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Full-Text Articles in Microbiology

Controls On Benthic Microbial Community Structure And Assembly In A Karstic Coastal Wetland, Nicholas O. Schulte Mar 2016

Controls On Benthic Microbial Community Structure And Assembly In A Karstic Coastal Wetland, Nicholas O. Schulte

FIU Electronic Theses and Dissertations

The assembly mechanisms underlying microbial community abundance, biotic interactions, and diversity over space and time are unresolved, particularly in benthic microbial mats distributed along environmental gradients. Experimental enrichment of nutrient-limited microbial mats from the Florida Everglades along a nutrient subsidy-salinity stress gradient stimulated autotrophic and heterotrophic metabolism, growth, and diversity independent of autotroph-heterotroph interactions across treatments and space. These results suggest spatial segregation of autotrophic and heterotrophic components within mats. Considering only the diatom component of Everglades mats over space and time, the subsidy-stress gradient controlled diatom compositional turnover at broad spatial scales while environmental and dispersal-based processes structured diatom …


Metabolism Of Herpes Simplex Virus 1 Infected Raw 264.7 Macrophages, Mary K. Jenkins Jan 2016

Metabolism Of Herpes Simplex Virus 1 Infected Raw 264.7 Macrophages, Mary K. Jenkins

Browse all Theses and Dissertations

Macrophages are immune cells that phagocytize pathogens and act as antigen presenting cells. Macrophages are critical in regulating the adaptive immune response and play a key role in neutralizing infection. Macrophages exhibit a variety of phenotypes which have different energy requirements. In the case of HSV-1 infection the virus has been shown to alter metabolic processes of its host. Utilizing a XF24 Extracellular Flux Analyzer the metabolic activity of M0, M1, and M2 (IL-4, IL-13, and IL-10) RAW 264.7 macrophages was quantified for both uninfected cells and in response to HSV-1 infection. The analysis showed uninfected M0 and M2 (IL-4, …


Changes In Cytoskeleton Proteins In Hsv-1 Infection Of J774a.1 Macrophage Phenotype, Riham Abbas Subahi Jan 2016

Changes In Cytoskeleton Proteins In Hsv-1 Infection Of J774a.1 Macrophage Phenotype, Riham Abbas Subahi

Browse all Theses and Dissertations

The cell cytoskeleton, a unique intracellular matrix found in all eukaryotes, is composed of three main protein structures, microtubules, microlaments and intermediate laments. The cytoskeleton maintains cell shape and internal structures providing mechanical support that facilitates intracellular transport (Parker et al., 2014). Many viruses such as the herpesviruses use the cytoskeleton system of the cell for infectivity (Henry Sum, M. S. 2015). HSV-1 utilizes the cell cytoskeleton in many steps of its life cycle from entry through assembly to egress (Lyman et al., 2008). During infection, HSV-1 viral proteins cause drastic changes and rearrangement of the cellular actin and cell …


The Impact Of Urbanization On Mosquito-Borne Viruses, Nikhita Puthuveetil Jan 2016

The Impact Of Urbanization On Mosquito-Borne Viruses, Nikhita Puthuveetil

Undergraduate Research Posters

Mosquito-borne diseases such as yellow fever, dengue, and West Nile are rapidly emerging across the globe. Their emergence is often aided by the growth of their vector population, or the organisms that transmit the virus to the host. Urbanization and land use often destroys the habitat of the virus and its vector. However, the virus and its vector often survive despite the changes to its environment. The goal of this paper is to find out exactly how urbanization and changes in land use affect mosquito-borne viruses and how these viruses survive despite the destruction of their habitats. To understand how …