in humans; resulting in regulation of human gene expression. Based on these findings, we wanted to explore the possibility that other exogenous food borne microRNAs are able to be absorbed through the diet. My thesis surrounds two aims: 1) assessing the stability …

Development And Validation Of A Social Cognitive Theory-Based Survey For Elementary Nutrition Education Program, Elisha Hall, Weiwen Chai, Wanda Koszewski, Julie A. Albrecht

Department of Nutrition and Health Sciences: Faculty Publications

Background: The Social Cognitive Theory (SCT) is a widely used model for developing elementary nutrition education programs; however, few instruments are available to assess the impact of such programs on the main constructs of the SCT. The purposes of this study were: 1) to develop and validate a SCT-based survey instrument that focuses on knowledge, behavior, and self-efficacy for fifth grade students; 2) to assess the relationships between knowledge, behavior, and self-efficacy; and 3) to assess knowledge, behavior, and self-efficacy for healthy eating among the fifth grade students.

Methods: A 40-item instrument was developed and validated using content validity …

Physiological Responses Underlying The Perception Of Effort During Moderate And Heavy Intensity Cycle Ergometry, Kristen C. Cochrane, Terry J. Housh, Ethan C. Hill, Cory M. Smith, Nathaniel D.M. Jenkins, Joel T. Cramer, Glen O. Johnson, Richard J. Schmidt

Department of Nutrition and Health Sciences: Faculty Publications

This study examined patterns of responses for physiological and perceptual variables during cycle ergometry at a constant rate of perceived exertion (RPE) within the moderate and heavy exercise intensity domains. Nineteen (mean age 21.3 ± 0.5 years; 43.4 ± 2.0 mL·kg⁻¹·min⁻¹ VO_2Peak) moderately trained cyclists performed an incremental test to exhaustion and two 60 min constant RPE rides at the RPE corresponding to the gas exchange threshold (RPE_GET) and 15% above the GET (RPE_GET+15%). Oxygen consumption (VO₂), respiratory exchange ratio (RER), heart rate (HR), minute ventilation (V_E), …

Cloning And Expression Of Ebi3 And P28 Subunits Of Human Interleukin 27 In E. Coli, Ameneh Koochaki, Mojgan Bandehpour, Sam Massahi, Mahrou Sadri, Bahram Kazemi

Department of Nutrition and Health Sciences: Faculty Publications

Background: Interleukin (IL)-27 is a heterodimeric cytokine belonging to IL-12 and IL-23 families, secreted by antigen presenting cells(APCs). The IL-27 is composed of 2 subunits: Epstein-Barr virus induced gene 3 (EBI3) and p28. IL-27 is an anti-inflammatory cytokine which has an inhibitory effect on Th17 population and suppress the IL-17 expression. It is suggested that IL-27 could be a potent drug candidate for treating auto immune diseases.

Materials and Methods: The EBI3 and p28 subunits of human interleukin 27 were constructed into plasmid vectors; they sub-cloned into pETDuet-1 expression vector in restriction sites of BamHI, SacI and NotI. Subsequently the …

Fruit And Vegetable Perceptions Among Caregivers Of American Indian Toddlers And Community Stakeholders: A Qualitative Study, Rachel C. Sinley, Julie A. Albrecht

Department of Nutrition and Health Sciences: Faculty Publications

American Indians experience higher rates of obesity than any other ethnic group living in the USA. This disparity begins to develop in early childhood, and the excess weight carried by American Indian children contributes to health conditions that can affect their quality of life by the time they enter preschool. These children consume less than recommended amounts of fruits and vegetables, a dietary pattern that may be related to the development of obesity and other health conditions. This qualitative study explored the fruit and vegetable intake of American Indian toddlers through use of the information-motivation-behavioral skills (IMB) model. Focus groups …

