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Full-Text Articles in Microbiology

Antimicrobial Activity Screening Of Recombinant And Synthetic Varasin A Defensin From The Hard Tick Dermacentor Variabilis Against Various Bacteria, Julia A. Sharp Oct 2007

Antimicrobial Activity Screening Of Recombinant And Synthetic Varasin A Defensin From The Hard Tick Dermacentor Variabilis Against Various Bacteria, Julia A. Sharp

Biological Sciences Theses & Dissertations

Hematophagous arthropods, such as ticks and mosquitoes, rely on their innate immune system for defense against pathogens ingested in a blood meal as well as those acquired through injury. In response to pathogen recognition, the production of antimicrobial peptides, such as defensin, is typically upregulated. Varisin, a defensin, is thought to be a key component in the immunocompetence of the hard tick D. variabilis against Borrelia burgdorferi, the causative agent of Lyme disease. To study the antimicrobial effects of varisin, recombinant varisin was expressed by both insect cells and E coli. Purification of the protein followed by enterokinase treatment yielded …


Conservation Of The Sos Operon, Umudc, In Acinetobacter Species, Ching-Li Lin May 2007

Conservation Of The Sos Operon, Umudc, In Acinetobacter Species, Ching-Li Lin

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science & Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Ching-Li Lin on May 1, 2007.


Bactericidal Effects Of Cold Plasma Technology On Geobacillus Stearothermophilus And Bacillus Cereus Microorganisms, Angela Dawn Morris Apr 2007

Bactericidal Effects Of Cold Plasma Technology On Geobacillus Stearothermophilus And Bacillus Cereus Microorganisms, Angela Dawn Morris

Dental Hygiene Theses & Dissertations

Non-equilibrium atmospheric pressure plasma, also known as cold plasma, is a state of matter that consists of a mix of neutral and charged particles. Plasma generates chemically reactive species and ultraviolet radiation making them useful in decontamination applications (Kong & Laroussi, 2003). Research regarding the inactivation of gram-positive bacteria, such as Bacillus atrophaeus, by cold plasma has been studied by Laroussi et al. (2003); however, there is limited research regarding the germicidal effectiveness of cold plasma on the microorganisms Geobacillus stearothermophilus and Bacillus cereus. The purpose of this study was to determine if cold plasma technology inactivates heat …


Aislamiento E Identificación De Bacterias Ácido Lácticas A Partir De Leche Cruda Y Queso Paipa Elaborado En Los Municipios De Pacho Cundinamarca Y Belén Boyacá, Gladys Lorena Rodríguez Villanueva Jan 2007

Aislamiento E Identificación De Bacterias Ácido Lácticas A Partir De Leche Cruda Y Queso Paipa Elaborado En Los Municipios De Pacho Cundinamarca Y Belén Boyacá, Gladys Lorena Rodríguez Villanueva

Zootecnia

La fermentación de los alimentos por bacterias ácido lácticas es una de las formas más antiguas de conservación usadas por el hombre. Uno de los alimentos resultante de dichas fermentaciones es el queso Paipa, el cual es uno de los productos mas representativos de nuestro país en cuanto a quesos semimadurados, desafortunadamente, este tipo de queso se produce en forma artesanal, haciéndolo riesgoso para la salud pública, debido a que la leche utilizada en este proceso no es sometida a tratamiento térmico. En el presente trabajo, se realizaron análisis físico-químicos a la leche y al queso Paipa identificándolo como un …


Evaluation Of Four Novel Broad-Host-Range Antimicrobial Agents., Jiahui Guo Jan 2007

Evaluation Of Four Novel Broad-Host-Range Antimicrobial Agents., Jiahui Guo

Theses

The overall objective of this thesis was to evaluate the antimicrobial effects of four novel broad-host-range antimicrobial agents. The first was a milk-derived lipoprotein developed by Westgate Biologicals with the support of Enterprise Ireland. This lipoprotein which was designated LactiSAL, was evaluated against a wide range of bacterial species associated with humans. Essentially, the LactiSAL was incorporated at a variety of concentrations into agar media, which were appropriate for cultivation of the different genera. Agars were examined for colony formation, after which it was possible to quantify the minimal inhibitory concentration for each genus. Subsequently, LactiSAL was incorporated at levels …