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Articles 1 - 4 of 4
Full-Text Articles in Microbiology
Genomic Organization Of Infectious Salmon Anemia Virus, Trent Rector
Genomic Organization Of Infectious Salmon Anemia Virus, Trent Rector
Electronic Theses and Dissertations
Infectious salmon anemia virus (ISAV) is an emerging pathogen of farmed Atlantic salmon (Salmo salar). The development of an effective ISA virus vaccine is a high priority for salmon producers in the U.S. and elsewhere. The process of developing a recombinant vaccine requires complete genetic characterization of the virus. Toward this end we have cloned, sequenced and determined the organization of the eight segments of single-stranded RNA from ISA virus isolate CCBB. The virus was grown in cell culture and purified by density gradient ultracentrifugation. Viral RNA was isolated from purified ISAV and used in the construction of two different …
The Mannose-Sensitive Hemagglutinin Of Vibrio Cholerae Promotes Adherence To Zooplankton, Deborah A. Chiavelli, Jane W. Marsh, Ronald K. Taylor
The Mannose-Sensitive Hemagglutinin Of Vibrio Cholerae Promotes Adherence To Zooplankton, Deborah A. Chiavelli, Jane W. Marsh, Ronald K. Taylor
Dartmouth Scholarship
The bacterium Vibrio cholerae, the etiological agent of cholera, is often found attached to plankton, a property that is thought to contribute to its environmental persistence in aquatic habitats. The V. cholerae O1 El Tor biotype and V. cholerae O139 strains produce a surface pilus termed the mannose-sensitive hemagglutinin (MSHA), whereas V. cholerae O1 classical biotype strains do not. Although V. cholerae O1 classical does not elaborate MSHA, the gene is present and expressed at a level comparable to that of the other strains. Since V. cholerae O1 El Tor and V. cholerae O139 have displaced V. cholerae O1 classical …
Canine And Feline Parvoviruses Can Use Human Or Feline Transferrin Receptors To Bind, Enter, And Infect Cells, John S. L. Parker, William J. Murphy, Dai Wang, Stephen J. O'Brien, Colin R. Parrish
Canine And Feline Parvoviruses Can Use Human Or Feline Transferrin Receptors To Bind, Enter, And Infect Cells, John S. L. Parker, William J. Murphy, Dai Wang, Stephen J. O'Brien, Colin R. Parrish
Biology Faculty Articles
Canine parvovirus (CPV) enters and infects cells by a dynamin-dependent, clathrin-mediated endocytic pathway, and viral capsids colocalize with transferrin in perinuclear vesicles of cells shortly after entry (J. S. L. Parker and C. R. Parrish, J. Virol. 74:1919–1930, 2000). Here we report that CPV and feline panleukopenia virus (FPV), a closely related parvovirus, bind to the human and feline transferrin receptors (TfRs) and use these receptors to enter and infect cells. Capsids did not detectably bind or enter quail QT35 cells or a Chinese hamster ovary (CHO) cell-derived cell line that lacks any TfR (TRVb cells). However, capsids bound and …
Investigations Into The Innate Resistance Of The Tick, Dermacentor Variabilis, When Challenged With The Bacterium, Escherichia Coli, Shane Michael Ceraul
Investigations Into The Innate Resistance Of The Tick, Dermacentor Variabilis, When Challenged With The Bacterium, Escherichia Coli, Shane Michael Ceraul
Biological Sciences Theses & Dissertations
In addition to the soluble response, many invertebrates control bacterial infections by means of phagocytosis or melanotic encapsulation. In some insects, Escherichia coli growth is reported to be inhibited by aggregation/encapsulation. Soluble and phagocytic responses to bacterial challenge have been reported in ticks, but evidence of an aggregation/encapsulation response was reported only for inanimate (araldite) implants. This study was done to determine how ticks control infection by E. coli. Ticks were challenged by direct inoculation of bacteria into the hemocoel cavity. Using plate counts, no viable E. coli were detected I hour post-inoculation. A direct fluorescence assay (DF A) …