Reductions In Urinary Collection Frequency For Assessment Of Reproductive Hormones Provide Physiologically Representative Exposure And Mean Concentrations When Compared With Daily Collection, Heather C.M. Allaway, Nancy J. Williams, Rebecca J. Mallinson, Karsten Koehler, Mary Jane De Souza

Department of Nutrition and Health Sciences: Faculty Publications

Objective — To determine if reducing the frequency of urinary sample collection from daily to 5, 3, or 2 days per week during a menstrual cycle or 28-day amenorrheic monitoring period provide accurate representations of the reproductive hormone metabolites estrone- 1-glucuronide (E1G) and pregnanediol glucuronide (PdG) exposure and mean concentrations.

Methods — Exercising women presenting with eumenorrhea or exercise-associated menstrual disturbances collected daily urine samples for the assessment of E1G and PdG concentrations. After enzyme immunoassay analysis of the daily samples, E1G and PdG data were systematically removed from each menstrual cycle or amenorrheic monitoring period to mimic three reduced …

Bmp7 Drives Human Adipogenic Stem Cells Into Metabolically Active Beige Adipocytes, Meshail Okla, Jung-Heun Ha, Ryan E. Temel, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Adult humans have a substantial amount of inducible-brown (or beige) fat, which is associated with increased energy expenditure and reduced weight gain via thermogenesis. Despite the identification of key regulators of beige adipogenesis, impacts of dietary factors on adaptive thermogenesis are largely unknown, partly due to a lack of validated human cell models. Bone morphogenetic protein 7 (BMP7) is known to promote brown adipogenesis in rodent and human progenitor cells. However, controversy still surrounds the cellular identity in BMP7-mediated transition of white to brown adipocytes. The aim of this study is to confirm BMP7-derived human adipocytes as a relevant in …

Nutrition-Related Policy And Environmental Strategies To Prevent Obesity In Rural Communities: A Systematic Review Of The Literature, 2002–2013, Larissa Calancie, Jennifer Leeman, Stephanie B. Jilcott Pitts, Laura Kettel Khan, Kelly R. Evenson, Michelle Schreiner, Carmen Byker, Clint Owens, Jared Mcguirt, Ellen Barnidge, Wesley Dean, Donna Johnson, Jane Kolodinsky, Emily Piltch, Courtney Pinard, Emilee Quinn, Lauren Whetstone, Alice Ammerman, Sheila Fleischhacker

Department of Nutrition and Health Sciences: Faculty Publications

Residents of rural communities in the United States are at higher risk for obesity than their urban and suburban counterparts. Policy and environmental-change strategies supporting healthier dietary intake can prevent obesity and promote health equity. Evidence in support of these strategies is based largely on urban and suburban studies; little is known about use of these strategies in rural communities. The purpose of this review was to synthesize available evidence on the adaptation, implementation, and effectiveness of policy and environmental obesity-prevention strategies in rural settings.

Effects Of Partners Together In Health (Path) Intervention On Physical Activity And Healthy Eating Behaviors: A Pilot Study, Bernice C. Yates, Joseph Norman, Jane Meza, Kaye L. Stanek Krogstrand, Susana Harrington, Scott Shurmur, Matthew Johnson, Karen Schumacher

Department of Nutrition and Health Sciences: Faculty Publications

Background—Despite proven efficacy of cardiac rehabilitation (CR) in helping patients initiate physical activity and healthy eating changes, less than 50% of CR participants maintain changes 6 months later.

Objective—The objective of this feasibility study was to test the Partners Together in Health (PaTH) Intervention versus usual care (UC) in improving physical activity and healthy eating behaviors in coronary artery bypass graft (CABG) surgery patients and spouses.

