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Characterization Of Human 82-Kda Choline Acetyltransferase Expression In A Newly Developed Transgenic Mouse Model, Silke M. Vanvaerenbergh

Electronic Thesis and Dissertation Repository

Expression of human 82-kDa choline acetyltransferase (ChAT) protein in nuclei of cultured neurons from Alzheimer’s disease model APP/PS1 transgenic mice results in a reduction in Aβ release, suggesting a protective role. This thesis characterizes the expression of human 82-kDa ChAT in 82-hChAT;NkCre transgenic mice by PCR-based examination of genomic DNA and localization of the protein in mice brains. First, I demonstrate in a cultured cell model that Cre recombinase-mediated excision of a floxed LacZ gene is necessary for human 82-kDa ChAT protein expression from pcCALL2:82-ChAT, the plasmid used to create founder transgenic mice. Second, I confirmed that human 82-kDa ChAT …