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Full-Text Articles in Environmental Microbiology and Microbial Ecology
Improved Methods For The Quantification Of Viable Ascaris Suum And Application To Biosolids, Tanner Keyzers
Improved Methods For The Quantification Of Viable Ascaris Suum And Application To Biosolids, Tanner Keyzers
Dissertations, Master's Theses and Master's Reports
The current method for detection of Ascaris ova in biosolids and wastewater has several drawbacks including being labor and time intensive as well as being difficult to quantify viable ova that have larvated during treatment. The goal of this thesis was to improve several methods for the detection and quantification of Ascaris ova during long term-storage of biosolids including improvement of quantifying viable ova using a heat and bleach treatment to promote movement of larvae inside of ova, and comparison of the traditional microscopic method with qPCR and PMA-qPCR to prevent the false-positive results. Improved methods to promote movement of …
Microbial Repopulation Following In Situ Star Remediation, Gavin Overbeeke
Microbial Repopulation Following In Situ Star Remediation, Gavin Overbeeke
Electronic Thesis and Dissertation Repository
In situ STAR (Self-sustaining Treatment for Active Remediation) is an emerging remediation technology which uses smouldering combustion to destroy nonaqueous phase liquid (NAPL) contamination in the subsurface. Since STAR smouldering travels through contaminated soils slowly (~0.5 to 5 m/day) and subjects them to high temperatures (400–1000°C), it is expected that this technology will thoroughly dry and sterilize the zones which it treats. Further, soils surrounding the treatment zone which are not smouldered will be heated, although not smouldered, by virtue of their proximity to STAR, impacting microbial communities within them. Therefore, the objectives of this work are to quantify the …