Methods—An experimental, two-group (n = 17 couples/group), repeated measures design was used. CABG patients in both groups participated in Phase II outpatient CR. Spouses in the PaTH group attended CR with the patient and …

Food Safety Knowledge, Attitudes, And Behaviors Of Native American Families With Young Children: A Mixed Methods Study, Kara Vlasin-Marty, Paula Ritter-Gooder, Julie A. Albrecht

Department of Nutrition and Health Sciences: Faculty Publications

Children are at increased risk for foodborne illness due to underdeveloped immune system. Limited research has been reported on food safety knowledge of Native American families with children 10 years of age and younger. This study was conducted to determine the food safety knowledge, attitudes, and behaviors of the main food preparer in these families by collecting quantitative and qualitative data simultaneously in a mixed method approach. A food safety knowledge survey created using FightBAC!™ concepts was administered prior to focus groups discussions held in Native American communities using a script based upon the Health Belief Model. Quantitative data were …

Individual Responses For Muscle Activation, Repetitions, And Volume During Three Sets To Failure Of High- (80% 1rm) Versus Low-Load (30% 1rm) Forearm Flexion Resistance Exercise, Nathaniel D.M. Jenkins, Terry J. Housh, Samuel L. Buckner, Haley C. Bergstrom, Kristen C. Cochrane, Cory M. Smith, Ethan C. Hill, Richard J. Schmidt, Joel T. Cramer

Department of Nutrition and Health Sciences: Faculty Publications

This study compared electromyographic (EMG) amplitude, the number of repetitions completed, and exercise volume during three sets to failure of high- (80% 1RM) versus low-load (30% 1RM) forearm flexion resistance exercise on a subject-by-subject basis. Fifteen men were familiarized, completed forearm flexion 1RM testing. Forty-eight to 72 h later, the subjects completed three sets to failure of dumbbell forearm flexion resistance exercise with 80% (n = 8) or 30% (n = 7) 1RM. EMG amplitude was calculated for every repetition, and the number of repetitions performed and exercise volume were recorded. During sets 1, 2, and 3, one of eight …

Knowledge, Use And Perceptions Of Probiotics And Prebiotics In Hospitalized Patients, Melanie Betz, Anne Uzueta, Heather Rasmussen, Mary Gregoire, Cassandra Vandrwall, Gretchen Witowich

Department of Nutrition and Health Sciences: Faculty Publications

Aim: Little is known about inpatient probiotic and prebiotic consumption or beliefs, despite their increase in availability. Therefore, the purpose of this research was to assess inpatient knowledge, use and perceptions of probiotics and prebiotics.

Methods: All subjects were inpatients at two urban medical centers on general medical/surgical floors. Patients were randomly selected to complete a verbally administered questionnaire inquiring about probiotic and prebiotic knowledge, use and perceptions. Patient responses were recorded directly into Survey Monkey (Palo Alto, CA, USA) on a computer.

Results: Patients (n = 200) were 58% were women and 56% were Caucasian with a mean age …

Aerobic Exercise Training Prevents Heart Failure-Induced Skeletal Muscle Atrophy By Anti-Catabolic, But Not Anabolic Actions, Rodrigo W.A. Souza, Warlen P. Piedade, Luana C. Soares, Paula A. T. Souza, Andreo F. Aguiar, Ivan Jose Vechetti Jr, Dijon H.S. Campos, Ana A. H. Fernandes, Katashi Okoshi, Robson F. Carvalho, Antonio C. Cicogna, Maeli Dal-Pai-Silva

Department of Nutrition and Health Sciences: Faculty Publications

Background: Heart failure (HF) is associated with cachexia and consequent exercise intolerance. Given the beneficial effects of aerobic exercise training (ET) in HF, the aim of this study was to determine if the ET performed during the transition from cardiac dysfunction to HF would alter the expression of anabolic and catabolic factors, thus preventing skeletal muscle wasting. Methods and Results: We employed ascending aortic stenosis (AS) inducing HF in Wistar male rats. Controls were sham operated animals. At 18 weeks after surgery, rats with cardiac dysfunction were randomized to 10 weeks of aerobic ET (AS-ET) or to an untrained group …

