Environmental Microbiology and Microbial Ecology Commons^™

Bacterioplankton Growth Responses To Temperature And Chlorophyll Variations In Estuaries Measured By Thymidine:Leucine Incorporation Ratio, Fk Shiah, Hw Ducklow

VIMS Articles

To identify the biochemical response of heterotrophic bacterioplankton to changing environmental conditions, seasonal and diel cycles of bacterial protein and DNA synthesis rates were estimated in temperate estuarine habitats from H-3-leucine (Leu) and H-3-thymidine (TdR) incorporation rates. Several short-term temperature manipulation experiments (5 to 35 degrees C) and 2 mesocosm experiments were performed to examine the effects of temperature and substrate supply on the ratio of Leu:TdR, respectively. The molar ratio of Leu to TdR varied about 5-fold (5.6 to 29.5) in the field and the values of the ratio were lower and more constant during high temperature (>25 …