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Articles 1 - 7 of 7
Full-Text Articles in Microbiology
The Roles Of Biotin In Candida Albicans Physiology, Nur Ras Aini Ahmad Hussin
The Roles Of Biotin In Candida Albicans Physiology, Nur Ras Aini Ahmad Hussin
School of Biological Sciences: Dissertations, Theses, and Student Research
Due to the increased number of immunocompromised patients, infections by Candida albicans have significantly increased in recent years. C. albicans transition from yeast to germ tubes is an essential factor for virulence. In this study we noted that Lee's medium, commonly used to induce filamentation, contained 500-fold more biotin than needed for growth. Thus, we investigated the effects of excess biotin on growth rate and filamentation by C. albicans in different media. At 37 °C, excess biotin (4 µM) enhanced germ tube formation (GTF) ca. 10-fold in both Lee's medium and a defined glucose proline medium, and ca. 4-fold in …
Physiological Characterization Of Prochlorococcus Under Abiotic Stressors Temperature And Hydrogen Peroxide, Lanying Ma
Physiological Characterization Of Prochlorococcus Under Abiotic Stressors Temperature And Hydrogen Peroxide, Lanying Ma
Doctoral Dissertations
Cyanobacteria of the genus Prochlorococcus are the smallest and most abundant phytoplankters in the ocean. Temperature is a major influence on Prochlorococcus abundance and distribution in the ocean, but the physiological basis for this relationship is not well understood. In other microbes, lipid and fatty acid composition have been shown to be influenced by temperature, and temperature has also been proposed as a relevant factor for setting the elemental allocation in marine phytoplankton. In this study, we found that percentage of fatty acids unsaturation was negatively related with temperature in some Prochlorococcus strains, but this was not universal. Temperature had …
The Roles Of Microcystin And Sulfide In Physiology And Tactic Responses Of Pathogenic And Non-Pathogenic Mat-Forming Cyanobacteria, Abigael C. Brownell
The Roles Of Microcystin And Sulfide In Physiology And Tactic Responses Of Pathogenic And Non-Pathogenic Mat-Forming Cyanobacteria, Abigael C. Brownell
FIU Electronic Theses and Dissertations
Planktothricoides raciborskii and Roseofilum reptotaenium are physiologically similar, yet ecologically distinct organisms found in a hot spring outflow and coral black band disease (BBD), respectively. The aim of this study was to elucidate the relationship between R. reptotaenium and sulfide in BBD, to compare microcystin (MC) production in response to environmental factors, and to determine chemotactic responses to MC and sulfide by the two organisms. Results showed that the pathogenicity of R. reptotaenium in BBD is dependent on sulfate-reducing bacteria as secondary pathogens. Roseofilum reptotaenium produced significantly more MC than P. raciborskii, as measured using ELISA. Roseofilum reptotaenium …
Characterization Of Phycoerythrin Physiology In Low-Light Adapted Prochlorococcus Ecotypes, Kathryn H. Roache-Johnson
Characterization Of Phycoerythrin Physiology In Low-Light Adapted Prochlorococcus Ecotypes, Kathryn H. Roache-Johnson
Electronic Theses and Dissertations
The marine cyanobacteria Prochlorococcus and Synechococcus are the most abundant phototrophs in the oceans. They cohabit the oligotrophic ocean and thus have coevolved together, yet they have distinctly different methods for harvesting light. Synechococcus, like other cyanobacteria, possess phycobilisomes with various combinations of phycobiliproteins to capture wavelengths of light not otherwise available to chlorophyll. Prochlorococcus lack phycobilisomes and use divinyl chlorophyll b (Chl b2) as their primary accessory pigment to divinyl chlorophyll a (Chl a2) to capture light energy. In addition to the divinyl chlorophylls, Prochlorococcus has genes associated with the phycobiliprotein phycoerythrin (PE), the role of which is still …
The Armadillo Repeat Protein Pf16 Is Essential For Flagellar Structure And Function In Plasmodium Male Gametes, Ursula Straschil, Arthur M. Talman, David J. P. Ferguson, Karen A. Bunting, Zhengyao Xu, Elizabeth Bailes, Robert E. Sinden, Anthony A. Holder, Elizabeth F. Smith
The Armadillo Repeat Protein Pf16 Is Essential For Flagellar Structure And Function In Plasmodium Male Gametes, Ursula Straschil, Arthur M. Talman, David J. P. Ferguson, Karen A. Bunting, Zhengyao Xu, Elizabeth Bailes, Robert E. Sinden, Anthony A. Holder, Elizabeth F. Smith
Dartmouth Scholarship
Malaria, caused by the apicomplexan parasite Plasmodium, threatens 40% of the world's population. Transmission between vertebrate and insect hosts depends on the sexual stages of the life-cycle. The male gamete of Plasmodium parasite is the only developmental stage that possesses a flagellum. Very little is known about the identity or function of proteins in the parasite's flagellar biology. Here, we characterise a Plasmodium PF16 homologue using reverse genetics in the mouse malaria parasite Plasmodium berghei. PF16 is a conserved Armadillo-repeat protein that regulates flagellar structure and motility in organisms as diverse as green algae and mice. We show that …
Parasites Alter Community Structure, Chelsea L. Wood, James E. Byers, Kathryn L. Cottingham, Irit Altman
Parasites Alter Community Structure, Chelsea L. Wood, James E. Byers, Kathryn L. Cottingham, Irit Altman
Dartmouth Scholarship
Parasites often play an important role in modifying the physiology and behavior of their hosts and may, consequently, mediate the influence hosts have on other components of an ecological community. Along the northern Atlantic coast of North America, the dominant herbivorous snail Littorina littorea structures rocky intertidal communities through strong grazing pressure and is frequently parasitized by the digenean trematode Cryptocotyle lingua. We hypothesized that the effects of parasitism on host physiology would induce behavioral changes in L. littorea, which in turn would modulate L. littorea's influence on intertidal community composition. Specifically, we hypothesized that C. lingua …
Susceptibility Of Biofilms To Bdellovibrio Bacteriovorus Attack, Daniel Kadouri, George A. O'Toole
Susceptibility Of Biofilms To Bdellovibrio Bacteriovorus Attack, Daniel Kadouri, George A. O'Toole
Dartmouth Scholarship
Biofilms are communities of microorganisms attached to a surface, and the growth of these surface attached communities is thought to provide microorganisms with protection against a range of biotic and abiotic agents. The capability of the gram-negative predatory bacterium Bdellovibrio bacteriovorus to control and reduce an existing Escherichia coli biofilm was evaluated in a static assay. A reduction in biofilm biomass was observed as early as 3 h after exposure to the predator, and an 87% reduction in crystal violet staining corresponding to a 4-log reduction in biofilm cell viability was seen after a 24-h exposure period. We observed that …