Knowledge Of And Accordance With The Academy Evidence-Based Nutrition Practice Guideline For Disorders Of Lipid Metabolism, Megan T. Metropulos, Mary B. Gregoire, Sarah J. Holland, Annabelle S. Volgman, Heather E. Rasmussen

Department of Nutrition and Health Sciences: Faculty Publications

The purpose of this study was to determine knowledge of and accordance with practices recommended by the Academy of Nutrition and Dietetics Guideline for Disorders of Lipid Metabolism. Knowledge and food frequency questionnaires were completed by dyslipidemic patients from an outpatient cardiology clinic (n = 51). Participants were primarily white (74.5%), obese (64.7%), female (64.7%) adults 62.0 ± 9.3 years of age. Mean Disorders of Lipid Metabolism guideline knowledge score was 53.l ± 13.4% correct. Mean accordance was 35.4 ± 13.0% correct. There were no significant associations between knowledge and accordance (r_s = 0.175, P = .219). In …

Feeding Condensed Distillers’ Solubles To Feedlot Finishing Steers And The Effects Of Feed Additives In Adaptation Diets, Marie Elizabeth Harris

Department of Animal Science: Dissertations, Theses, and Student Research

By-products from the dry-milling ethanol process can be used in cattle diets to replace corn. There is a significant interaction between corn processing methods as CCDS concentration increases in the diet. Improvements are observed for SFC in final BW, ADG, and G:F over DRC. An experiment was conducted to determine if greater concentrations of CCDS could be fed in SFC based diets and maintain or improve performance. Performance and carcass characteristics were evaluated with increasing concentrations of CCDS at 0, 9, 18, 27, or 36% in place of SFC in feedlot finishing diets. As CCDS concentration increased, DMI decreased quadratically. …

The Environmental And Health Costs Of Alternative Diets: A Comparative Study Of The U. S. Diet Relative To The French, Japanese, Mediterranean, And Nordic Diets, Sarah Rehkamp

Department of Agricultural Economics: Dissertations, Theses, and Student Research

This thesis contributes to the literature on sustainable consumption by using scenario analysis to evaluate the environmental and health costs of the U.S. diet relative to the French, Japanese, Mediterranean, and Nordic diets, identified in the literature as healthier diets. As a first step in estimating environmental costs, the energy efficiencies of each diet are calculated by decomposing each of the diets into their respective components. Then, the dietary efficiencies are translated into CO₂ emissions. As a first step in estimating health costs, a pooled cross-section time-series dataset is used to find the association between BMI and five countries, …

The Association Of Wellness Policy Quality And Percentage Of Obesity In Schools, Bryce M. Abbey

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Schools possess a unique opportunity to reach a large captive audience and are becoming one of the battlegrounds for childhood obesity. To address the school environment’s role on the influence of American children’s nutritional intake and participation in physical activity, the United States (US) Federal Government adopted the Healthy, Hunger-Free Kids Act of 2010, placing an emphasis on implementation of the local school wellness policy (LSW). The purpose of this study was to examine the association between LSW and percentage of obesity in school districts within Nebraska. Aggregate district-wide body mass index (BMI) percentile data were utilized from previously collected …

Feeding Alkaline Treated And Processed Crop Residue To Feedlot Cattle, Sarah Jean Peterson

Department of Animal Science: Dissertations, Theses, and Student Research

Chemical treatment and decreased particle size are methods used to improve digestibility and utilization of the available nutrients in low quality forages. Previous research has indicated that chemically treated corn residue can take the place of corn when included in finishing rations containing distillers grains. Also, decreasing particle size utilizing methods such as pelleting has been shown to improve DMI and ADG. However, limited research has been completed on use of chemical treatment and pelleting in growing and receiving rations. Also, an ideal distillers inclusion has not yet been identified when including alkaline treated stalks in finishing rations. Therefore, a …

