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Full-Text Articles in Microbiology
Immunological Relationships Among Proteins Making Up The Bacillus Thuringiensis Subsp. Israelensis Crystalline Toxin, Mary Ann Pfannenstiel, Graham A. Couche, Elise J. Ross, Kenneth W. Nickerson
Immunological Relationships Among Proteins Making Up The Bacillus Thuringiensis Subsp. Israelensis Crystalline Toxin, Mary Ann Pfannenstiel, Graham A. Couche, Elise J. Ross, Kenneth W. Nickerson
Papers in Microbiology
The immunological relationships among the proteins of the mosquito larvicidal toxin produced by Bacillus thuringiensis subsp. israelensis have been investigated by using polyclonal antisera specific for the 28-, 70-, and 135-kilodalton proteins. Each of these proteins was immunologically distinct. There was no cross-reaction among the three proteins and the two non-homologous antisera. Treatment of toxin proteins with larval gut enzymes for 20 h identified protease-resistant domains at approximately 65, 38, and 22 kilodaltons. Similar domains were generated by treatment with trypsin and chymotrypsin. Our immunological and kinetic data indicate that the 28-kilodalton protein is degraded successively to protein bands at …
Analysis Of Mosquito Larvicidal Potential Exhibited By Vegetative Cells Of Bacillus Thuringiensis Subsp. Israelensis, Corey J. Walther, Graham A. Couche, Mary Ann Pfannenstiel, Sara E. Egan, Lisa A. Bivin, Kenneth W. Nickerson
Analysis Of Mosquito Larvicidal Potential Exhibited By Vegetative Cells Of Bacillus Thuringiensis Subsp. Israelensis, Corey J. Walther, Graham A. Couche, Mary Ann Pfannenstiel, Sara E. Egan, Lisa A. Bivin, Kenneth W. Nickerson
Papers in Microbiology
Vegetative Bacillus thuringiensis subsp. israelensis cells (6 x 105/ml) achieved 100% mortality of Aedes aegypti larvae within 24 h. This larvicidal potential was localized within the cells; the cell-free supernatants did not kill mosquito larvae. However, they did contain a heat-labile hemolysin which was immunologically distinct from the general cytolytic (hemolytic) factor released during solubilization of B. thuringiensis subsp. israelensis crystals. The larvicidal potential of the vegetative cells was not due to poly-3-hydroxybutyrate. Instead, it correlated with the ability of vegetative cells to sporulate during the bioassays. No toxicity was observed when bioassays were conducted in the presence …