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Full-Text Articles in Neuroscience and Neurobiology
Resolving Disulfide Bond Patterns In Snap25b Cysteine-Rich Region Using Lc Mass Spectrometry, Nozomi Ogawa
Resolving Disulfide Bond Patterns In Snap25b Cysteine-Rich Region Using Lc Mass Spectrometry, Nozomi Ogawa
Theses and Dissertations
A global analysis of the human proteome demonstrates that there are ~5500 tryptic fragments that contain four cysteines in close proximity. Elucidating whether they form disulfide bonds in vivo under different conditions is particularly important because cysteines are known to be a vital cellular redox sensor as well as a catalytic site for important biochemical reactions. However, currently there are no methods that can resolve disulfide patterns in closely-packed cysteine residues from a complex sample. In order to address this problem, we have developed a novel mass-spectrometry-based method to identify the different disulfide bonding patterns possible, using SNAP25B cysteine-rich region …
The Putative Cannabinoid Receptor Gpr55 Modulates Synaptic Plasticity In The Hippocampus, Corinne Marie Badgley
The Putative Cannabinoid Receptor Gpr55 Modulates Synaptic Plasticity In The Hippocampus, Corinne Marie Badgley
Theses and Dissertations
Endocannabinoids (eCBs) are small molecules that are capable of modulating synaptic plasticity of both excitatory and inhibitory synapses in the brain. While eCBs bind to transient receptor potential vanilloid 1 (TRPV1) and cannabinoid receptor 1 (CB1) in the central nervous system, we recently identified a form of non-CB1, non-TRPV1 mediated long term depression activated by the eCB anandamide at CA1 hippocampal stratum radiatum interneurons. GPR55, an orphan G-protein receptor, has been identified in the hippocampus and is capable of activation by eCBs, making it a good candidate for mediating this non-CB1, non-TRPV1 form of synaptic plasticity. Here we performed whole-cell …
A Parametric Investigation Of Pattern Separation Processes In The Medial Temporal Lobe, Sarah E. Motley
A Parametric Investigation Of Pattern Separation Processes In The Medial Temporal Lobe, Sarah E. Motley
Theses and Dissertations
The hippocampus is thought to be involved in memory formation and consolidation, with computational models proposing the process of pattern separation as a means for encoding overlapping memories. Previous research has used semantically related targets and lures to investigate hippocampal responses to mnemonic interference. Here, we attempted to define the response function of the hippocampus and its inputs during pattern separation by parametrically varying target-lure similarity in a continuous recognition task. We also investigated the effect of task demands (intentional versus incidental encoding) on pattern separation processes. We collected functional magnetic resonance imaging (fMRI) data while participants were shown a …