Open Access. Powered by Scholars. Published by Universities.®
Environmental Microbiology and Microbial Ecology Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Environmental Microbiology and Microbial Ecology
Evaluation Of Qpcr To Detect Shifts In Population Composition Of The Rhizobial Symbiont Mesorhizobium Japonicum During Serial In Planta Transfers, Kenjiro W. Quides, Yoobeen Lee, Teresa Hur, Hagop S. Atamian
Evaluation Of Qpcr To Detect Shifts In Population Composition Of The Rhizobial Symbiont Mesorhizobium Japonicum During Serial In Planta Transfers, Kenjiro W. Quides, Yoobeen Lee, Teresa Hur, Hagop S. Atamian
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Microbial symbionts range from mutualistic to commensal to antagonistic. While these roles are distinct in their outcome, they are also fluid in a changing environment. Here, we used the Lotus japonicus–Mesorhizobium japonicum symbiosis to investigate short-term and long-term shifts in population abundance using an effective, fast, and low-cost tracking methodology for M. japonicum. We use quantitative polymerase chain reaction (qPCR) to track previously generated signature-tagged M. japonicum mutants targeting the Tn5 transposon insertion and the flanking gene. We used a highly beneficial wild type and moderately beneficial and non-beneficial mutants of M. japonicum sp. nov. to demonstrate the specificity …