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Full-Text Articles in Microbiology
Genomic Organization Of Infectious Salmon Anemia Virus, Trent Rector
Genomic Organization Of Infectious Salmon Anemia Virus, Trent Rector
Electronic Theses and Dissertations
Infectious salmon anemia virus (ISAV) is an emerging pathogen of farmed Atlantic salmon (Salmo salar). The development of an effective ISA virus vaccine is a high priority for salmon producers in the U.S. and elsewhere. The process of developing a recombinant vaccine requires complete genetic characterization of the virus. Toward this end we have cloned, sequenced and determined the organization of the eight segments of single-stranded RNA from ISA virus isolate CCBB. The virus was grown in cell culture and purified by density gradient ultracentrifugation. Viral RNA was isolated from purified ISAV and used in the construction of two different …