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Full-Text Articles in Neuroscience and Neurobiology
Transient Suppression Of Dbx1 Prebotzinger Interneurons Disrupts Breathing In Adult Mice, Nikolas C. Vann, Francis D. Pham, John A. Hayes, Andrew Kottick, Christopher A. Del Negro
Transient Suppression Of Dbx1 Prebotzinger Interneurons Disrupts Breathing In Adult Mice, Nikolas C. Vann, Francis D. Pham, John A. Hayes, Andrew Kottick, Christopher A. Del Negro
Arts & Sciences Articles
Interneurons derived from Dbx1-expressing precursors located in the brainstem preBotzinger complex (preBotC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hyperpolarizing these neurons while measuring respiratory rhythm in vitro or breathing in vagus-intact adult mice. Transient illumination of the preBotC interrupted inspiratory rhythm in both slice preparations and sedated mice. In awake mice, light application reduced breathing frequency and prolonged the inspiratory duration. Support for the Dbx1 core hypothesis previously came from embryonic and perinatal mouse experiments, but these data suggest that Dbx1-derived preBotC …
Experimental Exposure To Urban And Pink Noise Affects Brain Development And Song Learning In Zebra Finches (Taenopygia Guttata), Dominique A. Potvin, Michael T. Curcio, John P. Swaddle, Scott A. Macdougall-Shackleton
Experimental Exposure To Urban And Pink Noise Affects Brain Development And Song Learning In Zebra Finches (Taenopygia Guttata), Dominique A. Potvin, Michael T. Curcio, John P. Swaddle, Scott A. Macdougall-Shackleton
Arts & Sciences Articles
Recently, numerous studies have observed changes in bird vocalizations—especially song—in urban habitats. These changes are often interpreted as adaptive, since they increase the active space of the signal in its environment. However, the proximate mechanisms driving cross-generational changes in song are still unknown. We performed a captive experiment to identify whether noise experienced during development affects song learning and the development of song-control brain regions. Zebra finches (Taeniopygia guttata) were bred while exposed, or not exposed, to recorded traffic urban noise (Study 1) or pink noise (Study 2). We recorded the songs of male offspring and compared these …
Functional Interactions Between Mammalian Respiratory Rhythmogenic And Premotor Circuitry, Hanbing Song, John A. Hayes, Nikolas C. Vann, Xueying Wang, Christopher A. Del Negro, M. Drew Lamar
Functional Interactions Between Mammalian Respiratory Rhythmogenic And Premotor Circuitry, Hanbing Song, John A. Hayes, Nikolas C. Vann, Xueying Wang, Christopher A. Del Negro, M. Drew Lamar
Arts & Sciences Articles
Breathing in mammals depends on rhythms that originate from the preBotzinger complex (preBotC) of the ventral medulla and a network of brainstem and spinal premotor neurons. The rhythm-generating core of the preBotC, as well as some premotor circuits, consist of interneurons derived from Dbx1-expressing precursors (Dbx1 neurons), but the structure and function of these networks remain incompletely understood. We previously developed a cell-specific detection and laser ablation system to interrogate respiratory network structure and function in a slice model of breathing that retains the preBotC, the respiratory-related hypoglossal (XII) motor nucleus and XII premotor circuits. In spontaneously rhythmic slices, cumulative …
Organotypic Slice Cultures Containing The Prebotzinger Complex Generate Respiratory-Like Rhythms, Wiktor S. Phillips, Mikkel Herly, Jens C. Rekling, Christopher A. Del Negro
Organotypic Slice Cultures Containing The Prebotzinger Complex Generate Respiratory-Like Rhythms, Wiktor S. Phillips, Mikkel Herly, Jens C. Rekling, Christopher A. Del Negro
Arts & Sciences Articles
Study of acute brain stem slice preparations in vitro has advanced our understanding of the cellular and synaptic mechanisms of respiratory rhythm generation, but their inherent limitations preclude long-term manipulation and recording experiments. In the current study, we have developed an organotypic slice culture preparation containing the preBotzinger complex (preBotC), the core inspiratory rhythm generator of the ventrolateral brain stem. We measured bilateral synchronous network oscillations, using calcium-sensitive fluorescent dyes, in both ventrolateral (presumably the preBotC) and dorsomedial regions of slice cultures at 7-43 days in vitro. These calcium oscillations appear to be driven by periodic bursts of inspiratory neuronal …