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Environmental Microbiology and Microbial Ecology Commons™
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Full-Text Articles in Environmental Microbiology and Microbial Ecology
Symbiotic Ucyn-A Strains Co-Occurred With El Niño, Relaxed Upwelling, And Varied Eukaryotes Over 10 Years Off Southern California, Colette Fletcher-Hoppe, Yi-Chun Yeh, Yubin Raut, J. L. Weissman, Jed A. Fuhrman
Symbiotic Ucyn-A Strains Co-Occurred With El Niño, Relaxed Upwelling, And Varied Eukaryotes Over 10 Years Off Southern California, Colette Fletcher-Hoppe, Yi-Chun Yeh, Yubin Raut, J. L. Weissman, Jed A. Fuhrman
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Biological nitrogen fixation, the conversion of N2 gas into a bioavailable form, is vital to sustaining marine primary production. Studies have shifted beyond traditionally studied tropical diazotrophs. Candidatus Atelocyanobacterium thalassa (or UCYN-A) has emerged as a focal point due to its streamlined metabolism, intimate partnership with a haptophyte host, and broad distribution. Here, we explore the environmental parameters that govern UCYN-A’s presence at the San Pedro Ocean Time-series (SPOT), its host specificity, and statistically significant interactions with non-host eukaryotes from 2008-2018. 16S and 18S rRNA gene sequences were amplified by “universal primers” from monthly samples and resolved into Amplicon …
Evaluation Of Qpcr To Detect Shifts In Population Composition Of The Rhizobial Symbiont Mesorhizobium Japonicum During Serial In Planta Transfers, Kenjiro W. Quides, Yoobeen Lee, Teresa Hur, Hagop S. Atamian
Evaluation Of Qpcr To Detect Shifts In Population Composition Of The Rhizobial Symbiont Mesorhizobium Japonicum During Serial In Planta Transfers, Kenjiro W. Quides, Yoobeen Lee, Teresa Hur, Hagop S. Atamian
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Microbial symbionts range from mutualistic to commensal to antagonistic. While these roles are distinct in their outcome, they are also fluid in a changing environment. Here, we used the Lotus japonicus–Mesorhizobium japonicum symbiosis to investigate short-term and long-term shifts in population abundance using an effective, fast, and low-cost tracking methodology for M. japonicum. We use quantitative polymerase chain reaction (qPCR) to track previously generated signature-tagged M. japonicum mutants targeting the Tn5 transposon insertion and the flanking gene. We used a highly beneficial wild type and moderately beneficial and non-beneficial mutants of M. japonicum sp. nov. to demonstrate the specificity …