Peer Health Teaching Improves Nutrition Behaviors In The Teen Teacher Population, Ashlie Smith

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Peer teaching is a promising model of health education. Limited research exists on the topic of the effectiveness of peer health teaching, and even less research exists on the effects on the actual peer teachers, notably their motivations for becoming peer teachers as well as behavioral modifications and improved self-efficacy made as a result. This study examined the motivations and the health behavior modifications made by 4-H Eat 4-Health teen teachers ages 14 to 17 after delivering a peer health education program. This study investigated teen teachers’ nutrition and physical activity behavior changes as well as their leadership and confidence …

Erratum To ‘‘Associations Of Cardiorespiratory Fitness And Fatness With Metabolic Syndrome In Rural Women With Prehypertension’’, Patricia A. Hageman, Carol H. Pullen, Melody Hertzog, Linda Boeckner, Susan Noble Walker

Department of Nutrition and Health Sciences: Faculty Publications

In the original paper, the authors discovered a computer coding error that resulted in 33 of the women’s ages being incorrectly recorded. All analyses were repeated for this paper using the corrected age dataset, as all our logistic regression analyses in the published paper were adjusted for age.The repeated analyses, using the corrected dataset, lead to minor changes that needed to be reported to the results in the published paper. These corrections did not change the conclusion of the published paper.The authors apologize for any inconvenience.

From Histone Modification To Gene Repression: Epigenetic Regulation By Holocarboxylase Synthetase-Containing Repression Complex And Fad-Dependent Lysine Specific Demethylase, Dandan Liu

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Histones undergo different types of post-translational modifications that have been linked to a variety of biological processes and disease states. Histone acetylation and methylation are well characterized modifications with respect to regulation of chromatin structure and transcription. Histone acetylation, regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs), is linked with gene activation. Here we introduce a novel paradigm suggesting that holocarboxylase synthetase (HLCS), the sole biotin protein ligase in the human genome, recruits nuclear receptor corepressor (N-CoR) and HDAC1 to chromatin, thereby contributing toward the removal of lysine (K)-9 acetylated histone H3 (H3K9ac) gene activation marks and the repression …

Health Care Providers’ Knowledge, Perceptions, And Use Of Probiotics And Prebiotics, Laura Oliver, Heather Rasmussen, Mary B. Gregoire, Yimin Chen

Department of Nutrition and Health Sciences: Faculty Publications

Health care providers (N = 256) completed an online questionnaire to assess their knowledge, perceptions, and use of probiotics and prebiotics. Participants were familiar with probiotics (88%) but not with prebiotics (22%). Probiotics (62%) and prebiotics (55%) were perceived as being “somewhat” to “quite a bit” beneficial to health (μ = 3.6 ± 1.0 and 3.6 ± 1.2, respectively). Health care providers were “quite a bit” to “very much” willing to recommend probiotics (77%) and prebiotics (83%) if substantiated with literature. Despite this belief, they did not recommend probiotics (45%) or prebiotics (26%) to patients or read current research …

Gastrointestinal Problems In Children With Autism, Developmental Delays Or Typical Development, Virginia Chaidez, Robin L. Hansen, Irva Hertz-Picciotto

Department of Nutrition and Health Sciences: Faculty Publications

Abstract To compare gastrointestinal (GI) problems among children with: (1) autism spectrum disorder (ASD), (2) developmental delay (DD) and (3) typical development (TD), GI symptom frequencies were obtained for 960 children from the CHildhood Autism Risks from Genetics and Environment (CHARGE) study. We also examined scores on five Aberrant Behavior Checklist (ABC) subscales comparing ASD children with high versus low frequency GI symptoms. Compared to TD children, those with ASD [aOR 7.92 (4.89–12.85)] and DD [aOR 4.55 (2.51–8.24)] were more likely to have at least one frequent GI symptom. Restricting to ASD children, those with frequent abdominal pain, gaseousness, diarrhea, …