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Searching For Crp: Investigating Candidate Genes For The Camp Receptor Protein In Bdellovibrio Bacteriovorus, Kat Schmidt
Searching For Crp: Investigating Candidate Genes For The Camp Receptor Protein In Bdellovibrio Bacteriovorus, Kat Schmidt
Summer Research
Bdellovibrio bacteriovorus is a δ-proteobacter with an obligatory predatory lifestyle, consuming a wide variety of Gram-negative bacteria. Its unusual life cycle and genome make it a particularly interesting organism to study. In this experiment, three candidate crp homologs – Bd0446, Bd2590, and Bd2602 – were evaluated by complementation studies in Escherichia coli, and characterized by bioinformatic analysis. Based on the complementation studies, none of these genes function as a crp homolog in E. coli. However, comparative bioinformatic analysis strongly suggests that either Bd2590 or Bd2602 may indeed code for the cyclic AMP receptor protein in Bdellovibrio, and merit further investigation. …